MicroRNA-1271 functions as a metastasis and epithelial-mesenchymal transition inhibitor in human HCC by targeting the PTP 4 A 1 / cSrc axis

MicroRNAs (miRNAs or miRs) have been shown to regulate hepatocellular carcinoma (HCC) metastasis. In the present study, we focused on the functions of miR-1271 in HCC metastasis. The downregulation of miR-1271 was found to be associated with to venous infiltration, an advanced TNM stage (III+IV stage) and a shorter survival time. Our in vitro and in vivo data demonstrated that miR-1271 prevented HCC cell migration and invasion, as well as the formation of lung metastatic clusters. In addition, miR-1271 was demonstrated to markedly inhibit the epithelial-mesenchymal transition (EMT) of HCC cells. Importantly, protein tyrosine phosphatase type IVA member 1 (PTP4A1) was identified as a direct downstream target of miR-1271 in HCC. Furthermore, we confirmed that the phosphorylation of c-Src at Tyr416 mediated by PTP4A1 was a potential anti-HCC mechanism of action of miR-1271. On the whole, our data indicate that miR-1271 inhibits HCC metastasis by targeting the PTP4A1/c-Src signaling pathway and may serve as a prospective cancer therapeutic target for HCC.


Introduction
The incidence and mortality associated with hepatocellular carcinoma (HCC) has increased in China, with >400,000 patients succumbing to the disease (1).HCC is becoming a major public health concern in China.Despite the use of modern therapies to improve the outcomes of patients with HCC, distant metastasis and a high rate of recurrence restrict the 5-year survival rate (2).Therefore, the identification of novel anti-metastasis targets is urgently required to improve the prognosis of patients with HCC patients.
MicroRNAs (miRNAs or miRs) are a class of endogenous, highly conserved, short non-coding RNAs (3).They can bind to the 3'-untranslated region (3'-UTR) of mRNAs to decrease target gene expression (4).Various cellular processes have been reported to be regulated by different miRNAs in HCC.miR-218 has been shown to decrease tumor growth (5), miR-26a to inhibit metastasis (6) and miR-214 to prevent angiogenesis (7) in HCC.Moreover, certain miRNAs, including miR-155 (8), miR-137 (9) and miR-135a (10) have been shown to be associated with the recurrence of and poor survival in HCC.Hence, miRNAs have the potential to act as biomarkers of and therapeutic targets in HCC.
Mounting evidence indicates that miR-1271, a newly identified miRNA, is downregulated in endometrial cancer, prostate cancer and myeloma (11)(12)(13).The re-induction of miR-1271 has been shown to inhibit proliferation in ovarian cancer (14) and invasion in pancreatic cancer (15).However, Wang et al (16) reported a promoting effect of miR-1271 in the regulation of non-small cell lung cancer (NSCLC) proliferation and invasion by targeting HOXA5.Thus, these data seem to suggest that miR-1271 is a 'friend or foe' depending on the cancer type.
The pathophysiological program that transforms epithelial cell types into cells with a mesenchymal phenotype is identified as epithelial-mesenchymal transition (EMT) (17).EMT frequently occurs in cancer cells, particularly in metastatic cells.EMT contributes to the progression of cancers from the initial to the advanced grade, and is considered a critical mechanism for the migration, invasion and distinct metastasis of cancer cells (18).
In the present study, we examined the functions of miR-1271 in metastasis and EMT in HCC.We found that the downregulation of miR-1271 was a common occurrence in HCC tissues.The downregulation of miR-1271 was significantly associated with venous infiltration, an advanced TNM stage and a short survival time of patients with HCC.Furthermore, miR-1271 was demonstrated to significantly inhibit tumor migration, invasion and EMT, as well as the formation of lung metastatic clusters of HCC by targeting protein tyrosine phosphatase type IVA member 1 (PTP4A1).

Materials and methods
Patients and cell lines.HCC tissues and matched non-tumor tissues were collected from 65 patients who underwent radical resection for HCC at the Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi'an Jiaotong University (Xi'an, China) between January 2011 and January 2013.None of the patient received any anticancer therapy prior to surgery.All patients underwent a complete 3-year follow-up, unless they succumbed to the disease before the end of the 3 years.Written informed consents were obtained from all patients enrolled in this study.Lentiviruses infection.miR-1271 vectors (miR-1271), negative control vectors (miR-ctrl), miR-1271 inhibitory vectors (anti-miR-1271) and negative control inhibitory vectors (anti-miR-ctrl) were purchased from GeneCopoeia (Guangzhou, China) and cloned into the pEZX-MR03 and pEZX-AM03 lentiviral expression vectors, respectively.MHCC97-H and Hep3B cells were infected with recombinant lentiviruses at an MOI of 10.
Plasmids and siRNA transfection.PTP4A1 plasmids was obtained from GeneCopoeia and cloned into the pcDNA3.1 expression vectors (Invitrogen, Carlsbad, CA, USA).PTP4A1 specific siRNA and negative control siRNA were purchased from Guangzhou RiboBio Co., Ltd.(Guangzhou, China).The PTP4A1 expression vector or PTP4A1 siRNA were transfected into the HCC cells using Lipofectamine 3000 (Invitrogen) according to the manufacturer's instructions.Immunohistochemistry. Briefly, paraformaldehyde-fixed paraffin tumor tissue sections were performed for immunohistochemical staining.PTP4A1 primary antibodies were diluted at 1:100 with phosphate-buffered saline (PBS) to label the antigens at 4˚C overnight.Biotinylated goat anti-rabbit secondary antibodies (ZSGB-Bio, Beijing, China) were used to label the combined primary antibodies.Complexes were detected by HRP-streptavidin conjugates (ZSGB-Bio) and visualized by DAB (ZSGB-Bio).The final scores were calculated by the product of staining intensity and positive staining cell percentage as previously described (19).

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR
In vitro migration and invasion assays.For in vitro experiments, wound healing assay, Transwell migration assay and Transwell invasion assay were used to determine cell migration and invasion.For wound healing assay, a wound line across the middle of 6-well plates with confluent cells was created using a 200 µl sterile tip.The cells were cultured in reduced serum DMEM medium in a humidified 5% CO 2 incubator at 37˚C for 48 h, and then images were taken with a phase-contrast microscope. Transwell inserts (Nalge Nunc International, Penfield, New York, NY, USA) were coated with Matrigel (BD Biosciences, Franklin Lakes, NJ, USA) at 1 mg/ml on the upper layer for invasion assay.Uncoated inserts were used for migration assay.Briefly, 5x10 4 cells were seeded into the upper chamber with reduced serum DMEM medium.Subsequenlty, 750 µl DMEM medium containing 10% FBS was added to the lower chamber.The cells were incubated in a humidified 5% CO 2 incubator at 37˚C for 24 h.The chambers were fixed in 4% paraformaldehyde for 5 min and then stained with 0.3% crystal violet dye for 10 min.The cells on the upper layer were removed with a cotton swab.Migrating or invading cells were counted under a light microscope.
In vivo metastasis analysis.For the in vivo metastasis model, 16 BALB/cA, 4-6-week-old, 16-18 g, female nude mice were housed in sterilized cages with appropriate environment (25˚C, 45% humidity) and fed a regular chow diet with water ad libitum.All animal protocols were approved by the Biomedical Ethics Committee of Xi'an Jiaotong University Health Science Center.The mice were randomly divided into 4 groups as follows: i) those injected with MHCC97-H cells transfected with miR-1271 overexpression vector; ii) those injected with MHCC97H cells transfected with miRctrl vector; iii) those injected with Hep3B cells transfected with anti-miR-1271 vector; and iv) those injected with Hep3B cells transfected with anti-miR-ctrl vector.The cells were suspended in cold 1X PBS buffer at a concentration of 1x10 5 /ml.Each mouse was injected with a 100 µl cell suspension (~1x10 4 cells) into the tail vein very slowly to establish the lung metastasis model.After 28 days, the weight of all mice increased up to 20-23 g.The mice were sacrificed by CO 2 euthanasia (the flow rate of CO 2 was 20% displacement/min).The lungs were then removed and formalin-fixed, paraffin-embedded sections were created for H&E staining (Heart Biological Technology Co., Ltd.).
Dual-Luciferase reporter assay.Two online MicroRNA targets prediction tools, TargetScan (http://www.targetscan.org/) and MiRanda (http://www.microrna.org/),were used to located the binding sites between miR-1271 and PTP4A1 3'-UTR region.The 3'-UTR sequence of PTP4A1 predicted to interact with miR-1271 and the designed mutant 3'-UTR sequence were synthesized and inserted into the pEZX-MT06 vector (GeneCopoeia).These two recombinant constructs were identified as wild-type 3'-UTR vector and mutant type 3'-UTR vector, respectively and transfected into the MHCC-97H cells.miR-1271 mimics (Guangzhou Ribobio) were transfected into these cells at the same time.After 48 h, the cells were harvested and luciferase activity was measured using the Luc-Pair™Duo-Luciferase Assay kit 2.0 (GeneCopoeia).Firefly luciferase activity was normalized to Renilla luciferase activity.
Statistical analyses.Continuous variables are presented as the means ± standard deviation (SD).Statistical analysis was performed using the SPSS version 21.0 software (SPSS Inc., Chicago, IL, USA) or GraphPad PRISM 5 software (GraphPad Software, La Jolla, CA, USA).Correlations between miR-1271 and clinicopathological data were analyzed by the Pearson's Chi-square test.The differences between groups were analyzed using a Student's t-test.One-way ANOVA was used to analyze the data from more than two groups.Bonferroni method was used as a post hoc test.Survival analysis was performed using a Kaplan-Meier curve and log-rank test.A value of P<0.05 was considered to indicate a statistically significant difference.

Downregulation of miR-1271 is associated with metastasis in human HCC.
We analyzed miR-1271 expression by RT-qPCR and found that it was frequently downregulated in HCC tissues (P= 0.007; Fig. 1A).Consistent with our findings, miR-1271 was also discovered to be markedly downregulated in 271 HCC tissues obtained from the TCGA miRNA-Seq data set (P<0.001; Fig. 1B).These findings encouraged us to further demonstrate a lower expression of miR-1271 in metastatic HCC tissues compared with non-metastatic tissues (P=0.004;Fig. 1C).Moreover, we determined the expression levels of miR-1271 in 4 HCC cell lines and LO2 cells.As shown in Fig. 1D, a significant change was confirmed by one-way ANOVA analysis in these 5 cell lines (P<0.05).Furthermore, all 4 HCC cell lines expressed decreased levels of miR-1271 compared with the LO2 cells (P<0.05,respectively).

Inhibitory effects of miR-1271 on metastasis and EMT in HCC.
After examining the expression of miR-1271 in different HCC cell lines, we overexpressed miR-1271 in MHCC97-H cells by transfection with miR-1271 lentivirus, and knocked down its expression levels in Hep3B cells by transfection with anti-miR-1271 lentivirus (data not shown).Wound healing assays were used to demonstrate that miR-1271 overexpression inhibited the migration of the MHCC97-H cells (P= 0.021; Fig. 2A), whereas miR-1271 knockdown promoted the migration of the Hep3B cells (P= 0.031; Fig. 2B).The inhibitory effect of miR-1271 on HCC cell migration was also be confirmed by a Transwell chamber model (P= 0.030 and P=0.034; Fig. 2C and D).Similarly, in the Transwell chamber invasion assays, the upregulation of miR-1271 decreased the invasion of the MHCC97-H cells (P= 0.009; Fig. 2E) and the downregulation of miR-1271 increased the invasion of the Hep3B cells (P=0.031;Fig. 2F).
To further investigate the effects of miR-1271 on tumor metastasis in vivo, we established a lung metastasis model by tail vein injection of HCC cells, and the maximum number of lung metastatic clusters number of a single lung we found was measured and found to be 19.As shown in Fig. 3A, the total number of lung metastatic clusters in the miR-1271 group was much lower than that in the miR-ctrl group (P=0.007).By contrast, a promoting effect on in vivo tumor metastasis induced by miR-1271 knockdown was observed in nude mice injected with recombinant Hep3B cells (P= 0.029; Fig. 3B).Taken together, these results demonstrated that miR-1271 significantly inhibited the metastasis of HCC cells in vitro and in vivo.
As already discussed above, EMT serves as a microfoundation for cancer metastasis.Thus, we evaluated whether miR-1271 regulates the EMT phenotype of HCC cells.As shown in Fig. 3C, the results from western blot analysis revealed that the re-induction of miR-1271 in the MHCC97-H cells increased the expression of epithelial markers (E-cadherin, P<0.05) but decreased the expression levels of mesenchymal markers (N-cadherin and vimentin; P<0.05, respectively).On the contrary, miR-1271 knockdown induced EMT by decreasing E-cadherin expression and increasing the expression of two mesenchymal markers expression (Fig. 3D; P<0.05, respectively).
PTP4A1 is a direct downstream target of miR-1271.To investigate the targets through which miR-1271 suppresses HCC metastasis, we used two online databases (TargetScan, http:// www.targetscan.org/and MiRanda http://www.microrna.org/)to predict that PTP4A1 was a high potential target of miR-1271 in HCC (Context++ score percentile, 99%; miRSVR score: -0.7645; Fig. 4A).To confirm this prediction, we first used a Dual-Luciferase reporter system to demonstrate that miR-1271, as we had expected, suppressed the luciferase activity of PTP4A1 with a wild-type 3'-UTR, but did not suppress the activity of PTP4A1 containing a mutant type 3'-UTR (P= 0.035; Fig. 4B).In addition, the results from RT-qPCR and western blot analysis revealed that miR-1271 negatively modulated the mRNA and protein levels of PTP4A1 in the HCC cells (P<0.05,respectively; Fig. 4C and D).Importantly, our study revealed a negative correlation between miR-1271 levels and PTP4A1 protein expression in 65 HCC tissues (r=-0.552,P<0.001; Fig. 4E).On the whole, these results strongly indicate that PTP4A1 is a target of miR-1271 in HCC.

miR-1271 prevents HCC metastasis by inducing c-Src phosphorylation.
To explore the underlying mechanisms of PTP4A1 in the regulation of HCC metastasis, we used western blot analysis to detect the expression of total c-Src expression, the phosphorylation of c-Src and two metastasis-associated c-Src downstream targets (Snail and MMP-9).As we had expected, the expression levels of c-Src Y416 , Snail and MMP-9 were downregulated in the MHCC97-H cells that stably overexpressed miR-1271 (P<0.05 and P<0.01, respectively; Fig. 7A, left lanes), and were upregulated in the Hep3B cells in which miR-1271 was inhibited (P<0.05 and P<0.01, respectively;   Fig. 7A, right lanes).By contrast, the expression levels of these genes were increased following transfection with PTP4A1 expression vector (P<0.05 and P<0.01, respectively; Fig. 7B, left lanes).Moreover, transfection with si-PTP4A1 decreased the expression levels of these genes, which was similar to the effects induced by miR-1271 overexpression (P<0.05 and P<0.01, respectively; Fig. 7B, right lanes).These results thus indicate that the multiple anticancer functions of miR-1271 are attributed to the inhibition of the PTP4A1/c-Src signaling pathway (Fig. 7C).

Discussion
The dysregulation of miRNA expression is a common incident in the development and progression of human cancers, including HCC (20).In the present study, we investigated the role of miR-1271 in HCC and discovered the following: First, miR-1271 was identified as a downregulated miRNA in HCC samples and cell lines.This finding was still consistently observed in the 271 HCC tissues from the TCGA database.We then found that the 23 HCC tissues from patients with metastasis expressed lower miR-1271 levels than other 42 HCC tissues.The intrahepatic and hematogenous metastasis of HCC attributed to the poor prognosis of patients with HCC.miRNAs have been recognized as important regulators of human cancer metastasis (21).Hence, in the present study, we analyzed our clinical data to discover that a low expression of miR-1271 was associated with tumor metastasis and a poor prognosis of patients with HCC.This prognostic value of miR-1271 in HCC was consistent with a previous result in neuroglioma (22).These results strongly indicated that miR-1271 is associated with tumor metastasis in HCC.
The biological functions of miR-1271 have been partly uncovered in other types of cancer.miR-1271 has been demonstrated to inhibit cell proliferation in oral squamous cell carcinoma (23) and to sensitize human gastric cancer cells to cisplatin treatment (24).In the present study, and to the best of our knowledge, we report for the first time that miR-1271 is a critical inhibitor of cell migration and invasion in HCC through gain-and loss-of-function experiments in vitro.Moreover, the overexpression of miR-1271 also suppressed the formation of lung metastatic clusters in mice.EMT is identified as loss of epithelial marker (E-cadherin) expression and an increase in mesenchymal marker (vimentin and N-cadherin) expression (25).EMT is involved in numerous liver disease states, such as HBV or HCV-associated viral hepatitis, non-alcoholic fatty liver disease, liver fibrosis and HCC (26).These diseases present EMT in hepatocytes and cancer cells, leading to cell migration or invasion.Therefore, EMT has been recognized as a fundamental mechanism for HCC metastasis.In this study, we demonstrated that the overexpression of miR-1271 increased E-cadherin expression, and decreased N-cadherin and vimentin expression, whereas the inhibition of miR-1271 exerted the opposite effects.These findings suggest that miR-1271 has considerable potential for cancer therapy.
PTP4A1 (also known as phosphatase of regenerating liver 1) belongs to a subfamily of prenylated protein tyrosine phosphatases (PTPs) (27).Even the expression of PTP4A1 in human normal tissues has not been well characterized, Wang et al (28) found that the PTP4A1 mRNA levels were elevated in a number of tumor cell lines, including HeLa, SK-Lu-1 and several melanoma cell lines.Our results also showed that a high expression of PTP4A1 in HCC tissues was associated with lower overall survival and disease-free rates.Although the transcriptional regulation of PTP4A1 is not yet well understood, post-transcriptional regulation by miRNAs has been widely reported.PTP4A1 serves as a tumor suppressor target of miR-339-5p in colorectal (29), and miR-601 (30) and miR-944 (31) in breast cancer.In this study, PTP4A1 was demonstrated as an effective target of miR-1271 by the following evidence: First, miR-1271 only decreased luciferase activity in MHCC97-H cells carrying the wild-type 3'-UTR of PTP4A1.Second, miR-1271 overexpression decreased the mRNA and protein expression levels of PTP4A1 in MHCC97-H cells, whereas miR-1271 knockdown increased their expression levels in Hep3B cells.In addition, a negative correlation between miR-1271 and PTP4A1 protein expression was confirmed in 65 HCC tissue samples.Finally, altering PTP4A1 expression in HCC cells abrogated the effects induced by miR-1271.These results suggest that PTP4A1 is a downstream effector of miR-1271 functions in HCC.
The proto-oncogene, tyrosine-protein kinase Src (simply c-Src), has been linked to the PTP family.Liang et al (32,33) revealed that PTP4A3 promoted proliferation and invasion by the reduction of C-terminal Src kinase (Csk), leading to a decrease in Src-Tyr527.However, in multiple myeloma cells, PTP4A3 has been demonstrated to activate Src by increasing the phosphorylation at Tyr416, but not by decreasing the phosphorylation at Tyr527 (34).In A549 lung cancer cells, the inhibition of PTP4A1 directly decreased c-Src expression (35).Intriguingly, there were no significant changes in total c-Src expression levels or its phosphorylation status by PTP4A2 knockdown (36).Src activation induces Snail and MMP-9 expression (37,38).In this study, miR-1271 inhibited the phosphorylation of c-Src at Tyr416 (Y416), and Snail and MMP-9 expression.Moreover, PTP4A1 knockdown mimicked this function, whereas PTP4A1 overexpression abrogated it.Even though the detailed mechanisms underlying the activation of c-Src by PTP4A1 are not yet clear, it is unreasonable that PTP4A1 regulates Src activation directly.First, our result showed that PTP4A1, a phosphatase, increased c-Src phosphorylation at Y416.Second, Luo et al (39) were unable to obtain direct binding evidence by immunoprecipitation.These results indicate that miR-1271-mediated PTP4A1 inhibition deactivates c-Src through an indirect signaling pathway.
In conclusion, the present study demonstrates that the downregulation of miR-1271 in HCC tissues is associated with metastasis and a poor prognosis.miR-1271 is confirmed as a novel tumor metastasis and EMT inhibitor in HCC.The multiple anticancer functions of miR-1271 are attributed to the inhibition of the PTP4A1/c-Src signaling pathway.Taken together, our findings suggest that miR-1271 is a prospective therapeutic target for HCC.

Figure 1 .
Figure 1.The expression of miR-1271 is downregulated in HCC.(A) Differential expression of miR-1271 detected in 65 paired HCC tissues and matched nontumor tissues.(B) Downregulation of miR-1271 in 271 HCC tissues and 39 normal liver tissues from the TCGA data set.*** P<0.001, compared with non-tumor tissues.RPM, reads per million mapped reads.(C) The expression of miR-1271 was compared between non-metastatic and metastatic HCC tissues.** P<0.01, compared with non-metastatic HCC samples.(D) miR-1271 expression levels were detected in normal hepatic cells (LO2) and 4 HCC cell lines (MHCC97-H, Bel-7402, Huh-7 and Hep3B).* P<0.05, compared with LO2 cells.The values are presented as the means ± SD from triplicate experiments.

Figure 2 .
Figure 2. miR-1271 inhibits HCC cell migration and invasion in vitro.The upregulation of miR-1271 was demonstrated to inhibit MHCC97-H cell migration by (A) wound healing assay, (C) Transwell migration assay and invasion by (E) Transwell invasion assay.* P<0.05, compared with the miR-ctrl group.Downregulation of miR-1271 was demonstrated to promote Hep3B cell migration by (B) wound healing assay, (D) Transwell migration assay and invasion by (F) Transwell invasion assay.* P<0.05, ** P<0.01, compared with the anti-miR-ctrl group.All values are presented as the means ± SD from triplicate experiments.

Figure 3 .
Figure 3. miR-1271 suppresses HCC lung metastasis and EMT.(A) miR-1271 overexpression decreased the number of lung metastatic nodules; n=4; ** P<0.01, compared with the miR-ctrl group.(B) miR-1271 inhibition increased the number of lung metastatic nodules; n=4; * P<0.05, compared with the anti-miR-ctrl group.Metastatic nodule was labeled by black arrow.(C) Upregulation of miR-1271 induced the expression of E-cadherin, but inhibited the expression of N-cadherin and vimentin.* P<0.05, compared with the miR-ctrl group.(D) Downregulation of miR-1271 decreased the expression of E-cadherin, but increased the expression of N-cadherin and vimentin.* P<0.05, compared with the miR-ctrl group.The values in (C and D) are presented as the means ± SD from triplicate experiments.

Figure 4 .
Figure 4. PTP4A1 is a direct target of miR-1271 in HCC.(A) The putative binding set and corresponding mutant sequence in the 3'-UTR of PTP4A1 for miR-1271.(B) miR-1271 only decreased the luciferase activity that carried wild-type 3'-UTR of PTP4A1.* P<0.05, compared with the mutant-type group.(C) Upregulation of miR-1271 inhibited PTP4A1 mRNA and protein expression levels.* P<0.05, compared with the miR-ctrl group.(D) Downregulation of miR-1271 induced PTP4A1 mRNA transcription and protein expression.* P<0.05, compared with the anti-miR-ctrl group.The values in (B), (C) and (D) are presented as the means ± SD from triplicate experiments.(E) Negative correlations between miR-1271 and PTP4A1 expression in HCC tissues, r=-0.552,P<0.001.Positive staining cell is labeled by black arrows.

Figure 5 .
Figure 5. PTP4A1 expression levels are altered in HCC cells.(A) The mRNA and protein expression levels of PTP4A1 were increased by transfection with PTP4A1 expression vector.*** P<0.001, * P<0.05, compared with the negative control vector group.(B) siRNA transfection decreased PTP4A1 expression.*** P<0.001, * P<0.05, compared with the si-ctrl group.All values are presented as the means ± SD from triplicate experiments.

Figure 6 .
Figure 6.PTP4A1 alternations abrogate the effects of miR-1271 in HCC cells.Restoration of PTP4A1 increased (A) cell migration, (B) invasion and (C) EMT in miR-1271-overexpressing MHCC97-H cells.* P<0.05, ** P<0.01, compared with negative control vector group.PTP4A1 knockdown attenuated (D) migration, (E) invasion and (F) EMT in Hep3B cells in which miR-1271 was suppressed.* P<0.05, compared with the si-ctrl group.All values are presented as the means ± SD from triplicate experiments.

Figure 7 .
Figure 7. miR-1271 carries out its functions by inhibiting the PTP4A1/c-Src signaling pathway.(A) The expression levels of c-Src Y416 , Snail and MMP-9 were decreased by the upregulation of miR-1271 expression in MHCC97-H cells, but increased by the downregulation of miR-1271 expression in Hep3B cells.* P<0.05, ** P<0.01, compared with the miR-ctrl group and anti-miR-ctrl group.(B) Restoration of PTP4A1 increased the expression levels of c-Src Y416 , Snail and MMP-9 which were inhibited by miR-1271 overexpression; by contrast, PTP4A1 knockdown suppressed these expression levels.* P<0.05, ** P<0.01, compared with the negative control vector group and si-ctrl group.All values are presented as the means ± SD from triplicate experiments.(C) Diagrammatic sketch of the signaling pathway that miR-1271 arrests tumor metastasis and EMT on HCC.

Figure 8 .
Figure 8.The prognostic significance of miR-1271 and PTP4A1 for HCC patients was evaluated by Kaplan-Meier analysis.The patients in the miR-1271 low expression group had (A) a lower overall survival rate and (B) a shorter tumor-free survival time.Patients in the high PTP4A1 expression group had a worse (C) overall survival and (D) a tumor-free survival.

Table I .
Correlation between the clinicopathological characteristics and miR-1271 and PTP4A1 expression in patients with HCC