BR Biomedical Reports 2049-9434 2049-9442 D.A. Spandidos 10.3892/br.2018.1159 BR-0-0-1159 Articles Association between serum 25-hydroxyvitamin D, hemoglobin A1c and fasting blood glucose levels in adults with diabetes mellitus AlkhatatbehMohammad J. Abdul-RazzakKhalid K. Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid 22110, Jordan Correspondence to: Dr Mohammad J. Alkhatatbeh, Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Amman-Ramtha Street, Irbid 22110, Jordan, E-mail: khatatbeh@just.edu.jo 12 2018 17 10 2018 9 6 523 530 24082018 16102018 Copyright © 2018, Spandidos Publications 2018

In the present study, the aim was to investigate the association between serum 25-hydroxyvitamin D concentration and measures of glycemic control including hemoglobin A1c (HbA1c) and fasting blood glucose (FBG) in adult patients with diabetes mellitus (DM) from the north of Jordan. Another aim was to compare serum levels of 25-hydroxyvitamin D between patients with good glycemic control and patients with uncontrolled DM. This was a cross-sectional study that included 261 participants with DM. The concentration of 25-hydroxyvitamin D was measured using electrochemiluminescence immunoassay, HbA1c was measured using turbidimetric inhibition immunoassay and FBG was measured using the hexokinase method. Data regarding other clinical variables were obtained from medical records or by self-reporting. Participants with good glycemic control exhibited significantly higher levels of 25-hydroxyvitamin D compared with participants with uncontrolled DM (P=0.03). Participants with sufficient vitamin D status (>30 ng/ml in serum) exhibited significantly lower HbA1c level compared with participants with deficient vitamin D (<20 ng/ml) status (P=0.02). Correlation analysis determined significant inverse correlations between 25-hydroxyvitamin D levels and HbA1c and FBG levels (r=−0.23 and −0.17, respectively, both P<0.01). There were also significant correlations between duration of DM and HbA1c and FBG levels (both r=0.21, P<0.01). HbA1c level was also inversely correlated with participants' age (r=−0.19, P<0.01). Further multiple linear regression analysis revealed an inverse significant association between HbA1c and 25-hydroxyvitamin D levels (F=12.95, R2=0.48, P<0.01) but did not identify a similar association between FBG and 25-hydroxyvitamin D levels. These findings may encourage further research to identify if vitamin D supplementation may improve measures of glycemic control, and how vitamin D may affect glucose homeostasis in patients with DM.

 vitamin D diabetes mellitus hemoglobin A1c glycemic control Introduction

Diabetes mellitus (DM) is characterized by chronic hyperglycemia caused by impaired insulin secretion, peripheral insulin resistance, or both (1). To prevent long-term micro- and macro-vascular complications, patients with DM are required to maintain adequate glycemic control, which is routinely assessed by measuring hemoglobin A1c (HbA1c) and fasting blood glucose (FBG) levels (2). This therapeutic target can be achieved by adhering to appropriate dietary and lifestyle modifications and to the proper medications (3).

Although nutrition recommendations for patients with DM focus on the proportion of calories obtained from carbohydrate, fat and protein (4), there is increasing data suggesting that vitamin D supplementation may be associated with improved glycemic control (5–7). This was based on prior studies that determined significant inverse associations between serum vitamin D level and measures of glycemic control including both HbA1c and FBG levels (8–10). The involvement of vitamin D in glucose metabolism is considered be associated with its role in pancreatic insulin secretion and peripheral insulin resistance (11). Despite being classified as a vitamin, vitamin D has also been noted to serve several hormonal functions that are proposed to result from its action on vitamin D receptors (VDRs), which are widely expressed on various cell types (12). Among these functions is the action of vitamin D on the VDRs of pancreatic beta islet cells (12). In animal studies, mice lacking functional VDRs exhibited impaired insulin secretion (13); furthermore, vitamin D supplementation was able to induce insulin biosynthesis in the pancreatic islets of rats (14). In humans, certain VDR gene variants have been associated with impaired insulin secretion and the development of type 2 DM (15). In addition, vitamin D may serve a role in peripheral insulin sensitivity through its action on VDRs expressed on human skeletal muscle and adipose tissue cells (11,16,17). These cells are involved in determining peripheral insulin sensitivity as they are responsible for glucose uptake in response to insulin secretion (11).

Despite reports of a possible role for vitamin D in DM (5,8,9), to our knowledge the present study is the first to investigate the association between serum vitamin D, HbA1c and FBG levels in adults with DM from northern Jordan. The aim was to compare serum levels of 25-hydroxyvitamin D between DM patients with good glycemic control and patients with uncontrolled DM. Additionally, it was investigated whether associations existed between measures of glycemic control and other variables including age, duration of DM and medications.

 Materials and methods <sec> <title>Study design

The current study was a cross-sectional study that involved 261 male and female adults (aged 19–79 years old; 111 men and 150 women) with type 1 or type 2 DM. This represented a response rate of 87% following assessment of eligibility. Patients were recruited from the outpatient diabetes clinic of King Abdullah University Hospital, Ramtha, Jordan between December 2016 and January 2018. Patients with chronic renal failure, chronic liver disease and/or on vitamin D supplementation for the past 3 months were excluded from the study. All participants were taking medications for the treatment of DM and its complications. Participants had been informed of the purpose of the study prior to signing consent forms. The study procedure was ethically approved by the Institutional Research Board of Jordan University of Science and Technology and King Abdullah University Hospital (Irbid, Jordan; approval no. 1092015).

 Sample size calculation

Sample size was calculated based on the reported prevalence of vitamin D deficiency and insufficiency among patients with DM in northern Jordan, which was 87.1% as determined in our previous study (18). Sample size was determined using the formula [sample size= (t)2(p)(q)/(d)2] (19), where t-value (t=1.96) represents the 95% confidence interval, p=0.87 [the estimated prevalence of vitamin D deficiency and insufficiency (18)], q=1-p and d=0.05 (the margin of error based on the 95% confidence interval). Consequently, the calculated sample size was 173 patients. The current study included a larger number of patients with DM to increase reliability of results.

 Data collection

Descriptive information regarding age, sex, type of DM, duration of DM, smoking status, history of chronic renal failure, history of chronic liver disease, history of previous supplementation of vitamin D and list of current medications were obtained from patients' medical records and/or by self-reporting. Body weight (kg) was measured using a calibrated balance with wearing of light clothes and without shoes. Height (cm) and waist circumference (cm) were measured using a metric scale tape and body mass index (BMI) was calculated by dividing weight (kg) by height squared (m2). Systolic and diastolic blood pressures (SBP and DBP) were measured using a mercury sphygmomanometer by a registered nurse.

 Blood processing and laboratory assays

Following overnight fasting, 10-ml venous blood samples were collected by a qualified laboratory technician to determine 25-hydroxyvitamin D, HbA1c and FBG levels. Serum was prepared within 2 h of blood collection by centrifuging blood samples at 2,100 × g for 8 min using a high-speed Jouan MR23i centrifuge (Thermo Fisher Scientific, Inc., Waltham, MA, USA), with maintenance and preparation all at room temperature. FBG concentration was determined by the hexokinase method (20) using a Hitachi 902 auto-analyzer (Roche Diagnostics GmbH, Mannheim, Germany). The level of HbA1c was determined by turbidimetric inhibition immunoassay (21) using a cobas b 101 system (Roche Diagnostics GmbH). According to a recent report from the American College of Physicians that recommended a target HbA1c of 7–8% for adults with DM (2), participants with HbA1c <8% were considered as having good glycemic control while participants with HbA1c ≥8% were considered as having uncontrolled DM. Serum 25-hydroxyvitamin D concentration was determined by electrochemiluminescence immunoassay (22) using a Roche Modular E170 Analyzer (Roche Diagnostics GmbH). Participants with serum 25-hydroxyvitamin D >30 ng/ml were considered as having sufficient vitamin D levels whereas participants with serum 25-hydroxyvitamin D of 20–30 ng/ml or less than 20 ng/ml were considered as having insufficient or deficient vitamin D levels, respectively (23).

 Statistical analysis

The IBM SPSS statistics 20.0 software (IBM Corp., Armonk, New York, USA) was used to perform statistical analysis. All non-normally distributed continuous variables were log-transformed prior to analysis. Continuous variables were presented as mean ± standard deviation or median (interquartile range). Qualitative variables were presented as number (percentage). Differences in the mean levels of continuous variables between participants with good glycemic control and participants with uncontrolled DM were determined by Student's t-test. Differences in qualitative variables between participants with good glycemic control and participants with uncontrolled DM were determined by χ2 test. Differences in the mean level of 25-hydroxyvitamin D between participants with deficient, insufficient and sufficient vitamin D levels were determined using one-way analysis of variance with Tukey's post hoc test for multiple comparisons. Correlation analyses between HbA1c, FBG and other continuous variables were performed using the Pearson product-moment correlation test. Multiple linear regression analysis was used to detect independent predictors of HbA1c and FBG. All P-values were two-sided and considered statistically significant at <0.05.

 Results <sec> <title>Characteristics of participants

The mean age of the patients was 54.94±10.92 years. The mean duration of DM was 7.84±6.58 years, the mean BMI was 30.77±4.80 kg/m2, the mean waist circumference was 103.68±12.91 cm, the mean SBP was 137.02±17.05 mmHg and the mean DBP was 82.65±10.51 mmHg. The median fasting blood glucose (FBG) was 8.60 (6.60–12.53) mmol/l, the median HbA1c was 8.00 (6.88–9.83) % and the median serum 25-hydroxyvitamin D concentration was 14.80 (8.42–22.67) ng/ml. A total of 52 (19.9%) participants were current smokers and 209 (80.1%) participants were non-smokers. A total of 204 (78.2%) participants were on insulin therapy, 238 (91.2%) participants were on metformin therapy, 96 (36.8%) participants were on sulfonylureas therapy, 98 (37.5%) participants were on aspirin therapy, 123 (47.1%) participants were on statins therapy, 39 (14.9%) participants were on beta blockers therapy, 79 (30.3%) participants were on angiotensin converting enzyme inhibitors therapy, 32 (12.3%) participants were on calcium channel blockers therapy and 45 (17.2%) participants were on proton pump inhibitors therapy. Descriptive characteristics of the participants according to their glycemic control are presented in Table I.

 Correlation of HbA1c and FBG with serum 25-hydroxyvitamin D and other variables

As shown in Table I, serum 25-hydroxyvitamin D was significantly higher in participants with controlled DM compared with in those with uncontrolled DM (P=0.03). HbA1c level was significantly higher in participants with deficient (<20 ng/ml) vitamin D level compared with in participants with sufficient (>30 ng/ml) vitamin D level (P=0.02; Fig. 1A). Whereas, there was no significant difference in HbA1c level between participants with deficient or sufficient and insufficient vitamin D levels (P>0.05; Fig. 1A). In addition, there was no significant difference in FBG level between participants with sufficient, insufficient and deficient vitamin D levels (Fig. 1A). Further correlation analyses (Table II) between HbA1c, FBG, serum 25-hydroxyvitamin D and other variables determined significant inverse correlations between serum 25-hydroxyvitamin D and HbA1c (r=−0.23, P<0.01) and between serum 25-hydroxyvitamin D and FBG (r=−0.17, P<0.01; Fig. 1B). HbA1c was also inversely correlated with participant's age (r=−0.19, P<0.01) and directly correlated with duration of DM (r=0.21, P<0.01) and FBG (r=0.61, P<0.01). FBG was also directly correlated with duration of DM (r=0.21, P<0.01).

 Association of HbA1c and FBG with serum 25-hydroxyvitamin D and other variables

Multiple linear regression analyses were performed to identify the potential predictors of HbA1c and FBG levels (Table III). HbA1c was inversely associated with serum 25-hydroxyvitamin D level and directly associated with FBG (both P<0.01). HbA1c was also associated with type of DM (P<0.01), indicating that participants with type 1 DM had higher levels of HbA1c (Tables I and III). FBG was associated with statins therapy (P=0.04), indicating that participants on statin therapy had lower levels of FBG (Tables I and III).

 Discussion

In the present descriptive study, the aim was to assess the association between serum 25-hydroxyvitamin D levels and measures of glycemic control in adults with DM from the north of Jordan. Patients with good glycemic control exhibited significantly higher levels of 25-hydroxyvitamin D compared with patients with uncontrolled DM. Additionally, patients with sufficient vitamin D status exhibited significantly lower HbA1c levels (but not FBG levels) compared with patients with deficient vitamin D status. Correlation analysis also revealed significant inverse correlations between 25-hydroxyvitamin D levels and HbA1c and FBG levels. Further multiple linear regression analysis identified an inverse significant association between HbA1c and 25-hydroxyvitamin D levels but did not find similar association between FBG and 25-hydroxyvitamin D levels.

These findings are comparable with those reported in previous similar studies. For instance, Zoppini et al (8) and Kostoglou-Athanassiou et al (10) observed a significant inverse association between 25-hydroxyvitamin D and HbA1c levels in patients with type 2 DM, though Zoppini et al (8) did not detect a significant correlation between 25-hydroxyvitamin D and FBG levels in their cohort. In addition, Lim et al (24) and Kajbaf et al (25) found a significant inverse association between 25-hydroxyvitamin D and HbA1c levels in type 2 DM patients with chronic kidney disease. In patients with type 1 or 2 DM, Buhary et al (5) also detected a significant inverse association between HbA1c and 25-hydroxyvitamin D levels, and observed that supplementation of vitamin D was able to improve glycemic control by reducing HbA1c levels. Although this finding was supported by a recent systematic review and meta-analysis, which concluded that supplementation of vitamin D was associated with reduced HbA1c levels in patients with type 2 DM (7), other systematic reviews and meta-analyses (26,27) have not supported the notion that vitamin D supplementation may improve measures of glycemic control including HbA1c levels. Thus, the association between vitamin D and HbA1c levels in patients with DM does not essentially imply the involvement of vitamin D supplementation in improving glycemic control. This exposes the question of whether the relationship between vitamin D deficiency and glucose homeostasis is causal or confounding (28).

However, the possible role of vitamin D in glucose metabolism may be due to its action on VDRs expressed on cells of the pancreatic beta islets (12), skeletal muscle and adipose tissue (11,16,17). Vitamin D binding to these receptors may be involved in enhancing pancreatic insulin secretion and peripheral insulin sensitivity by increasing glucose uptake in skeletal muscle and adipose tissue (11). The inconsistent effect of vitamin D supplementation in improving glycemic control (5,7,26,27) may be explained by the possible action of vitamin D on enhancing insulin secretion, which depends on the reserved function of the pancreatic beta islet cells (29). Patients with type 2 DM may have variable and progressive decrease in pancreatic insulin secretion (29). Therefore, the variability in responding to vitamin D supplementation could be due to the variability in the reserved beta islet cell function, which may differ from one patient to another. Consequently, it is possible that if patients have complete pancreatic beta cell dysfunction, vitamin D supplementation may not affect insulin secretion and thus glycemic control.

In the current study, 25-hydroxyvitamin D was inversely associated with HbA1c but not with FBG. This suggests that maintaining a sufficient vitamin D level in DM patients may improve HbA1c but not FBG. The difference between the two glycemic measures is that HbA1c reflects the average level of blood glucose over the past 2–3 months while FBG represents a single measurement of blood glucose concentration following overnight fasting (30). As FBG is determined by the level of basal insulin secretion and HbA1c by the level of both basal and postprandial insulin secretion (31), the present results suggest that the association between vitamin D and HbA1c could be due to vitamin D involvement in postprandial insulin secretion rather than basal insulin secretion. The effect of vitamin D on insulin secretion may also be indirect by increasing intracellular calcium (28). One of the principal functions of vitamin D is to increase calcium absorption from the gastrointestinal tract (32). In the postprandial situation, increased vitamin D-dependent calcium absorption may increase intracellular calcium, which could act as a mediator of postprandial insulin secretion (28) and thus improve HbA1c level without affecting FBG level.

Although participants with controlled DM were of significantly increased age, presented with shorter duration of DM, were of a greater female proportion and were using metformin more than participants with uncontrolled DM, multiple linear regression analysis did not reveal any significant association between these parameters and measures of glycemic control. Participants with type 2 DM more frequently had controlled DM compared with participants with type 1 DM. Consistently, regression analysis demonstrated a significant association between HbA1c level and DM type, which suggests that patients with type 1 DM had higher levels of HbA1c compared with patients with type 2 DM. Interestingly, FBG level was significantly inversely associated with the use of statin therapy, suggesting that these medications may have a positive effect on FBG.

Collectively, the current study confirmed a significant inverse association between serum 25-hydroxyvitamin D and HbA1c levels but did not identify such association between 25-hydroxyvitamin D and FBG levels in adult patients with DM from northern Jordan. The current study had strength in its relatively large sample size, making the results comparable with findings reported in other similar studies. In addition, the impact of patients' medications on the association between 25-hydroxyvitamin D and glycemic control was taken in consideration by including these medications in the regression analysis. However, the cross-sectional design prevented investigation into why 25-hydroxyvitamin D level was associated with HbA1c. As such, this association requires further investigation to determine if vitamin D supplementation may improve glycemic control in adults with DM from Jordan. If vitamin D supplementation was to improve glycemic control, then further research to determine the mechanism by which vitamin D affects glucose homeostasis would be warranted. Another limitation was the emphasis on detecting an association between serum 25-hydroxyvitamin D level and measures of glycemic control without investigating a possible association between serum 25-hydroxyvitamin D and DM complications, which are the most important factors affecting the prognosis of DM patients (33). However, this may encourage future studies into the potential relationship between serum vitamin D and individual DM complications such as eye lesions, vascular lesions and kidney disease.

In conclusion, the present study observed a significant inverse association between serum 25-hydroxyvitamin D and HbA1c levels in adult patients with DM. Serum 25-hydroxyvitamin D level in patients with good glycemic control was significantly higher than in patients with uncontrolled DM. These findings may enhance further research to identify if vitamin D supplementation can improve HbA1c level in patients with DM, and if vitamin D can affect glucose homeostasis in DM.

 Acknowledgements

Not applicable.

 Funding

The current study was supported by the Deanship of Research at Jordan University of Science and Technology (grant no. 109/2015).

 Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

 Authors' contributions

MJA was responsible for study design, patient recruitment, data collection, data analysis and manuscript writing. KKAR was responsible for data interpretation and manuscript editing. Both authors approved the final version of the manuscript to be published.

 Ethics approval and consent to participate

The study procedure was ethically approved by the Institutional Research Board of Jordan University of Science and Technology and King Abdullah University Hospital (Irbid, Jordan; approval no. 1092015). All participants agreed to participate in this study by signed written informed consent.

 Patient consent for publication

All participants provided written informed consent permitting publication of relevant data following anonymization of personal information.

 Competing interests

The authors declare that they have no competing interests.

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Association between serum 25-hydroxyvitamin D, HbA1c and FBG levels in adults with diabetes mellitus. (A) HbA1c and FBG levels in participants according to vitamin D status. HbA1c level in participants with sufficient vitamin D (>30 ng/ml) was significantly higher than the level in participants with deficient vitamin D (<20 ng/ml). There was no significant difference in HbA1c level between participants with insufficient vitamin D (20–30 ng/ml) and participants with either sufficient or deficient vitamin D. In addition, there was no significant difference in FBG level between participants with sufficient, insufficient or deficient vitamin D. Data is expressed as mean ± standard deviation. (B) Significant correlation between HbA1c and FBG levels and significant inverse correlations between 25-hydroxyvitamin D and HbA1c and FBG levels. *P=0.02. HbA1c, hemoglobin A1c; FBG, fasting blood glucose.

Characteristics of participants according to level of glycemic control.

Characteristics Good glycemic control, HbA1c <8% (n=129) Glycemic uncontrolled, HbA1c ≥8% (n=132) P-valuea
Age, years 56.72±9.86 53.19±11.64 <0.01
Sex <0.01
  Male 43 (33.3) 68 (51.5)
  Female 86 (66.7) 64 (48.5)
Smoking 21 (16.3) 31 (23.5) 0.16
Height, cm 165.28±9.07 167.07±10.19 0.14
Weight, kg 85.43±13.00 84.33±15.30 0.53
BMI, kg/m2 31.16±4.12 30.38±5.37 0.19
WC, cm 104.18±11.93 103.20±13.82 0.55
DM <0.01
  Type 1 2 (1.6) 20 (15.2)
  Type 2 127 (98.4) 112 (84.8)
Log [duration of DM (months)] 1.67±0.49 1.87±0.51 <0.01
Log [FBG (mmol/l)] 0.87±0.14 1.06±0.19 <0.01
Log [HbA1c (%)] 0.83±0.06 1.00±0.07 <0.01
SBP, mmHg 136.58±16.08 137.45±18.01 0.68
DBP, mmHg 83.61±9.22 81.70±11.60 0.15
Insulin 100 (77.5) 104 (78.8) 0.80
Metformin 124 (96.1) 114 (86.4) <0.01
Sulfonylureas 44 (34.1) 52 (39.4) 0.38
Aspirin 53 (41.1) 45 (34.1) 0.24
Statins 65 (50.4) 58 (43.9) 0.30
Beta blockers 19 (14.7) 20 (15.2) 0.92
ACEIs 39 (30.2) 40 (30.3) 0.99
CCBs 18 (14.0) 14 (10.6) 0.41
PPIs 20 (15.5) 25 (18.9) 0.46
Log [25-hydroxyvitamin D (ng/ml)] 1.18±0.26 1.10±0.30 0.03

Student's t-test or χ2 test (P-values were two-tailed and considered statistically significant at <0.05). Data were expressed as mean ± standard deviation or number of participants (%). HbA1c, hemoglobin A1c; DM, diabetes mellitus; BMI, body mass index; WC, waist circumference; FBG, fasting blood glucose; SBP, systolic blood pressure; DBP, diastolic blood pressure; ACEIs, angiotensin converting enzyme inhibitors; CCBs, calcium channel blockers; PPIs, proton pump inhibitors.

Correlation of HbA1c and FBG with other variables.

Log [HbA1c (%)] Log [FBG (mmol/l)]
Characteristics r P-valuea r P-valuea
Age −0.19 <0.01 −0.12 0.06
Height, cm 0.04 0.50 −0.05 0.45
Weight, kg −0.08 0.21 0.02 0.75
BMI, kg/m2 −0.08 0.21 0.05 0.42
WC, cm −0.03 0.63 0.11 0.10
Log [duration of DM (months)] 0.21 <0.01 0.21 <0.01
Log [FBG (mmol/l)] 0.61 <0.01 – –
SBP (mmHg) 0.05 0.46 0.01 0.87
DBP (mmHg) −0.10 0.12 −0.07 0.25
Log [25-hydroxyvitamin D (ng/ml)] −0.23 <0.01 −0.17 <0.01

Pearson product-moment correlation test (P-values were two-tailed and considered statistically significant at <0.05). r, correlation coefficient; HbA1c, hemoglobin A1c; DM, diabetes mellitus; BMI, body mass index; WC, waist circumference; FBG, fasting blood glucose; SBP, systolic blood pressure; DBP, diastolic blood pressure.

Association of HbA1c and FBG with other variables.

Dependent variable R2 ANOVA Model B β t-value P-valuea
Log [HbA1c (%)] 0.48 F=12.95, P<0.01 Constant 0.86 – 13.24 <0.01
Age <-0.01 −0.06 −1.03 0.30
Log (duration of DM) 0.02 0.08 1.47 0.14
Log [FBG (mmol/l)] 0.29 0.54 10.53 <0.01
Log [25-hydroxy- vitamin D (ng/ml)] −0.05 −0.13 −2.67 <0.01
Type of DM −0.06 −0.17 −2.39 0.02
Sex −0.02 −0.09 −1.64 0.10
Smoking 0.01 0.03 0.56 0.57
Metformin therapy −0.03 −0.07 −1.17 0.24
Sulfonylureas therapy 0.01 0.03 0.56 0.58
Insulin therapy <0.01 <0.01 0.15 0.88
Statin therapy <0.01 0.04 0.70 0.49
Beta blocker therapy 0.02 0.06 1.15 0.25
ACEIs therapy 0.02 0.07 1.18 0.24
Aspirin therapy −0.02 −0.11 −1.81 0.07
CCBs therapy <-0.01 −0.01 −0.27 0.79
PPIs therapy −0.01 −0.05 −1.06 0.29
Log [FBG (mmol/l)] 0.42 F=11.00, P<0.01 Constant −0.15 – −0.86 0.39
Log (duration of DM) 0.03 0.07 1.29 0.20
Log [HbA1c (%)] 1.14 0.61 10.69 <0.01
Log [25-hydroxy-vitamin D (ng/ml)] −0.02 −0.04 −0.68 0.50
Type of DM −0.02 −0.03 −0.41 0.68
Sex <0.01 0.02 0.27 0.79
Smoking <0.01 <0.01 0.13 0.90
Metformin therapy 0.09 0.13 2.01 0.05
Sulfonylureas therapy 0.02 0.06 1.02 0.31
Insulin therapy −0.02 −0.05 −0.88 0.38
Statins therapy −0.05 −0.12 −2.02 0.04
Beta blocker therapy −0.01 −0.02 −0.33 0.74
ACEIs therapy −0.03 −0.06 −1.07 0.29
Aspirin therapy 0.04 0.11 1.75 0.08
CCBs therapy <0.01 0.01 0.24 0.81
PPIs therapy 0.03 0.06 1.03 0.30

Multiple linear regression analysis (P-values were considered statistically significant at <0.05). HbA1c, hemoglobin A1c; DM, diabetes mellitus; FBG, fasting blood glucose; ACEIs, angiotensin converting enzyme inhibitors; CCBs, calcium channel blockers; PPIs, proton pump inhibitors; R2, coefficient of determination; B, unstandardized coefficient; β, standardized coefficient; F, F-statistic; t, t-statistic.