Involvement of P-glycoprotein in an in vitro blood-brain barrier model

  • Authors:
    • B Joly
    • V Lecureur
    • C Puozzo
    • A Guillouzo
    • O Fardel
  • View Affiliations

  • Published online on: November 1, 1996     https://doi.org/10.3892/ijo.9.5.1029
  • Pages: 1029-1033
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Abstract

The P-glycoprotein (P-gp) multidrug transporter is present at the luminal face of the brain capillary endothelial cells that contribute to the blood-brain barrier. To study its role in transendothelial anticancer drug transport, we made use of a co-culture system formed of bovine brain capillary endothelial cells and astrocytes which allows the in vitro maintenance of specialized properties of the brain endothelial cells, including expression of P-gp as assessed by Northern and Western blot analyses. Vinblastine, an anticancer drug substrate for P-gp and known not to enter the brain, was found to be poorly transferred across the endothelial cell monolayer. This low vinblastine transport was however strongly increased in the presence of verapamil, a well known P-gp blocker. Moreover, verapamil was shown to increase the accumulation of the anticancer drug in the brain endothelial cells through inhibition of drug efflux. These results suggest that P-gp activity evidenced in the co-culture model is involved in the low transendothelial transport of vinblastine, thus supporting the conclusion that P-gp expressed at the blood-brain barrier level may prevent xenobiotics, including anticancer drugs, from entering the central nervous system.

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November 1996
Volume 9 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Joly B, Lecureur V, Puozzo C, Guillouzo A and Fardel O: Involvement of P-glycoprotein in an in vitro blood-brain barrier model. Int J Oncol 9: 1029-1033, 1996
APA
Joly, B., Lecureur, V., Puozzo, C., Guillouzo, A., & Fardel, O. (1996). Involvement of P-glycoprotein in an in vitro blood-brain barrier model. International Journal of Oncology, 9, 1029-1033. https://doi.org/10.3892/ijo.9.5.1029
MLA
Joly, B., Lecureur, V., Puozzo, C., Guillouzo, A., Fardel, O."Involvement of P-glycoprotein in an in vitro blood-brain barrier model". International Journal of Oncology 9.5 (1996): 1029-1033.
Chicago
Joly, B., Lecureur, V., Puozzo, C., Guillouzo, A., Fardel, O."Involvement of P-glycoprotein in an in vitro blood-brain barrier model". International Journal of Oncology 9, no. 5 (1996): 1029-1033. https://doi.org/10.3892/ijo.9.5.1029