Omi/HtrA2 pro-apoptotic marker differs in various hepatocellular carcinoma cell lines owing to ped/pea-15 expression level

  • Authors:
    • Zongquan Xu
    • Yu Chen
    • Guohui Xu
    • Cheng Peng
    • Enyu Liu
    • Yunguang Li
    • Jun Niu
    • Changhai Li
  • View Affiliations

  • Published online on: December 8, 2014     https://doi.org/10.3892/or.2014.3656
  • Pages: 905-912
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Abstract

Omi/HtrA2 promotes cell apoptosis in human cancer cells. Early studies showed that primary hepatocellular carcinoma requires Omi/HtrA2 expression for cell apoptosis. Additionally, the Omi/HtrA2 pro-apoptotic marker demonstrated a difference in some cell types. However, how the Omi/HtrA2 pro-apoptotic marker reacts during the process of hepatocellular carcinoma cell apoptosis remains to be determined. Thus, we investigated the role and possible mechanism of Omi/HtrA2 on hepatocellular carcinoma cell apoptosis using various hepatocellular carcinoma cell lines. The results were analyzed using RT‑qPCR and western blot analysis. In the present study, we found that Omi/HtrA2 was overexpressed in hepatocellular carcinoma cell lines and induced hepatocellular carcinoma cell apoptosis. Additiionally, the only manner in which Omi/HtrA2 participated in cell death in PLC cells may be dependent on IAP-binding. Omi/HtrA2‑inducing HepG2 cell apoptosis may mainly depend on its serine protease activity while both IAP-binding and its serine protease activity participated in Hep3B cell apoptosis. This result suggested that Omi/HtrA2 pro-apoptotic marker differs in various hepatocellular carcinoma cell lines. PLC cells were also devoid of the expression of ped/pea-15 as the substrate of Omi/HtrA2 serine protease while ped/pea-15 was overexpressed in HepG2 and Hep3B cells and ped/pea-15 expression was higher in HepG2 cells than that in Hep3B cells. These results showed that Omi/HtrA2 overexpression promotes hepatocellular carcinoma cell apoptosis and the ped/pea-15 expression level causes this difference of the Omi/HtrA2 pro-apoptotic marker in the various hepatocellular carcinoma cell lines.
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February-2015
Volume 33 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xu Z, Chen Y, Xu G, Peng C, Liu E, Li Y, Niu J and Li C: Omi/HtrA2 pro-apoptotic marker differs in various hepatocellular carcinoma cell lines owing to ped/pea-15 expression level. Oncol Rep 33: 905-912, 2015
APA
Xu, Z., Chen, Y., Xu, G., Peng, C., Liu, E., Li, Y. ... Li, C. (2015). Omi/HtrA2 pro-apoptotic marker differs in various hepatocellular carcinoma cell lines owing to ped/pea-15 expression level. Oncology Reports, 33, 905-912. https://doi.org/10.3892/or.2014.3656
MLA
Xu, Z., Chen, Y., Xu, G., Peng, C., Liu, E., Li, Y., Niu, J., Li, C."Omi/HtrA2 pro-apoptotic marker differs in various hepatocellular carcinoma cell lines owing to ped/pea-15 expression level". Oncology Reports 33.2 (2015): 905-912.
Chicago
Xu, Z., Chen, Y., Xu, G., Peng, C., Liu, E., Li, Y., Niu, J., Li, C."Omi/HtrA2 pro-apoptotic marker differs in various hepatocellular carcinoma cell lines owing to ped/pea-15 expression level". Oncology Reports 33, no. 2 (2015): 905-912. https://doi.org/10.3892/or.2014.3656