Thioredoxin-1 inhibitor, 1-methylpropyl 2-imidazolyl disulfide, inhibits the growth, migration and invasion of colorectal cancer cell lines

  • Authors:
    • Fule Wang
    • Feiyan Lin
    • Peili Zhang
    • Wuhua Ni
    • Laixi Bi
    • Jianbo Wu
    • Lei Jiang
  • View Affiliations

  • Published online on: December 5, 2014     https://doi.org/10.3892/or.2014.3652
  • Pages: 967-973
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Abstract

1-Methylpropyl 2-imidazolyl disulfide (PX-12) has been proposed as an inhibitor of thioredoxin-1 (Trx-1) with antitumor activity. However, the antitumor activity of the Trx-1 redox signaling inhibitor PX-12 on colorectal cancer is still obscure. In the present study, we showed that PX-12 inhibited the growth of colorectal cancer DLD-1 and SW620 cells in a dose- and time-dependent manner. Further analysis demonstrated that PX-12 reduced cell colony formation and induced a G2/M phase arrest of the cell cycle. In addition, PX-12 treatment induced apoptosis, as observed by the increased number of Annexin V-positive cells and increased activation of caspase-3. Notably, a low dose of PX-12 inhibited colorectal cancer cell migration and invasion. Treatment of cancer cells with PX-12 reduced NOX1, CDH17 and S100A4 mRNA expression, and increased KLF17 mRNA expression. Moreover, PX-12 decreased S100A4 protein expression in the colorectal cancer cells. Collectively, the present study demonstrates the antitumor effects and therapeutic potential of PX-12 in colorectal cancer.
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February-2015
Volume 33 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang F, Lin F, Zhang P, Ni W, Bi L, Wu J and Jiang L: Thioredoxin-1 inhibitor, 1-methylpropyl 2-imidazolyl disulfide, inhibits the growth, migration and invasion of colorectal cancer cell lines. Oncol Rep 33: 967-973, 2015
APA
Wang, F., Lin, F., Zhang, P., Ni, W., Bi, L., Wu, J., & Jiang, L. (2015). Thioredoxin-1 inhibitor, 1-methylpropyl 2-imidazolyl disulfide, inhibits the growth, migration and invasion of colorectal cancer cell lines. Oncology Reports, 33, 967-973. https://doi.org/10.3892/or.2014.3652
MLA
Wang, F., Lin, F., Zhang, P., Ni, W., Bi, L., Wu, J., Jiang, L."Thioredoxin-1 inhibitor, 1-methylpropyl 2-imidazolyl disulfide, inhibits the growth, migration and invasion of colorectal cancer cell lines". Oncology Reports 33.2 (2015): 967-973.
Chicago
Wang, F., Lin, F., Zhang, P., Ni, W., Bi, L., Wu, J., Jiang, L."Thioredoxin-1 inhibitor, 1-methylpropyl 2-imidazolyl disulfide, inhibits the growth, migration and invasion of colorectal cancer cell lines". Oncology Reports 33, no. 2 (2015): 967-973. https://doi.org/10.3892/or.2014.3652