Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability

  • Authors:
    • Hyun-Woo Kim
    • Soo -A Kim
    • Sang-Gun Ahn
  • View Affiliations

  • Published online on: November 2, 2015     https://doi.org/10.3892/or.2015.4381
  • Pages: 235-242
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Abstract

The histone deacetylases (HDACs), Sirtuin 1 (Sirt1) and Sirt2, play crucial roles in many biological processes, including cell proliferation, differentiation and apoptosis. HDAC inhibitors have been considered as a potential therapeutic approach for various types of cancers. Here, we demonstrated that the Sirt1 and Sirt2 inhibitors EX527 and AGK2 suppressed cell growth and caused G1 phase arrest by inhibiting the expression of Cdk6 and/or Cdk4. An agar colony formation assay revealed that EX527 and AGK2 decreased colony formation in soft agar. Furthermore, EX527 and AGK2 pretreatment inhibited the expression of HSF1 and HSP27 and induced HSF1 ubiquitination. Sirt1 overexpression increased HSF1 expression and/or stabilization and induced cell migration in a scratch assay. Overall, these results indicate that EX527 and AGK2 suppress cell growth and migration by inhibiting HSF1 protein stability.
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January-2016
Volume 35 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kim H, Kim S and Ahn S: Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability. Oncol Rep 35: 235-242, 2016
APA
Kim, H., Kim, S., & Ahn, S. (2016). Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability. Oncology Reports, 35, 235-242. https://doi.org/10.3892/or.2015.4381
MLA
Kim, H., Kim, S., Ahn, S."Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability". Oncology Reports 35.1 (2016): 235-242.
Chicago
Kim, H., Kim, S., Ahn, S."Sirtuin inhibitors, EX527 and AGK2, suppress cell migration by inhibiting HSF1 protein stability". Oncology Reports 35, no. 1 (2016): 235-242. https://doi.org/10.3892/or.2015.4381