Introduction
Caveolin-1 (Cav-1) is an important structural and functional component of caveolae, and is known to directly interact via its scaffolding domain with multiple signaling molecules (1). Cav-1 appears to act as a tumor suppressor and an oncogene, depending on the context and type of cancer. Cav-1 reportedly produces inhibitory effects on breast cancer, as it is associated with breast cancer development and progression (2,3). Under normal physiological conditions, Cav-1 is abundantly expressed in breast stromal fibroblasts (4,5). However, Cav-1 expression is reduced in stromal fibroblasts of the breast cancer microenvironment, and negatively correlated with the malignant potential of tumor cells. Breast cancer patients with low or negative Cav-1 expression in stromal fibroblasts often present a low survival rate, whereas the survival rates of those with high stromal Cav-1 expression levels are higher (4,6).
Although the prognostic values of the downregulation of stromal Cav-1 in patients with breast cancer have been reported, the exact mechanism is unclear (7). In order to fully assess the function of Cav-1 as a tumor suppressor, further research into the mechanisms of its expression is required. Additionally, the correlations between Cav-1 expression, tumor stromal fibroblasts and cancer cells must be verified.
Fibroblasts are major stromal cells for cancer and are central to tumorigenesis, tumor growth and metastasis; they secrete multiple factors that may prevent apoptosis, induce proliferation and stimulate tumor angiogenesis (8,9). Thus, a precise understanding of how stromal fibroblasts promote tumor progression is important. Cav-1 downregulation may be a mechanism implicated in the oncogenic transformation of fibroblasts. Decreased expression levels or deleted Cav-1 in fibroblasts can create a tumorigenic microenvironment, but the relevant molecules are not fully clear (10).
Tumor protein 53-induced glycolysis and apoptosis regulator (TIGAR) was discovered in 2005, following p53 activation and detection with microarray analysis (11). The overexpression of TIGAR during cancer development has been noted in various types of tumor. Furthermore, cancer development is often delayed in the case of TIGAR deletion. Recent research has highlighted that the expression and activity of TIGAR can be disengaged from the p53 response, narrowing the focus of its role in cancer development (12). Nevertheless, the activity of TIGAR and the underlying mechanisms of regulation require further investigation to allow for a more complete understanding of its role in tumor pathology.
The present study aimed to clarify the potential molecular mechanism of decreased Cav-1 in promoting tumor growth through an investigation of Cav-1-targeted molecules in tumor stromal fibroblasts and breast cancer cells. Using siRNA, downregulation of the expression of Cav-1 was performed, and the levels of certain growth factors were assessed, including stromal cell-derived factor-1 (SDF-1), epidermal growth factor (EGF), fibroblast-specific protein-1 (FSP-1) and TIGAR. The current study provides evidence for the role of Cav-1 in tumor suppression.
Materials and methods
Cell culture and co-culture
The human skin fibroblast line CCC-ESF-1 (ESF) and human breast cancer cell line BT474 were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). ESF or BT474 cells were cultured in Dulbecco's modified Eagle's medium (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) with 10% fetal bovine serum (GE Healthcare Life Sciences, Logan, UT, USA), 10 µg/ml streptomycin and 100 U/ml penicillin (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C in a humidified atmosphere with 5% CO2. ESF and BT474 cells were co-cultured using polyester Transwell inserts (0.4 µm pore size; Thermo Fisher Scientific, Inc.). Cells cultured on 6-well culture plates were used to detect the expression of proteins. Cells cultured on 24-well culture plates were used to assess levels of reactive oxygen species (ROS), cell proliferation and apoptosis. ESF cells were plated at the bottom of each well of the companion culture plates and allowed to adhere for a minimum of 2 h without apical Transwell inserts. Subsequent to plating, ESF cells were exposed to BT474 cell-conditioned media by placing the BT474 Transwell inserts into the wells previously plated with ESF cells. This method allowed the ESF and BT474 cells to grow in the same medium without direct contact between them. The co-culture models are presented in Fig. 1.
Cav-1 siRNA synthesis and transfection
Cav-1 siRNAs were synthesized by GenePharma Co., Ltd. (Shanghai, China). The following sequences were used: Cav-1 siRNA-1, sense 5′-GCG ACC CUA AAC ACC UCA ATT-3′ and antisense 5′-UUG AGG UGU UUA GGG UCG CTT-3′; Cav-1 siRNA-2, sense 5′-CCU UCA CUG UGA CGA AAUA TT-3′ and antisense 5′-UAU UUC GUC ACA GUG AAG GTT-3′; Cav-1 siRNA-3, sense 5′-GCC GUG UCU AUU CCA UCU ATT-3′ and antisense 5′-UAG AUG GAA UAG ACA CGG CTT-3′; negative control siRNA, sense 5′-GCC GUG UCU AUU CCA UCU ATT-3′ and anti-sense 5′-ACG UGA CA C GUU CGG AGA ATT-3′. ESF-1 cells at 70–80% confluence were transfected with the Cav-1 small interfering RNA or the negative control siRNA by Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. Total RNA and total cellular protein were extracted at 24 and 48 h after transfection, respectively, to verify the effects of the Cav-1 siRNAs.
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
Total RNA was isolated using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.). RNA concentrations were measured using an SMA4000 spectrophotometer (Merinton Instrument, Ltd., Beijing, China) at wavelengths of 260 and 280 nm. The extracted RNA was reverse-transcribed into the cDNA using a RevertAid First Strand cDNA Synthesis kit (Fermentas; Thermo Fisher Scientific, Inc.). qPCR analysis of the cDNA was performed in triplicate using an IQ SYBR Green Supermix (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and Super Real Premix Plus (Tiangen Biotech Co., Ltd., Beijing, China). Relative changes were calculated using the 2−ΔΔCq method. The primer sequences used were as follows: Cav-1, sense 5′-ACGTAGA CTC GGA GGG ACATC-3′ and antisense 5′-GCA GAC AGC AAG CGG TAAA-3′; FSP-1, sense 5′-CCC CAA GAA CAT CCA AAGT-3′ and antisense 5′-TTC AGG AAC AGC CAC CAGT-3′; SDF-1, sense 5′-CAG GTG GTG GCTT AAC AGG-3′ and antisense 5′-AAG AGG AGG TGA AGG CAGTG-3′; EGF, sense 5′-CAA AAC GCCGAAG ACT TACC-3′ and antisense 5′-GAC CAT CCA GAG CCA GACAC-3′; TIGAR, sense 5′-CAG CGG TAT TCC AGG ATTAG-3′ and anti-sense 5′-ACC TTA GCG AGT TTC AGT CAG-3′. The PCR products were analyzed using CFX Manager 1.6 software (Bio-Rad Laboratories, Inc.).
Western blot analysis
Cells were lysed in 10 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP-40, 0.1% sodium dodecyl sulfate (SDS), 1% sodium deoxycholate and protease inhibitor cocktail tablet (Roche Diagnostics, Basel, Switzerland). The protein content in each sample was determined using a Bio-Rad Protein assay kit (Bio-Rad Laboratories, Inc.). Equal amounts of protein were separated by electrophoresis on 7.5~10% SDS-polyacrylamide gels (Beyotime Institute of Biotechnology, Haimen, China). The electrophoresed proteins were transferred to a nitrocellulose membrane (Bio-Rad Laboratories, Inc.). Subsequent to overnight incubation at 4°C in a blocking buffer [5% non-fat dry milk and 0.05% Tween-20 in Tris-buffered saline (TBST)], the membranes were immunoblotted with antibodies against rabbit anti-human polyclonal Cav-1 and mouse anti-human monoclonal TIGAR (cat. nos. sc-894 and sc-377065; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) for 2 h at room temperature. Following three washes with TBST for 5 min each, the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit IgG, sc-2004 and goat anti-mouse IgG, sc-2005; Santa Cruz Biotechnology, Inc.) at room temperature for 1 h. Finally, the membranes were developed using Beyotime ECL Plus (Beyotime Institute of Biotechnology).
Flow cytometry analysis
Cells were cultured for 72 h following transfection with Cav-1 siRNA-2, harvested and then fixed with 4% paraformaldehyde. Following a wash with 0.1% Triton X-100, the cells were resuspended in 5% bovine serum albumin (Beyotime Institute of Biotechnology) and incubated with the relevant primary antibody for 1 h at 4°C. The antibodies against rabbit anti-human polyclonal SDF-1 (cat. no. sc-28876) and rabbit anti-human monoclonal FSP-1 (cat. no. ab124805) were purchased from Santa Cruz Biotechnology, Inc., and Abcam (Cambridge, UK), respectively. The antibody against rabbit anti-human polyclonal EGF (cat. no. BS3549) was procured from Bioworld Technology, Inc. (St. Louis Park, MN, USA). The cells were washed with phosphate-buffered saline (PBS) and then incubated with the appropriate fluorescein isothiocyanate-conjugated secondary antibody (goat anti-rabbit IgG, sc-2012; Santa Cruz Biotechnology, Inc.) for 30 min at 4°C. The cells were extensively washed with PBS and analyzed on an BDAccuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
Enzyme-linked immunosorbent assay (ELISA)
The Cav-1 siRNA-2-transfected cells (ESFsiCav-1 cells) were co-cultured and mono-cultured for 72, 96 or 120 h subsequent to transfection. The culture supernatants were collected and stored at −70°C. The concentrations of growth factors in the supernatants were determined using a Quantikine ELISA kit and a human SDF-1α Quantikine ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer's protocol. Standards and sample proteins were added and incubated at room temperature for 2 h. Subsequent to washing, the conjugate was added to the wells for 2 h at room temperature. The reaction was stopped with a stop solution. The optical density (OD) of each well was measured at 450 nm (SMA4000 spectrophotometer) and values were correlated to the standard curve to determine protein concentration.
Measurement of intracellular ROS production
The intracellular generation of ROS in BT474 cells was detected using the fluorescent probe 2′,7′-dichlorofluorescein-diacetate (DCFH-DA; Santa Cruz Biotechnology, Inc.). BT474 cells were mono- and co-cultured with ESF cells or ESFsiCav-1 cells for 72 h, then incubated with DCFH-DA (20 mM final concentration) at 37°C for 35 min. The fluorescence was analyzed using an FLx800TBID fluorescence reader (BioTek Instruments, Inc., Winooski, VT, USA) using excitation and emission wavelengths of 475 and 525 nm, respectively. The levels of ROS production were expressed in relative fluorescence units (RFU).
Annexin V binding assay
When BT474 cells reached 80–90% confluence, they were collected by trypsinization (Gibco; Thermo Fisher Scientific, Inc.) and centrifugation at 1,200 × g, and washed once in PBS. An annexin V binding assay was performed with 5×105 BT474 cells for each sample, according to the manufacturer's protocol (Oncogene Science; Nuclea Biotechnologies, Inc., Cambridge, MA, USA), followed by a FACScan cytometry analysis. In brief, cells were incubated in 1.5 µl biotin-conjugated annexin V in 0.5 ml binding buffer for 15 min, and then incubated with phycoerythrin-conjugated streptavidin for 5 min. To distinguish the apoptosis from the other types of cell death, 7-amino-actinomycin (7-AAD) was added prior to FACScan detection on a BDAccuri C6 flow cytometer (BD Biosciences).
Cell counting kit-8 (CCK-8) assay
BT474 cells were seeded in 24-well culture plates and ESF or ESFsiCav-1 cells in Transwell inserts in triplicate. The Transwell inserts plated with ESF or ESFsiCav-1 cells were placed into the wells containing BT474 cells. Fresh medium (100 µl) and CCK-8 solution (40 µl; Wuhan Boster Biological Technology, Ltd., Wuhan, China) were added to each well of the culture plates following culture for 24, 48, 72, 96 and 120 h. The plates were then incubated at 37°C for 1 h. A 100-µl aliquot of the reactive solution was removed from each sample and then placed in 96-well culture plates. The OD was measured at 450 nm using an ELx800 microplate reader (BioTek Instruments, Inc.).
Statistical analysis
Data are presented as the mean ± standard deviation. Statistical analysis was conducted using Student's t-test using SPSS 17.0 software (SPSS, Inc., Chicago, IL, USA). P<0.05 was considered to indicate a statistically significant difference.
Results
Interference effects of Cav-1 siRNA on ESF cells
RT-qPCR and western blot analyses were performed to determine the interference effects of Cav-1 siRNA on the ESF fibroblast cell line. The results indicated that the Cav-1 mRNA expression levels in ESF cells were significantly reduced in the siRNA-1, -2 and -3 groups compared with the blank control group at 24 h following transfection with 100 nM Cav-1 siRNA (P<0.05; Fig. 2A). Cav-1 mRNA expression levels following siRNA interference were significantly lower in the siRNA-2 group compared with those in siRNA-1 or -3 groups (P<0.05; Fig. 2A).
Western blot analysis demonstrated that the Cav-1 protein expression levels were significantly reduced in the siRNA-1, -2 and -3 groups compared with those in the blank control group at 48 h subsequent to transfection with 100 nM Cav-1 siRNA (P<0.05; Fig. 2B and C). Cav-1 protein expression in the siRNA-3 group was higher than in the siRNA-1 and -2 groups, however no significant differences were identified. These results indicate the specificity of the siRNA used to target Cav-1. Since the RT-qPCR and western blot results indicated that the siRNA-2 group was the most successful in reducing Cav-1 expression, it was used as the Cav-1-specific interference sequence for the sequential study.
Downregulation of Cav-1 in ESF cells promotes the growth of BT474 cells
CCK-8 assays from 24 to 120 h following mono- or co-culture were performed in the BT474 breast cancer cell line to determine the effects of Cav-1 downregulation on the proliferation and viability of the BT474 cells. The groups did not significantly differ in the observed levels of cell proliferation at 24 h. However, BT474 cell proliferation was significantly greater in the ESFsiCav-1/BT474 co-culture group than in the ESF/BT474 co-culture or BT474 mono-culture groups at 48, 72, 96 and 120 h (P<0.05; Fig. 3A).
Compared with the BT474 control group, the viability of BT474 cells of the ESFsiCav-1/BT474 co-culture group increased by 80% (48 h), 144% (72 h), 111% (96 h) and 82% (120 h) and those of the ESF/BT474 co-culture group increased by 33% (48 h), 68% (72 h), 49% (96 h) and 31% (120 h). The percentage increases in the ESFsiCav-1/BT747 cells were significantly greater than those in the ESF/BT474 cells (Fig. 3B).
To investigate the effect of Cav-1 downregulation on apoptosis in BT474 cells co-cultured with ESFsiCav-1 cells, an annexin V binding assay was performed as the BT474 cells reached 80–90% confluence. A 10-fold reduction in the early apoptosis of BT474 cells in the ESFsiCav-1/BT474 co-culture group was observed, compared with the ESF/BT474 cell co-culture group. Furthermore, a 23-fold reduction in the early apoptotic cells in the ESFsiCav-1/BT474 co-culture group was detected, compared with the BT474 cell mono-culture group (Fig. 3C).
Proliferation of BT474 cells was associated with the increase in levels of SDF-1, EGF and FSP-1 in the ESFsiCav-1 cells
The downregulation of Cav-1 in ESF cells promoted the proliferation and viability of BT474 cells. Therefore, the expression of certain proliferation-associated molecules, including SDF-1, EGF and FSP-1, was investigated. ESFsiCav-1 cells were mono- and co-cultured with BT474 cells and the mRNA and protein expression levels of the target molecules were examined by RT-qPCR and flow cytometry. RT-qPCR assay demonstrated that Cav-1 downregulation significantly increased the mRNA expression levels of SDF-1, EGF and FSP-1 in the ESF cells 48 h subsequent to transfection with Cav-1 siRNA-2. Compared with the mono-culture of ESFsiCav-1, the co-culture of ESFsiCav-1 with BT474 exhibited enhanced SDF-1, EGF and FSP-1 mRNA expression, hence exhibiting a synergistic effect (P<0.05; Fig. 4A).
The flow cytometry results were consistent with the RT-qPCR results. SDF-1, EGF and FSP-1 protein expression levels were increased following Cav-1 downregulation, and were significantly higher in the ESFsiCav-1/BT474 co-culture group, compared with the ESF mono-culture group or ESFsiCav-1 mono-culture group at 72 h after transfection with Cav-1 siRNA-2 (P<0.05; Fig. 4B and C).
The concentrations of these molecules in the culture supernatant were determined using ELISA. The results of ELISA indicated that Cav-1 siRNA transfection increased SDF-1, EGF and FSP-1 production in the supernatant of the ESFsiCav-1/BT474 co-culture at 72, 96 and 120 h compared with the control groups (P<0.05; Fig. 4D–F). These data suggest that SDF-1, EGF and FSP-1 are Cav-1-targeted molecules that promote the proliferation of BT474 cells.
Downregulation of Cav-1 promotes TIGAR expression in BT474 cells, alongside inhibition of apoptosis
Apoptosis of BT474 cells was reduced in the co-culture with ESFsiCav-1 cells. Thus, the effects of the downregulation of Cav-1 on the expression of apoptosis regulators in breast cancer cells were investigated. RT-qPCR was used to measure mRNA levels of TIGAR in BT474 cells 48 h after mono-culture and co-culture. The results demonstrated that the BT474 cells from the ESFsiCav-1/BT474 co-culture group expressed significantly higher levels of TIGAR than the cells from the ESF/BT474 co-culture group and those from the BT474 mono-culture group (P<0.05; Fig. 5A). TIGAR protein expression levels were then assessed using western blot analysis 72 h after mono-culture and co-culture, and the results indicated that the TIGAR protein levels were significantly increased in the ESFsiCav-1/BT474 co-culture group compared with the ESF/BT474 co-culture group or BT474 mono-culture group (P<0.05; Fig. 5B and C). The effects of TIGAR expression on ROS regulation can depend, at least in part, on the cell type and context. To elucidate whether the upregulation of TIGAR impacts on ROS production in BT474 cells, the intracellular generation of ROS in BT474 cells was investigated using the fluorescent probe DCFH-DA. As presented in Fig. 5D, co-culture of BT474 and ESFsiCav-1 cells led to a reduction in the fluorescent signal in these cells, compared with the ESF/BT474 co-culture group (589±50 vs. 1298±115; P<0.05) and the BT474 mono-culture group (589±50 vs. 1560±127; P<0.05). Collectively, these results indicate that TIGAR expression is associated with Cav-1 downregulation, and that the upregulation of TIGAR contributes to the inhibition of BT474 cell apoptosis mediated by Cav-1 downregulation.
Discussion
The results from the present study demonstrated that the downregulation of Cav-1 in fibroblasts led to a significant increase in the expression and secretion of the growth factors, SDF-1, EGF and FSP-1. Furthermore, it upregulated the expression of TIGAR, which may accelerate tumor cell proliferation and suppress tumor cell apoptosis.
Fibroblasts from tumor stroma may be more likely to trigger tumor growth compared with normal stroma. These fibroblasts secrete high levels of growth factors, extracellular matrix components and matrix metalloproteinases, but the relevant factors and components are not fully understood (13). The downregulation or loss of Cav-1 expression in stromal fibroblasts is associated with tumor prognosis (14). It has been indicated that Cav-1 loss in stromal fibroblasts of patients with breast cancer may be used as a predictor of the relapse of breast cancer, lymph node metastasis and tamoxifen resistance (15,16). This has not been associated with the expression of the estrogen receptor (ER), progesterone receptor (PR) or human epidermal growth factor receptor-2 (HER2) (15). In patients with ER−/PR−/HER2− breast cancer or ER−/PR−/HER2− ductal carcinoma, the loss of Cav-1 in stromal fibroblasts has been used as an indicator of unfavorable clinical outcome (4). Cav-1 expression in tumor cells is not correlated with breast cancer prognosis (17). Thus, loss of stromal Cav-1 is a key predictor of a 'lethal' cancer microenvironment.
Loss of stromal Cav-1 is also linked with the poor prognosis of prostate cancer and metastasis of bone and lymph nodes (18); and decreased Cav-1 levels in fibroblasts results in increased levels of myofibroblast markers and extracellular matrix proteins in co-cultured human breast cancer cells with fibroblasts, suggesting that Cav-1 downregulation initiates fibroblast activation in tumorigenesis (19). Myofibroblast markers and glycolytic enzymes were observed to be upregulated in a model of cancer-associated Cav-1-deficient fibroblasts under normoxic conditions (20). However, the mechanisms of phenotype transformation from benign to heterogeneous fibroblasts are unclear. Further investigation is required into the molecules associated with Cav-1 expression and tumor stromal fibroblasts and cancer cells, in order to establish multiple Cav-1-specific therapies and further clarify the mechanisms of Cav-1 in tumor growth.
In the present study, fibroblasts were transfected with synthetic siRNA Cav-1 sequences to determine the effect of the downregulation of Cav-1 on tumor stromal and cancer cells. The results indicated that Cav-1 expression was down-regulated in the Cav-1 siRNA-transfected cells (Fig. 2), thus the Cav-1 siRNA sequences effectively interfered with Cav-1 gene expression. The siRNA-2 exhibited a higher interference efficacy than the siRNA-1 or the siRNA-3. Therefore, siRNA-2 was selected as the Cav-1-specific interference sequence for the current study.
Tumor occurrence and development are strongly associated with stromal microenvironment. Additionally, cancer-associated fibroblasts are the major stromal cells in this microenvironment. These fibroblasts are derived from the transdifferentiation of various cells, including quiescent fibroblasts, epithelial cells, endothelial cells, mesenchymal stem cells and pericytes (21,22). Cancer-associated fibroblasts in direct contact with tumor cells secrete various paracrine factors, synthesize oncogenic components and connect oncogenic signal pathways to promote the development and progression of tumor cells (23). In the present study, the co-culture models of fibroblasts with breast cancer cells were established to simulate the breast cancer microenvironment. The reduced levels of Cav-1 in the co-culture were utilized to investigate the association between Cav-1 and fibroblasts and cancer cells through analyzing the expression of cancer-associated molecules in fibroblasts and breast cancer cells.
SDF-1, also termed CXCL12, is a chemotactic cytokine belonging to the large family of CXC chemokines (24–28). SDF-1 induces cell migration, cell adhesion, neutrophil activation and inflammation. Previous studies have reported that SDF-1 is associated with tumor occurrence, metastasis and growth (24,25,29). Stromal cells are key sources of SDF-1, and an increase in SDF-1 expression may be associated with tumor growth. The recruitment of endothelial progenitor cells by SDF-1 and its direct effect on cancer cells may promote tumor angiogenesis (26,30). In the current study, the Cav-1 siRNA fibroblasts/breast cancer cell co-culture group was the most effective in increasing SDF-1 expression amongst the groups investigated. This suggests that downregulated Cav-1 and the co-culture with breast cancer cells synergistically increased SDF-1 expression in fibroblasts, and the tumor inhibition effect of Cav-1 may be associated with the inhibition of the signaling pathways in which SDF-1 participates.
EGF is a peptide consisting 53 amino acids, with a variety of biological functions. It stimulates epithelial cell motility, and is thus required for re-epithelialization. It is also a major stimulator of fibroblast migration and wound contraction, and is hypothesized to affect cell proliferation, embryo development and tumorigenesis (31–33). The effect of Cav-1 downregulation on EGF expression in fibroblasts was investigated in the present study. Downregulation of Cav-1 significantly upregulated EGF expression in the fibroblasts. This indicates the antagonistic relationship between Cav-1 upregulation and EGF expression. The microenvironment of the co-cultured Cav-1 siRNA fibroblasts with breast cancer cells was able to enhance the expression of EGF.
FSP-1 (also termed S100A4) is implicated in numerous stages of tumor progression, including motility, invasion and apoptosis, however, its function remains uncertain (34,35). A previous study demonstrated that the co-injection of FSP-1+/+ fibroblasts with tumor cells restores tumor development and metastasis in FSP-1−/− animals, whereas co-injection with FSP-1−/− fibroblasts does not (36). The stromal microenvironment can be altered by FSP-1, in order to favor tumor progression. In the current study, the expression of FSP-1 was significantly higher in the Cav-1 siRNA-transfected fibroblasts than in the control-transfected fibroblasts, which suggests that the downregulation of Cav-1 is an upstream event of FSP-1. The Cav-1 siRNA-induced upregulation of SDF-1, EGF and FSP-1 alters the phenotypes of fibroblasts, causing them to become 'reactive'. The microenvironment of reactive fibroblasts is beneficial to tumor growth. The increased concentrations of SDF-1, EGF and FSP-1 in the culture supernatant of Cav-1 siRNA fibroblasts can accelerate the proliferation of tumor cells. The alterations in proliferation of breast cancer cells were consistent with changes in SDF-1, EGF and FSP-1 expression in the current study, which suggests that high expression levels of SDF-1, EGF and FSP-1 can promote breast cancer cell proliferation.
TIGAR may protect cells from ROS-associated apoptosis, and thus, downregulation of the expression of TIGAR may lead to p53-induced cell death (11,37). It has been determined that p53 is not required for TIGAR expression and activity (12). Therefore, in order to identify the function of TIGAR in cancer development, the factors regulating it require further study. The present study identified that the breast cancer cells from the Cav-1 siRNA fibroblasts/breast cancer cell co-culture group presented the highest increase in the expression levels of TIGAR. Downregulation of Cav-1 in fibroblasts influenced the surrounding tumor cells via SDF-1, EGF, FSP-1 and TIGAR. Initially, downregulation of Cav-1 increased the concentrations of the tumor-associated molecules SDF-1, EGF and FSP-1 in tumor stroma. This triggered the accelerated proliferation of tumor cells, which may synergistically influence the expression of TIGAR in cancer cells, suppressing cancer cell apoptosis. The downregulation of Cav-1 in fibroblasts may not produce direct effects in tumor cells. However, the resulting altered stromal microenvironment (with increased expression levels of SDF-1, EGF and FSP-1) demonstrates its importance in tumor suppression.
Cancer cells rapidly proliferate, and TIGAR expression levels are upregulated in cancer cells (38). TIGAR functions to limit ROS, thus protecting cells against ROS-induced death. As demonstrated in the current study, the upregulation of TIGAR expression was accompanied by low levels of ROS. The Cav-1-targeted cascade reactions observed in the present study may be the hallmark of a malignant breast tumor.
In summary, the current study highlighted Cav-1-targeted molecules and their regulatory events, including the regulation of SDF-1, EGF and FSP-1 expression and secretion in stromal fibroblasts. Downregulation of Cav-1 promotes the upregulation of TIGAR expression in breast cancer cells, resulting in cancer cell proliferation and the suppression of cancer cell apoptosis. These results provide novel insight into the tumor-suppressor mechanism of Cav-1, indicating that Cav-1-dependent signaling involves SDF-1, EGF, FSP-1 and TIGAR.