Microwaves can be arbitrary and basically defined as extremely high frequency radiowaves created by several types of transmitters, including chip and antenna for mobile phone or ‘magnetron’ for kitchen microwaves (1). With wavelengths of merely a couple of centimeters, microwave ovens cause fat molecules and water vibration, making these substances hot and allowing the capacity to cook many types of food (2). Microwaves used by fixed traffic speed cameras or radar for aircraft ships and weather for casters commonly emit bursts of microwaves, detecting echoes coming back from the objects they strike, and using the time it takes for the echoes to return to determine the distance to a particular object or location (1,2). Contact with certain types of microwaves, together with cordless phones, Wi-Fi, walkie-talkies, ham radio transmitters and other devices are not considered harmful exposure, whereas these types of microwaves constitute the same risk as using a cell phone. Indeed, the body tissue, mostly in the lower body has good conductivity and absorbance of radiation. For example, men wearing cell phones near their groin could reduce their sperm count by 30% (3). Accordingly, cell phone radiation decreases as the distance from the cell phone increases, resulting in marked effects, even if the cell phone is only a short distance away. By contrast, the use of headsets connected to the cell phone may greatly reduce the absorbed cell phone radiation (4), while wearing an air-tube headset has been found to intensify radiation into the ear canal (5), the wire being a transmitter of radiation and serving as an antenna that can attract electromagnetic fields (EMFs) from the surroundings (6). Microwaves used by mobile cells are generated by a small antenna and are transmitted by a line of sight to the transmitter. The prolonged exposure to microwaves has been associated with negative effects on the eyes (7) and recent findings indicated that holding the transmitter of a cell phone close to the head induces detrimental effects on the brain (8,9).

Investigations have suggested that using a cell phone for 10 years or more may significantly increase the risk of developing a tumor (10) thereby doubling the risk of developing glioma or acoustic neuroma in the more exposed brain hemisphere (11). Long-term cell phone use is associated with the likelihood of being hospitalized for migraines and vertigo by 10–20% (8) since the mobile phone signal is absorbed deeply into the brain, up to 2 inches into the adult skull (12). In children, their developing skulls offers a higher opportunity for radiation to penetrate more deeply (13), and thus it is crucial to emphasize the vulnerability of youth brains to microwaves (14). It has been previously shown that adult brain almost entirely absorbs radiations on the ipsilateral part where the phone is held, but not on the contralateral part. Consequently, the ipsilateral temporal lobe or cerebellum absorbed 50–60 or 12–25%, respectively, of the total radiation, while the insular region and overlying skull, scalp and parotid gland tissues are also exposed (15). Additionally, the exposition seems to be even higher in children owing to thinner scalps and skulls, increased water content of their brain and lower brain volume (16). The results of studies on radiofrequency EMFs examining the effects of mobile phone-related exposures at the level of the head on physiological functions such as brain electrical activity (EEG), cognition, brain vascularization, cardiovascular and endocrine systems were not conclusive. The main observation was the effects on the amplitude of EEG α wave during sleep while there was no consensus on the cognitive effects. However, the definite association of EEG α wave with cerebrovascular effects requires further investigation (17). In rat, exposition to low-power-density microwave fields resulted in increased levels of plasma corticosterone and glucocorticoid receptor nuclear translocation, as well as apoptosis in the hippocampus affecting spatial learning and memory (18), following exposure to a dose of microwaves comparable to those received by human in normal utilization (10).

In agreement with these data, investigations have been conducted into mobile phone handsets and findings indicate that some phones can cause higher rates of brain tumors (19) including new mobile phones, which by contrast to outdated analogue phones, emit much stronger signals that may be damaging to the health. However, despite these data, the overall scientific opinion states that the benefits of mobile phones seem to outweigh any danger, albeit the utilization of cell phones must respect a precautionary approach, according to conclusions of the Stewart Report published in 2000 (20). Nevertheless, data arguing in favor of the possibility that phone microwave range may be harmful to the human health (21), whether the long-term effects of a cell phone can induce the development of Alzheimer's disease (AD), the state of dementia and neurodegenerative disease that occurs when the nerve cells in the brain die remains to be determined (22,23). In this review, we examine whether microwaves cause AD.

The introduction of cell phones, Wi-Fi, satellite and wireless communications and the microwave oven have had a significant impact on daily life. However, there are growing concerns regarding the potential health risks associated with increased, frequency, modulation frequency and duration of microwave exposure (24,25) and particularly, the risk of developing AD, an incurable and debilitating disease affecting individuals worldwide being associated with the death of nerve cells in the brain, cerebrovascular impairments and dementia (22,23).

An increase of major symptoms has become evident for individuals residing at a distance of <500 m from a mobile phone base station, including irritability, headache, and lower cognitive performance (26). Of note, an increase of intracellular Ca²⁺ levels that coincided with the overactivation of the GABA receptor NMDA, which activates CaMKII, has been observed in cell cultures exposed to microwaves. NMDA is important in the regulation of induction of synaptic plasticity modifications such as long-term potentiation (LTP) (27), a physiological mechanism of learning memory formation that was actually inhibited by microwave (25), suggesting that exposure to microwaves can result in alterations of synaptic structure, amino acid neurotransmitter release, calcium influx and NMDA receptor signaling molecules (microwave exposure impairs synaptic plasticity in the rat hippocampus and PC12 cells through overactivation of the NMDA receptor signaling pathway) (28). Accordingly, experimental studies have revealed that long-term microwave exposure, even low levels of radiation power (29,30), were associated with a significant decrease in learning and memory activity reminiscent of neuronal degeneration and enlargement perivascular spaces in the hippocampus that produce adverse biological effects in the nervous system, while the effects on cognitive functions remained controversial (31–33). Notably, the low-level and long-term microwave exposure of rats have led to marked alteration in the structure and function of the hippocampus, including mitochondrial swelling, cristae disorders (34,35) as well as an increase in the concentration of neurotransmitters glutamate (Glu), aspartic acid (Asp), glycine (Gly) and γ aminobutyric acid (GABA) (34). The rough endoplasmic reticulum also exhibited sacculated distension and there was a decrease in the quantity of synaptic vesicles, suggesting detrimental effects of microwaves on synaptic plasticity and cognitive function, especially in learning and memory (36,37). In line with these data, Glu, Gly, Asp and GABA are neurotransmitters playing critical roles in processes such as learning and memory activities (38,39), sleep-wake cycle (40) response (41), induction of pain (42) reaction time in behavioral and brain plasticity (43) as well as in various central nervous system (CNS) diseases (44). Taken together, these data indicate serious hazards related to the overuse of the cell phone, and thus mostly provide detrimental potential effects of microwaves on the brain that may lead to the development of AD. Nevertheless, some reports in animal models suggest stimulatory or beneficial effects following exposure to cell phone radiofrequency radiation (45). For example, beneficial cognitive effects, including a decrease in reaction time after human short-term exposure to cell phone radiation or occupational exposure to radar microwave after short-term exposure to radar microwave radiation have been documented in rats (46,47). Such studies found that exposure may exhibit an increase in brain glucose consumption and suggested that radiofrequency radiation may protect against cognitive impairment in AD (45,46,48,49).

The impact of wireless communication on human health is a mater of debate. Since there are widespread concerns regarding the deleterious effects of the exposure to microwaves on human tissues and the subsequent potential threat of carcinogenesis, we can conclude that the current exposure to microwaves during the use of cell phones is not safe for long-term exposure, despite the current scientific opinion. Absorption of the cell phone signal into the brain of children does not exclude serious neuronal damage, as evidenced in rat studies (50). In addition, the increased risk of tumors of the head associated with long-term cell phone use is evident since radiofrequency may cause the blood-brain barrier to leak and to favor the damage of genetic material which consists of common precursors to cancer (51). Accordingly, poor fertility and the increased chance of miscarriage and childhood cancer have been associated with cell phone storage in front pockets. Notably, the data suggested that the hippocampus can be injured by long-term microwave exposure (52), which may result in the impairment of cognitive function due to neurotransmitter disruption. These results suggest that precautionary approach underlying the restrictive use of cell phones constitutes essential appropriate guidelines to follow although additional studies are needed.