Identification of ETS-1 target genes in human fibroblasts

  • Authors:
    • Jens Claus Hahne
    • Ali Fuat Okuducu
    • Tanja Fuchs
    • Alexandra Florin
    • Nicolas Wernert
  • View Affiliations

  • Published online on: March 21, 2011     https://doi.org/10.3892/ijo.2011.981
  • Pages: 1645-1652
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Abstract

The transcription factor Ets-1 plays several distinct critical roles in tumour development and progression by acting both in neoplastic cells and in the tumour stroma. Increased expression of Ets-1 in tumours is often associated with a worse prognosis. Stromal fibroblasts attribute an important part to the behaviour of malignant tumours. In this study we investigated the role of Ets-1 in the tumour stroma. It is well known that ets-1 expression in fibroblasts - one of the main components of the tumour stroma - can be induced by basic fibroblast growth factor (bFGF). We applied suppression subtractive hybridization (SSH) to identify genes that are differentially expressed between bFGF stimulated wild-type fibroblasts and fibroblasts with reduced Ets-1 expression. We selected clones up- or down-regulated in bFGF stimulated wild-type fibroblasts using SSH and functionally characterized them by reference to public databases using NCBI BLAST tools. Expression levels of genes corresponding to subtracted clones were analyzed using RT-PCR. Known genes were associated with diverse functions; novel Ets-1 regulated genes identified by SSH not only encoded components involved in matrix degradation (as cathepsin and PAI-2) but also constituents of the extracellular matrix (ECM) including α-2-Type I collagen, TGF-β induced protein, lumican and decorin. Our findings identify several potential novel target genes of Ets-1, and they provide potentially important insights into the role of Ets-1 in stromal fibroblasts for both remodelling and different functionalities of the ECM.

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June 2011
Volume 38 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Hahne, J.C., Okuducu, A.F., Fuchs, T., Florin, A., & Wernert, N. (2011). Identification of ETS-1 target genes in human fibroblasts . International Journal of Oncology, 38, 1645-1652. https://doi.org/10.3892/ijo.2011.981
MLA
Hahne, J. C., Okuducu, A. F., Fuchs, T., Florin, A., Wernert, N."Identification of ETS-1 target genes in human fibroblasts ". International Journal of Oncology 38.6 (2011): 1645-1652.
Chicago
Hahne, J. C., Okuducu, A. F., Fuchs, T., Florin, A., Wernert, N."Identification of ETS-1 target genes in human fibroblasts ". International Journal of Oncology 38, no. 6 (2011): 1645-1652. https://doi.org/10.3892/ijo.2011.981