miR-138 suppresses the proliferation of oral squamous cell carcinoma cells by targeting Yes-associated protein 1

  • Authors:
    • Ran Xu
    • Guang Zeng
    • Jing Gao
    • Yue Ren
    • Zhe Zhang
    • Qingna Zhang
    • Jinxiu Zhao
    • Hong Tao
    • Daxu Li
  • View Affiliations

  • Published online on: July 23, 2015     https://doi.org/10.3892/or.2015.4144
  • Pages: 2171-2178
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Abstract

Aberrant microRNA expression has been suggested to be an important event in the pathologies of various types of cancer. MicroRNA-138 (miR-138) has been reported to be frequently downregulated in various types of human cancer, including oral squamous cell carcinoma (OSCC). However, the precise molecular mechanism of miR-138 underlying OSCC remains largely unknown. The aim of the present study was to investigate the expression of miR-138 in OSCC tumor tissues and several OSCC cell lines and validated its interaction with the 3'-untranslated region (3'-UTR) of Yes-associated protein 1 (YAP1). The results showed that, miR-138 was significantly downregulated in OSCC tumor tissues and cell lines. Overexpression of miR-138 inhibited cell proliferation of OSCC cells whereas the downregulation of miR-138 promoted cell proliferation. A direct interaction between miR-138 and 3'-UTR of YAP1 was validated by dual-luciferase reporter assay. Moreover, overexpression of miR-138 in OSCC cells significantly decreased the expression of YAP1 and downregulation of miR-138 inhibited the expression of YAP1. Specifically, the inhibitory effect of miR-138 on the proliferation of OSCC cells was eliminated by transfection with YAP1 overexpression vectors that did not harbor any specific miR-138 binding specific sequences in 3'-UTR. In addition, the miR-138‑overexpressing OSCC cells exhibited a low growth rate in the xenograft tumor assay with a decreased expression of YAP1 in tumor tissues. The results suggest that miR-138 is a tumor suppressor miRNA in OSCC through targeting YAP1, which serves as a promising therapeutic target for the treatment of OSCC.
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October-2015
Volume 34 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xu R, Zeng G, Gao J, Ren Y, Zhang Z, Zhang Q, Zhao J, Tao H and Li D: miR-138 suppresses the proliferation of oral squamous cell carcinoma cells by targeting Yes-associated protein 1. Oncol Rep 34: 2171-2178, 2015
APA
Xu, R., Zeng, G., Gao, J., Ren, Y., Zhang, Z., Zhang, Q. ... Li, D. (2015). miR-138 suppresses the proliferation of oral squamous cell carcinoma cells by targeting Yes-associated protein 1. Oncology Reports, 34, 2171-2178. https://doi.org/10.3892/or.2015.4144
MLA
Xu, R., Zeng, G., Gao, J., Ren, Y., Zhang, Z., Zhang, Q., Zhao, J., Tao, H., Li, D."miR-138 suppresses the proliferation of oral squamous cell carcinoma cells by targeting Yes-associated protein 1". Oncology Reports 34.4 (2015): 2171-2178.
Chicago
Xu, R., Zeng, G., Gao, J., Ren, Y., Zhang, Z., Zhang, Q., Zhao, J., Tao, H., Li, D."miR-138 suppresses the proliferation of oral squamous cell carcinoma cells by targeting Yes-associated protein 1". Oncology Reports 34, no. 4 (2015): 2171-2178. https://doi.org/10.3892/or.2015.4144