Aurora kinases are essential for PKC-induced invasion and matrix metalloproteinase-9 expression in MCF-7 breast cancer cells

  • Authors:
    • Eun-Mi Noh
    • Young-Rae Lee
    • On-Yu Hong
    • Sung Hoo Jung
    • Hyun Jo Youn
    • Jong-Suk Kim
  • View Affiliations

  • Published online on: June 4, 2015     https://doi.org/10.3892/or.2015.4027
  • Pages: 803-810
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The Aurora kinase family of serine/threonine kinases are known to be crucial for cell cycle control. Aurora kinases are considered a target of anticancer drugs. However, few studies have assessed the effect of Aurora kinases in breast cancer. In the present study, to determine whether Aurora kinases play a role in oncogenic actions of protein kinase C (PKC), we investigated the effect of Aurora kinases on PKC-induced invasion and MMP-9 expression using breast cancer cells. Treatment of MCF-7 cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) induced the upregulation and phosphorylation of Aurora kinases via the MAPK signaling pathway. Moreover, the inhibition of Aurora kinases by their siRNAs and inhibitors suppressed TPA-induced cell invasion and expression of MMP-9 by inhibiting the activation of NF-κB/AP-1, major transcription factors for MMP-9 expression in MCF-7 cells. These results suggested that Aurora kinases mediate PKC-MAPK signal to NF-κB/AP-1 with increasing MMP-9 expression and invasion of MCF-7 cells. To the best of our knowledge, this is the first study to show that Aurora kinases are key molecules in PKC-induced invasion in breast cancer cells.
View Figures
View References

Related Articles

Journal Cover

August-2015
Volume 34 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Noh E, Lee Y, Hong O, Jung SH, Youn HJ and Kim J: Aurora kinases are essential for PKC-induced invasion and matrix metalloproteinase-9 expression in MCF-7 breast cancer cells. Oncol Rep 34: 803-810, 2015
APA
Noh, E., Lee, Y., Hong, O., Jung, S.H., Youn, H.J., & Kim, J. (2015). Aurora kinases are essential for PKC-induced invasion and matrix metalloproteinase-9 expression in MCF-7 breast cancer cells. Oncology Reports, 34, 803-810. https://doi.org/10.3892/or.2015.4027
MLA
Noh, E., Lee, Y., Hong, O., Jung, S. H., Youn, H. J., Kim, J."Aurora kinases are essential for PKC-induced invasion and matrix metalloproteinase-9 expression in MCF-7 breast cancer cells". Oncology Reports 34.2 (2015): 803-810.
Chicago
Noh, E., Lee, Y., Hong, O., Jung, S. H., Youn, H. J., Kim, J."Aurora kinases are essential for PKC-induced invasion and matrix metalloproteinase-9 expression in MCF-7 breast cancer cells". Oncology Reports 34, no. 2 (2015): 803-810. https://doi.org/10.3892/or.2015.4027