Identification of potential targets for diallyl disulfide in human gastric cancer MGC-803 cells using proteomics approaches

  • Authors:
    • Bo Su
    • Jian Su
    • Hui He
    • Youhua Wu
    • Hong Xia
    • Xi Zeng
    • Wenxiang Dai
    • Xiaohong Ai
    • Hui Ling
    • Hao Jiang
    • Qi Su
  • View Affiliations

  • Published online on: March 17, 2015     https://doi.org/10.3892/or.2015.3859
  • Pages: 2484-2494
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Diallyl disulfide (DADS) is characterized as an effective agent for the prevention and therapy of cancer, however, mechanisms regarding its anticancer effects are not fully clarified. In the present study, we compared the protein expression profile of gastric cancer MGC-803 cells subjected to DADS treatment with that of untreated control cells to explore potential molecules regulated by DADS. Using proteomic approaches, we identified 23 proteins showing statistically significant differences in expression, including 9 upregulated and 14 downregulated proteins. RT-PCR and western blot analysis confirmed that retinoid-related orphan nuclear receptor α (RORα) and nM23 were increased by DADS, whereas LIM kinase-1 (LIMK1), urokinase-type plasminogen activator receptor (uPAR) and cyclin-dependent kinase-1 (CDK1) were decreased. DADS treatment and knockdown of uPAR caused suppression of ERK/Fra-1 pathway, downregulation of urokinase-type plasminogen activator (uPA), matrix metalloproteinase-9 (MMP-9) and vimentin, and upregulation of tissue inhibitor of metalloproteinase-3 (TIMP-3) and E-cadherin, concomitant with inhibition of cell migration and invasion. Moreover, knockdown of uPAR potentiated the effects of DADS on MGC-803 cells. These data demonstrate that downregulation of uPAR may partially be responsible for DADS-induced inhibition of ERK/Fra-1 pathway, as well as cell migration and invasion. Thus, the discovery of DADS‑induced differential expression proteins is conducive to reveal unknown mechanisms of DADS anti‑gastric cancer.
View Figures
View References

Related Articles

Journal Cover

May-2015
Volume 33 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Su B, Su J, He H, Wu Y, Xia H, Zeng X, Dai W, Ai X, Ling H, Jiang H, Jiang H, et al: Identification of potential targets for diallyl disulfide in human gastric cancer MGC-803 cells using proteomics approaches. Oncol Rep 33: 2484-2494, 2015
APA
Su, B., Su, J., He, H., Wu, Y., Xia, H., Zeng, X. ... Su, Q. (2015). Identification of potential targets for diallyl disulfide in human gastric cancer MGC-803 cells using proteomics approaches. Oncology Reports, 33, 2484-2494. https://doi.org/10.3892/or.2015.3859
MLA
Su, B., Su, J., He, H., Wu, Y., Xia, H., Zeng, X., Dai, W., Ai, X., Ling, H., Jiang, H., Su, Q."Identification of potential targets for diallyl disulfide in human gastric cancer MGC-803 cells using proteomics approaches". Oncology Reports 33.5 (2015): 2484-2494.
Chicago
Su, B., Su, J., He, H., Wu, Y., Xia, H., Zeng, X., Dai, W., Ai, X., Ling, H., Jiang, H., Su, Q."Identification of potential targets for diallyl disulfide in human gastric cancer MGC-803 cells using proteomics approaches". Oncology Reports 33, no. 5 (2015): 2484-2494. https://doi.org/10.3892/or.2015.3859