Open Access

TCRP1 induces tamoxifen resistance by promoting the activation of SGK1 in MCF‑7 cells

  • Authors:
    • Shuai Zhao
    • Xiaohua Li
    • Lei Yin
    • Lili Hou
    • Jing Lan
    • Xun Zhu
  • View Affiliations

  • Published online on: April 3, 2020     https://doi.org/10.3892/or.2020.7577
  • Pages: 2017-2027
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Tamoxifen is widely used as a highly effective drug for treating estrogen‑receptor (ER) alpha‑positive breast cancer. However, tamoxifen resistance developed during cancer treatment remains a significant challenge. Tongue cancer resistance‑related protein1 (TCRP1), which is recognized as a novel drug target, is related to chemo‑resistance in human cancers, moreover, it is often overexpressed in various cancer cells, such as in lung cancer, breast cancer, and tongue cancer. However, the effects of TCRP1 on tamoxifen‑resistant breast cancer cells and tissues are far from clear. The present study revealed that TCRP1 induced tamoxifen resistance in breast cancer cells. Western blotting, quantitative real‑time polymerase chain reaction (RT‑PCR) and immunohistochemical staining were performed to detect the expression level of TCRP1 in vivo and in vitro between primary breast cancer tissues and tamoxifen‑resistant breast cancer tissues. The data revealed that the expression of TCRP1 was upregulated in the tamoxifen‑resistant breast cancer tissues and human breast cancer cell line, MCF‑7. Further study revealed that knocking down TCRP1 inhibited the growth of MCF‑7 cells with tamoxifen‑resistance (MCF7‑R cells) and induced cell apoptosis. Moreover, TCRP1 promoted serum‑ and glucocorticoid‑inducible kinase 1 (SGK1) activation via phosphorylation of phosphoinositide‑dependent kinase 1 (PDK1) in MCF7‑R cells. In addition, it was also observed that knocking down TCRP1 inhibited tumorigenesis of MCF‑7 cells in nude mice. In conclusion, these data indicated that TCRP1 could induce tamoxifen resistance by regulating the PDK1/SGK1 signaling pathway. Thus, TCRP1 could be explored as a promising candidate for treating tamoxifen‑resistant breast cancer in the future.

Introduction

Breast cancer is a malignant tumor with the highest incidence and mortality for women in the world. Annually, approximately 1 million new cases of breast cancer are diagnosed worldwide (1). According to the global cancer statistics of 2018, the mortality of breast cancer accounted for 11.6% of all cancer-related mortalities (2).

Estrogen signaling pathway functions critically in the pathogenesis of breast cancer, and 75% of breast cancer patients are estrogen receptors (ER) alpha-positive and are generally treated by endocrine therapy (3,4). Tamoxifen, which is used as a first-line drug for endocrine therapy, has been extensively used to treat early and late ER+ breast cancer patients for the past three decades (5). The structure of tamoxifen is similar to that of estrogen, a selective estrogen receptor regulator (610). Although numerous breast cancer patients benefit from the drug, tamoxifen resistance developed during the therapy still remains a clinical challenge (11). Approximately 1/3 of patients develop resistance to tamoxifen at the beginning of treatment, and those who initially are sensitive to the drug will later develop resistance (12). Thus, investigating the mechanisms underlying the resistance and detecting the molecular mediators of tamoxifen are necessary, since it may help identify new strategies against tamoxifen-resistant cancer cells.

TCRP1, alternatively known as FAM168A, is located on human chromosome 11q13.4. It encodes 235 amino acids of putative protein and its molecular weight is 25 kDa (13). TCRP1 is used as a novel candidate inducing drug resistance, and it is widely expressed in numerous types of cancer cells (1416). In the past few years, certain studies have reported that TCRP1 promotes tumorigenesis in oral squamous cell carcinoma through the Akt signaling pathway (14,15). Several studies have reported that TCRP1 mediates DDP-resistant lung cancer cells by inhibiting the degradation of Pol b (16). Moreover, TCRP1 plays an indispensable role in promoting the transformation of NIH/3T3 cells by overexpressing PDK1 and AKT1 (17). However, the effects of TCRP1 on MCF7-R cells and the underlying mechanism are still unknown. The present research, aimed to explore whether TCRP1 mediated tamoxifen resistance in breast cancer and investigated the mechanism involved in the TCRP1-mediated tamoxifen resistance in breast cancer.

Materials and methods

Cell culture

The human breast adenocarcinoma cell line MCF-7 was purchased from the American Type Culture Collection. The MCF-7 cells were cultured in Dulbecco's modified Eagle medium (DMEM) (Invitrogen; Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (FBS) (Sigma-Aldrich; Merck KGaA) and 1% penicillin/strptomycin with 5% CO2 at 37°C. For the acquired tamoxifen-resistant breast cancer cells, MCF-7 cells were cultured in DMEM containing 10% FBS with 1 µM tamoxifen (cat. no. T5648; Sigma-Aldrich; Merck KGaA) for 3 months, and then an extra 3 µM tamoxifen was added into the medium, and incubated for more than 9 months (18). MCF-7 cells and those with tamoxifen resistance (MCF7-R cells) served as tumor cell models.

Quantitative real-time polymerase chain reaction (RT-PCR)

Total RNAs were extracted from MCF-7 cells and MCF7-R cells respectively using RNAiso plus reagent (code.no. 9109; Takara), and complementary DNAs (cDNAs) were reverse-transcribed using the PrimeScript™ RT Master Mix (Perfect Real Time) (code no. RR036) in a 10-µl reaction system, which consisted of 500 ng total RNAs, 2 µl 5X PrimeScript RT Master Mix (Perfect Real-Time), and 10 µl RNase-free ddH2O. The reaction was performed at 50°C for 15 min and at 85°C for 2 min. The cDNAs were diluted to a ratio of 1:4 by ddH2O before using. According to a q-PCR assay, 10 µl reaction systems (cat. no. 04913914001; Roche Applied Science), which were composed of 5 µl FastStart Universal SYBR Green Master, 2 µl cDNA, 2 µl ddH2O, and 1 µl primer, were performed. The results were analyzed by Rotor-Gene 5 software (Rotor-Gene; Corbett Research). The reaction was carried out at 50°C for 2 min and at 95°C for 10 min, then at 95°C for 20 sec, 65°C for 20 sec and 72°C for 30 sec for amplification, for a total of 40 cycles. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) RNA served as an internal reference. Primers used for quantitative real-time PCR were as follows: TCRP1 forward, 5′-CTCAGCCTCTTGCTGTGATG-3′ and reverse, 5′-AACTGTGCCCATCCTACCAG-3′; GAPDH forward, 5′-TGCACCACCAACTGCTTAGC-3′ and reverse, 5′-GGCATGGACTGTGGTCATGA-3′; SGK1 forward, 5′-GCAGAAGAAGTGTTCTATGCAGT-3′ and reverse, 5′-CCGCTCCGACATAATATGCTT-3′.

Western blotting

Proteins were lysed from a buffer containing Pierce Protease and Phosphatase Inhibitor Mini Tablets. The whole cell lysates were gently transferred from the plate into a 1.5-ml EP tube, lysed at 4°C for 30 min, and then centrifuged at 12,000 × g for 15 min at 4°C. The supernatant was collected from the newly labeled EP tube, and a small amount of the supernatant was used to assess the protein concentration using a BCA Protein Assay Kit (cat. no. 23235; Thermo Fisher Scientific, Inc.). The remaining supernatant was added to the 5X protein loading buffer at 1:4, and the proteins were boiled at 100°C for 5 min. Next, the proteins (30 µg) were electrophoretically separated on 10% SDS-PAGE, and transferred to polyvinylidene difluoride (PVDF) (cat. no. OPVH00010; EMD Millipore) membranes, which were then blocked by 5% non-fat milk for 1 h and incubated with a primary antibody at a dilution of 1:1,000 in 1X TBS buffer at 4°C overnight. After washing the membranes with 1X TBST three times, a secondary antibody conjugated with goat anti-rabbit (cat. no. A0208; Beyotime Institute of Biotechnology) or goat anti-mouse HRP at a dilution of 1:1,000 (cat. no. A0216; Beyotime Institute of Biotechnology) was incubated with the membranes at room temperature for 2 h. The membranes were washed by 1X TBST again, and protein signals were detected by ECL kit (cat. no. 34577; Thermo Fisher Scientific, Inc.) with a BioRad GS-800™ densitometer scanner (Bio-Rad Laboratories), and the data was analyzed by using PDQuest 7.2.0 software (Bio-Rad Laboratories). The antibodies used were as follows: TCRP1 (cat. no. HPA037580; rabbit; 1:200; Sigma-Aldrich; Merck KGaA), PCNA (cat. no. 13110; rabbit; 1:1,000), cleaved caspase-3 (cat. no. 9664; rabbit; 1:1,000), cleaved PARP (cat. no. 5625; rabbit; 1:1,000), p-PDK1 (cat. no. 3438; rabbit, 1:1,000), PDK1 (cat. no. 3062; rabbit, 1:1,000; all from Cell Signaling Technology, Inc.), p-SGK1 (cat. no. SAB4503834; rabbit; 1:1,000; Sigma-Aldrich; Merck KGaA), SGK1 (cat. no. S5188; rabbit; 1:2,000; Sigma-Aldrich; Merck KGaA), and β-actin (cat. no. 3700, mouse; 1:1,000; Cell Signaling Technology, Inc.).

Cytotoxicity assay

A single cell suspension was prepared from culture medium containing 10% FBS, and seeded into a 96-well plate at a volume of 5,000 cells/well. After culturing for 3 to 5 days under the same general culture conditions, 20 µl MTT solution (cat. no. ST316; Beyotime Institute of Biotechnology) (5 mg/ml with PBS) was added into each well. After incubating the plate at 37°C for 2 h, the culture was terminated, and the culture supernatant in the well was carefully discarded. DMSO (150 µl) was added into each well and shaken gently for 10 min to fully melt the crystals. The light absorption value of each well was measured at an absorbance of 490 nm using a microplate reader on an enzyme-linked immunosorbent monitor. Then the result was recorded, and a cell growth curve was plotted, with time as the abscissa and absorbance as the vertical coordinate.

Short hairpin RNA (shRNA) and plasmid and cell transfection

TCRP1 shRNA (forward primer, GGAAAUACAUAGACCUACA and reverse primer, UGUAGGUCUAUGUAUUUCC) oligonucleo-tides with 3′dTdT overhangs were synthesized by QIAGEN. Control shRNA in the experiments refers to a non-short hairpin (NSh) shRNA (NSF, UUCUCCGAACGUGUCACGU; NSR, ACGUGACACGUUCGGAGAA), which was designed and synthesized by QIAGEN. The TCRP1 overexpression vector pcDNA3.1-TCRP1 was constructed as previously described (13). The vector for overexpressing PDK1 (pcDNA3.1-PDK1) and SGK1 (pcDNA3.1-SGK1) were purchased from Shanghai GenePharma Co., Ltd. MCF7-R cells were transfected with shRNA or plasmids by using Lipofectamine 2000 reagent (cat. no. 11668019; Invitrogen; Thermo Fisher Scientific, Inc.). The expression levels of TCRP1, PDK1 and SGK1 were determined by western blotting and q-PCR.

Immunohistochemical staining

Fresh specimens of the primary breast cancer tissues and tamoxifen-resistant breast cancer tissues were collected from Wuzhong People's Hospital. All patients signed a written informed consent, and the present study was approved by the Ethics Committee of Wuzhong People's Hospital, Suzhou, China. The clinical data of patients are presented in Table I. A total of 40 samples were collected and divided into two groups, with 20 samples in each group. The tumor specimens were fixed by 4% neutral formalin, embedded in paraffin and sectioned into 4-µm thick sections by standard SP method. Immunostaining was performed with the avidin-biotin-peroxidase complex method (Ultrasensitive™; MaiXin). Then tissue sections were incubated with TCRP1 antibody (cat. no. HPA037580; rabbit; 1:200 Sigma-Aldrich; Merck KGaA), SGK1 antibody (cat. no. S5188; rabbit, 1:2,000; Sigma-Aldrich; Merck KGaA), Ki67 antibody (cat. no. 9129; rabbit; 1:400; Cell Signaling Technology, Inc.) overnight at 4°C, followed by incubation with a goat anti-rabbit biotinylated secondary antibody (cat. no. SA1020; dilution 1:1,000; Boster Bio) at 4°C overnight. The images were analyzed with IPLab 4.0 imaging software (Scanalytics, Inc.).

Table I.

Clinical features of primary breast cancer group and tamoxifen-resistance breast cancer group samples.

Table I.

Clinical features of primary breast cancer group and tamoxifen-resistance breast cancer group samples.

CharacteristicsAll patientsPrimary breast cancerTamoxifen-resistant breast cancer
Depth of invasion
  T1+T21596
  T3+T4251312
Lymph node metastasis
  N013310
  N1+N2+N3271413
Distant metastasis
  M0291316
  M11147
TNM stage
  I+II221111
  III+IV18126

[i] Results were obtained using χ2 test.

Clonogenic survival assay

MCF7-R cells were seeded at 1×103 cells/well into 6-well plates and cultured overnight. MCF7-R cells were treated by TCRP1 shRNA or shRNA control for 48 h. After growing for 10 days at 37°C, the colonies were formed, fixed with 4% paraformaldehyde, stained with 0.1% crystal violet (Sigma-Aldrich; Merck KGaA) for 15 min. Colonies containing 50 or more cells were counted under a fluorescence microscope (Leica Microsystems).

Flow cytometry

MCF7-R cells were seeded in a 100-mm plate at 1×106 cells and cultured overnight. Then, the cells were treated by TCRP1 shRNA, SGK1 overexpression and shRNA control for 48 h. An Annexin V-FITC Apoptosis Detection Kit (cat. no. AVK250; Strong Biotech Corporation) was used to detect the results. After incubation, the medium was removed, and the cells were washed with cold phosphate-buffered saline and then collected. The supernatant was removed by centrifugation at 300 × g for 5 min and then the cells were resuspended in 1X Annexin-binding buffer. Then 5 µl of Annexin V and 1 µl 100 µg/ml propidium iodide working solution were added to 100 µl of cell suspension and incubated with the cells at room temperature for 15 min. Next, 400 µl 1X Annexin-binding buffer was added, mixed gently and kept on ice. A flow cytometer (BD FACSCanto; BD Biosciences) was used to analyze the results. Apoptosis at different stages was explored by gating the respective population on the dot plots. There are four stages of cells, to be more specific, Q1 are necrosis cells Annexin V/PI+; Q2 are late apoptotic cells Annexin V+/PI+; Q3 are normal cells Annexin V/PI; and Q4 are early apoptosis cells Annexin V+/PI.

Animal studies

All animal studies were approved by the Animal Experimentation Ethics Committee of Wuzhong People's Hospital, Suzhou, China. The animal studies were performed according to corresponding protocols. BALB/c nude mice (n=30; weight, 18–20 g) (Taconic) aged between 4 to 6 weeks old, were used to construct a subcutaneous xenograft model. In brief, 1×105 MCF-7-vector cells and 1×105 MCF-7-shTCRP1 cells were subcutaneously inoculated on both sides of the rear back in mice (N=15 per group) (17). The tumor volume was measured and was indicative of tumor growth.

Statistical analysis

The data are presented as the mean ± SEM of at least three independent experiments. The statistical differences in xenograft tumor growth in response to MCF-7-shTCRP1 treatment were analyzed by one- or two-way ANOVA, followed by Bonferroni's test. Other P-values were subjected to two-tailed unpaired Student's t-test. P-values <0.05 were considered to indicate a statistically significant difference. The data were analyzed by Graphpad Prism5 (GraphPad Software, Inc.). Correlations among the expression levels of TCRP1 and PDK1, SGK1 were determined by Pearson's correlation. χ2 test and Student's t-test were used to compare the distribution of categorical and continuous variables, respectively.

Results

TCRP1 expression is increased in tamoxifen-resistant breast cancer tissues

To explore the potential function of TCRP1 in tamoxifen resistance, the expression of TCRP1 was detected in breast cancer tissues. RT-PCR results revealed that TCRP1 mRNA levels were particularly increased in the tamoxifen-resistant breast cancer samples compared with the primary breast cancer samples (Fig. 1A). Consistently, immunohistochemical staining data revealed that TCRP1 was significantly overexpressed in tamoxifen-resistant tissue samples compared with primary tissue samples (Fig. 1B and C).

TCRP1 induces tamoxifen resistance in MCF7-R cells

Next, it was investigated whether TCRP1 was also increased in MCF-7 cells and MCF7-R cells. Cell viabilities of the two cells exposed to tamoxifen (0–2 µM) for 48 h were detected by MTT assay. The dose-response curves were plotted, and the IC50 values of tamoxifen were determined. The present results revealed that the viability of MCF7-R cells was significantly less affected than that of MCF-7 cells under tamoxifen stimulation (IC50, 17.44 µM) (Fig. 2A). Next, the mRNA and protein levels of TCRP1 in MCF-7 cells and MCF7-R cells were detected by RT-PCR and western blotting, respectively. The present data revealed that the expression levels of TCRP1 at the mRNA and protein levels were increased in MCF7-R cells compared with MCF-7 cells (Fig. 2B-D). To further investigate whether TCRP1 was closely related to tamoxifen resistance, the effects of knockdown of TCRP1 and overexpression of TCRP1 were detected on MCF7-R cells. The expression of TCRP1 was determined by RT-PCR and western blotting. The results revealed the short hairpin TCRP1 (shTCRP1) and overexpression of TCRP1 were successfully realized at mRNA and protein levels (Fig. 2E-F), with shTCRP1#1 exhibiting the strongest knockdown effect, therefore shTCRP1#1 was used in the following experiments. The transfected MCF7-R cells were treated with 0 to 2 µM tamoxifen, and the result revealed that the IC50 value of TCRP1-overexpressed MCF7-R cells to tamoxifen was higher than the control cells (Fig. 2G). However, the opposite results were revealed in TCRP1-knockdown MCF7-R cells. The IC50 value of TCRP1-knockdown cells to tamoxifen was lower than that of shRNA control cells (Fig. 2H). These data indicated that overexpression of TCRP1 increased resistance to tamoxifen in MCF7-R cells, while knocking down TCRP1 significantly attenuated the tamoxifen resistance in MCF7-R cells.

Knockdown of TCRP1 decreases cell proliferation and promotes apoptosis in MCF7-R cells

It was revealed that TCRP1 induced the tamoxifen resistance in MCF7-R cells, however, the underlying mechanisms were still unclear. To explore the functions of TCRP1 in MCF7-R cells, MCF7-R cells were treated with shRNA for TCRP1 or shRNA control. An MTT assay and clonogenic survival assay were performed to evaluate the effects of TCRP1 on the proliferation of MCF7-R cells. The results revealed that knockdown of TCRP1 significantly decreased cell proliferation (Fig. 3A). Clonogenic survival assay analysis also revealed that clonogenic ability of TCRP1-knockdown cells was significantly attenuated compared with shRNA control cells (Fig. 3B and C). Flow cytometry was performed to examine whether TCRP1 was involved in cell apoptosis, and the results revealed that the proportion of apoptotic cells in the TCRP1-knockdown cells were significantly increased compared with the control cells (Fig. 3D and E). The expression levels of several key apoptosis-associated proteins were further detected, and the data revealed that the pro-apoptotic proteins, cleaved caspase-3 and cleaved PARP were significantly upregulated, and the level of the anti-apoptotic protein PCNA was downregulated in the TCRP1-knockdown cells (Fig. 3F and G). These findings indicated that knockdown of TCRP1 inhibited the growth of MCF7-R cells, and promoted cell apoptosis.

TCRP1 phosphorylates PDK1 and further activates PDK1/SGK1 signaling

It has been reported that TCRP1 can interact with PDK1, which promotes tumor growth and metastasis in a spontaneous breast cancer model (19). PDK1 activity was suppressed to inhibit downstream SGK1 phosphorylation and activity in BYL719-resistant cells (20). Therefore, it was explored whether TCRP1 induced tamoxifen resistance in the MCF7-R cells via PDK1/SGK1 signaling. MCF7-R cells were treated with shRNA for TCRP1 or shRNA control, respectively, while MCF7-R cells were transfected with pcDNA3.1-TCRP1 or control plasmid, respectively. The results revealed that phosphorylation levels of PDK1 and SGK1 were significantly increased in TCRP1-overexpressed cells, however, the expression levels of phosphorylated (p)-PDK1 and p-SGK1 were decreased in TCRP1-knockdown cells, compared with shRNA control cells (Fig. 4A and D). In order to further confirm the relationship between PDK1 and SGK1, MCF7-R cells were transfected with PDK1 expression vector or control vector. The results revealed that the p-PDK1 and p-SGK1 were significantly upregulated in MCF7-R cells (Fig. 4B and E). To elucidate the signaling pathway, a co-transfection system was constructed with PDK1 expression vector in combination with the TCRP1 shRNA into MCF7-R cells, and as revealed in Fig. 4C and F, phosphorylation of SGK1 was decreased in TCRP1-knockdown cells compared with shRNA control cells. However, after transfection of PDK1 expression vector into TCRP1-knockdown cells, the protein level of p-SGK1 was restored to the level similar to that of endogenous expression. These data indicated that TCRP1 could activate PDK1/SGK1 signaling by phosphorylating PDK1 and then further phosphorylating SGK1.

TCRP1 induces tamoxifen resistance by activating SGK1

It was further investigated whether SGK1 played a critical role in TCRP1-mediated tamoxifen resistance. MCF7-R cells were treated with SGK1 expression vector, or TCRP1 shRNA, or co-transfection, or control, respectively. The transfection efficiency was detected by q-PCR, and the efficiency of SGK1 was revealed to be significantly increased in the SGK1-overexpressed cells compared to the control cells (Fig. 5A). In TCRP1-knockdown cells, the phosphorylation of SGK1 was significantly decreased compared with the control cells. In addition, in TCRP1-knockdown cells treated by SGK1 expression vector, the expression of p-SGK1 was increased to the level similar to that of the endogenous expression (Fig. 5B and C). Moreover, cell viability was detected by MTT assays in the three groups under stimulation of 0 to 2 µM tamoxifen. The IC50 value of the TCRP1-knockdown cells was decreased compared to the control cells, however, the IC50 value of the co-transfected cells was increased compared with TCRP1-knockdown cells (Fig. 5D). Furthermore, flow cytometric results revealed that treatment with TCRP1 shRNA promoted cell apoptosis, however, when it was used in combination with overexpressed SGK1, apoptosis was decreased to the control level (Fig. 5E and F). These experiments demonstrated that TCRP1 induced tamoxifen resistance possibly by activating SGK1.

Knockdown of TCRP1 inhibits the growth of transplanted tumor in mice

TCRP1-knockdown MCF-7 cells and shRNA control MCF-7 cells were subcutaneously inoculated on both sides of the rear back in nude mice. After feeding the mice for 3 weeks, the treated mice were able to form xenografts. As revealed in Fig. 6A, tumor growth was significantly decreased in the mice treated by the TCRP1 shRNA group compared with that of the shRNA control group. Consistently, the size of control tumors was significantly increased compared to that of TCRP1-knockdown tumors (Fig. 6B). The results of the tumor weight revealed that the xenografts in the TCRP1-knockdown group had a significantly decreased growth rate compared with that of the control group (Fig. 6C). To further analyze related proliferation indexes of tumor growth, immunohistochemistry with Ki67 staining was performed. The results revealed that the amount of Ki67-positive staining in the TCRP1-knockdown group was significantly decreased compared to that of the control group (Fig. 6D). Moreover, the expression of TCRP1 and SGK1 was increased (Fig. 6D). Thus, these results indicated that TCRP1 inhibited the proliferation of MCF7-R cells in vitro and in vivo.

Discussion

The present study aimed to explore the function and mechanism involved in TCRP1-mediated tamoxifen resistance in MCF7-R cells. The present results revealed that TCRP1 plays a vital role in tamoxifen resistance in MCF7-R cells, and that knockdown of TCRP1 decreased tamoxifen resistance, inhibited the growth of MCF7-R cells, and promoted cell apoptosis. Moreover, it was observed that TCRP1 induced tamoxifen resistance by phosphorylating PDK1 and further activating PDK1/SGK1 signaling. Therefore, the present study provides strong evidence demonstrating that TCRP1 induces tamoxifen resistance possibly by promoting the activation of SGK1 in MCF7-R cells.

Clinical studies have revealed that high expression of TCRP1 was related to a poor prognosis for patients who experienced OSCC radioresistance (15). In addition, TCRP1 expression was observed to be significantly increased in lung cancer tissues and ovarian cancer tissues, and it could mediate DDP and L-OHP resistance in the cell lines of the two types of cancer (21). Moreover, consistent with upregulated TCRP1, c-Myc was also upregulated in both multidrug-resistant tongue cancer cell line Tca8113/PYM and cisplatin-resistant lung cancer cell line A549/DDP, compared with their parental cells, respectively (22). In the present study, the findings revealed that TCRP1 expression was increased in tamoxifen-resistant breast cancer tissues compared with the primary breast cancer tissues in vivo. Consistently, in vitro TCRP1 expression was also increased in tamoxifen-resistant breast cancer cell line MCF7-R compared with the primary breast cancer cell line MCF-7. These data revealed that TCRP1 may be a pivotal regulator in mediating tamoxifen resistance in MCF-7 cells.

MCF-7 cells are widely used in studying acquired tamoxifen resistance, since it is not only a stable breast cancer cell line, but it is also the most characterized cell line (2327). MCF7-R cells are characterized by activating another survival signal pathway to support breast cancer growth and inhibit the apoptotic-promoting effect of tamoxifen (23). In addition, TCRP1 plays a tumor-promoting role in carcinogenesis and cancer progression. Previous studies reported that overexpressed TCRP1 promoted cell proliferation, cell growth, and cell cycle progression in NIH/3T3 cells. Moreover, upregulated TCRP1 promoted tumorigenesis of NIH/3T3 cells in nude mice (17). It has been reported that TCRP1-proficient cells exhibit an improved survival chance by increasing the levels of antiapoptotic proteins and decreasing the level of proapoptotic proteins (15). However, the function of TCRP1 in tamoxifen-resistant breast cancer is still unknown. Thus, the role of TCRP1 in MCF7-R cells was investigated. The present results revealed that knockdown of TCRP1 decreased the resistance of MCF7-R cells to tamoxifen by inhibiting MCF7-R cell growth and promoting cell apoptosis.

Studies on the mechanisms of tamoxifen resistance demonstrated that tumor cells recruit various signaling pathways to develop drug resistance (28,29). It has been reported that TCRP1 promoted NIH/3T3 cell transformation by over-activating PDK1 and AKT1 (17). In addition, gene amplification or aberrant phosphorylation in the cytosol and nucleus may lead to aberrant PDK1 expression in human cancers such as in colon and breast cancer (3033). The results revealed that TCRP1 regulated the phosphorylation of PDK1. Specifically, the level of p-PDK1 was significantly increased after TCRP1 overexpression, which was contrary to the knockdown of TCRP1. Although the downstream of phosphorylated PDK1 after TCRP1 stimulation remained unclear, recently, some studies have revealed that PDK1/SGK1 signaling plays multiple roles in a variety of physiological processes such as cell growth, proliferation and survival (20) (3437). For instance, PDK1 activity was suppressed to inhibit downstream SGK1 phosphorylation and activity of BYL719-resistant cells (20). Hence, the correlations among tamoxifen resistance and PDK1 and SGK1 under TCRP1 stimulation were analyzed in the present study, and the present data indicated that TCRP1 led to tamoxifen resistance by regulating the phosphorylation of PDK1, which further activated SGK1.

It should also be noted that there are four limitations in the present study. Firstly, the correlation between TCRP1 and the prognosis of tamoxifen-resistant breast cancer patients was not investigated. Secondly, the effects of TCRP1 on various types of breast cancer cell lines should be studied, to confirm and render the present results more convincing. Thirdly, overexpressing TCRP1 in MCF-7 cells should be performed to observe the effect of TCRP1 on the sensitivity of the breast cancer cells to tamoxifen and the phosphorylation of SGK1. Finally, the clinical data of patients, including ER, PR, HER2 were not obtained in the tissues of patients.

In conclusion, the present data strongly support that TCRP1 contributes to tamoxifen resistance possibly through the activation of the PDK1/SGK1 signaling pathway in MCF7-R cells. The data provide new insights into chemoresistance and provide a novel therapeutic approach that may allow us to prevent tamoxifen resistance in breast cancer in the future.

Acknowledgements

Not applicable.

Funding

No funding was received.

Availiability of data and materials

The analyzed data sets generated during the study are available from the corresponding author on reasonable request.

Authors' contributions

SZ and XL performed the experiments and the data analysis. JL and XZ wrote the initial draft of the manuscript, conceived and designed the experiments. LY and LH contributed to the literature search, obtained the tumor and tissues with clinical information where it pertained. All authors revised the manuscript. All authors read and approved the final manuscript and agree to be accountable for all aspects of the research in ensuring that the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Ethics approval and consent to participate

The experimental protocol was approved by the Ethical Committee of Wuzhong People's Hospital, Suzhou, China. The breast cancer tissues were collected from Wuzhong People's Hospital with written informed consent and permission from the Institutional Review Board. All patients provided written informed consent. All animal studies were approved by the Animal Experimentation Ethics Committee of Wuzhong People's Hospital.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Glossary

Abbreviations

Abbreviations:

TCRP1

tongue cancer resistance-related protein 1

PDK1

phosphoinositide-dependent kinase 1

SGK1

glucocorticoid-inducible kinase 1

DMEM

Dulbecco's modified Eagle's medium

FBS

fetal bovine serum

cDNA

complementary DNA

ER

estrogen receptors

mRNA

messenger RNA

PVDF

polyvinylidene difluoride

shRNA

short hairpin RNA

GAPDH

glyceraldehyde-3-phosphate dehydrogenase

NSh

non-short hairpin

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June-2020
Volume 43 Issue 6

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Copy and paste a formatted citation
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Spandidos Publications style
Zhao S, Li X, Yin L, Hou L, Lan J and Zhu X: TCRP1 induces tamoxifen resistance by promoting the activation of SGK1 in MCF‑7 cells. Oncol Rep 43: 2017-2027, 2020
APA
Zhao, S., Li, X., Yin, L., Hou, L., Lan, J., & Zhu, X. (2020). TCRP1 induces tamoxifen resistance by promoting the activation of SGK1 in MCF‑7 cells. Oncology Reports, 43, 2017-2027. https://doi.org/10.3892/or.2020.7577
MLA
Zhao, S., Li, X., Yin, L., Hou, L., Lan, J., Zhu, X."TCRP1 induces tamoxifen resistance by promoting the activation of SGK1 in MCF‑7 cells". Oncology Reports 43.6 (2020): 2017-2027.
Chicago
Zhao, S., Li, X., Yin, L., Hou, L., Lan, J., Zhu, X."TCRP1 induces tamoxifen resistance by promoting the activation of SGK1 in MCF‑7 cells". Oncology Reports 43, no. 6 (2020): 2017-2027. https://doi.org/10.3892/or.2020.7577