MCO Molecular and Clinical Oncology 2049-9450 2049-9469 D.A. Spandidos 10.3892/mco.2017.1254 MCO-0-0-1254 Articles Erythropoietin for cancer-associated malignant anemia: A meta-analysis ZhaoFeng 1 * WangYijuan 1 * LiuLin 2 BianMeiling 3 Department of Blood Transfusion Medicine, Linzi District People's Hospital, Binzhou Medical University, Zibo, Shandong 255400, P.R. China Medical Intensive Care Unit, PKU Care Luzhong Hospital, Zibo, Shandong 255400, P.R. China Department of Internal Medicine, Linzi District People's Hospital, Binzhou Medical University, Zibo, Shandong 255400, P.R. China Correspondence to: Dr Meiling Bian, Department of Internal Medicine, Linzi District People's Hospital, Binzhou Medical University, 139 Huangong Road, Linzi, Zibo, Shandong 255400, P.R. China, E-mail: meiling_bian@126.com

Contributed equally

 06 2017 08 05 2017 6 6 925 930 17102016 23032017 Copyright © 2017, Spandidos Publications 2017

The present study aimed to evaluate the effectiveness of erythropoietin (EPO) for improving cancer-associated malignant anemia. A search was performed for randomized clinical trials, conducted according to the Cochrane manual, using electronic databases including PubMed, EMBASE, the Cochrane Library and ClinicalTrails.gov up to 15 August 2015. A total of 6 eligible studies from 5 articles enrolling a total of 453 patients were entered into the current meta-analysis. Upon EPO treatment, there were significant differences in the change in hemoglobin (HB) levels compared with the placebo at short-term follow-up [mean difference (MD)=0.66; 95% confidence interval (CI), 0.14–1.18; I2=Not applicable; P=0.01) and long-term follow-up (MD=0.10; 95% CI, 0.02–0.18; I2=Not applicable; P=0.01) under the random effects model. For changes in hematocrit (HCT) compared with the placebo, the results revealed there were significant differences at short-term follow-up (MD=2.47; 95% CI, 0.75–4.19; I2=Not applicable; P=0.005) and long-term follow-up (MD=7.60; 95% CI, 6.15–9.05; I2=Not applicable; P<0.00001) under the random effects model. Compared with the placebo in short-term follow-up under the fixed effects model with homogeneity, the result was a significant difference for the transfusion ratio [relative risk (RR)=0.81; 95% CI, 0.67- 0.97; I2=34%; P=0.02) and the transfusion requirements (MD=−0.45; 95% CI, −0.92, 0.03; I2=6%; P=0.07). Funnel plots did not detect any publication bias. These results suggest that EPO was beneficial to alleviate cancer-associated anemia and improve survival outcomes for patients with cancer.

 erythropoietin cancer-associated malignant anemia hemoglobin meta-analysis Introduction

Cancer anemia, also known as cancer-associated malignant anemia, is a common clinical symptom of cancer, with a rate of incidence approaching 50% of cancer cases (1,2). There are several causes of cancer-associated malignant anemia, such as bleeding, hemolysis, lack of nutrition, bone marrow necrosis and fibrosis caused by tumor cell infiltration, direct inhibition of red blood cells caused by radiotherapy and chemotherapy, relative insufficiency of erythropoietin (EPO) secretion (3), hematopoietic inhibition and influence of iron metabolism and reduced reactivity of EPO and cytokines from cancerous human bone marrow (4–6). Malignant tumor anemia is a common complication in various malignant tumors, in which red blood cell depletion leads to inadequate tissue oxygenation, reducing the sensitivity of tumors to radiation and chemotherapy, thereby lowering the patient's quality of life, decreasing survival time and affecting overall prognosis (4,7,8). At 3 years post-cancer diagnosis, mortality among patients with anemia is twice as high as that among those without anemia (9–12). Clinically speaking, quality of life and adverse events are important predictors in patients with cancer (8).

At present, there are four methods for treating cancer-associated anemia: Blood transfusion (primarily red blood cell suspension), iron supplements (reduced iron deposits caused by EPO treatment), change of treatment scheme and administration of stimulating factor (4,13). Blood transfusion is not without risks, particularly for patients with cancer. Blood transfusions may decrease the survival rate, and tumor recurrence may occur following blood transfusion, which may lead to poor prognosis. The effect of iron supplements alone is often slow, with low efficacy (2,5). EPO is a type of high-purity active glycoprotein produced using genetically modified technology, and it specifically stimulates bone marrow hematopoietic cells (13–15). Although a large body of research has demonstrated that the EPO is able to improve the symptoms of cancer-associated malignant anemia, this conclusion remains controversial. In order to assess the efficacy of EPO in this setting, the current study conducted a meta-analysis of the available research.

 Materials and methods <sec> <title>Search strategy

A series of electronic searches of PubMed, EMBASE, the Cochrane Library and ClinicalTrails.gov up to8 February 2016 for eligible randomized or parallel-group design clinical trials investigating EPO for the treatment of cancer-associated malignant anemia was performed using the following Medical Subject Headings and text words: ‘neoplasm*’, ‘tumor*’, ‘cancer*’, ‘erythropoietin’ and ‘anaemia*’.

 Inclusion criteria

All studies were selected in accordance with following inclusion criteria: (1) All studies were randomized or parallel-group design clinical trials; (2) eligible studies included patients with cancer-associated malignant anemia patients older than 18 years; (3) the entire study population was patients diagnosed with cancer with malignant solid tumors confirmed by histology or cytology; (4) the hemoglobin (HB) levels of the patients were >8.5 and <13.5 g/dl; (5) eligible studies included at least one of the following outcomes: Change in HB, change in hematocrit (HCT), the ratio of transfusion and transfusion requirements during the treatment.

 Exclusion criteria

Studies were excluded in accordance with following criteria: (1) The study reported that the patients had received radiation, chemotherapy, or surgery prior to or during receiving EPO; (2) anemia was not caused by cancer; (3) the study included primary hematologic disease, seizure disorder, uncontrolled hypertension, recent history of thromboembolic disease (within 1 year), other clinically significant systemic disease, an active infectious process, pregnancy, ongoing blood loss, scheduled autologous blood donation or blood transfusion within the previous 30 days; (4) duplicate published research was excluded; (5) the data could not be extracted or obtained through contact with the original author.

 Data extraction

The relevant information including study design, patients' characteristics, the criteria of tumor staging, tumor staging, the initial HB levels, interventions, controls and outcomes (change in HB, change in HCT, the ratio of transfusion or transfusion requirements) were independently extracted and entered it into a database by two investigators. When relevant research information was missing, particularly design or outcomes, the original authors were contacted for clarifications. Then, intention-to-treat (ITT) datasets were used for all outcomes whenever available. Disagreements between the two authors on data extraction were resolved by discussion. If the dispute persisted, other senior investigators were consulted to reach a consensus.

 Statistical analysis

For the outcomes based on dichotomous data (16,17), relative risk (RR) as was utilized as an effect estimator, while for continuous outcomes mean difference (MD) was used (17,18). Subsequently, 95% confidence intervals (CIs) and P-values were calculated using RevMan 5.3 software (Cochrane Collaboration, London, UK) in all outcomes. A statistical test for heterogeneity was performed and an I2>40 was adopted as evidence for heterogeneity according to the Cochrane handbook (19). If the data were heterogeneous under the fixed effects model, a random effects model was utilized (20). Through observing asymmetry of funnel plots, publication bias was evaluated qualitatively (21). In a funnel plot, larger studies that provide a more precise estimate of an intervention's effect form the spout of the funnel, whereas smaller studies with less precision form the cone end of the funnel. Asymmetry in the funnel plot indicates potential publication bias. P≤0.05 was considered to indicate a statistically significant difference.

 Results <sec> <title>Study selection and data collection

In total, 453 publications were obtained from electronic databases (Fig. 1). Based on the selection criteria, quantitative data was obtained for the current meta-analysis by reading all titles, abstracts and performing full text evaluations. Finally, 6 eligible studies from 5 articles were entered into the meta-analysis.

 Study characteristics

The search obtained 6 eligible studies from 5 articles (22–26), which enrolled a total of 595 patients. The change in HB was reported in 2 studies (23,26), the transfusion requirements were noted from 3 studies (23,25), the ratio of transfusion was obtained from a total of 233 of 495 patients from 5 studies (22–26) and the change in HCT was reported in 2 studies (23,26). Table I presents the clinical characteristics from all studies.

 Change in HB

The change in HB levels is presented in Fig. 2; each included study suggested statistically significant improvements upon treatment with EPO. Compared with placebo, the results revealed there were significant differences in the short-term follow-up [MD=0.66; 95% CI, 0.14–1.18; I2=Not applicable (NA); P=0.01] and the long-term follow-up (MD=0.10; 95% CI, 0.02–0.18; I2=NA; P=0.01). However, the overall result revealed that there was not a significant difference (MD=0.32; 95% CI, −0.02–0.85; I2=77%; P=0.24) under substantial heterogeneity.

 Change in HCT

As presented in Fig. 3, each included research suggested that there were statistically significant changes in HCT upon treatment with EPO. Compared with placebo, the results showed there were significant differences at both the short-term follow-up (MD=2.47, 95% CI, 0.75–4.19; I2=NA;P=0.005) and the long-term follow-up (MD=7.60; 95% CI, 6.15–9.05; I2=NA; P<0.00001). The overall result also revealed that there was a significant difference (MD=5.06; 95% CI, 0.03–10.08; I2=95%; P=0.05) under substantial homogeneity.

 Ratio of transfusion

As presented in Fig. 4, for the ratio of transfusion only one study (24) suggested statistically significant improvements, upon EPO treatment. Compared with placebo, the overall meta-analysis result revealed that there was a significant difference (RR=0.81; 95% CI, 0.67–0.97; I2=34%; P=0.02) at short-term follow-up under moderate homogeneity.

 Transfusion requirements

For the transfusion requirements, as presented in Fig. 5, each included research suggested that there were no statistically significant differences, although the MD value was advantageous to EPO. Compared with placebo, the overall result revealed that there was no significant difference (MD=−0.45; 95% CI, −0.92–0.03; I2=6%; P=0.07) at short-term follow-up under weak homogeneity.

 Publication bias

There was no potential publication bias based on symmetry from a funnel plot of the ratio of transfusion outcome (Fig. 6).

 Discussion

There are several potential causes of cancer-associated malignant anemia. These include bleeding, hemolysis, poor nutrition, radiotherapy and chemotherapy. However, as anemia reflects a shortage of red blood cells, which are produced in the bone marrow, cancer-associated anemia can also be caused by the inability of the bone marrow to produce them due to a relative deficiency of EPO. To establish if EPO significantly increased HB levels, and whether this was associated with improved energy levels and a reduction in blood transfusions, the current meta-analysis was performed with 6 eligible studies from 5 articles that included patients with cancer-associated anemia. The efficacy of EPO for cancer-associated malignant anemia was evaluated by measuring four types of outcome.

The results revealed that EPO improved HB levels, based on the change in HB, independent of the follow-up duration. While the overall result was not statistically significant, the finding is supported by previous studies (26,27). The overall result did not reach statistical significance primarily due to the small sample size. Cancer-associated anemia reduced the HCT level below normal, which was reversed by EPO at the short-and long-term follow-ups. With respect to transfusion requirements and the ratio of transfusion, EPO reduced the need for transfusions. The HB levels were increased owing to stimulation by EPO, which improved the patients' anemic state, thereby reducing the demand for transfusions. EPO is a highly glycosylated (40% of total molecular weight) compound with half-life of ~5 h in the blood, which may vary between its endogenous and various recombinant versions (28). Additional glycosylation or other alterations of EPO via recombinant technology increases its stability in blood (thus reducing the frequency of injections required) (29). EPO binds to the EPO receptor on the red cell progenitor surface and activates a Janus kinase 2 signaling cascade. EPO receptor expression is found in a number of tissues, such as bone marrow and peripheral/central nervous tissue. In the bloodstream, red cells themselves do not express EPO receptors, so they are not able to respond to EPO (30). However, indirect dependence of red cell longevity in the blood on plasma erythropoietin levels has been reported, a process termed neocytolysis (31). The majority of anemic patients with cancer of the gastrointestinal tract have iron deficiency due to subclinical blood loss; therefore, an iron supplement has been advocated (32). In particular, anemia is common in patients with colorectal cancer, as they are more likely to experience a loss and malabsorption of iron (33). However, iron supplementation (5,34) has not been found to be able to stimulate erythropoiesis to a sufficient degree to facilitate autologous blood donation or to reduce the need for allogeneic blood transfusions in patients with cancer (32). This may be due to a reticulo-endothelial blockage of iron, characteristic of several other conditions observed in these patients (35). The treatment scheme combining EPO with iron resulted in a more profound HB increase with intravenous iron, which may contribute to a superior optimization of the patient's condition and possibly a decrease in postoperative morbidity (33).

The advantage of the current meta-analysis was that itincluded studies to explain the effect of EPO from four comprehensive aspects ofblood indicators (20). Thestudy had several limitations. Firstly, only a small number of trials met the inclusion/exclusion criteria, and the meta-analysismay have benefitted from more associatedclinical research to support its findings (17). Secondly, certain trials had missing data (36), which was a source of heterogeneity, and the current studywas unable to perform meta-regression for confounding factors.

In conclusion, the currentstudy suggests that EPO reduces cancer-associated anemia as well as improving relevant blood parameters among this patient population.

 Abbreviations EPO

erythropoietin

 HB

hemoglobin

 HCT

hematocrit

 MeSH

medical subject headings

 ITT

intention-to-treat

 MD

mean difference

 NA

not applicable

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Summary of trial identification and selection process.

Forest plot of change in hemoglobin. EPO, erythropoietin; SD, standard deviation; CI, confidence interval.

Forest plot of change in hematocrit. EPO, erythropoietin; SD, standard deviation; CI, confidence interval.

Forest plot of the ratio of transfusion outcome. EPO, erythropoietin; SD, standard deviation; CI, confidence interval.

Forest plot of transfusion requirements outcome. EPO, erythropoietin; SD, standard deviation; CI, confidence interval.

Funnel plot of the ratio of transfusion.

Baseline information of the studies included.

Usage, Dosage, Frequency
First author Year Country Sample (F, I/C) Age (I/C) Criteria of tumor staging Tumor staging (I/C) Tumor location (I/C) HB levels (I/C, g/dl) EPO type Intervention Control ITT/PP (Refs.)
Kettelhack 1998 Germany 48 (27)/54 (32) 71 (53–87)/67 (37–91) NA NA CC 11.5 (10.5–12.5)/12.0 (11.0–13.1) EPO beta EPO 20,000 IU/day Placebo ITT (22)
Scott 2002 USA 29 (13)/29 (11) 67.6±11.0/61.8±10.8 AJCC 1997 1/1, II5/5, III 6/2, IV 17/21 PC, BM 10-13.5 EPO alfa EPO 600 IU/kg/day, 150 mg FeSO4, BID 300 mg/day by FeSO4 PP (23)
Kosmadakis 2003 Greece 31 (16)/32 (13) 67.1±2.1/66.4±2 NA NA Stomach 18%/19%, Colon 53%/52%, Rectum 29%/29% 8.5–13 EPO alfa EPO, 100 mg/day iron Placebo, 100 mg/day iron ITT (24)
Christodoulakis 2005 Greece 67 (37)/68 (40) 71 (36–92)/70 (44–89) Dukes A and B 28/26, C and D 31/32, unknown 8/10 CC 9–12 EPO alfa EPO 300 IU/kg/day, 200 mg/day iron 200 mg/day iron ITT (25)
Christodoulakis 2005 Greece 69 (38)/68 (40) 72.5 (43–91)/70 (44–89) Dukes A and B 35/26, C and D 29/32, unknown 5/10 CC 9–12 EPO alfa EPO 150 IU/kg/day, 200 mg/day iron 200 mg/day iron ITT (25)
Mystakidou 2005 Greece 50 (28)/50 (33) 63 (32–79)/64.5 (41–79) NA NA PC 36%/16%, CC 16%/16%, GS 32%/22%, C: LC 18% 10.15±0.15/9.87±0.50 EPO alfa EPO 40000 IU QD/BID, 200 mg/day iron 200 mg/day iron ITT (26)

AJCC, the American Joint Committee on Cancer; BM, Bone metastases; HB, Hemoglobin; I, Intervening group; C, Control group; CC, Colonic carcinoma; EPO, erythropoietin; F, female; GS, Genital system; ITT, Intention-to-treat; LC, lung cancer; PC, Prostate cancer; PP, Per protocol; NA, Not applicable; TNM, Tumor Node Metastasis; IU, international units; QD, daily; BID, twice daily.