MicroRNA-144 affects radiotherapy sensitivity by promoting proliferation, migration and invasion of breast cancer cells

  • Authors:
    • Lei Yu
    • Yanming Yang
    • Jiguang Hou
    • Chengwei Zhai
    • Yunhao Song
    • Zhiliang Zhang
    • Ling Qiu
    • Xiaojing Jia
  • View Affiliations

  • Published online on: August 5, 2015     https://doi.org/10.3892/or.2015.4173
  • Pages: 1845-1852
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Abstract

Radiotherapy resistance remains a major obstacle for patients with breast cancer. miRNAs are important regulators in many biological processes including proliferation, apoptosis, invasion and metastasis and response to treatment in different types of tumors. Here, we describe the role of miRNA-144 in the regulation of radiotherapy sensitivity, migration and invasion of breast cancer cells. The cell survival rate of breast cancer cells was measured by WST-1 assay after irradiation. The caspase-3/-7 activity and apoptotic proteins were analyzed by Caspase-Glo3/7 assay and western blot analysis, respectively. The migration and invasion of breast cancer cells were evaluated by BD Transwell migration and Matrigel invasion assays. The EMT markers were detected by western blot analysis. We found that overexpression of miR-144 increased the proliferation rate of MDA-MB-231 cells without radiation. Both MDA-MB‑231 and SKBR3 cells exhibited significantly increased radiation resistance after overexpression of miR-144. Meanwhile, the migration and invasion of both MDA-MB-231 and SKBR3 cells were changed by altered miR-144 expression. In addition, the overexpression of miR-144 inhibited E-cadherin expression and promoted Snail expression. miR-144 activated AKT by downregulation of PTEN in breast cancer cells. Our results strongly suggest that miR-144 acts as an important regulator of tumorigenesis and tumor progression of breast cancer. These results indicate that miR-144 might serve as a potential molecular target for breast cancer treatment.
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October-2015
Volume 34 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yu L, Yang Y, Hou J, Zhai C, Song Y, Zhang Z, Qiu L and Jia X: MicroRNA-144 affects radiotherapy sensitivity by promoting proliferation, migration and invasion of breast cancer cells. Oncol Rep 34: 1845-1852, 2015
APA
Yu, L., Yang, Y., Hou, J., Zhai, C., Song, Y., Zhang, Z. ... Jia, X. (2015). MicroRNA-144 affects radiotherapy sensitivity by promoting proliferation, migration and invasion of breast cancer cells. Oncology Reports, 34, 1845-1852. https://doi.org/10.3892/or.2015.4173
MLA
Yu, L., Yang, Y., Hou, J., Zhai, C., Song, Y., Zhang, Z., Qiu, L., Jia, X."MicroRNA-144 affects radiotherapy sensitivity by promoting proliferation, migration and invasion of breast cancer cells". Oncology Reports 34.4 (2015): 1845-1852.
Chicago
Yu, L., Yang, Y., Hou, J., Zhai, C., Song, Y., Zhang, Z., Qiu, L., Jia, X."MicroRNA-144 affects radiotherapy sensitivity by promoting proliferation, migration and invasion of breast cancer cells". Oncology Reports 34, no. 4 (2015): 1845-1852. https://doi.org/10.3892/or.2015.4173