PRE-CONGRESS WORKSHOP
Digital PCR - Applications and analysis (theoretical)

4-5 October 2016
Metropolitan Hotel
Athens, Greece

Deadline for registrations: 30 September 2016


WORKSHOP

Title: Digital PCR - Applications and analysis (theoretical)


Description: This course gives an overview of digital PCR and its current applications. We discuss the various strategies to measure dPCR including their advantages and limitations. We also go through the design of dPCR experiments including the challenges to obtain reliable results and the analysis of the dPCR data. Finally, we look into the future discussing forthcoming dPCR applications.

The course content provides information on:


  • When is digital PCR useful?
  • How to use digital PCR for absolute quantification
  • The advantage of digital PCR for rare mutation detection
  • How to do linkage analysis with digital PCR
  • How to use digital PCR for analysis of Next generation sequencing libraries
  • Gene expression analysis with digital PCR
  • What is Poisson distribution?
  • What is the error in our measurement?
  • What is it that affects the precision?
  • What is it that affects the accuracy?
  • Which controls are needed?
  • Commercially available instruments
  • How do they work?
  • Which are their differences (advantages/limitations)?
  • What to consider when designing assays for digital PCR
  • Design and optimization of digital PCR experiments
  • Trouble shooting
  • What is specific in the digital MIQE guidelines?
  • Which information is important from a digital PCR experiment?

The course is held by TATAA Biocenter, that has been a qPCR course provider since 2001 and have trained thousands of satisfied customers. We want to share our knowledge with you too!

Course leader: Dr David Svec, senior researcher, TATAA Biocenter

David Svec received his PhD in molecular biology at Charles University in Prague in 2014. During his PhD he worked in academic group in Prague (qPCR tomography, SPIDIA, laser microdissection, high-throughput qPCR) and also in TATAA Biocenter in Gothenburg, Sweden (spikes, ValidPrime, IPC, CTC panel). After his PhD, David did his post-doc with Anders Stahlberg at Cancer research center at Gothenburg University and Sahlgrenska hospital. He has held courses in TATAA´s regime since 2007 and currently is involved in product development, single cell analysis and consulting. David takes part also in few projects in basic research (single cell picking, digital PCR, multianalyte profiling).

Place: Metropolitan Hotel, Athens, Greece
Date4-5th of October 2016
Time: 9:00 a.m. - 17:00 p.m.

Registration fees: 590 EUR (Academic delegates),  690 EUR (Industrial delegates)

For questions or any further requests please send an email to workshops@spandidos-publications.com.