The aim of the present study was to investigate the efficacy and life quality of temozolomide (TMZ) combined with whole-brain radiotherapy (WBRT) for patients with brain metastases (BM) from non-small-cell lung cancer (NSCLC). A total of 77 cases of patients with lung cancer and BM were selected and divided into two groups; the first group was administered WBRT with TMZ, and the second group was administered WBRT with placebo. The efficacy, overall survival (OS) and quality of life (QoL) were then compared between the two groups. There were no significant differences in toxicity between the two groups. However, the first group exhibited an advantage over the second group in terms of objective response and OS (P<0.5). Therefore, WBRT may improve the QoL of patients with BM. TMZ concomitantly with WBRT was well-tolerated and may be recommended for the treatment of BM from NSCLC.
non-small-cell lung cancerbrain metastaseswhole-brain radiotherapytemozolomidequality of lifeIntroduction
Brain metastases (BM) are particularly frequent in lung cancer cases (40–50%), and their presence considerably worsens the prognosis of the patients (1). It has been estimated that 25% of patients with non-small-cell lung cancer (NSCLC) also suffer from BM (2). Patients with BM often experience severe neurological symptoms and a poor quality of life (QoL). The overall survival (OS) of patients with BM from NSCLC is <3–6 months without treatment (3), and the 1-year survival rate is <20% (4). Whole-brain radiotherapy (WBRT) may prolong survival, it is the standard of care for patients with BM, and it is crucial for the control of distant microscopic metastases (5). However, approximately half of patients with BM experience intracranial progression within a few months of RT (6). Chemotherapy has also exhibited limited efficacy in the treatment of BM due to the presence of the blood-brain barrier (7). The efficacy of tyrosine kinase inhibitors (TKIs), such as erlotinib or gefitinib, in central nervous system metastasis is modest, due to the limited cerebrospinal fluid penetration (8,9); therefore, TKIs are generally used only for lung adenocarcinoma with epidermal growth factor receptor mutations (9). Temozolomide (TMZ) is able to cross the blood-brain barrier and has exhibited efficacy against a number of cancers with BM, including NSCLC (10). The efficacy of TMZ in patients with metastatic lung cancer has been previously demonstrated (11,12). In addition to TMZ, certain alkylating agents, such as cisplatin, may exert an effect on BM by crossing the blood-brain barrier (5). TMZ with cisplatin has been confirmed to be an active and well-tolerated combination in patients with BM (13). The present study was designed to assess the efficacy and the associated QoL of WBRT combined with TMZ in patients with BM from NSCLC.
Patients and methodsPatients
Between January 2013 and July 2015, 77 cases of lung cancer patients with BM were selected and divided into two groups (details shown in Table I). The patients were required to meet the following criteria: i) First-time patients with pathologically diagnosed NSCLC and with no other prior tumors, and with a Karnofsky performance status (KPS) score ≥70; ii) BM confirmed by magnetic resonance imaging (MRI) with at least 3 evaluations of cranial lesions and no previously administered cranial RT; iii) 18< age <75 years; iv) no other serious medical conditions, an expected survival >3 months and adequate renal, hepatic and hematological function. All the patients provided written informed consent, and the study protocol was approved by the Ethics Committee of the Qianfoshan Hospital Affiliated to Shandong University (Jinan, China).
Treatment
The first group of patients were administered WBRT (total dose of 30 Gy/10 fractions, 3 Gy/day on days 1–5 weekly) and TMZ (75 mg/m2 p.o. q.d., until the end of WBRT). The second group of patients were administered WBRT, at the same dose as the first group, with placebo (p.o. q.d. until the end of WBRT). Platinum-based chemotherapy was allowed in the two groups following WBRT, under the guidance of the doctors. The first group was administered TMZ 75 mg/m2 p.o. q.d. on days 1–5 combined with chemotherapy, and the second group was administered chemotherapy and placebo simultaneously. Mannitol and dexamethasone were given at the dose required in order to reduce the RT-related side effects. Treatment was continued until unacceptable toxicity occurred (such as grade 4 myelosuppression that did not improve after 1 week, or coma).
Response evaluation
Blood tests were performed once weekly, and a brain MRI was performed within 1 month of RT completion to evaluate the treatment efficacy. Following RT, all patients were evaluated every 2 months if they experienced no headaches or other symptoms. The evaluation included assessment of complications, physical examination and MRI scan of brain. The primary endpoints included objective response (OR), which was evaluated within 1 week after the end of WBRT. Response to treatment in the brain was assessed according to the World Health Organization/Eastern Cooperative Oncology Group criteria, and defined as CR (complete remission), PR (partial remission), SD (stable disease) and PD (progressive disease). OR was defined as a measure of treatment efficacy (CR + PR). The secondary endpoints were overall survival (OS) and QoL. OS was defined as the time from RT initiation to death or the date of the last follow-up. QoL was measured using the Functional Assessment of Cancer Therapy-Brain (FACT-BR, version 4) before and after WBRT. Adverse reactions were evaluated according to the National Cancer Institute Common Terminology Criteria for Adverse Events, version 3.0 (14).
Statistical analysis
Data were analyzed using SPSS 22.0 software (IBM Corp., Armonk, NY, USA). General information on patients, such as OR and toxicity, were described and compared using the Chi-squared test. OS was calculated with the Kaplan-Meier method and the differences were compared using the log-rank test. The difference in QoL between the two groups prior to and following WBRT was compared by the independent samples t-test, and comparison of the QoL difference within each group before and after WBRT was performed using the paired samples t-test. P<0.05 were considered to indicate a statistically significant difference.
ResultsPatients
All the patients completed the treatment successfully. The patient characteristics are listed in Table I. The sample included a total of 49 male and 28 female patients, with a median age of 57 years and a median KPS score of 80 for the first group, and a median age of 61 years and a median KPS score of 80 for the second group. The sex ratios of male to female patients were 1.86 and 1.64, respectively, in the two groups.
Treatment and efficacy
As shown in Table II, all the patients were followed up after the completion of RT. The first group was superior to the second group in terms of OR (P<0.05). A total of 3 patients were lost to follow-up at 6, 8 and 12 months after RT. The median OS was 10 months [95% confidence interval (CI): 8.0–12.0 months] for the first group and 8.5 months (95% CI: 7.3–9.8 months) for the second group. The first group exhibited an advantage in terms of OS when compared with the second group (P<0.05; Fig. 1). There was no significant difference between the two groups before and after RT (Table IV). However, QoL after RT was higher compared with that prior to RT in both groups (group 1, P<0.001; group 2, P<0.001; Fig. 2).
Safety
The toxicities observed are summarized in Table III. There was no significant difference in the side effects between the two groups. Hematological toxicity and vomiting in the first group were more severe compared with those in the second group, but the difference was not statistically significant. Only a few patients in both groups developed grade 3 or 4 toxicities.
Discussion
Lung cancer is the type of cancer that most commonly metastasizes to the brain. The median survival of patients with BM from lung cancer is <3 months (3). There are several treatment options for BM, such as WBRT, stereotactic body radiation therapy (SBRT), surgery and chemotherapy. The efficacy of SBRT and surgery is limited, as they may be applied to single small metastatic lesions, but BM generally manifest as multiple lesions. WBRT has been shown to increase the median survival in a number of trials, and it is considered as the standard treatment for BM (15,16). The use of conventional chemotherapeutic drugs is limited, as it is difficult for these drugs to cross the blood-brain barrier. TMZ, which can penetrate through the blood-brain barrier, has demonstrated efficacy in BM, and may increase the effectiveness of WBRT (17). A meta-analysis including four trials demonstrated that the combination of TMZ and WBRT was superior to RT alone in terms of PR and SD. The combination group also exhibited a mild improvement compared with RT alone in terms of OS. However, the combination group also exhibited a significantly higher incidence of gastrointestinal and grade >3 hematological toxicities compared with the RT alone group (17). Another meta-analysis also demonstrated that concomitant administration of TMZ and RT significantly increased the OR in patients with BM compared with RT alone, but failed to demonstrate a survival advantage (18). A phase 2 trial demonstrated that the OR for TMZ combined with RT was significantly higher compared with that observed with RT alone (P=0.017). The median OS of the combination group was higher compared with the median OS of the RT alone group (8.6 vs. 7.0 months, respectively) (19). In another trial, the addition of TMZ to RT conferred an advantage in terms of PFS and OR (P<0.05), but there was no significant improvement in OS compared with RT alone. There was little difference in QoL between the two groups (20). A trial reported that the OR rate of concomitant WBRT with TMZ was 45%. The median time to progression (TTP) was 9 months and the median OS was 13 months. The analysis of QoL in the trial revealed a high level of satisfaction among patients (21). Addition of TMZ to RT increased OR in patients with BM from NSCLC, as determined in a meta-analysis, but the differences in PFS and OS were not statistically significant (22). The efficacy and safety of TMZ were confirmed in these trials. However, other trials failed to demonstrate any significant improvements in OR or OS with the addition of TMZ to WBRT. A phase 3 trial reported that the combination of TMZ or erlotinib with RT did not improve OS in NSCLC patients with BM. The trial also demonstrated that the OS of erlotinib compared with that of TMZ in combination with RT did not differ significantly (23). Another phase 2 study reported no differences in TTP and OS between the TMZ + RT and the RT alone groups (24), and a phase 3 trial reported that adding TMZ or erlotinib to RT conferred no OS benefit to patients with BM from NSCLC (8). In addition, the benefit of adding TMZ to WBRT has not been confirmed (11). Therefore, the administration of TMZ concomitantly with RT in patients with BM currently remains controversial.
In the present study, we reported an OR rate of 67.5% in the first group, while the OR rate was 43.2% in the second group. WBRT plus TMZ is a well-tolerated treatment with high response rates. In addition to its antitumor effect, TMZ may also increase the sensitivity of tumor cells to RT. The improvement in OS following the addition of TMZ to the treatment regimen may be due to the improved local control of BM. The Graded Prognostic Assessment (GPA) score may be used to evaluate the prognosis of lung cancer. An attempt was made to evaluate the effect of the GPA score on treatment efficacy and patient survival in the two groups, but there was no statistically significant difference. The reason may be that the sample size was limited. In addition, the lack of randomization is also a limitation of the present study, and the results including the value of GPA score require future confirmation by large-sample clinical trials in the future. Based on the results of this trial, TMZ is recommended for concomitant use with WBRT in patients with BM from lung cancer. PFS prolongation in patients with intracranial metastatic tumors is key to improving OS. Previous trials confirmed that TKIs combined with WBRT can prolong the OS when compared with WBRT alone, while other trials reported little benefit in terms of OS (25,26). The reason for this inconsistency may be associated with the fact that the concentration of TKIs in the cerebrospinal fluid is markedly lower compared with their concentration in the serum. A large-sample trial is required to confirm the effect of TKIs on BM. The OS of patients with BM is shortened by treatment limitations, and the QoL of the patients is also becoming increasingly important. The QoL of patients with BM may be evaluated by FACT-BR, which has exhibited good reliability and validity (27). The QoL was not compromised in the TMZ combination group compared with the RT alone group in our trial, which is consistent with previous reports (28). The QoL of patients following RT was higher compared with that prior to RT, which may be due to the reduction of the tumor volume and relief of the peripheral nerve compression symptoms (28). Furthermore, psychological support and nursing interventions may improve QoL (29). The incidence of BM exhibits an increasing trend due to the increased incidence of cancer, but the OS of patients with BM was not significantly prolonged. Treatment of BM represents a major challenge, and there is an urgent need for novel drugs that are able to penetrate the blood-brain barrier.
Acknowledgements
Not applicable.
Funding
This study was supported by the National Natural Science Foundation of China (grant no. 81672974).
Availability of data and materials
The analysed data sets generated during the study are available from the corresponding author on reasonable request.
Authors' contributions
JZ designed this trial and was responsible for the final decision to submit for publication and had the full data of the paper. YL gathered data and wrote the report. ZL and NL helped to gather data. YT performed the literature search. All the authors have read and approved the final version of this manuscript.
Ethics approval and consent to participate
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. The study protocol was approved by the Ethics Committee of the Qianfoshan Hospital Affiliated to Shandong University, and all the patients provided written informed consent.
Consent for publication
All the patients provided written informed consent.
Competing interests
The authors declare that they have no competing interests.
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The OS of the two groups was calculated with the Kaplan-Meier method and the difference in OS between the two groups was compared using the log-rank test. Blue line, OS of the first group; green line, OS of the second group. OS, overall survival.
The difference in each group before and after radiotherapy. Mean score of QoL in the two group and comparison of the QoL difference in each group before and after WBRT was performed using the paired sample t test. The blue column indicates the average score before radiotherapy and the red column indicates the average score after radiotherapy. QoL, quality of life; WBRT, whole-brain radiotherapy.