Open Access

Xanthatin inhibits corneal neovascularization by inhibiting the VEGFR2‑mediated STAT3/PI3K/Akt signaling pathway

  • Authors:
    • Mei Shen
    • Xue‑Zhi Zhou
    • Lei Ye
    • Qing Yuan
    • Ce Shi
    • Pei‑Wen Zhu
    • Nan Jiang
    • Ming‑Yang Ma
    • Qi‑Chen Yang
    • Yi Shao
  • View Affiliations

  • Published online on: April 30, 2018     https://doi.org/10.3892/ijmm.2018.3646
  • Pages: 769-778
  • Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Alkali burn is one of the main causes of corneal injury. The inflammation and neovascularization caused by alkali burns aggravate corneal damage, resulting in loss of vision. The aim of the present study was to evaluate the efficacy of xanthatin in the treatment of alkali burn‑induced inflammation and neovascularization. A CCK‑8 assay was used to detect the effects of different concentrations of xanthatin on the proliferation of human umbilical vein endothelial cells (HUVECs). The effects of xanthatin on the migration of HUVECs and the ability of lumen formation were examined using a scratch test and lumen formation assay, respectively. A total of 60 Sprague‑Dawley rats were randomly divided into two groups to establish a corneal alkali burn model, and were treated with PBS and xanthatin eye drops four times a day. A slit lamp microscope recorded changes of the cornea at 0, 4, 7, 10 and 14 days, and the inflammatory indices of the cornea and the neovascular area were evaluated. The expression levels of vascular endothelial growth factor (VEGF) and pigment epithelium‑derived factor (PEDF) in the cornea under different treatment conditions were detected using immunofluorescence and western blot analysis. In order to investigate the mechanism of xanthatin on the inhibition of inflammation and neovascularization, HUVECs were treated with xanthatin and PBS following VEGF treatment. The subcellular localization of signal transducer and activator of transcription 3 (STAT3) was detected using immunofluorescence. The expression levels of VEGF receptor 2 (VEGFR2), STAT3, phosphoinositide 3‑kinase (PI3K) and Akt were detected using western blot analysis. The results revealed that xanthatin inhibited the proliferation of HUVECs in a concentration‑dependent manner. The migration ability and lumen‑forming ability of the HUVECs were also inhibited by xanthatin. Slit lamp microscopy showed that the inflammatory index and the area of neovascularization in the xanthatin‑treated group were significantly reduced, compared with those in the PBS treatment group. The xanthatin treatment group exhibited a lower protein expression level of VEGF and increased protein expression level of PEDF, compared with the PBS treatment group. In the VEGF‑treated HUVECs, xanthatin significantly decreased the expression levels of p‑VEGFR2, phosphorylated (p‑)STAT3, p‑PI3K and p‑Akt. In conclusion, the present study confirmed that xanthatin inhibited corneal neovascularization and inflammation in the alkali burn model, elucidating the underlying mechanisms involved in its protective effects. Therefore, xanthatin may be a novel drug for the treatment of corneal alkali burn.

Introduction

As an important feature of the anterior corneal refractive component, normal corneal function is indispensable for the formation of visual function and protection of the eye. Under hypoxia, inflammation, trauma and other harmful conditions, inflammatory cells secrete a variety of angiogenic chemokines, including basic fibroblast growth factor, vascular endothelial growth factor (VEGF) and prostaglandin, which lead to endothelial cell proliferation, differentiation, migration and chemotaxis. This promotes angiogenesis invasion from the corneal limb vascular network, which affects corneal transparency and eventually leads to blindness (1). According to the World Health Organization statistics, corneal disease is currently the second leading cause of disease-related blindness, whereas keratitis and neovascularization are the major causes of corneal damage-induced blindness. Current clinical studies are mainly focused on the identification of novel anti-inflammatory and anti-angiogenic drugs (2).

There are ~25 Xanthium species in the world (3). At present, xanthatin is the main medicinal component of Xanthium in China, which is used for the treatment of cold headaches, nasal mucus, itching, rhinorrhea and muscular constriction (4). Sesquiterpene lactone is a biologically active component of medicinal plants. The sesquiterpene lactone components of Xanthium sibiricum often contain α,β unsaturated carbonyl y-lactone-y fragments, which have beneficial biological activities, including antimicrobial, antioxidant, anti-allergy, anti-inflammatory, hypoglycemic and other pharmacological effects (2). Xanthatin is the main active constituent of sesquiterpene lactones in Xanthium sibiricum. It has antitumor (5), antifungal (6) and antiplasmodium (7) activities, and has attracted substantial attention in widespread studies.

Neovascularization is the process of vascular instability, proliferation and migration of vascular endothelial cells. At present, there are at least six different associated growth factors involved in the process of neovascularization, in which VEGF is the most important angiogenic factor. The effect of VEGF on vascular endothelial cells is predominantly regulated by two types of tyrosine kinase receptors, VEGF receptor (VEGFR)1 and VEGFR2 (8,9). VEGFR2 is more important in regulating the mitosis and penetration of endothelial cells (10,11). Xanthatin can effectively inhibit the VEGFR2 signaling pathway, thereby inhibiting angiogenesis and reducing inflammation (12). In addition, xanthatin can exert anti-inflammatory effects by inhibiting the synthesis of proinflammatory substances nitric oxide, tumor necrosis factor-α and prostaglandin E2, and the activity of 5-lipoxygenase (13).

Corneal alkali burn is a model of a severe ocular surface disease, which can lead to corneal ulcers, severe keratitis, corneal neovascularization and corneal scarring. Therefore, it is widely used to investigate the mechanism and treatment of corneal inflammation and angiogenesis. In the present study, the application of xanthatin eye drops was used for the treatment of corneal alkali burn, and the effects of xanthatin on inflammation and neovascularization were evaluated. The experimental results showed that xanthatin inhibited corneal neovascularization, reduced inflammation and assisted in repair of the corneal epithelium. Therefore, xanthatin is expected to be an effective drug for the treatment of keratitis and neovascularization.

Materials and methods

Cell culture

Human umbilical vein endothelial cells (HUVECs) which were obtained from the Cell Line Bank of the Chinese Academy of Sciences (Shanghai, China) were cultured with endothelial cell basal medium 2 (ECM2) containing nonessential amino acids, vitamins, growth factor, 2% FCS (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1% penicillin/streptomycin at 37°C in an atmosphere containing 5% CO2.

CCK-8 assay for cell proliferation

The passage 3–6 adherent HUVECs were washed with PBS and digested into cell suspension with 0.25% trypsin (EDTA) cells, and seeded in 96-well plates (5×103 cells/well) in eight groups. The cells were cultured under 5% CO2 in 37°C for 24 h, and 100-µl volumes of different concentrations of xanthatin in ECM2 were added. The final concentrations of xanthatin were 0.1, 0.5, 1, 2, 5, 10 and 20 µM. The cells were washed once with PBS following culture for 72 h, and were then incubated with serum-free ECM2 with 10 µl CCK-8 solution for 1 h. The OD 450 nm absorbance was measured using a microplate spectrophotometer.

Scratch assay for cell migration

When the cells (1×105/well) were grown to almost confluence, the culture medium in 24-well plates was replaced with serum-free medium for 24 h and cells were divided into two groups: A control group and a 10 µM xanthatin treatment group. A sterile scratch was made with a 200-µl tip to establish a cell wound model. The width of the wound was measured under phase contrast microscopy. Images were captured at 6 and 12 h post-scratching, and the experiment was repeated three times.

Tube formation test

A 150-µl 1:1 Matrigel and medium mixture was added into a pre-chilled 24-well plate, and incubated at 37°C for 1 h. The cells (2×104) treated with control or xanthatin were seeded into the Matrigel-coated 24-well plate and cultured for 6 h. Lumen formation was observed under an inverted microscope. The cells were fixed in 4% PFA, following which images were captured and the lumen lengths were quantified using ImageJ software version 2X (National Institutes of Health, Bethesda, MD, USA).

Establishment and treatment of the alkali burn model

A total of 60 male adult Sprague-Dawley rats weighing ~180–220 g were purchased from the Shilaike Experimental Animal Center (Shanghai, China). Following general anesthesia with ether inhalation, all rats were examined for neovascularization, inflammatory response and other lesions. All the rats were administered in the right eye with 1% tetracaine drops every 2 min for three times for corneal surface anesthesia. A single layer circular filter paper (3 mm diameter) was immersed in 1 mol/l sodium hydroxide solution for 20 sec, and the filter paper was accurately affixed to the central corneal surface of the right eye for 20 sec following the absorbing of excess liquid. The filter paper was then removed from the experimental eye and immediately washed with saline for 1 min. The rats were randomly divided into two groups: A control group and an experimental group, with 30 rats in each group. Following the induction of injury, the control group was treated with PBS (10 µl each time, four times per day). In the experimental group, xanthatin solution dissolved in PBS, with a final concentration of 10 µM, was applied (10 µl each time, four times per day). The course of treatment was 14 days. The corneas were observed at 0, 4, 7, 10 and 14 days, and the corneal neovascularization was recorded using a slit lamp to verify the damage of the corneal epithelium. The rats were sacrificed on day 14 of treatment, and the corneas were placed in a freezer (−80°C) for later experiments. Ethics approval was obtained from the Medical Ethics Committee of The First Affiliated Hospital of Nanchang University (Nanchang, China).

Measurement of corneal neovascularization

The rat corneas were observed with a slit lamp under a microscope at 4, 7, 10 and 14 days, and the area of corneal neovascularization was measured based on the vertical length of the longest vessel observed to grow continuously toward the center of the corneal opacity. The formula of corneal neovascularization was calculated according to the Robert model: S = C/12 × 3.1416 × [r2 − (r − I)2], where C is the time of the circumference of the cornea that accumulates for the new blood vessel (14), r is the corneal radius, and L is the length of corneal neovascularization from the corneal limbus into the cornea.

Histological examination

The corneas were harvested at the end of the experiment, cut into small pieces and fixed in 4% PFA. The samples were processed via routine dehydration with ethanol gradient, clearance with xylene, and then embedded in paraffin. The coronal section was cut into 5-µm thick tissue sections parched on the slide. The slides were deparaffinized, stained with hematoxylin and eosin, dehydrated with alcohol, and mounted in neutral balsam by using an Eclipse 50i clinical microscope (Nikon Corporation, Tokyo, Japan).

Western blot analysis

The corneal tissues of the rats were removed on day 14 post-alkali burn, ground in 100 µl lysis buffer for 20 min, and homogenized on ice three times (30 sec each with intervals of 20 sec). The homogenate was incubated on ice for 20 min and centrifuged at 4°C at 2,000 × g for 20 min. Following determination of the protein concentration of the sample using BCA standards, and sampling buffer was added to the protein sample and then boiled for denaturalization. The protein samples of each group were analyzed on a 10% SDS-PAGE gel and transferred onto a PVDF membrane following electrophoresis. Following blocking in 2% BSA (Sigma-Aldrich; F Merck KGaA, Darmstadt, Germany) for 1 h at room temperature, the membrane was incubated with VEGF (cat. no. ab46154; 1:200; Abcam, Cambridge, MA, USA), pigment epithelium-derived factor (PEDF) (cat. no. sc-25594; 1:200; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), phos-phorylated (p-)VEGFR2 (cat. no. ab5473; 1:500; Abcam), total (t)VEGFR2 (cat. no. ab10972; 1:500; Abcam), p-signal transducer and activator of transcription 3 (STAT3) (cat. no. ab76315; 1:2,000; Abcam), t-STAT3 (cat. no. ab68153; 1:1,000; Abcam), p-phosphoinositide 3-kinase (PI3K) (cat. no. 4228s; 1:1,000; Cell signaling Technology, Inc., Danvers, MA, USA), t-PI3K (cat. no. ab86714; 1:1,000; Abcam, Cambridge, MA, USA), p-Akt (cat. no. 4060; 1:1,000; Cell signaling Technology, Inc.), t-Akt (cat. no. 4685; 1:1,000; Cell signaling Technology, Inc.) and β-actin (cat. no. A5316; 1:10,000; Sigma-Aldrich; Merck KGaA) antibodies overnight at 4°C. Once the incubation was complete, the membrane was washed with TBST at room temperature three times on a rocking device (10 min each time) Following incubation for 2 h with secondary horseradish peroxidase antibody (Goat anti-Rabbit IgG HRP Affinity; cat. no. HAF008; 1:5,000; R&D Systems, Inc., Minneapolis, MN, USA) at room temperature, the membrane was washed with TBST three times at room temperature (10 min each time). Finally, the ECL color solution was added onto the membrane in the darkroom to develop the film. Images were analyzed using ImageJ software to calculate the gray values.

Immunofluorescence detection

Following deparaffinization, the slides were placed in EDTA buffer and boiled in a microwave for antigen retrieval. Following cooling, the slides were washed with PBS three times (5 min each). The slides were then blocked with 5% BSA for 20 min, and incubated with 50 µl diluted primary antibodies against VEGF (cat. no. ab46154; 1:200; Abcam) and PEDF (cat. no. sc-25594; 1:200; Santa Cruz Biotechnology, Inc.) at 4°C overnight. Following washing with PBS, 50 µl of diluted secondary antibody (goat anti-rabbit IgG; cat. no. F-6005; Lot:065k6224; 1:100, Sigma-Aldrich; Merck KGaA) was added to each slide, incubated at room temperature for 1 h and counterstained with DAPI. The slides were mounted with anti-quenching media and were maintained at 4°C in the dark until images were captured using the Leica upright microscope (DM2500; Leica Microsystems GmbH, Wetzlar, Germany).

Statistical analysis

The experimental data were analyzed using SPSS 20.0 (IBM SPSS, Armonk, NY, USA) and all experimental data are expressed as the mean ± standard deviation. Comparisons between groups were assessed using a post hoc Tukey test and correlation analysis. P<0.05 was considered to indicate a statistically significant difference.

Results

Effect of xanthatin on the cell proliferation capacity of HUVECs

To investigate the mechanism of xanthatin in corneal neovascularization, the present study investigated the effects of different concentrations of xanthatin on the proliferation of cultured HUVECs. The CCK-8 experimental results showed no significant inhibition of cell proliferation in the 0.1 µM xanthatin group, whereas 0.5, 1, 2, 5, 10 and 20 µM xanthatin reduced the HUVEC proliferation rate significantly (P<0.05; Fig. 1A). This indicated that different concentrations of xanthatin significantly affected the proliferation of HUVECs.

The results also showed that the effects of 10 and 20 µM xanthatin on the proliferation of HUVECs were equivalent, indicating that 10 µM may be the optimal concentration for inhibiting the proliferation of HUVECs.

Effect of xanthatin on the migration of HUVECs

In order to further investigate the association between xanthatin and HUVEC migration ability, a scratch test was performed to detect HUVEC migration in a control group and a 10 µM xanthatin treatment group (Fig. 1B). Images of the cells in each group were captured under an optical microscope, and each image was captured in the same region close to the scratch. After 0, 6 and 12 h, the gap widths between the two groups were significantly different, suggesting that 10 µM xanthatin had a marked effect on inhibiting the HUVEC migration ability (Fig. 1C).

Effect of xanthatin on luminal formation of HUVECs

The in vitro tube forming assay showed that the HUVECs formed several densely arranged luminal structures in each image of the control group, whereas fewer luminal structures were visible in the 10 µM xanthatin group, which were arranged more sparsely (Fig. 1D). Statistical analysis was performed on the length of the lumen. The results showed that, compared with the control group, the length of the lumen in the 10 µM xanthatin treatment group was ~50% of the length of lumen in the control group (Fig. 1E). Compared with the control group, treatment with 10 µM xanthatin significantly inhibited the formation of HUVEC lumen length (P<0.05).

Analysis of the physiological condition of the alkali burn animal model

Following the establishment of the alkali burn model, the rats were randomly divided into two groups, with 30 rats in each group. One group of rats was administered with PBS eye drops four times a day; the other group was administered with 10 µM xanthatin eye drops four times a day (Fig. 2A). The body weights and eye weights of the rates in the two groups were analyzed on days 0, 4, 7, 10 and 14 (Fig. 2B and C). The results showed no significant differences in body weights between the two groups at different time points, and no significant differences in eye weights were found between the two groups at different time points.

Xanthatin inhibits corneal neovascularization in the alkali burn rat model

In order to examine the effect of xanthatin in corneal neovascularization in an animal model of corneal neovascularization, 10 µM xanthatin eye drops were used to treat neovascular keratitis in the corneal alkali burn animal model. Following establishment of the corneal alkali burn model, the rats were administered with 10 µM xanthatin eye drops or PBS eye drops for 14 days, following which changes in corneal neovascularization and inflammatory reactions were examined. The corneal slit lamp and corneal tissue changes in the PBS group and the 10 µM xanthatin treatment group at 0 and 14 days post-corneal alkali burn are shown in Fig. 3A. In the PBS group, the extent of corneal neovascularization increased significantly with time. By contrast, the corneal neovascularization in the 10 µM xanthatin eye drop group regressed in the 14 days. The corneal tissue hematoxylin and eosin staining showed that the number of red blood cells in the corneal stroma layer was increased significantly at 14 days in the PBS group. However, the number of red blood cells in the corneal stromal layer of the 10 µM xanthatin treatment group was minimal. The results in the PBS group and the 10 µM xanthatin group at 0, 4, 7 and 10 days post-alkali burn, and changes in the inflammatory indices in the 14 days are shown in Fig. 3B. The results showed that, in the 10 µM xanthatin treatment group, the inflammatory index was significantly lower, compared with that in the PBS eye drop group at each time point. The inflammatory index showed a decreasing trend with time in the 10 µM xanthatin treatment group, indicating that 10 µM xanthatin effectively controlled the inflammatory response. The area of corneal neovascularization in the PBS group and the 10 µM xanthatin group was assessed at 0, 4, 7, 10 and 14 days, respectively (Fig. 3C). The results showed that the area of neovascularization in the 10 µM xanthatin treatment group was significantly lower, compared with that in the PBS treatment group, and there were significant differences at each time point. Therefore, 10 µM xanthatin inhibited alkali-induced neovascularization.

Xanthatin affects the expression of VEGF and PEDF in the cornea of the alkali burn model

In order to further examine the mechanism of inhibition of corneal neovascularization by xanthatin, western blot analysis and immunofluorescence were used to assess the expression of VEGF and PEDF in the corneal tissue of the PBS group and the 10 µM xanthatin treatment group at 14 days post-alkali burn (Fig. 4A–D). The results showed that the expression of VEGF in the cornea increased significantly in the 14 days in the PBS group. However, the expression of VEGF in the corneal tissue was significantly lower in the 10 µM xanthatin treatment group, compared with that in the PBS group. The expression level of PEDF in the PBS group decreased significantly in the 14 days, whereas the expression of PEDF in the 10 µM xanthatin group was significantly higher, compared with that in the PBS group. Therefore, the inhibition of corneal neovascularization by xanthatin in the alkali burn model was achieved by the downregulation of VEGF and upregulation of PEDF.

Xanthatin inhibits corneal neovascularization through VEGFR2/STAT3/PI3K

To elucidate the mechanisms underlying the inhibition of corneal neovascularization by xanthatin, the present study examined the expression of VEGFR2, an important receptor of VEGF, and its downstream associated molecules. The subcellular localization of STAT3 was detected in HUVECs using immunofluorescence under different conditions. A higher protein expression level of STAT3 was found in the VEGF-treated HUVECs, compared with that in the xanthatin+VEGF-treated HUVECs, in which the protein expression level of STAT3 decreased significantly (Fig. 5). It was concluded that xanthatin significantly downregulated the nuclear expression level of STAT3 in the VEGF-treated HUVECs. The expression levels of VEGFR2 were also compared between the two experimental groups using western blot analysis (Fig. 6A and B). The results showed that the expression level of t-VEGFR2 in the xanthatin-treated group was significantly lower, compared with that in the PBS group, whereas no significant difference was observed in the protein expression level of p-VEGFR2. The expression levels of signaling molecules downstream of VEGFR2, including STAT3, PI3K and Akt, were also examined (Fig. 6C and D). The results showed that the expression levels of p-STAT3, p-PI3K and p-Akt in the xanthatin group were significantly lower, compared with those in the PBS group. However, no significant differences were observed in the expression levels of t-STAT3, t-PI3K or t-Akt between the two experimental groups. It was concluded that xanthatin inhibited corneal neovascularization following alkali burn through the VEGFR2/STAT3/PI3K signaling pathway (Fig. 7).

Discussion

Normal corneal tissue is completely transparent and has no blood vessels. The surrounding vessels terminate at the limbus and form a vascular network, which provides nutrition to the cornea. The absence of vascularization is the main feature of the cornea. Infection, trauma, transplantation rejection and other factors can disrupt the balance of corneal avascularity and induce the process of angiogenesis (15). The changes of the cornea external environment can cause the formation of new blood vessels (16). Corneal neovascularization often leads to loss of corneal transparency (17). It is an important cause of blindness, and it is also a high-risk factor in rejection following corneal transplantation (18). The effective control of corneal inflammation and neovascularization in corneal diseases has become the main direction of treatment.

Xanthatin is the effective bioactive constituent of Xanthium sibiricum. In Chinese medicine, it is reported to be important in the treatment of colds, limb spasms and ulcer sores, and has antitumor, antiviral and antifungal effects (4). Compared with conventional anticancer therapy, the first generation of anti-angiogeneic drugs currently in clinical development have no clinical effects, therefore, the development of a more potent angiogenic inhibitor has become a promising focus of research. Studies have shown that xanthatin can inhibit tumor angiogenesis, limit its nutritional supply, inhibit tumor cell proliferation and induce the apoptosis of tumor cells, therefore, it can be used as a novel type of antitumor drug (19). Xanthatin (xanthium pavilion) has been shown to exhibit high in vitro cytotoxic activity in mouse lymphocytic leukemia cell lines (P-388 and L-1210) and human bronchial epidermoid tumor cells (NSCL-N6). However, in in vivo experiments, it exhibited weak cytotoxic activity (20). Previous experimental results show that xanthatin can inhibit the proliferation of HUVECs (12). The present study examined the effects of xanthatin on the migration properties and luminal formation of HUVECs. The results showed that xanthatin inhibited the migration of HUVECs, the number of luminal formations and the length of HUVECs. These results support the hypothesis that xanthatin can inhibit corneal neovascularization. In order to confirm these findings, based on the classic rat corneal alkali burn model, the present study used xanthatin eye drops as the route of treatment to confirm the effects and mechanism underlying the effects of xanthatin on the inhibition of inflammation and angiogenesis. The results showed that the extent of corneal neovascularization in the xanthatin treatment group was decreased significantly in the alkali-burned rats, compared with that in the control group. Using hematoxylin and eosin staining for the analysis of corneal tissues, the results showed that, in 14 days, the corneal stroma of the PBS eye drop-treated rats exhibited numerous red blood cells, whereas no obvious red blood cells were visible in the corneal stroma of the xanthatin treatment group. The inflammatory index was also used as a monitoring index. The results showed that the inflammatory index of the cornea decreased gradually with the prolongation of time, whereas no significant decrease was observed in the inflammatory index of the PBS eye group. The inflammatory index of the xanthatin eye drop group was significantly different from that of the PBS eye drop group at each time point. The area of corneal neovascularization area in the xanthatin-treated group was significantly smaller than that of the PBS group, and there were significant differences at each time point. Therefore, xanthatin inhibited neovascularization and inflammation in the corneas of the alkali-burned rats.

Previous studies have confirmed that VEGF and PEDF in the normal cornea are positively expressed, but that the normal cornea maintains transparency and avascularity, possibly due to the anti-angiogenic factor PEDF and the effect of angiogenesis induced by VEGF (15,21). Therefore, VEGF and PEDG are important in the formation of neovascularization following alkali burn. Under normal physiological conditions, there is a dynamic balance between VEGF and PEDF, which prevents neovascularization (22). In the present study, the expression levels of VEGF and PEDF in the corneal tissues of rats exposed to alkali burn were detected using immunofluorescence and western blot analysis. The results showed that, compared with the PBS eye drop group, the expression level of VEGF in the cornea of the xanthatin eye drop group was markedly decreased, whereas the expression level of PEDF was significantly increased. These results suggested that xanthatin inhibited neovascularization in the cornea of alkali-burned rats via the downregulation of VEGF and upregulation of PEDF.

Angiogenesis occurs through endothelial cell proliferation, differentiation, migration and lumen formation. This process is caused by a variety of factors, and VEGF is one of the most important regulatory factors. The VEGFR family is composed of three receptors, VEGFR1, VEGFR2 and VEGFR3, and VEGFR2 is involved in the main process of mediating VEGF-induced neovascularization (23). It has been shown that the VEGF/VEGFR2 signaling pathway is key in corneal neovascularization (24). In the present study, xanthatin significantly inhibited the expression of VEGF in the cornea of alkali-burn model rats. In the in vitro experiments, it was confirmed that xanthatin also downregulated the VEGF-induced expression level of VEGFR2 in HUVECs. From this, it was concluded that xanthatin regulated corneal neovascularization by regulating the VEGF/VEGFR2 signaling pathway.

STAT3 is a transcription factor, which can be activated by a variety of cytokines and growth factors. Following activation, STAT3 is translocated into the nucleus and regulates apoptosis, proliferation, migration and the expression of associated genes in the nucleus (25). Studies have shown that inhibition of the STAT3 signaling pathway mediated by VEGFR2 is an effective way of inhibiting neovascularization and tumor proliferation (26,27). In tumor vascular endothelial cells, the VEGFR2 pathway upregulates STAT3, causing the translocation of STAT3 into the nucleus, resulting in tumor angiogenesis (28). In retinal microvascular endothelial cells, small interfering RNA-mediated knockdown inhibits the activation of VEGFR2 and phosphorylation of STAT3 protein, which inhibits the proliferation of microvascular endothelial cells and the formation of pathological neovascularization (29). In a murine melanoma model, inhibition of the protein expression of VEGFR2 downregulated the phosphorylation of STAT3 protein and its nuclear localization, which inhibited tumor proliferation and neovascularization (30). Therefore, the VEGFR2/STAT3 signaling pathway is important in angiogenesis. In the present study, the effects of xanthatin on the subcellular localization and the phosphorylation level of STAT3 were determined in VEGF-induced HUVECs. The results showed that xanthatin significantly reduced the intracellular localization of STAT3 in the HUVECs and a significant decrease in the phosphorylation level of STAT3 was detected. It was concluded that xanthatin regulated the VEGFR2/STAT3 signaling pathway in the VEGF-induced HUVECs, and reduced the level and phosphorylation of nuclear STAT3, reducing the occurrence of neovascularization.

The PI3K/Akt signaling pathway is involved in a variety of important cellular processes, including cell growth, metabolism, survival and angiogenesis (31). This pathway is also key in the regulation of neovascularization by VEGF (32,33). The PI3K/Akt signaling pathway inhibits angiogenesis in malignant liver tumors, melanoma, hemangioma and renal cell carcinoma by regulating vascular endothelial remodeling (3437). The PI3K/Akt signaling pathway is an important downstream pathway of STAT3 protein, and the present study hypothesized that xanthatin inhibits corneal neovascularization by inhibiting the PI3K/Akt signaling pathway regulated by STAT3. Therefore, the present study investigated whether xanthatin affected the expression of the PI3K/Akt signaling pathway in VEGF-induced HUVECs. The results showed that xanthatin significantly inhibited the phosphorylation levels of PI3K and Akt. It was concluded that xanthatin inhibited the luminal formation and proliferation of HUVECs induced by VEGF through inhibition of the PI3K/Akt signaling pathway. Corneal neovascularization was inhibited by xanthatin in the alkali burn model, possibly through the same mechanism.

In conclusion, xanthatin effectively inhibited the formation of corneal neovascularization in alkali burn rats. The primary mechanism underlying the effect of xanthatin may be through downregulating the expression level of VEGFR2 to inhibit the nuclear localization and protein phosphorylation of STAT3, and inhibit the downstream activation of the PI3K/Akt signaling pathway. Xanthatin is expected to become a novel drug for inhibiting neovascularization in ophthalmology and other associated disciplines, providing novel direction in studies investigating the inhibition of neovascularization.

Funding

This study was supported by grants from the National Natural Science Foundation of China (grant nos. 81160118, 81460092, 81460372 and 81660152) to Professor Yi Shao.

Availability of data and material

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

MS and YS conceived the concept of the study. LY and XZZ designed the study. QY, XZZ, QCY and CS analyzed the experiment data. MS, PWZ, NJ and MYM performed the experiment. XZZ wrote the manuscript and was involved in revising it critically for important intellectual content. All authors discussed the results and implications and commented on the manuscript at all stages.

Ethics approval and consent to participate

The present study was approved by the Medical Ethics Committee of The First Affiliated Hospital of Nanchang University (Nanchang, China).

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Acknowledgments

Not applicable.

References

1 

Ellenberg D, Azar DT, Hallak JA, Tobaigy F, Han KY, Jain S, Zhou Z and Chang JH: Novel aspects of corneal angiogenic and lymphangiogenic privilege. Prog Retin Eye Res. 29:208–248. 2010. View Article : Google Scholar : PubMed/NCBI

2 

West SK: Blinding trachoma: Prevention with the safe strategy. Am J Trop Med Hyg. 69(5 Suppl): S18–S23. 2003. View Article : Google Scholar

3 

Tutin TG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM and Webb DA: Flora Europea. Volume 4. Plantaginaceae to Compositae (and Rubiaceae). Cambridge University Press; Cambridge: 1976

4 

Romero M, Zanuy M, Rosell E, Cascante M, Piulats J, Font-Bardia M, Balzarini J, De Clerq E and Pujol MD: Optimization of xanthatin extraction from Xanthium spinosum L. and its cytotoxic, anti-angiogenesis and antiviral properties. Eur J Med Chem. 90:491–496. 2015. View Article : Google Scholar

5 

Li WD, Wu Y, Zhang L, Yan LG, Yin FZ, Ruan JS, Chen ZP, Yang GM, Yan CP, Zhao D, et al: Characterization of xanthatin: Anticancer properties and mechanisms of inhibited murine melanoma in vitro and in vivo. Phytomedicine. 20:865–873. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Tao L, Sheng X, Zhang L, Li W, Wei Z, Zhu P, Zhang F, Wang A, Woodgett JR and Lu Y: Xanthatin anti-tumor cytotoxicity is mediated via glycogen synthase kinase-3β and β-catenin. Biochem Pharmacol. 115:18–27. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Pinel B, Landreau A, Seraphin D, Larcher G, Bouchara JP and Richomme P: Synthesis of reduced xanthatin derivatives and in vitro evaluation of their antifungal activity. J Enzyme Inhib Med Chem. 20:575–579. 2005. View Article : Google Scholar

8 

Chandel S, Bagai U and Vashishat N: Antiplasmodial activity of Xanthium strumarium against Plasmodium berghei-infected BALB/c mice. Parasitol Res. 110:1179–1183. 2012. View Article : Google Scholar

9 

Waltenberger J, Claesson-Welsh L, Siegbahn A, Shibuya M and Heldin CH: Different signal transduction properties of KDR and Flt1, two receptors for vascular endothelial growth factor. J Biol Chem. 269:26988–26995. 1994.PubMed/NCBI

10 

Quinn TP, Peters KG, De Vries C, Ferrara N and Williams LT: Fetal liver kinase 1 is a receptor for vascular endothelial growth factor and is selectively expressed in vascular endothelium. Proc Natl Acad Sci USA. 90:7533–7537. 1993. View Article : Google Scholar : PubMed/NCBI

11 

Gille H, Kowalski J, Li B, LeCouter J, Moffat B, Zioncheck TF, Pelletier N and Ferrara N: Analysis of biological effects and signaling properties of Flt-1 (VEGFR-1) and KDR (VEGFR-2). A reassessment using novel receptor-specific vascular endothelial growth factor mutants. J Biol Chem. 276:3222–3230. 2001. View Article : Google Scholar

12 

Bernatchez PN, Soker S and Sirois MG: Vascular endothelial growth factor effect on endothelial cell proliferation, migration, and platelet-activating factor synthesis is Flk-1-dependent. J Biol Chem. 274:31047–31054. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Yu Y, Yu J, Pei CG, Li YY, Tu P, Gao GP and Shao Y: Xanthatin, a novel potent inhibitor of VEGFR2 signaling, inhibits angiogenesis and tumor growth in breast cancer cells. Int J Clin Exp Pathol. 8:10355–10364. 2015.PubMed/NCBI

14 

D'Amato RJ, Loughnan M, Flynn E and Folkman J: Thalidomide is an inhibitor of angiogenesis. Proc Natl Acad Sci USA. 91:4082–4085. 1994. View Article : Google Scholar : PubMed/NCBI

15 

Chang JH, Garg NK, Lunde E, Han KY, Jain S and Azar DT: Corneal neovascularization: An anti-VEGF therapy review. Surv Ophthalmol. 57:415–429. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Eliason JA: Leukocytes and experimental corneal vascularization. Invest Ophthalmol Vis Sci. 17:1087–1095. 1978.PubMed/NCBI

17 

Qazi Y, Wong G, Monson B, Stringham J and Ambati BK: Corneal transparency: Genesis, maintenance and dysfunction. Brain Res Bull. 81:198–210. 2010. View Article : Google Scholar

18 

Coster DJ and Williams KA: The impact of corneal allograft rejection on the long-term outcome of corneal transplantation. Am J Ophthalmol. 140:1112–1122. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Nibret E, Youns M, Krauth-Siegel RL and Wink M: Biological activities of xanthatin from xanthium strumarium leaves. Phytother Res. 25:1883–1890. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Wang L, Wang J, Li F, Liu X, Chen B, Tang YX and Wang MK: Cytotoxic sesquiterpene lactones from aerial parts of xanthium sibiricum. Planta Med. 79:661–665. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Matsui T, Nishino Y, Maeda S and Yamagishi S: PEDF-derived peptide inhibits corneal angiogenesis by suppressing VEGF expression. Microvasc Rec. 84:105–108. 2012. View Article : Google Scholar

22 

Roussakis C, Chinou I, Vayas C, Harvala C and Verbist JF: Cytotoxic activity of xanthatin and the crude extracts of xanthium strumarium. Planta Med. 60:473–474. 1994. View Article : Google Scholar : PubMed/NCBI

23 

Ohno-Matsui K, Morita I, Tombran-Tink J, Mrazek D, Onodera M, Uetama T, Hayano M, Murota SI and Mochizuki M: Novel mechanism for age-related macular degeneration: An equilibrium shift between the angiogenesis factors VEGF and PEDF. J Cell Physiol. 189:323–333. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Ferrara N: Vascular endothelial growth factor: Basic science and clinical progress. Endocr Rev. 25:581–611. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Chen WL, Chen YM, Chu HS, Lin CT, Chow LP, Chen CT and Hu FR: Mechanisms controlling the effects of bevacizumab (avastin) on the inhibition of early but not late formed corneal neovascularization. PLoS One. 9:e942052014. View Article : Google Scholar : PubMed/NCBI

26 

Kortylewski M and Yu H: Role of Stat3 in suppressing anti-tumor immunity. Curr Opin Immunol. 20:228–233. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Vesely DL: Cardiac hormones for the treatment of cancer. Endocr Relat Cancer. 20:R113–R125. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Vesely DL: Family of peptides synthesized in the human body have anticancer effects. Anticancer Res. 34:1459–1466. 2014.PubMed/NCBI

29 

Arulanandam R, Batenchuk C, Angarita FA, Ottolino-Perry K, Cousineau S, Mottashed A, Burgess E, Falls TJ, De Silva N, Tsang J, et al: VEGF-mediated induction of RD1-BF1/Blimp1 expression sensitizes tumor vasculature to oncolytic virus infection. Cancer Cell. 28:210–224. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Yang Z, Wang H, Jiang Y and Hartnett ME: VEGFA activates erythropoietin receptor and enhances VEGFR2-mediated pathological angiogenesis. Am J Pathol. 184:1230–1239. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Kamran MZ and Gude RP: Pentoxifylline inhibits melanoma tumor growth and angiogenesis by targeting STAT3 signaling pathway. Biomed Pharmacother. 67:399–405. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Marone R, Cmiljanovic V, Giese B and Wymann MP: Targeting phosphoinositide 3-kinase: Moving towards therapy. Biochim Biophys Acta. 1784:159–185. 2008. View Article : Google Scholar

33 

Cai J, Ahmad S, Jiang WG, Huang J, Kontos CD, Boulton M and Ahmed A: Activation of vascular endothelial growth factor receptor-1 sustains angiogenesis and Bcl-2 expression via the phosphatidylinositol 3-kinase pathway in endothelial cells. Diabetes. 52:2959–2968. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Alvarez Y, Astudillo O, Jensen L, Reynolds AL, Waghorne N, Brazil DP, Cao Y, O'Connor JJ and Kennedy BN: Selective inhibition of retinal angiogenesis by targeting PI3 kinase. PLoS One. 4:e78672009. View Article : Google Scholar : PubMed/NCBI

35 

Ji Y, Chen S, Li K, Li L, Xu C and Xiang B: Signaling pathways in the development of infantile hemangioma. J Hematol Oncol. 7:132014. View Article : Google Scholar : PubMed/NCBI

36 

Posadas EM, Limvorasak S, Sharma S and Figlin RA: Targeting angiogenesis in renal cell carcinoma. Expert Opin Pharmacother. 14:2221–2236. 2013. View Article : Google Scholar : PubMed/NCBI

37 

Zhu YJ, Zheng B, Wang HY and Chen L: New knowledge of the mechanisms of sorafenib resistance in liver cancer. Acta Pharmacol Sin. 38:614–622. 2017. View Article : Google Scholar : PubMed/NCBI

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August-2018
Volume 42 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Shen M, Zhou XZ, Ye L, Yuan Q, Shi C, Zhu PW, Jiang N, Ma MY, Yang QC, Shao Y, Shao Y, et al: Xanthatin inhibits corneal neovascularization by inhibiting the VEGFR2‑mediated STAT3/PI3K/Akt signaling pathway. Int J Mol Med 42: 769-778, 2018
APA
Shen, M., Zhou, X., Ye, L., Yuan, Q., Shi, C., Zhu, P. ... Shao, Y. (2018). Xanthatin inhibits corneal neovascularization by inhibiting the VEGFR2‑mediated STAT3/PI3K/Akt signaling pathway. International Journal of Molecular Medicine, 42, 769-778. https://doi.org/10.3892/ijmm.2018.3646
MLA
Shen, M., Zhou, X., Ye, L., Yuan, Q., Shi, C., Zhu, P., Jiang, N., Ma, M., Yang, Q., Shao, Y."Xanthatin inhibits corneal neovascularization by inhibiting the VEGFR2‑mediated STAT3/PI3K/Akt signaling pathway". International Journal of Molecular Medicine 42.2 (2018): 769-778.
Chicago
Shen, M., Zhou, X., Ye, L., Yuan, Q., Shi, C., Zhu, P., Jiang, N., Ma, M., Yang, Q., Shao, Y."Xanthatin inhibits corneal neovascularization by inhibiting the VEGFR2‑mediated STAT3/PI3K/Akt signaling pathway". International Journal of Molecular Medicine 42, no. 2 (2018): 769-778. https://doi.org/10.3892/ijmm.2018.3646