Introduction
Diabetes is a severe disorder since it strongly increases the risk of cardiovascular complications, such as coronary artery disease, myocardial infarction, hypertension and dyslipidemia (1,2). The cardiovascular injury mainly targets two important organs, eyes and kidneys (3). This has made diabetic nephropathy (DN) one of the most common complications for diabetic patients (4,5). The main typical renal histological changes of DN are caused by the changes in the extracellular matrix (ECM). The ECM accumulation in DN results in mesangial expansion, tubulointerstitial fibrosis, and irreversible deterioration of renal function (6,7).
It is reported that transforming growth factor-β1 (TGF-β1) plays an important role in the development of DN (8–10). After binding to its receptor, TGF-β1 activates two critical downstream mediators, Smad2 and Smad3, to exert its biological activities such as ECM production, which is negatively regulated by Smad7, an inhibitor Smad. The effect of TGF-β1/Smad3 signaling pathway on mediating renal fibrosis has been well recognized (11,12), suggesting that the block of TGF-β1/Smad3 can provide an effective approach for preventing the progression of DN.
However, no proper effective treatment has been detected so far for retarding the progression of renal failure. Therefore, new therapeutic strategies in the management of DN are required (13).
Sitagliptin (SIT), a dipeptidyl peptidase-4 (DPP-4) inhibitor, exhibited a modest beneficial effect on glycated hemoglobin levels when used in combination with metformin for treatment of diabetes mellitus type 2 (14). Recent studies have shown that SIT alone can offer cardiovascular and neuropathic protections which are probably mediated through antioxidant, anti-inflammatory, and anti-apoptotic mechanisms (15–21). It is reported that SIT can also ameliorate renal ischemia reperfusion injury in rats (22). However, whether SIT has beneficial effect on prevention of DN remains unknown.
In this study the therapeutic effect of SIT for prevention of DN was systematically verified. Moreover, the possible mechanism through TGF-β1/Smad signaling was investigated for the first time.
Materials and methods
Ethics statements
This study was carried out in strict accordance with the recommendations in the Guiding Principles for Care and Use of Laboratory Animals of Xuzhou Medical University. The protocol was approved by the Committee on the Ethics of Laboratory Animals of Xuzhou Medical University (Xuzhou, China). All surgery was performed under sodium pentobarbital anesthesia, and all efforts were made to minimize suffering.
Animal experiments
Male Sprague-Dawley rats (certificate no. SYXK2010-0011), weighing 180–200 g were obtained from the Laboratory Animal Center of Xuzhou Medical University (Xuzhou, China). The animals were housed in standard metabolic cages and maintained under standard condition at constant room temperature of 20–25°C and humidity of 40–70% with a 12:12 h light and dark cycle. All rats had free access to commercial regular chow and water for one week before the dietary manipulation. After one week's adaptation, fasting blood glucose (FBG) values were measured by OneTouch II blood glucose meter with overnight fasting. Thirty rats were randomly divided into two groups: control group and study group. The control group was fed with regular chow and the study group was given a high fat diet (HFD) and high calorie diet (36.3% fat, 45% carbohydrate and 18.7% protein) for four weeks. Thereafter the values of FBG were calculated after 12 h fasting. The study group rats were initially injected intraperitoneally (i.p.) with streptozotocin (Sigma, St. Louis, MO, USA) at a low dose of 30 mg/kg diluted in citrate buffer (pH 4.4) (23–25), while the negative control ones (NC group) were injected with citrate buffer. One week later, the study group rats with an FBG <7.8 mmol/l were injected with repeated low dose of STZ (30 mg/kg). After four weeks of STZ injection, the study group rats with an FBG of ≥7.8 mmol/l twice or with random of ≥11.1 mmol/l were considered as type 2 diabetic rats (26). The type 2 diabetic rats were randomly divided into 2 groups: DN group (n=9) was treated daily with normal saline solution and SIT group (n=8) was administrated with SIT (Meck Sharp and Dohme Pty Ltd., Macquarie Park, NSW, Australia) at dose of 10 mg/kg (27). The same volume of normal saline solution was administered to the negative control group (NC group, n=9). The study period lasted 12 weeks.
Sample collection
After 12 weeks of treatment, the urine and blood samples were collected from the rats. After the animals were sacrificed, their kidneys were removed and weighed. The fresh kidney cortices were stored in formaldehyde solution for measurements. The kidney was fixed in 4% paraformaldehyde and the rest kidney tissues were snap frozen in liquid nitrogen and then stored at −80°C for later analysis.
Biochemical analysis
The values of urine protein were determined by using urinary protein kits from NanJing JianCheng Bioengineering Institute (Nanjing, China). The values of creatinine and blood urea nitrogen (BUN) were determined by enzyme-linked immunosorbent assay (ELISA) kits from Wuhan ColorfulGene Biological Technology Co., Ltd. (Wuhan, China). The kidney index was calculated by ratio of kidney weight (g)/body weight (kg).
Renal histological examination
After being fixed in 4% paraformaldehyde and embedded in paraffin, the kidney tissues were cut into 4-µm sections and stained with hematoxylin and eosin (H&E), periodic acid schiff (PAS), and Masson staining as previously described (28). Briefly, for Masson staining, the 4-µm section samples were firstly deparaffinized in water and then stained with Masson composite staining solution for 5 min, washed successively with 0.2% acetic acid solution, 5% phosphotungstic acid, 0.2% acetic acid solution, and finally stained with bright green staining solution for 5 min, washed twice with 0.2% acetic acid solution, dehydrated in absolute alcohol, put in xylene for transparency, and mounted with neutral gum. After this staining procedure, the collagen fibers were bluish green in color.
Immunohistochemistry examination
The immunohisto-chemistry analysis was performed according to the procedure reported previously (29). After the deparaffinization and hydration procedure, the 4-µm section samples were then washed with Tris-buffered saline (TBS; 10 mmol/l Tris HCl, 0.85% NaCl, pH 7.2). Endogenous peroxidase activity was quenched by incubating the section samples in 3% H2O2 deionized water. After overnight incubation with rabbit polyclonal anti-collagen IV antibody and rabbit monoclonal anti-fibronectin antibody (both from Abcam, Cambridge, MA, USA) at 4°C, the section samples were washed with TBS. Polymer helper was then added into the samples, and the section samples were incubated at room temperature for another 10 min. The slides were thereafter washed with TBS, incubated with poly-HRP anti-rabbit IgG at room temperature for final 10 min. A negative control without primary antibody was included in the experiment to verify antibody specificity. Finally, the sections were counterstained with 3,3′-diamino-benzidine (DAB) for 2 min. Brownish yellow granular or linear deposits were interpreted as positive areas. Accumulation of collagen IV and fibronectin was determined using the quantitative Image Analysis system. After ten random fields in both glomeruli and tubulointerstitium under light microscopy (×400) were outlined and positive staining patterns were identified, the percentage of positive area in the examined field was then determined. The arterial lumen space was excluded from the study. Data were expressed as percentage of positive area examined.
Reverse transcription-polymerase chain reaction (RT-PCR)
A RT-PCR procedure was performed to determine the relative mRNA quantities of TGF-β1 and Smad7. Total RNA was extracted from rat kidney cortices by using TRIzol reagent (Tiangen, Beijing, China) according to the manufacturer's instructions. The obtained total RNA was converted into cDNA using the TIANScript RT kit (Tiangen). The upsteam and downstrean primers (Shanghai Sangon Co., Shanghai, China) of these genes are shown in Table I. Levels of mRNA expression were subjected to housekeeping gene β-actin.
Western blotting
As described in previous studies (30), renal cortical tissues were rapidly dissected and homogenized in lysis buffer containing protease inhibitor cocktail (MedChem Express, Princeton, NJ, USA). After centrifugation at 12,000 × g for 10 min, total protein was collected. The protein concentration was determined by using the BCA protein assay kit (Beyotime Institute of Biotechnology, Shanghai, China) following the manufacturer's protocol. The protein samples were then mixed with sodium dodecyl sulfate-poly-acrylamide gel electrophoresis (SDS-PAGE) loading buffer (Beyotime Institute of Biotechnology) and boiled for 10 min. The protein samples were separated by 10% SDS polyacrylamide gel, and then transferred to nitrocellulose membranes. The transferred membranes were then blocked with blocking buffer (Beyotime Institute of Biotechnology) for 1 h at room temperature. Transferred membranes were incubated at 4°C overnight with rabbit polyclonal anti-TGF-β1 antibody, rabbit monoclonal anti-Smad3 antibody, rabbit monoclonal anti-p-Smad3 antibody, rabbit monoclonal anti-Smad7 antibody, rabbit monoclonal anti-GAPDH antibody (all from Abcam). The antigen-antibody complexes were washed three times for 5 min each and incubated with IRDye® 800CW goat anti-rabbit for 1 h at room temperature. After further three washes, the bands were added to infrared imaging system (Odyssey Sa; Li-COR, Lincoln, NE, USA). The intensity of TGF-β1 and Smad7 were subjected to GAPDH protein analysis while Smad3 activation was assessed by the ratio of phosphorylated form among its corresponding total protein levels.
Cell experiments
Rat mesangial cells (MCs), cultured in Dulbecco's modified Eagle's medium, and were incubated at 37°C in a humidified atmosphere of 5% CO2. During the experiments, the cells were first exposed to a normal concentration of glucose (NC, 5.56 mmol/l) for 24 h, and then retreated with high glucose (HG, 30 mmol/l glucose), high glucose with 0.1 µmol/ml sitagliptin (SITL), high glucose with 1 µmol/ml sitagliptin (SITM), and high glucose with 10 µmol/ml sitagliptin (SITH). Sitagliptin was dissolved in 1‰ (v/v) dimethyl sulfoxide (DMSO) and DMSO group was adopted as a control to rule out the effect of vehicle. Mannitol (MA) was adopted as a control to rule out the effect of osmotic pressure. The cells were harvested for analysis after 24 h.
Immunofluorescence
MCs were seeded into 12 well-plates with glass bottom inserts. Then the normal glucose medium of the 12 well-plates was replaced with various concentrations of sitagliptin or other controlling medium. After 24 h, cells were washed with phosphate-buffered saline (PBS) and fixed with cold polysorbate for 15 min at room temperature. After being washed intensively three times with PBS, the cells were added with 0.1% Triton X-100 in PBS for 15 min and blocked with 5% BSA in PBS buffer for 30 min at room temperature, and incubated with TGF-β1 and p-Smad3 antibodies at 4°C overnight. The cells were then incubated with DyLight 594 AffiniPure Donkey anti-rabbit IgG (H + L) 1 h at room temperature. The nuclei were counter-stained by 4,6-diamidino-2 phenylindole (DAPI) for 2 min, then washed again with PBS two times before mounting with fluorescence mounting medium. Images were captured by Olympus BX43F fluorescence microscope (Olympus, Tokyo, Japan).
Enzyme-linked immunosorbent assay
The levels of Smad7 protein in the MCs were determined by ELISA (R.B. Scientific, Waltham, MA, USA). The levels of Smad7 was determined according to the manufacturer's instructions. The colorimetric reaction was measured at 450 nm.
Statistical analysis
Data were expressed as means ± SE. Statistical analyses were performed using the paired t-test for two data comparison and one-way analysis of variance (ANOVA) for multiple data comparison. A value of P<0.05 was considered statistically significant.
Results
Biochemical analysis
As shown in Fig. 1, the kidney index and levels of FBG, 24 h urinary protein, BUN and Cr for the DN group were significantly higher than those of the NC group (P<0.01), suggesting that our early DN model was successful. The SIT group showed remarkably reduced kidney index and levels of 24 h urinary protein (P<0.05), BUN (P<0.01), and Cr (P<0.01), suggesting that SIT can improve the function of DN.
Histological and immunohistochemistry analysis
The H&E staining (Fig. 2A) and PAS staining (Fig. 2B) of the kidney sections from the control group revealed a normal glomerulus surrounded by Bowman's capsule and proximal and distal convoluted tubules without any inflammatory changes. The DN group showed renal tubule atrophy, thickening of the basement membrane. The SIT group exhibited a significantly-attenuated basement membrane. Compared with control group, the DN group exhibited a maximum accumulation of collagen IV (Col IV) and fibronectin (FN) in the renal cortex revealed by Masson staining (Fig. 2C) and immunohistochemistry (Fig. 2D–E). Compared with the DN group, significantly-reduced renal fibrosis was observed in the SIT-treated group. All of these fibrotic changes in the diabetic kidney were largely attenuated by treatment with SIT as indicated in Fig. 2F (P<0.01).
Effects of sitagliptin on TGF-β1 expression in DN rats and high glucose-induced MCs
As illustrated in Fig. 3A–D, the DN group exhibited remarkably higher level of TGF-β1 mRNA and TGF-β1 when compared to the NC group (P<0.01). Interestingly, SIT-treated group showed significantly lower level of TGF-β1 mRNA and TGF-β1 than DN group (P<0.01). The above indicates that the increased TGF-β1 expression was observed in DN rats through both western blotting and PCR as well as in high glucose-induced MCs by immunofluorescence. SIT-treated groups reduced the increased TGF-β1 expression (Fig. 3E). Taken together, these results suppport that treatment with SIT reverses the overexpression of TGF-β1.
Effects of sitagliptin on p-Smad3 expression in DN rats and high glucose-induced MCs
In Fig. 4A and B, relative quantity of p-Smad3 was upregulated in DN group when compared to the NC group (P<0.01). However, administration of SIT significantly decreased the expression of p-Smad3 compared with DN group (P<0.01). In correlation with the finding from western blotting in DN rats, the p-Smad3 expression in MCs induced by high glucose was increased through immunofluorescence. However, SIT-treated groups could downregulate the increased p-Smad3 expression (Fig. 4C). Thus, the results indicated that administration of SIT significantly decreased the level of p-Smad3 in DN rats and MCs induced by high glucose.
Effects of sitagliptin on Smad7 expression in DN rats and high glucose-induced MCs
The DN group exhibited lower amount of inhibitory Smad7 than NC group (P<0.01). On the contrary, the SIT-treated group showed higher amount of inhibitory Smad7 than DN group (P<0.05) (Fig. 5A–D). In MCs induced by high glucose the levels of Smad7 were remarkably down-regulated compared to those of the NG group at 24 h through ELISA (P<0.01). The levels of Smad7 in SITL, SITM and SITH groups were significantly higher than those of the HG group (P<0.01). No significant difference was found between the MA group and the NG group in terms of the level of Smad7. The Smad7 level in DMSO group was almost identical to that of the HG group, indicating that no obvious effects were generated by the osmotic pressure and the vehicle. The above results indicated that administration of SIT significantly upgraded the levels of Smad7 in DN rats and MCs induced by high glucose.
Discussion
DN is one of the most important causes leading to endstage renal disease, which affects 15–25% of T1DM patients and 30–40% of type 2 diabetes mellitus (T2DM) patients (31,32). DN, also known as nodular diabetic glomerulosclerosis, is characterised by mesangial cell proliferation and excessive accumulation of ECM, which may ultimately lead to chronic renal failure (33). The major ECM proteins such as collagen IV and fibronectin are often used as markers of fibrogenesis in various kidney fibrotic diseases including DN (34–36).
It is now well-known that TGF-β1/Smad3 mediates fibrosis (11,12). In this study, we examined the therapeutic effect of SIT on DN using a rat model of T2DM and high glucose-induced rat MCs. T2DM was induced in rats using a HFD plus repeated low dose STZ injections. Our data showed that the kidney index, the level of 24 h urinary protein, levels of BUN and Cr for the DN rats were significantly increased, indicating a successful renal dysfunction in T2DM rats. The increased intensity of PAS and Masson staining, ECM accumulation also suggested renal lesions in the T2DM rats. These results demonstrated that the T2DM rat model was well established and DN rats were successfully induced renal dysfunction (37). By treating the DN rats with SIT in our study, we found that the SIT significantly inhibited kidney fibrosis and remarkably decreased the levels of proteinuria, BUN and Cr. This indicated that the SIT effectively improved the renal function.
An interesting finding of this study was that SIT works through inhibiting TGF-β1/Smad3 signaling and activating Smad7 expression to alleviate renal fibrosis of the diabetic kidney. It is well-known that both inhibition of TGF-β1/Smad3 pathway and the increasing Smad7 expression results in reducing ECM accumulation (12). Moreover, we also found that the induced DN from T2DM by HFD plus repeated low dose injections of STZ and high glucose-induced rat MCs were all associated with a marked activation of Smad3 but a loss of Smad7, suggesting imbalance between Smad3 and Smad7 signaling in the pathogenesis of DN model. In contrast, treatment with SIT weakened diabetic renal injury by rebalancing the TGF-β1/Smad signaling pathway.
Thus, SIT exhibited potent therapeutic potential in preventing the progression of DN through blocking TGF-β1/Smad3-mediated renal fibrosis and upregulating inhibitory Smad7, which may provide the basis of treatment for DN patients in the future.