Establishment and characterization of a new human extrahepatic bile duct carcinoma cell line (ICBD-1).

  • Authors:
    • I Takiyama
    • M Terashima
    • K Ikeda
    • H Kawamura
    • M Kashiwaba
    • G Tamura
    • T Suto
    • F Nakashima
    • R Sasaki
    • K Saito
  • View Affiliations

  • Published online on: March 1, 1998     https://doi.org/10.3892/or.5.2.463
  • Pages: 463-470
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Abstract

A new human extrahepatic bile duct carcinoma cell line (ICBD-1) was established from surgically resected tumor of a 71-year-old Japanese male patient. ICBD-1 cells proliferate in a layer with a population doubling time of 31.5 h and secrete tissue polypeptide antigen. ICBD-1 cells have a tetraploid pattern with a DNA index of 1.83 and chromosome counts showed equally distribution in a range from 65 to 69. IC50 values for ICBD-1 cells were 200 ng/ml for adriamycin, 400 ng/ml for mitomycin C, 2 microg/ml for cisplatin and 300 ng/ml for 5-fluorouracil. ICBD-1 cells were successfully transplanted to male nude mice, inducing progressive tumor growth. Histologically, nude mouse tumors were less differentiated than the original human tumor. Tumor cells showed alveolar structures with thin fibrous stroma, classified as poorly-differentiated adenocarcinoma. ICBD-1 is the fourth established cell line that originate from extrahepatic bile duct carcinoma and it will be applicable for the experimental studies of this disease.

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Mar-Apr 1998
Volume 5 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Takiyama I, Terashima M, Ikeda K, Kawamura H, Kashiwaba M, Tamura G, Suto T, Nakashima F, Sasaki R, Saito K, Saito K, et al: Establishment and characterization of a new human extrahepatic bile duct carcinoma cell line (ICBD-1).. Oncol Rep 5: 463-470, 1998
APA
Takiyama, I., Terashima, M., Ikeda, K., Kawamura, H., Kashiwaba, M., Tamura, G. ... Saito, K. (1998). Establishment and characterization of a new human extrahepatic bile duct carcinoma cell line (ICBD-1).. Oncology Reports, 5, 463-470. https://doi.org/10.3892/or.5.2.463
MLA
Takiyama, I., Terashima, M., Ikeda, K., Kawamura, H., Kashiwaba, M., Tamura, G., Suto, T., Nakashima, F., Sasaki, R., Saito, K."Establishment and characterization of a new human extrahepatic bile duct carcinoma cell line (ICBD-1).". Oncology Reports 5.2 (1998): 463-470.
Chicago
Takiyama, I., Terashima, M., Ikeda, K., Kawamura, H., Kashiwaba, M., Tamura, G., Suto, T., Nakashima, F., Sasaki, R., Saito, K."Establishment and characterization of a new human extrahepatic bile duct carcinoma cell line (ICBD-1).". Oncology Reports 5, no. 2 (1998): 463-470. https://doi.org/10.3892/or.5.2.463