Introduction
Obesity is increasing worldwide. More than 1.9 billion adults are overweight and 13% of adults are obese (1). Obesity increases the risk of various chronic diseases, such as cardiovascular diseases, arteriosclerosis and type 2 diabetes, which are severe public health problems (2). Obesity is characterized by an accumulation of lipids via increasing adipogenesis, the inhibition of lipolysis and adenosine monophosphate-activated protein kinase (AMPK) activation (3-5). Adipogenesis is the process of differentiation from preadipocytes to mature adipocytes, which is mediated by several adipogenic transcription factors, such as γ-cytidine-cytidine-adenosine-adenosine-thy midine (CCAAT)/enhancer binding protein (C/EBP)-α, -β and peroxisome proliferator-activated receptor (PPAR)-γ (6,7). The expression of key transcription regulators, including C/EBP-α, C/EBP-β and PPAR-γ, activates various adipogenic genes, including fatty acid synthase (FAS), fatty acid binding protein (FABP4), and glucose transporter 4 (GLUT4) (8,9). Furthermore, lipolysis is a catabolic process that hydrolyzes triglycerides (TG) into glycerol and free fatty acids, and it plays a crucial role in balancing the lipid metabolism of adipose cells (4,10). In particular, hormone-sensitive lipase (HSL) is a major lipolysis gene that controls the hydrolysis of TG by its rate-limiting role (10). Moreover, 5′-AMPK is a regulator of energy homeostasis and plays an important role in regulating adipocyte differentiation (5). Activation of AMPK induces fatty acid oxidation, inhibition of fatty acids synthesis and a reduction in the transcription of adipogenic genes such as C/EBP-α, C/EBP-β, and PPAR-γ (5,11).
Numerous studies have demonstrated that extracts from plants such as Aster glehni, Eclipta alba and yellow capsicum cause a downregulation of adipogenesis and lipogenesis as well as the induction of lipolysis (12-14). Acer okamotoanum is a plant found on Ulleungdo Island (Korea) and contains a number of antioxidant compounds including cleomiscosins A and C (15). Bioactive flavonoids from A. okamotoanum, including quercitrin, isoquercitrin and afzelin have been previously isolated (16). In addition, the sap of A. okamotoanum is reported to have various biological activities such as antioxidant, immune improvement and anti-hypertension effects (17-19). In addition, the authors previously demonstrated that the ethyl acetate (EtOAc) fraction from A. okamotoanum exhibited antioxidant, neuroprotective and cognitive improvement activities (20,21). According to Kim et al (22), the A. okamotoanum Nakai leaf extract suppressed the expression of PPAR-γ and C/EBP-α via the inactivation of phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) signaling and the activation of β-catenin signaling. Therefore, the A. okamotoanum Nakai leaf extract inhibited adipocyte differentiation via adipogenesis in 3T3-L1 cells. Various mechanisms are involved in adipocyte differentiation, such as adipogenesis, lipogenesis, lipolysis and AMPK activation. This study is the first to the best of our knowledge to report on the anti-adipocyte differentiation effects of A. okamotoanum by the regulation of adipogenesis as well as lipolysis and AMPK activation in 3T3-L1 cells.
In the present study, the anti-adipocyte differentiation effect of the EtOAc fraction from A. okamotoanum on the differentiation of 3T3-L1 cells was investigated. Furthermore, the molecular mechanisms in the protective role of adipocyte differentiation of the A. okamotoanum EtOAc fraction were associated with the regulatory pathways of adipogenesis, lipolysis and the activation of AMPK.
Materials and methods
Reagents
Dulbecco's modified eagle medium (DMEM), bovine calf serum (BCS), fetal bovine serum (FBS), penicillin-streptomycin and trypsin-EDTA solution were purchased from Welgene, Inc. 3-Isobutyl-1-methylxanthine (IBMX), dexamethasone and insulin were purchased from Sigma-Aldrich; Merck KGaA. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was obtained from Bio Basic, Inc., and dimethyl sulfoxide (DMSO) was purchased from Bio Pure.eu GmbH. All primary and secondary antibodies were purchased from Cell Signaling Technology, Inc.
Sample preparation
A. okamotoanum was collected from Ulleung-do, Korea. A voucher specimen was deposited at the Department of Plant Science and Technology, Chung-ang University, Anseong, Korea (Voucher no. LEE 2014-04). The dried aerial portion of A. okamotoanum (995.4 g) was extracted eight times in methanol (MeOH) using a rotary evaporator. The MeOH extract was suspended in distilled water and then fractionated and dried successively with n-hexane, CH2Cl2, EtOAc, and n-BuOH. The EtOAc fraction (35.0 g) was used in the present study (16).
Cell culture and differentiation
The 3T3-L1 pre-adipocyte cells were obtained from the American Type Culture Collection. The cells were cultured in DMEM supplemented with 10% BCS and 1% penicillin-streptomycin at 37°C in humidified air with 5% CO2 in an incubator. To induce differentiation, 100% confluent 3T3-L1 pre-adipocytes were stimulated with 0.5 mM IBMX, 1 µM dexamethasone and 5 µg/ml insulin in DMEM containing 10% FBS [methylisobu-tylxanthine, dexamethasone, insulin (MDI) media] for 2 days. The MDI media was replaced with differentiation media (5 µg/ml insulin in DMEM containing 10% FBS). The cell culture media was changed 4 times every 2 days.
Cell viability
The 3T3-L1 cells were seeded at a density of 1×105 cells/ml in a 24 well plate and then incubated for 24 h. Afterward, the EtOAc fraction of A. okamotoanum was added to the test wells at various concentrations (1-500 µg/ml) and then incubated at 37°C for 72 h. The cell viability was determined using an MTT assay (23). The MTT solution was replaced with DMEM media (5 mg/ml) in the wells followed by incubation at 37°C for 4 h. The formazan crystals were dissolved in DMSO and the absorbance was read at 540 nm using a microplate reader (Thermo Fisher Scientific, Inc.).
Oil Red O staining
The cells were washed with PBS, fixed with 10% formalin at 25°C for 10 min and washed with PBS and 60% isopropanol. Cells were then stained with 0.6% Oil Red O solution at 25°C for 20 min, washed 4 times with PBS and 60% isopropanol, and images were captured. For quantitative analysis, Oil Red O stain was eluted with 100% isopropanol and quantified by measuring the absor-bance at 500 nm (24).
Western blot analysis
The cells were harvested using a cell scraper and lysed with radioimmunoprecipitation assay buffer (Elpis Biotech, Inc.) containing protease inhibitor cocktail at 4°C for 1 h. The protein concentration was determined using a Bio-Rad protein assay (Bio-Rad Laboratories, Inc.). Equal amounts of protein (15 µg) were separated with 8-13% SDS-PAGE and transferred onto a polyvinylidene fluoride membrane. The membrane was blocked with 5% skim milk at room temperature for 1 h followed by incubation with the following primary antibodies: β-actin (cat. no. 8457; 1:1,000), C/EBP-α (cat. no. 2295; 1:200), C/EBPβ (cat. no. 3087; 1:200), PPAR-γ (cat. no. 2430; 1:200), FAS (cat. no. 3189; 1:200), FABP4 (cat. no. 2120; 1:200), GLUT4 (cat. no. 2213; 1:200), phospho-HSL (cat. no. 4126; 1:200), HSL (cat. no. 4107; 1:200), phospho-AMPK (cat. no. 2535; 1:200), or AMPK (cat. no. 2532; 1:200) overnight at 4°C. Next, the membranes were incubated with the secondary antibodies anti-rabbit IgG conjugated to horseradish peroxidase (HRP) (cat. no. 7074; 1:500) and anti-mouse IgG conjugated to HRP (cat. no. 7076; 1:500) at room temperature for 1 h, activated with ECL substrate solution (Clarity Western ECL Substrate kit; Bio-Rad Laboratories, Inc.) and visualized with the Davinchchemi™ Chemiluminescence Imaging system (Davinch Mini Chemi Q6; Davinch-K Co. Ltd.). Quantification of western blot band intensity was performed using ImageJ software (version 1.51p; National Institutes of Health).
Statistical analyses
Each experiment was performed in triplicate (n=3). All data are expressed as the mean ± standard deviation. The results were assessed by one-way analysis of variance followed by Duncan's multiple range test using IBM SPSS statistics software (version 20.0, IBM Corporation). P<0.05 was considered to indicate a statistically significant difference.
Results
Effect of A. okamotoanum on 3T3-L1 cell viability
To evaluate the cytotoxicity of the EtOAc fraction of A. okamotoanum on 3T3-L1 cells, cell viability was investigated using an MTT assay. The 3T3-L1 adipocytes were treated with various concentrations (1-500 µg/ml) of the EtOAc fraction of A. okamotoanum for 72 h. As shown in Fig. 1, the EtOAc fraction of A. okamotoanum at concentrations up to 10 µg/ml did not exhibit significant cytotoxicity. However, cell viability was significantly reduced with 25-500 µg/ml of the EtOAc fraction of A. okamotoanum compared with the untreated controls (P<0.05). Therefore, the EtOAc fractions of A. okamotoanum at concentrations of 1, 2.5, 5 and 10 µg/ml were used for further experiments.
Effect of A. okamotoanum on lipid accumulation in 3T3-L1 cells
To confirm whether the EtOAc fraction of A. okamotoanum inhibited adipocyte differentiation, differentiated 3T3-L1 cells were treated with various concentrations (1, 2.5, 5 or 10 µg/ml) of the EtOAc fraction of A. okamotoanum. As shown in Fig. 2, the number of lipid droplets increased in MDI-treated differentiated 3T3-L1 cells when compared with the undifferentiated cells. However, treatment with the EtOAc fraction of A. okamotoanum decreased lipid accumulation; this was observed using Oil Red O staining. In addition, the MDI-treated control had significantly increased intracellular TG levels of 7.76-100.00% compared with the normal group (P<0.05; Fig. 3). In contrast, the treatment with the EtOAc fraction of A. okamotoanum significantly decreased intracellular TG levels (P<0.05). The treatment with the EtOAc fraction of A. okamotoanum at doses of 1, 2.5 and 5 µg/ml slightly decreased TG levels to 97.16, 91.75, and 87.29%, respectively. However, treatment with 10 µg/ml of the EtOAc fraction of A. okamotoanum markedly inhibited TG levels by 17.60% as compared with the controls.
Effects of A. okamotoanum on the expression of adipogenic transcription factors in 3T3-L1 cells
To investigate the anti-adipogenesis effects of the EtOAc fraction of A. okamotoanum, the protein expression of adipogenic transcription factors, including C/EBP-α, C/EB-Pβ and PPAR-γ was measured. As shown in Fig. 4, the protein levels of C/EBP-α, C/EBP-β and PPAR-γ were significantly upregulated in differentiated 3T3-L1 cells compared with undifferentiated cells (P<0.05). However, the treatment with the EtOAc fraction of A. okamotoanum inhibited the protein expression of C/EBP-α, C/EBP-β and PPAR-γ (P<0.05). Of the adipogenic transcription factors, the EtOAc fraction of A. okamotoanum most effectively suppressed C/EBPβ in a dose-dependent manner.
Effects of A. okamotoanum on adipogenesis-related factors in 3T3-L1 cells
To investigate the effect of the EtOAc fraction of A. okamotoanum on adipogenesis-related factors, the protein expression of FAS, FABP4 and GLUT4 was confirmed (Fig. 5). The protein expression of FAS, FABP4 and GLUT4 were increased in the differentiated 3T3-L1 cells compared with the undifferentiated control cells (P<0.05). However, the present results revealed that the treatment with the EtOAc fraction of A. okamotoanum at concentrations of 5 and 10 µg/ml significantly downregulated FAS and FABP4 (P<0.05). GLUT4 protein expression was also significantly decreased by the EtOAc fraction of A. okamotoanum compared with the control (P<0.05).
Effects of A. okamotoanum on lipolysis related factors in 3T3-L1 cells
The effect of the EtOAc fraction of A. okamotoanum on lipolysis was determined by measuring the protein expression of HSL and phosphorylated HSL (Fig. 6A). The phosphorylation of HSL was significantly reduced in the differentiated 3T3-L1 cells compared with the undifferentiated cells (P<0.05). Treatment with the EtOAc fraction of A. okamotoanum significantly upregulated the phosphorylation of HSL (P<0.05).
Effects of A. okamotoanum on activation of AMPK in 3T3-L1 cells
To examine whether the EtOAc fraction of A. okamotoanum affected the activity of AMPK, the levels of AMPK and phosphorylated AMPK were measured (Fig. 6B). The MDI-treated differentiated 3T3-L1 cells suppressed phosphorylation of AMPK compared with undifferentiated 3T3-L1 cells (P<0.05). However, the EtOAc fraction of A. okamotoanum significantly increased the phosphorylation of AMPK compared with the control (P<0.05).
Discussion
In obesity, the accumulation of lipids and the differentiation of adipocytes in adipose tissue can lead to abnormal lipid metabolism, which can increase the risk of chronic diseases (2). Adipose tissue is dependent on the differentiation of preadipocytes to adipocytes; therefore, 3T3-L1 preadipocytes have been widely used to study the differentiation of adipocytes in vitro (25). During adipocyte differentiation, key adipogenic transcription factors, including C/EBP-β, C/EBP-α and PPAR-γ, are activated resulting in intracellular fat accumulation (26). Activation of adipogenic transcription factors induces target genes that determine the phenotypes of mature adipocytes (26,27). These target genes are primarily associated with lipogenesis, TG hydrolysis and glucose and fatty acid metabolism (26,27). Differentiated 3T3-L1 cells treated with MDI mimics the development of obesity in humans and also possesses adipocyte structures similar to live adipose tissue (25,28). In addition, several natural extracts and bioactive compounds have been commonly used to produce anti-obesity effects in a differentiated 3T3-L1 cell model (12,13). The EtOAc fraction of A. okamotoanum showed the highest protective effect from free radicals and oxidative stress among other fractions and extracts (20). In addition, the EtOAc fraction from A. okamotoanum exhibited anti-oxidative stress, neuroprotective effects and cognitive improvement activity (20,21). Oxidative stress is closely associated with obesity and antioxidant supplements are beneficial in the management of obesity (29). In addition, the authors previously reported determination of active compounds such as quercitrin, isoquercitrin and afzelin from EtOAc fraction of A. okamotoanum, which had antioxidant activity (16). Therefore, the anti-differentiation effect of the EtOAc fraction of A. okamotoanum and its molecular mechanisms were studied by measuring the expression of adipogenesis and lipolysis-related factors in 3T3-L1 cells.
The present results showed that lipid droplets were markedly increased in differentiated 3T3-L1 cells after stimulation with MDI. However, the EtOAc fraction of A. okamotoanum decreased the lipid droplets in differentiated 3T3-L1 cells. Cytotoxicity of the EtOAc fraction of A. okamotoanum was observed. Treatment with the EtOAc fraction of A. okamo- toanum at concentrations up to 10 µg/ml did not show significant cytotoxicity compared with untreated control cells; therefore, concentrations of 1, 2.5, 5 and 10 µg/ml of the EtOAc fraction of A. okamotoanum were used. TG levels were measured quantitatively. Treatment with the EtOAc fraction of A. okamotoanum at concentrations of 1, 2.5, 5 and 10 µg/ml decreased TGs compared with the control. The inhibitory effect of TG accumulation was dramatically elevated at the concentration of 10 µg/ml and it was relatively weak at the concentrations of 1-5 µg/ml. Therefore, it is suggested that A. okamotoanum effectively inhibits lipid formation.
Adipogenesis is the differentiation process of adipocytes from preadipocytes and it is characterized by intracellular lipid accumulation. During adipocyte differentiation, key adipogenic transcription factors, such as C/EBP-α, C/EBP-β and PPAR-γ, are expressed. The expression of C/EBP-β activates C/EBP-α, PPAR-γ and other adipogenic genes. The over-expression of C/EBP-α and PPAR-γ is known to directly affect the development of fat cells (8). In the present study, differentiated 3T3-L1 cells treated with MDI showed an upregulation of key transcription genes related to adipo-genesis, including C/EBP-α, C/EBP-β and PPAR-γ. However, differentiated 3T3-L1 cells treated with the EtOAc fraction of A. okamotoanum showed downregulation of key transcription genes. The regulatory effect on adipogenesis factors of the EtOAc fraction of A. okamotoanum was not dose-dependent except with C/EBP-β. The activation of C/EBP-α and PPAR-γ are associated with lipid metabolism factors such as FAS, FABP4, and GLUT4. FAS is highly expressed in adipose tissue; therefore, it plays an important role in lipogenesis (30). FAS catalyzes the synthesis of fatty acids and the cytoplasmic storage of TG (30). FABP4, a terminal adipocyte differentiation marker gene, is directly associated with lipogenesis; it induces the accumulation of lipid droplets in the cytoplasm of differentiated 3T3-L1 cells (31). GLUT4 plays a role in lipogenesis through the insulin signaling pathway in differentiated 3T3-L1 adipocytes (32). GLUT4 is involved in the insulin-stimulated glucose uptake by adipose tissue and skeletal muscle, increasing the risk of developing obesity and diabetes (33). The results of the present study indicate that the EtOAc fraction of A. okamotoanum inhibited adipogenesis in adipocytes through the downregulation of FABP4, FAS and GLUT4 in differentiated 3T3-L1 cells.
Lipolysis is the catabolic process of releasing fat from adipose tissue. Lipolysis is the chemical decomposition of TG into glycerol and fatty acids, which blocks lipid accumulation (34). In addition, lipolysis controls lipid homeostasis in adipocytes (34). Therefore, the reduction of lipid accumulation and the increase of lipid catabolism in adipocytes are crucial to the development of anti-obesity agents. During the lipolysis of adipocytes, HSL is an important lipolytic factor. HSL hydrolyzes diglycerides and free fatty acids in TG hydrolysis. HSL is activated via phosphorylation by cAMP-dependent protein kinase A at Ser 660, which stimulates HSL to hydrolyze TGs (35). HSL is a rate-limiting enzyme for lipid mobilization reactions and hydrolyzes diglycerides in the mobilization of TG stored in adipocytes (36). In addition, elevated HSL expression can catalyze adipose lipolysis in response to β-adrenergic stimulation (37). In this study, treatment with the EtOAc fraction of A. okamotoanum increased lipolysis by upregulation of HSL activation in differentiated 3T3-L1 cells. In particular, the lipolysis factor ratio of phosphorylated-HSL/HSL was upregu-lated dose-dependently compared with the control. Therefore, it is suggested that the EtOAc fraction of A. okamotoanum could play a potential role in the lipid catabolic process.
The present study also investigated AMPK protein expression in the presence/absence of the EtOAc fraction of A. okamotoanum in differentiated 3T3-L1 cells. AMPK is a metabolic gene that is involved in the regulation of lipid metabolism (38). The activation of AMPK in adipose tissue suppresses lipid synthesis and lipogenesis, regulates fatty acid synthesis, and enhances fatty acid oxidation and glucose transport (38,39). Furthermore, AMPK activity enhances lipolysis by phosphorylation of HSL (39,40). To develop anti-obesity agents, numerous researchers have investigated the AMPK activity of natural extracts and their bioactive compounds in adipocytes (41,42). Therefore, AMPK activity is important to the development of an obesity treatment strategy. In the present study, it was demonstrated that the EtOAc fraction of A. okamotoanum induced the activation of AMPK signaling in differentiated 3T3-L1 cells. This suggests that the EtOAc fraction of A. okamotoanum inhibited adipogenesis and upregulated lipolysis by the regulation of the AMPK signaling pathway. In the present study, the EtOAc fraction of A. okamotoanum inhibited TG accumulation during the differentiation of the 3T3-L1 cells. In addition, A. okamotoanum suppressed adipogenic transcription factors and adipogenesis-related protein expression. Furthermore, A. okamotoanum enhanced the expression of lipolytic proteins, such as HSL and also activated AMPK signaling. Conjugated linoleic acid (CLA) is widely used to treat obesity and it has been reported that CLA is very effective at decreasing body fat accumulation (43). Previous studies demonstrated that CLA inhibited adipocyte differentiation in 3T3-L1 cells by regulation of adipogenesis, lipolysis and AMPK signaling (43-45). Treatment with 10 µg/ml EtOAc fraction of A. okamotoanum inhibited intracellular TGs more effectively than treatment with 3T3-L1 cells with 100 µM CLA (43). In addition, A. okamotoanum at concentrations of 10 µg/ml decreased the protein expression of PPAR-γ, which was similar to that in 100 µM CLA-treated 3T3-L1 cells (43). Based on these findings, the present study suggested that A. okamotoanum has regulatory activity on adipocyte differentiation and its effect is similar to that of CLA.
Acer (maple) is commonly used in commercial products such as maple syrup and seed oil (46). In addition, several Acer species have been used in traditional medicine for detoxification and to treat rheumatism, eye disease, hepatic diseases, and hemostasis (46). Recently, numerous studies have reported the pharmacological activities of Acer species, including antioxidant, anti-tumor, anti-inflammation, antibacterial, antihyperglycemic, hepatoprotective and anti-obesity activities (46). The EtOAc fraction of A. truncatum Bunge significantly reduced body weight by the inhibition of lipogenesis-related factors such as FAS in vivo (47,48). In addition, Kim et al (22) demonstrated that A. okamotoanum regulated adipocyte differentiation by several molecular mechanisms related to adipogenesis, including PI3K/Akt and β-catenin/glycogen synthase kinase (GSK)3β. A previous study demonstrated that the MeOH extract of leaves from A. okamotoanum inhibited the phosphorylation of mammalian target of rapamycin and P70S6K by attenuating the PI3K/Akt pathway, thereby suppressing key adipogenic transcription genes (22). In addition, A. okamotoanum induced the activation of β-catenin/GSK3β, promoting the downregulation of PPAR-γ (22). Therefore, A. okamotoanum inhibits adipogenesis via regulation of PI3K/Akt and β-catenin/GSK3β signaling. The authors previously isolated and identified flavonoid glyco-sylates such as isoquercitrin (quercetin-3-β-D-glucoside; IQ), quercitrin (quercetin-3-β-D-rhamnoside; QU), and afzelin (keampferol-3-rhamnoside; AF) from the EtOAc fraction of A. okamotoanum (16). IQ inhibited adipocyte differentiation and lipogenesis by the downregulation of adipogenic transcription factors in 3T3-L1 adipocyte cells (49). In addition, IQ exhibited anti-obesity effects by reducing body weight and regulating lipid metabolism in high fat diet-fed mice (49). Furthermore, aglycones of IQ, QU and AF, such as quercetin and kaempferol, showed anti-obesity activity through the regulation of adipogenesis, inflammation, and oxidative stress (50-52). Therefore, flavonoid glycosylates are primarily responsible for the anti-obesity effects of the EtOAc fraction of A. okamotoanum.
In conclusion, the present study suggests that A. okamotoanum inhibits adipocyte differentiation via adipo-genesis and lipolysis in cells. A. okamotoanum could be a promising therapeutic agent against obesity, although further in vivo studies must be done.