CD24 promotes the proliferation and inhibits the apoptosis of cervical cancer cells in vitro

  • Authors:
    • Zhen Pei
    • Guangchao Zhu
    • Xiaolei Huo
    • Lu Gao
    • Shan Liao
    • Junyu He
    • Yuehua Long
    • Hong Yi
    • Songshu Xiao
    • Wei Yi
    • Pan Chen
    • Xiaoling Li
    • Guiyuan Li
    • Yanhong Zhou
  • View Affiliations

  • Published online on: December 24, 2015     https://doi.org/10.3892/or.2015.4521
  • Pages: 1593-1601
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Abstract

The protein CD24 is a cell surface protein that appears to function as an adhesion molecule; its expression has been shown to correlate with prognosis in a variety of tumors. Herein, we investigated the possible role and mechanism of CD24 in cervical cancer. Our results showed that CD24 was overexpressed in cervical cancer tissues compared with that in the adjacent non‑cancerous tissues by qPCR, immunohistochemistry and western blotting technologies. To explore the possible mechanism of CD24 in cervical cancer, we elucidated the effect of CD24 on the proliferation and apoptosis of cervical cancer HeLa cells and found that a considerable increase in cell proliferation was observed in the HeLa cells with CD24 overexpession. The rate of cell apoptosis was decreased in the HeLa/CD24 cells compared with the HeLa or HeLa/vector cells. Cell apoptosis is closely related with a reduction in mitochondrial membrane potential (ΔΨm) and an increase in intracellular reactive oxygen species (ROS) and calcium ion (Ca2+) concentrations. Our results showed that overexpression of CD24 in the cervical cancer HeLa cells, led to an increase in ΔΨm and a decrease in intracellular ROS and Ca2+ concentrations. Furthermore, we found that CD24 was correlated with dysregulation of the MAPK signaling pathway in cervical cancer tissues in vitro. At the same time, we found that CD24 overexpression affected the expression of p38, JNK2 and c-Jun in vitro. In summary, our results suggest that CD24 is upregulated in cervical cancer tissues and plays its functions by affecting the MAPK signaling pathway in cervical cancer.
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March-2016
Volume 35 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Pei Z, Zhu G, Huo X, Gao L, Liao S, He J, Long Y, Yi H, Xiao S, Yi W, Yi W, et al: CD24 promotes the proliferation and inhibits the apoptosis of cervical cancer cells in vitro. Oncol Rep 35: 1593-1601, 2016
APA
Pei, Z., Zhu, G., Huo, X., Gao, L., Liao, S., He, J. ... Zhou, Y. (2016). CD24 promotes the proliferation and inhibits the apoptosis of cervical cancer cells in vitro. Oncology Reports, 35, 1593-1601. https://doi.org/10.3892/or.2015.4521
MLA
Pei, Z., Zhu, G., Huo, X., Gao, L., Liao, S., He, J., Long, Y., Yi, H., Xiao, S., Yi, W., Chen, P., Li, X., Li, G., Zhou, Y."CD24 promotes the proliferation and inhibits the apoptosis of cervical cancer cells in vitro". Oncology Reports 35.3 (2016): 1593-1601.
Chicago
Pei, Z., Zhu, G., Huo, X., Gao, L., Liao, S., He, J., Long, Y., Yi, H., Xiao, S., Yi, W., Chen, P., Li, X., Li, G., Zhou, Y."CD24 promotes the proliferation and inhibits the apoptosis of cervical cancer cells in vitro". Oncology Reports 35, no. 3 (2016): 1593-1601. https://doi.org/10.3892/or.2015.4521