HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation

  • Authors:
    • Jiliang Yan
    • Chunyan Ma
    • Jian Cheng
    • Zhengguo Li
    • Chao Liu
  • View Affiliations

  • Published online on: August 13, 2015     https://doi.org/10.3892/or.2015.4202
  • Pages: 2776-2781
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Abstract

HS1 associated protein X-1 (HAX-1), a substrate of Src family tyrosine kinases, plays a critical role in cell apoptosis. However, its functions in prostate cancer remains unclear. The present study explored the role and mechanism of HAX-1 in cancer cell apoptosis. The mRNA and protein levels of HAX-1 in the prostate cancer cell lines PC-3, VCaP and DU145 were assessed. Cell proliferation, apoptosis and caspase-9 activities were assessed in DU145 after HAX-1 siRNA treatment. The mRNA and protein levels of HAX-1 in prostate cancer cell lines PC-3, VCaP and DU145 were significantly higher than those in the primary prostate epithelial cells, and DU145 possess the highest mRNA and protein levels compared to PC-3 and VCaP. When HAX-1 was knocked down in DU145, cell proliferation was significantly decreased, accompanied by a decrease in Ki67 protein expression. Compared with the control and control siRNA groups, HAX-1 siRNA promoted cell apoptosis and caspase-9 activation in DU145. Furthermore, prostate cancer cells co-transfected with HAX-1 and caspase-9 promoted viability and reduced apoptosis. In contract, co-transfection of caspase-9 and HAX-1 siRNA suppressed the cell viability and enhanced apoptosis. In summary, the present study demonstrated that HAX-1 inhibits cell apoptosis through caspase-9 inactivation.
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November-2015
Volume 34 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yan J, Ma C, Cheng J, Li Z and Liu C: HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation. Oncol Rep 34: 2776-2781, 2015
APA
Yan, J., Ma, C., Cheng, J., Li, Z., & Liu, C. (2015). HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation. Oncology Reports, 34, 2776-2781. https://doi.org/10.3892/or.2015.4202
MLA
Yan, J., Ma, C., Cheng, J., Li, Z., Liu, C."HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation". Oncology Reports 34.5 (2015): 2776-2781.
Chicago
Yan, J., Ma, C., Cheng, J., Li, Z., Liu, C."HAX-1 inhibits apoptosis in prostate cancer through the suppression of caspase-9 activation". Oncology Reports 34, no. 5 (2015): 2776-2781. https://doi.org/10.3892/or.2015.4202