Induction and mechanism of HeLa cell apoptosis by 9-oxo‑10, 11-dehydroageraphorone from Eupatorium adenophorum

  • Authors:
    • Fei Liao
    • Yanchun Hu
    • Lei Wu
    • Hui Tan
    • Biao Luo
    • Yajun He
    • Yan Qiao
    • Quan Mo
    • Ya Wang
    • Zhicai Zuo
    • Junliang Deng
    • Yahui Wei
  • View Affiliations

  • Published online on: February 2, 2015     https://doi.org/10.3892/or.2015.3778
  • Pages: 1823-1827
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Abstract

9-Oxo-10, 11-dehydroageraphorone (euptox A), a cadenine sesquiterpene, is the main toxin from Eupatorium adenophorum. The aim of the present study was to examine the induction and mechanism of HeLa cell apoptosis by euptox A. The apoptosis‑inducing effect of the euptox A on HeLa cells was examined by MTT assay. The underlying mechanism was analyzed by flow cytometry and quantitative PCR. Flow cytometry results suggested that euptox A effectively inhibited the proliferation of HeLa cells, arrested the cell cycle transition from S to G2/M phase, did not continue to complete the cell cycle activity (mainly from 4 times and mitosis), and induced cell proliferation. The RT-qPCR detection results showed that euptox A induced apoptosis by improving the gene expression level of apoptotic proteases such as caspase-10 in HeLa cells. Its mechanism of action was associated with the upregulation of apoptotic gene expression and arresting of the cell cycle.

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April-2015
Volume 33 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Liao F, Hu Y, Wu L, Tan H, Luo B, He Y, Qiao Y, Mo Q, Wang Y, Zuo Z, Zuo Z, et al: Induction and mechanism of HeLa cell apoptosis by 9-oxo‑10, 11-dehydroageraphorone from Eupatorium adenophorum. Oncol Rep 33: 1823-1827, 2015
APA
Liao, F., Hu, Y., Wu, L., Tan, H., Luo, B., He, Y. ... Wei, Y. (2015). Induction and mechanism of HeLa cell apoptosis by 9-oxo‑10, 11-dehydroageraphorone from Eupatorium adenophorum. Oncology Reports, 33, 1823-1827. https://doi.org/10.3892/or.2015.3778
MLA
Liao, F., Hu, Y., Wu, L., Tan, H., Luo, B., He, Y., Qiao, Y., Mo, Q., Wang, Y., Zuo, Z., Deng, J., Wei, Y."Induction and mechanism of HeLa cell apoptosis by 9-oxo‑10, 11-dehydroageraphorone from Eupatorium adenophorum". Oncology Reports 33.4 (2015): 1823-1827.
Chicago
Liao, F., Hu, Y., Wu, L., Tan, H., Luo, B., He, Y., Qiao, Y., Mo, Q., Wang, Y., Zuo, Z., Deng, J., Wei, Y."Induction and mechanism of HeLa cell apoptosis by 9-oxo‑10, 11-dehydroageraphorone from Eupatorium adenophorum". Oncology Reports 33, no. 4 (2015): 1823-1827. https://doi.org/10.3892/or.2015.3778