Combination of HSV1-TK/shTERT by retrovirus vector inhibits hepatocellular carcinoma cell growth in vitro and in vivo

  • Authors:
    • Hongsheng Li
    • Qiaoyu Wang
    • Dongli Li
    • Quanshi Wang
    • Hubing Wu
  • View Affiliations

  • Published online on: December 23, 2014     https://doi.org/10.3892/or.2014.3697
  • Pages: 1307-1313
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Abstract

Hepatocellular carcinoma (HCC) is a common malignant carcinoma worldwide and the third leading cause of cancer mortality. However, current treatment strategies are not potent enough to combat this disease. Therefore, identification of novel and more effective treatments is crucial. Of the current methods, gene therapy, which targets cancer‑specific expression and limits toxicity, is a new strategy for treating cancers. In this study, we developed a retroviral vector containing herpes simplex virus type-1-thymidine kinase (HSV1‑TK) and a short hairpin RNA for the human telomerase reverse transcriptase (hTERT) gene and investigated the antitumor effects in an in vitro and in vivo mouse model of liver cancer, monitored by PET image. In vitro experiments on HCC cells in the TK-shTERT treatment group showed significant accumulation of 18F-FHBG, which preferentially inhibits HCC cell growth with extremely limited toxicity in normal cells. In vivo studies showed a significant reduction of growth in the TK-shTERT treatment group. In conclusion, these findings showed that combination HSV1-TK/hTERT gene therapy effectively and safely inhibits HCC cell growth in vitro and in vivo and is worthy of development in clinical trials for the treatment of HCC.
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March-2015
Volume 33 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Li H, Wang Q, Li D, Wang Q and Wu H: Combination of HSV1-TK/shTERT by retrovirus vector inhibits hepatocellular carcinoma cell growth in vitro and in vivo. Oncol Rep 33: 1307-1313, 2015
APA
Li, H., Wang, Q., Li, D., Wang, Q., & Wu, H. (2015). Combination of HSV1-TK/shTERT by retrovirus vector inhibits hepatocellular carcinoma cell growth in vitro and in vivo. Oncology Reports, 33, 1307-1313. https://doi.org/10.3892/or.2014.3697
MLA
Li, H., Wang, Q., Li, D., Wang, Q., Wu, H."Combination of HSV1-TK/shTERT by retrovirus vector inhibits hepatocellular carcinoma cell growth in vitro and in vivo". Oncology Reports 33.3 (2015): 1307-1313.
Chicago
Li, H., Wang, Q., Li, D., Wang, Q., Wu, H."Combination of HSV1-TK/shTERT by retrovirus vector inhibits hepatocellular carcinoma cell growth in vitro and in vivo". Oncology Reports 33, no. 3 (2015): 1307-1313. https://doi.org/10.3892/or.2014.3697