Open Access

LncRNA Meg3 promotes oxygen and glucose deprivation injury by decreasing angiogenesis in hBMECs by targeting the miR‑122‑5p/NDRG3 axis

  • Authors:
    • Zhaoliang Luo
    • Tingliang Gong
    • Weihong Li
    • Wenqiang Tao
  • View Affiliations

  • Published online on: August 11, 2022     https://doi.org/10.3892/etm.2022.11559
  • Article Number: 622
  • Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oxygen‑glucose deprivation (OGD) is widely used as an in vitro model for stroke. The present study aimed to explore the mechanisms of action of long non‑coding RNA (lncRNA) maternally expressed gene 3 (Meg3) in angiogenesis following OGD. The human brain microvascular endothelial cell line, hCMEC/D3, was used to establish the OGD model. lncRNA Meg3 was highly expressed in hCMEC/D3 cells subjected to OGD. Furthermore, it was found that the overexpression of lncRNA Meg3 decreased the proliferation, migration and angiogenesis of hCMEC/D3 cells subjected to OGD, and increased cell apoptosis. Meg3 silencing exerted the opposite effects. Subsequently, lncRNA Meg3 increased the expression of NDRG family member 3 (NDRG3) by directly binding to miR‑122‑5p. The overexpression of miR‑122‑5p and the knockdown of NDRG3 reversed the inhibitory effects of Meg3 overexpression on the proliferation, migration and angiogenesis of hCMEC/D3 cells subjected to OGD, as well as the promoting effects of Meg3 overexpression on cell apoptosis. The present study demonstrated that lncRNA Meg3 functions as a competing endogenous RNA by targeting the miR‑122‑5p/NDRG3 axis in regulating OGD injury.

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October-2022
Volume 24 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Luo Z, Gong T, Li W and Tao W: LncRNA Meg3 promotes oxygen and glucose deprivation injury by decreasing angiogenesis in hBMECs by targeting the miR‑122‑5p/NDRG3 axis. Exp Ther Med 24: 622, 2022
APA
Luo, Z., Gong, T., Li, W., & Tao, W. (2022). LncRNA Meg3 promotes oxygen and glucose deprivation injury by decreasing angiogenesis in hBMECs by targeting the miR‑122‑5p/NDRG3 axis. Experimental and Therapeutic Medicine, 24, 622. https://doi.org/10.3892/etm.2022.11559
MLA
Luo, Z., Gong, T., Li, W., Tao, W."LncRNA Meg3 promotes oxygen and glucose deprivation injury by decreasing angiogenesis in hBMECs by targeting the miR‑122‑5p/NDRG3 axis". Experimental and Therapeutic Medicine 24.4 (2022): 622.
Chicago
Luo, Z., Gong, T., Li, W., Tao, W."LncRNA Meg3 promotes oxygen and glucose deprivation injury by decreasing angiogenesis in hBMECs by targeting the miR‑122‑5p/NDRG3 axis". Experimental and Therapeutic Medicine 24, no. 4 (2022): 622. https://doi.org/10.3892/etm.2022.11559