miR-154 suppresses non-small cell lung cancer growth in vitro and in vivo

Retraction in: /10.3892/or.2023.8558

  • Authors:
    • Xingyu Lin
    • Zhiguang Yang
    • Peng Zhang
    • Guoguang Shao
  • View Affiliations

  • Published online on: April 2, 2015     https://doi.org/10.3892/or.2015.3895
  • Pages: 3053-3060
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Abstract

miR-154 has been proven to act as a tumor suppressor in several types of tumors. However, its role in non-small cell lung cancer (NSCLC) remains unclear. Thus, the aim of this study was to investigate the effects of miR-154 on NSCLC tumorigenesis and development. Using real-time quantitative PCR (qRT-PCR), we analyzed expression of miR-154 at the transcriptional level in 40 NSCLC tumor tissues and matched adjacent normal tissues and the correlation with clinicopathological features of the patients. The miR-154 mimic was stably transfected into NSCLC A549 cells, and the effects of miR-154 on cancer cell proliferation, colony formation, cell cycle arrest, apoptosis, migration and invasion in vitro, and on the growth of in vivo xenografts were investigated. miR-154 expression levels were significantly downregulated in the NSCLC compared to the corresponding non-cancerous lung tissues (P<0.05), and decreased miR-154 expression was significantly associated with metastasis (P<0.001), larger tumor size (P<0.001) and advanced TNM stage (P<0.001). Furthermore, transfection of the miR-154 mimic into the NSCLC A549 cells was able to inhibit cell proliferation, colony formation, invasion and migration, and induce cell apoptosis and G0/G1 cell cycle arrest. Enforced expression of miR-154 also suppressed the growth of cancer cell xenografts in vivo. These findings indicate that miR-154 may become a potential target for miR-based therapy of NSCLC.
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June-2015
Volume 33 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lin X, Yang Z, Zhang P and Shao G: miR-154 suppresses non-small cell lung cancer growth in vitro and in vivo Retraction in /10.3892/or.2023.8558. Oncol Rep 33: 3053-3060, 2015
APA
Lin, X., Yang, Z., Zhang, P., & Shao, G. (2015). miR-154 suppresses non-small cell lung cancer growth in vitro and in vivo Retraction in /10.3892/or.2023.8558. Oncology Reports, 33, 3053-3060. https://doi.org/10.3892/or.2015.3895
MLA
Lin, X., Yang, Z., Zhang, P., Shao, G."miR-154 suppresses non-small cell lung cancer growth in vitro and in vivo Retraction in /10.3892/or.2023.8558". Oncology Reports 33.6 (2015): 3053-3060.
Chicago
Lin, X., Yang, Z., Zhang, P., Shao, G."miR-154 suppresses non-small cell lung cancer growth in vitro and in vivo Retraction in /10.3892/or.2023.8558". Oncology Reports 33, no. 6 (2015): 3053-3060. https://doi.org/10.3892/or.2015.3895