NRSF/REST regulates the mTOR signaling pathway in oral cancer cells

  • Authors:
    • Eugene Cho
    • Sung-Min Moon
    • Bo Ram Park
    • Do Kyung Kim
    • Byung-Kwon Lee
    • Chun Sung Kim
  • View Affiliations

  • Published online on: December 18, 2014     https://doi.org/10.3892/or.2014.3675
  • Pages: 1459-1464
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Abstract

The neuron-restrictive silencer factor/repressor element 1-silencing transcription factor (NRSF/REST) was originally discovered as a transcriptional repressor of neuronal genes in non-neuronal cells. However, it was recently reported to be abundantly expressed in several types of aggressive cancer cells, as well as in mature neurons. In the present study, the role of NRSF/REST in the human oral squamous cell carcinoma (SCC) KB cell line was evaluated. NRSF/REST was expressed at a higher level in KB cells when compared with that in normal human oral keratinocytes (NHOKs). Knockdown of NRSF/REST by siRNA reduced cell viability only in KB cells in a time-dependent manner, and this effect was due to the activation of apoptosis components and DNA fragmentation. In addition, knockdown of NRSF/REST disrupted the mTOR signaling pathway which is a key survival factor in many types of cancer cells. For example, the phosphorylation of elF4G, elF4E and 4E-BP1 was significantly reduced in the KΒ cells upon NRSF/REST knockdown. These results imply that NRSF/REST plays an important role in the survival of oral cancer cells by regulating the mTOR signaling pathway.
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March-2015
Volume 33 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Cho E, Moon S, Park BR, Kim DK, Lee B and Kim CS: NRSF/REST regulates the mTOR signaling pathway in oral cancer cells. Oncol Rep 33: 1459-1464, 2015
APA
Cho, E., Moon, S., Park, B.R., Kim, D.K., Lee, B., & Kim, C.S. (2015). NRSF/REST regulates the mTOR signaling pathway in oral cancer cells. Oncology Reports, 33, 1459-1464. https://doi.org/10.3892/or.2014.3675
MLA
Cho, E., Moon, S., Park, B. R., Kim, D. K., Lee, B., Kim, C. S."NRSF/REST regulates the mTOR signaling pathway in oral cancer cells". Oncology Reports 33.3 (2015): 1459-1464.
Chicago
Cho, E., Moon, S., Park, B. R., Kim, D. K., Lee, B., Kim, C. S."NRSF/REST regulates the mTOR signaling pathway in oral cancer cells". Oncology Reports 33, no. 3 (2015): 1459-1464. https://doi.org/10.3892/or.2014.3675