Open Access

Depletion of Cks1 and Cks2 expression compromises cell proliferation and enhance chemotherapy-induced apoptosis in HepG2 cells

  • Authors:
    • Lingqing Lin
    • Zanxi Fang
    • Huayue Lin
    • Hanyu You
    • Jiajia Wang
    • Yuanhui Su
    • Fen Wang
    • Zhong‑Ying Zhang
  • View Affiliations

  • Published online on: November 2, 2015     https://doi.org/10.3892/or.2015.4372
  • Pages: 26-32
  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study explored the oncogenic roles of overexpressed Cks1 and Cks2 in human hepatocellular carcinoma cells. Gene expression of Cks1 and Cks2 in HepG2 cells was disrupted by siRNA or increased by cDNA transfection. Cell proliferation was assayed by CCK-8 analysis and cell counting. Cisplatin-induced apoptosis after transfection was measured by flow cytometry using Annexin V/propidium iodide (PI) double staining. Cell cycle changes after transfection were determined by flow cytometry with PI staining. Protein levels of Akt and GSK-3β were measured after transfection. The results revealed that HepG2 proliferation was decreased by depletion of endogenous Cks1 or Cks2, and increased by overexpression of Cks1 or Cks2. HepG2 apoptosis increased concordantly with the decline of Cks1 or Cks2 expression. Overexpression of Cks1 or Cks2 prevented cell apoptosis. Protein levels of p‑Akt and p‑GSK-3β were downregulated after RNA interference of Cks1 or Cks2. In conclusion, Cks1 and Cks2 promoted proliferation and prevented apoptosis of HepG2 cells. The Akt/GSK-3β-related PI3K/Akt signaling pathway may be a key signaling pathway that is involved in the regulation of cell growth and cell death.
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January-2016
Volume 35 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lin L, Fang Z, Lin H, You H, Wang J, Su Y, Wang F and Zhang ZY: Depletion of Cks1 and Cks2 expression compromises cell proliferation and enhance chemotherapy-induced apoptosis in HepG2 cells. Oncol Rep 35: 26-32, 2016
APA
Lin, L., Fang, Z., Lin, H., You, H., Wang, J., Su, Y. ... Zhang, Z. (2016). Depletion of Cks1 and Cks2 expression compromises cell proliferation and enhance chemotherapy-induced apoptosis in HepG2 cells. Oncology Reports, 35, 26-32. https://doi.org/10.3892/or.2015.4372
MLA
Lin, L., Fang, Z., Lin, H., You, H., Wang, J., Su, Y., Wang, F., Zhang, Z."Depletion of Cks1 and Cks2 expression compromises cell proliferation and enhance chemotherapy-induced apoptosis in HepG2 cells". Oncology Reports 35.1 (2016): 26-32.
Chicago
Lin, L., Fang, Z., Lin, H., You, H., Wang, J., Su, Y., Wang, F., Zhang, Z."Depletion of Cks1 and Cks2 expression compromises cell proliferation and enhance chemotherapy-induced apoptosis in HepG2 cells". Oncology Reports 35, no. 1 (2016): 26-32. https://doi.org/10.3892/or.2015.4372