The aim of the present study was to identify polymorphic forms of the nuclear receptor subfamily 3, group C, member 1 (NR3C1) and transforming growth factor β1 (TGF-β1) genes and evaluate their impact on the expression levels of interleukin (IL)-5 and IL-15 in asthma. The study was conducted on a control group consisting of 91 people (54 women and 37 men). The patient group consisted of 130 participants (86 women and 44 men). Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR-high resolution melting (HRM) methods. Interleukin expression was measured by reverse transcription-quantitative polymerase chain reaction. The frequency of the polymorphic forms in the analyzed group were observed to be: Tth111I (rs10052957) controls AA 0.0440, AG 0. 5714, GG 0.3846, patients AA 0.1538/AG 0.4692, GG 0.3769; ER22/23EK (rs6189/rs6190) controls AG 0.0556, GG 0.9444, patients AG 0.0385, GG 0.9615; N363S (rs6195) controls AA 0.6444, AG 0.2667, GG 0.0889, patients AA 0.7846, AG 0.1385, GG 0.0769; BclI (rs41423247) controls CC 0.0879, CG 0.5604, GG 0.3516, patients CC 0.1008, CG 0.5736, GG 0.3256; C-509T (rs1800469) controls TT 0.0805, CT 0.6322, CC 0.2874, patients TT 0.1102, CT 0.5669, CC 0.3228. The results indicated that the C-509T single nucleotide polymorphism (SNP) of the TGF-β1 gene contributed to an increase in the IL-5 mRNA expression levels. The GG genotype of the N363S SNP of the NR3C1 gene was observed to result in an increase in the expression levels of IL-15. The present study indicated that the selected SNPs of the NR3C1 and TGF-β1 genes demonstrate a regulatory effect on the expression of IL-5 and IL-15. Therefore, genetic variation affects inflammation in asthma and the clinical course of the disease.
Asthma is a heterogenous disorder, characterized by chronic inflammation of the respiratory tract. Numerous genes and their polymorphic forms are involved in the pathogenesis of this disease. It is estimated that 35–70% of cases of asthma and allergy are genetically conditioned (1,2). The following genes serve a particularly important role in the pathophysiological mechanisms of allergic diseases and asthma: Growth factors [e.g. transforming growth factor-β, (TGF-β)], cytokines regulating immunological processes [interleukins, e.g. interleukin (IL)-5, IL-15] and those which condition a positive response to glucocorticosteroid (GC) therapy [nuclear receptor subfamily 3, group C, member 1 (NR3C1)] (2–7).
The NR3C1 gene is located on the long arm of chromosome 5 (5q31–q32) and encodes the GC receptor (GR). The GR consists of 5 structural domains (A/B, C, D, E and F), which exhibit biological function: Activation function 1 (AF1); DNA-binding domain (DBD); nuclear localization sequence (NLS); ligand binding domain (LBD); and AF2. The occurrence of polymorphisms in the NR3C1 gene modifies the activity of the gene regulatory parts, which affects the function of the promoter and coded protein (8–11). Tth111 I (rs10052957), ER22/23EK (rs6189/rs6190), N363S (rs6195) and BclI (rs41423247) single nucleotide polymorphisms (SNPs) of the NR3C1 gene increase the risk of asthma, affect the clinical course of the disease and condition the response to GC anti-inflammatory treatment (level of asthma control, steroid dependence or steroid resistance) (12–15). Following the binding of the GC ligand, the GR becomes an active transcription factor, which inhibits the expression (transrepression) of certain genes, such as IL-5 (2,16–19). In the transactivation mechanism, an increased production of anti-inflammatory proteins is observed. In addition, GCs increase the production and release of IL-15 (2,20).
IL-5 is produced by activated T lymphocytes and macrophages, and in asthma promotes the differentiation, migration and activation of eosinophils, and prolongs their survival in sites of allergic inflammation. The occurrence of IL-5 manifests with bronchial hyperreactivity, clinical symptoms and bronchial obstruction, as a result of inflammatory mediators released from eosinophils, such as leukotrien C4) (21–24).
IL-15 serves a vital role in the maturation of natural killer (NK) cells. It stimulates antibody-dependent cytotoxicity, differentiates monocytes to dendritic cells with a Langerhans cell phenotype, stimulates T lymphocyte proliferation and migration, and regulates inflammatory responses to the production of cytokines and chemokines (20,25,26). In a comparison of a group of patients with asthma treated with GCs with a control group, it was indicated that the sputum of the patients with asthma contained higher levels of IL-15, which suggests a significant role of this interleukin in the regulation of immune and inflammatory responses in asthma (20,26).
There are three different human isoforms of TGF-β, which are located in the following chromosomal loci: TGF-β1-19q13, TGF-β2-1q41 and TGF-β3-14q24 (27–29). Asthma is associated with elevated levels of TGF-β1 and TGF-β2. TGF-β1 is expressed on the vascular endothelium, and in smooth muscles, fibroblasts and platelets. It regulates the growth of eosinophils and lymphocytes in response to exposure to allergens. TGF-β promotes the growth of smooth muscle, subepithelial fibrosis, increases production of the extracellular matrix and promotes fibroblast activation. In addition, it regulates allergic inflammation in asthma and affects airway remodeling (30–32). TGF-β induces IL-5 in the proapoptotic cosignaling mechanism (33,34). It leads to the suppression of activated peripheral blood eosinophils and tissue eosinophils via the inhibition of Lyn kinase, mitogen-activated protein kinase (MAPK), Janus kinase 2 and signal transducer and activator of transcription 1 nuclear factor (33–39). TGF-β regulates IL-15 expression, with TGF-β observed to increase the expression levels of IL-15. In a previous study, following the application of siRNA, mothers against decapentaplegic (Drosophila) and SMA (Caenorhabditis elegans) homolog 3 (Smad3), however, not Smad2 was observed to be involved in the induction of IL-15 mRNA induction by TGF-β (40). In addition to the Smad2/3 pathway, TGF-β activated numerous kinases, MAPK and c-Jun N-terminal kinase, and the phosphoinositide 3-kinase-protein kinase B signaling pathway (40). However, one report indicated that TGF-β1 inhibits the production of IL-15 (41). SNPs in the TGF-β1 gene including C−509T (rs1800469), C+466T (rs200482214) and T+869C (rs1800470) influence the expression levels of TGF-β1 and immunoglobulin E (IgE), bronchial remodeling, the risk of asthma and its clinical control (42–48).
The aim of the present study was to identify polymorphic forms of the NR3C1 and TGF-β1 genes and evaluate their effect on the expression levels of IL-5 and IL-15 in asthma.
Materials and methodsStudy participants
The current study was approved by the local ethics committee (Consent of Research Review Board, Medical University of Lodz, Poland, No RNN/133/09/KE). Study participants were volunteers and written informed consent was obtained from every patient prior to enrolment in the study.
Asthma diagnosis was established according to the Global Initiative For Asthma (GINA) recommendations (www.ginasthma.org), based on clinical asthma symptoms and a lung function test. The level of asthma severity and control was determined on the basis of GINA Report Guidelines. The patients medical history, including gender, obesity, tobacco smoking, duration of bronchial asthma, allergy to house dust mites, animal fur, mould spores, cockroach allergens, hypersensitivity to non-steroidal anti-inflammatory drugs was collected, in addition to objective examinations (spirometry tests). The results of pulmonary function tests and allergological tests were obtained from the individual medical records of the patients. If no spirometry or allergological test results were available, such examinations were performed during the recruitment visit. Subjects demonstrating clinically significant exacerbations, using drugs that may induce resistance to glucocorticoids (e.g. rifampicin, phenobarbital, phenytoin and effedrine), subjects with signs of viral infections (either generalized or affecting the respiratory tract), in addition to subjects failing to comply with the doctor's recommendations, were excluded from the patient group. The control group consisted of healthy adults, who met the following criteria: No history or symptoms of either bronchial asthma or other pulmonary diseases, no history or symptoms of allergy, no history or symptoms of atopic dermatitis, no history or symptoms of hypersensitivity to aspirin, negative results of skin tests for 12 common allergens and no first-degree relatives with bronchial asthma or atopic disorders. Spirometry tests were conducted in the Specialist Outpatient Clinic of Pulmonary Diseases and Allergology of the Norbert Barlicki University Clinical Hospital (Lodz, Poland) according to European Respiratory Society/American Thoracic Society standards (49), and allergological tests according to the guidelines of the European Academy of Allergy and Clinical Immunology (7,13,15,28).
The study was conducted on 221 participants. The control group consisted of 91 people [54 women (59.34%) and 37 men (40.66%)], with an age range of 21–80 years old. The mean age in the control group was 46.96±16.42 years, with a median of 48.00 years. The patient group consisted of 130 participants [86 women (66.15%) and 44 men (33.85%)], with an age range of 22–78 years old. The mean age in the patient group was 50.24±14.55 years, with a median of 52.00 years. Non-severe asthma was diagnosed in 75 patients (57.69%), whereas 55 patients (42.31%) had a severe form of the disease. Comprehensive comparison of spirometric parameters of the analyzed groups is presented in Table I.
The study participants were stratified and subjected to statistical analysis. The genotyping was performed by two blinded investigators.
SNP analysis
Venous blood samples were collected from the participants into tripotassium ethylenediaminetetraacetic acid tubes (Sarstedt AG & Co., Nümbrecht, Germany). DNA was obtained from the peripheral blood leukocyte fraction. The genetic material was isolated using a QIAamp DNA Blood Mini kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer's instructions. The investigated polymorphisms were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR-high resolution melting (HRM) methods, as described previously (13,50–53). Table II presents a description of the applied genotyping techniques.
A total of 10 µg total RNA was extracted from the peripheral blood lymphocytes using an RNA extraction reagent, (TRI Reagent® Solution; Ambion; Thermo Fisher Scientific, Inc., Waltham, MA, USA), according to the standard of the acid guanidinium phenol-chloroform method (54). The extracted RNA was analyzed by agarose gel (8%) electrophoresis and only cases with preserved 28S, 18S and 5S ribosomal RNA bands indicating good RNA quality were used in the study. Total RNA was digested with DNase (Gibco; Thermo Fisher Scientific, Inc.) at room temperature for 15 min. The amount of purified RNA was determined using spectrophotometry at 260 nm in a Nanodrop ND-100 analyser (Nanodrop; Thermo Fisher Scientific, Inc., Wilmington, DE, USA). The purity was verified according to the ratio of 260/280 nm measurements; values between 1.8 and 2.1 indicated that the quality of the obtained RNA was optimal and suitable for RT-qPCR (7,28,54). Reverse transcription of 1 µg RNA was performed using an AccuScript PfuUltra II RT-PCR kit (Agilent Technologies, Santa Clara, CA, USA). The cDNA was subjected to qPCR using gene specific primers for IL-5, IL-15 and GAPDH using EvaGreen® Master Mix for RT-qPCR (Biotium, Inc., San Francisco CA, USA) and Phusion® High-Fidelity DNA Polymerase (Thermo Fisher Scientific, Inc.). Table III presents the primer sequences used for RT-qPCR. An Agilent Mx3000P qPCR system (Agilent Technologies, Inc., Santa Clara, CA, USA) was used for the IL-5 and IL-15 RT-qPCR reaction. Bands were visualized using ImageMaster (Pfizer, New York City, NY, USA). For each sample, the CQ (quantification cycle) values were calculated using Mx-Pro software (Agilent Technologies, Inc.). The RT-qPCR amplification of the IL-5 and IL-15 genes was compared with the levels of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a house-keeping reference gene, and the ΔCQ values were determined (ΔCQ=CQ, GENE−CQ, GAPDH). The RT-qPCR data was automatically calculated with the data analysis module. The results were analyzed with the application of the 2−ΔΔCT method. Validation of PCR efficiency was performed with a standard curve (7,28,54–56).
Statistical analysis
Nominal variables are presented as percentages, and continuous variables are presented as the mean ± standard deviation or the median and the interquartile range (IQR). The Yate's chi-square test and Fisher-Freeman-Halton exact test were used to test the associations. The continuous variables were analyzed using the Mann-Whitney U test or analysis of variance depending on the number of groups being compared. Correlation coefficients were calculated using the Spearman's rank correlation. To test whether the genotype frequencies of the SNPs were consistent with the Hardy-Weinberg equilibrium, Michael H. Court's online calculator was used: http://www.tufts.edu/~mcourt01/Documents/Court%20lab%20-%20HW%20calculator.xls. P<0.05 was considered to indicate a statistically significant difference. Statistica software, version 10.0 (Statsoft, Inc., Tulsa, OK, USA) was used for statistical analysis.
Results
Different IL-5 mRNA expression levels were observed in the control and patient groups, with a median of 1.46 (patients) (IQR, 0.16–5.34) compared with 0.18 (controls) (IQR, 0.32–1.66; P<0.001). In addition, the IL-15 mRNA expression levels in the patient and control groups were significantly different, with a median of 0.60 (patients) (IQR, 0.25–1.09) compared with 0.27 (controls) (IQR, 0.04–0.75; P<0.01).
It was observed that no SNPs of the NR3C1 gene significantly correlated with the altered levels of IL-5, in which the highest levels of IL-5 mRNA expression were observed (Fig. 1). In the NR3C1 gene, the most common polymorphism observed in Tth111I was the AG genotype (69.23% controls, 62.00% asthmatic patients), in ER22/23EK was the GG genotype (94.74% controls, 97.96% asthmatic patients), in N363S was the AA genotype (78.95% controls, 82.00% asthmatic patients) and in BclI was the CG genotype (48.72% controls, 61.22% asthmatic patients). Notably, the AA polymorphic form was not observed for ER22/23EK. In the TGF-β1 gene, three C-509T polymorphic forms (TT, CT and CC) were observed, with the most common genotype being CT (66.67% controls, 61.22% asthmatic patients). The risk factor contributing to an increase in IL-5 mRNA expression was TT in the C-509T SNP of the TGF-β1 gene (10.26% controls, 8.16% asthmatic patients). Expression of the TT allele increased the risk of higher IL-5 mRNA expression by 44% in the group of asthmatic patients. The polymorphic forms of the TGF-β1 gene, C+466T and T+869C, occurred only in one genotype form, TT (100%), both in the control group and in patients with asthma. However, these polymorphisms did not affect the expression levels of IL-5 mRNA in patients with asthma (Table IV).
It was observed that only one of the four NR3C1 SNPs was significantly associated with the altered IL-15 mRNA expression levels (Fig. 2). The N363S SNP occurred in three polymorphic forms, with the most common genotype being AA (64.44% controls, 78.46% asthmatic patients). The least frequently observed genotype, GG (8.89% controls, 7.69% asthmatic patients), contributed to a significant increase in the level of IL-15 mRNA expression, by 23% in the group of patients with asthma. The remaining NR3C1 SNPs did not affect the level of IL-15. The AG heterozygote was the most common form of Tth111I (57.14% controls, 46.92% asthmatic patients), and in ER22/23EK was the GG homozygote (94.44% controls, 96.15% asthmatic patients). The AA genotype of the ER22/23EK SNP was not observed in either the patient or control group. The most commonly observed genotype in BclI was the CG heterozygote (56.04% controls, 57.36% asthmatic patients). None of the TGF-β1 SNPs altered the IL-15 mRNA expression levels. The C-509T SNP occurred in three polymorphic forms, of which the CT heterozygote was the most common (63.22% controls, 56.69% asthmatic patients). The remaining two SNPs of the TGF-β1 gene, C+466T and T+869C, were observed only in the TT homozygote form (100%) in the control and patient groups, and no other polymorphic forms, such as CT or CC, were observed. In addition, the TGF-β1 SNPs did not affect the IL-15 mRNA expression levels (Table V).
Discussion
Despite allergy being the predominant cause of asthma, certain genetic factors interacting with environmental determinants additionally contribute to the occurrence of the disease. From a clinical perspective, of the genes associated with allergic diseases and asthma, the most important are those which are associated with the function of Th2 lymphocytes, epithelial cells, lung function, bronchial remodeling and the severity of asthma (2). The NR3C1 and TGF-β1 genes may be considered to be such genes.
The role of SNPs in the pathogenesis of asthma is currently under investigation, and there are numerous studies on this issue, performed on populations of various types and sizes, however, not all findings are replicable. Notably though, numerous studies have reported the functional role of the NR3C1 and TGF-β1 genes in asthma (7,13,15,28,50–52). With regards to basic and clinical studies, it is important to know to what extent these SNPs affect the signaling pathways regulated by the NR3C1 and TGF-β1 genes in asthma.
In the present study, the effect of SNPs in the NR3C1 and TGF-β1 genes on interleukin expression was investigated in healthy controls and patients with asthma. The N363S (rs6195) SNP of the NR3C1 gene was observed to have an effect on IL-15 expression, in addition to the C-509T (rs1800469) SNP of the TGF-β1 gene which altered IL-5 expression.
The N363S SNP is localized in exon 2 of the NR3C1 gene. It changes nucleotide sequence AAT to AGT, which results in a conversion of asparagine into serine at codon 363 of the GR (57–59). The N363S results in structural alterations in the A/B region of the GR and functional changes within the AP-1 functional domain, and modulates a number of protein regulation systems (57–59). N363S inhibits the activity of nuclear factor-κB, stimulates the production of inhibitory-κB and interferes with the suppression of IL-2. In addition, N363S has an influence on the level of GR phosphorylation (57–59). The GR-N363S is characterized with an increased ability to transactivate genes encoding proteins synthesized in cellular response to GCs (13,50–52). The present study indicated that the N363S SNP can additionally regulate IL-15 expression in patients with asthma. The product of the expression of the GG allele of this SNP contributed to a significant increase in IL-15 mRNA level, by 23%. Thus, carriers of the GG genotype of the N363S of the NR3C1 gene demonstrate a higher risk of inflammation, as regulated by IL-15 in asthma. The decreased frequency of the mutated G allele in the asthmatic patient group (controls GG=8.89% compared with patients GG=769%) correlated with a reduced response to GC therapy and elevated level of IL-15 mRNA. In vivo studies have indicated that the N363S SNP is associated with an increased sensitivity to GCs (57). In the present study, it was noted that the carriers of the N363S SNP appeared to be clinically healthy and more sensitive to treatment with exogenous GCs. In a previous study, an increased frequency of the occurrence of the A allele at the cost of the G polymorphic allele in the analyzed N363S SNP was statistically correlated with a higher incidence of a more severe form of asthma and the inability to reduce its symptoms with the administration of anti-inflammatory drugs (60). This is a potential mechanism which may account for the increase observed in IL-15 expression in the patients with asthma treated with GCs (60).
The C-509T (allele C/T) SNP, located in the promoter of the TGF-β1 gene, serves a crucial role as it increases the expression of TGF-β1 and IgE. Thus, it is an important factor associated with bronchial remodeling and the development of asthma (46,47). The C-509T SNP is a regulator of the expression of TGF-β1, and carriers of this SNP have been reported to exhibit increased expression of TGF-β1, which, in eosinophils, acts as a negative regulator of eosinophil survival (36). Eosinophils are destroyed by apoptosis when the levels of eosinophil-activating cytokines, such as granulocyte-macrophage colony-stimulating factor and IL-5, are insufficient. TGF-β1 blocks the activity of IL-5, which is unable to prevent apoptosis of eosinophils (36). However, increased expression of TGF-β1 and increased eosinophil accumulation are observed in sites of allergic inflammation in patients with asthma; in addition, eosinophils themselves can produce TGF-β1 (36). These paradoxical observations suggest that eosinophils may have aberrant TGF-signaling, resulting in partial or complete TGF-resistance (36). In the present study, by increasing TGF-β1, the C-509T SNP contributed to an increase in IL-5 expression and exacerbation of the inflammation in the bronchi of patients with asthma. The product of the two TT alleles appeared to increase the risk of IL-5-induced inflammation by 44%. With regards to the remaining TGF-β1 SNPs, the present study did not indicate any correlation between the gene and IL-15 expression. In order to confirm these results, it studies investigating the expression of TGF/Smad signaling pathway will be required, in particular to determine Smad2/3 and Smad4 levels, as these cytokines serve a crucial role in stimulating the synthesis of fibronectin, proteoglycans and type I and III collagen. In addition, these cytokines increase eosinophil chemotaxis following allergen exposure in the bronchi of patients with asthma.
The present study indicated associations, however, further studies are required based on functional models of the investigated proteins. Clinical observations based on the observations of the present study require further experimental studies to confirm these phenomena.
The present study is the first, to the best of our knowledge, to analyze the functional impact of NR3C1 and TGF-β1 SNPs on interleukin expression in patients with asthma. A correlation between the C-509T SNP of the TGF-β1 gene and IL-5 mRNA expression was demonstrated, in addition to a correlation between the N363S SNP of the NR3C1 gene and IL-15 expression. This observation is important because IL-5 has an influence on the level of eosinophils, bronchial hyperreactivity and obstruction in addition to the clinical symptoms of asthma. IL-15 is an important regulator of the immune response in this disease.
The current study provides the clinical basis for performing the genetic screening of patients. This may enable the implementation of methods to prevent asthma development in particular patient groups (such as asthma endotype). Thus, the early identification of patients demonstrating a risk of more severe course of asthma (carriers of certain SNPs) will be possible, in addition to the introduction of an intensive anti-inflammatory therapy (GCs) earlier, which may prevent disease progression, exacerbations and bronchial remodeling.
Acknowledgments
The authors would like to thank Ms. Joanna Molinska, MA for assistance with administration and Ms. Beata Małachowska, MD for assistance with statistical analysis. Ms. Beata Małachowska was supported by the TEAM project financed by the Innovative Economy Operational Program and coordinated by the Foundation for Polish Science. The authors would like to thank Mr. Grzegorz Uraziński, MSc Eng, for the preparation of the figures and assistance in graphic rendering of research materials. The authors would additionally like to thank all those who provided assistance during the completion of the study. The present study was funded by a grant from the Clinic of Internal Medicine, Asthma and Allergies, II Chair of Internal Medicine, Medical University of Lodz, Poland (grant no. 503/1-095-03/503-01).
ReferencesGajewskiPMejzaFNiżankowska-MogilnickaERozpoznanie i leczenie astmy u dorosłych. Podsumowanie wytycznych GINA 2 014Med Prakt92014In PolishFalAAlergia, Choroby Alergiczne, AstmaMedycyna PraktycznaKraków2010In PolishGreenfederSUmlandSPCussFMChapmanRWEganRWTh2 cytokines and asthma. The role of interleukin-5 in allergic eosinophilic diseaseRespir Res27179200110.1186/rr411168686859571HolgateSTPathogenesis of asthmaClin Exp Allergy38872897200810.1111/j.1365-2222.2008.02971.x18498538BroideDHImmunologic and inflammatory mechanisms that drive asthma progression to remodelingJ Allergy Clin Immunol121560570200810.1016/j.jaci.2008.01.031183288872386668TorregoAHewMOatesTSukkarMFan ChungKExpression and activation of TGF-beta isoforms in acute allergen-induced remodelling in asthmaThorax62307313200710.1136/thx.2006.063487172513171892798PanekMPietrasTFabijanAZiołoJWieteskaŁMałachowskaBFendlerWSzemrajJKunaPThe NR3C1 glucocorticoid receptor gene polymorphisms may modulate the TGF-beta mRNA expression in asthma patientsInflammation3814791492201510.1007/s10753-015-0123-325649164ItoKChungKFAdcockIMUpdate on glucocorticoid action and resistanceJ Allergy Clin Immunol117522543200610.1016/j.jaci.2006.01.03216522450LeungDYBloomJWUpdate on glucocorticoid action and resistanceJ Allergy Clin Immunol111322200310.1067/mai.2003.9712532089KinoTDe MartinoMUCharmandariEMiraniMChrousosGPTissue glucocorticoid resistance/hypersensitivity syndromesJ Steroid Biochem Mol Biol85457467200310.1016/S0960-0760(03)00218-812943736NicolaidesNCGalataZKinoTChrousosGPCharmandariEThe human glucocorticoid receptor: Molecular basis of biologic functionSteroids75112201010.1016/j.steroids.2009.09.0022813911HawkinsGAAmelungPJSmithRSJongepierHHowardTDKoppelmanGHMeyersDABleeckerERPostmaDSIdentification of polymorphisms in the human glucocorticoid receptor gene (NR3C1) in a multi-racial asthma case and control screening panelDNA Seq15167173200410.1080/1042517041000170451715497438PanekMPietrasTFabijanAMiłanowskiMWieteskaLGórskiPKunaPSzemrajJEffect of glucocorticoid receptor gene polymorphisms on asthma phenotypesExp Ther Med55725802013234076533570225de LangePKoperJWHuizengaNABrinkmannAOde JongFHKarlMChrousosGPLambertsSWDifferential hormone-dependent transcriptional activation and -repression by naturally occurring human glucocorticoid receptor variantsMol Endocrinol1111561164199710.1210/mend.11.8.99499212062PietrasTPanekMTworekDOszajcaKWujcikRGórskiPKunaPSzemrajJThe BclI single nucleotide polymorphism of the human glucocorticoid receptor gene h-GR/NR3C1 promoter in patients with bronchial asthma: Pilot studyMol Biol Rep3839533958201110.1007/s11033-010-0512-53115143GrzankaAJarzabJNongenomic effects of glucocorticoidsPneumonol Alergol Pol773873932009In PolishBarnesPMolecular mechanisms of glucocorticoid action in asthmaPulm Pharmacol Ther10319199710.1006/pupt.1997.00749344828BarnesPGlucocorticosteroidsAsthma: Basic Mechanisms and Clinical ManagementBarnesPRodgerIThompsonN3rd EditionAcademic PressCambridge, MA725766199810.1016/B978-012079027-2/50122-2NewtonRBarnesPAdcockITranscription FactorsAsthma: Basic Mechanisms and Clinical ManagementBarnesPRodgerIThompsonN3rd EditionAcademic PressCambridge, MA459474199810.1016/B978-012079027-2/50107-6Komai-KomaMMcKayAThomsonLMcSharryCChalmersGWLiewFYThomsonNCImmuno-regulatory cytokines in asthma: IL-15 and IL-13 in induced sputumClin Exp Allergy3114411448200110.1046/j.1365-2222.2001.01174.x11591195AraiKILeeFMiyajimaAMiyatakeSAraiNYokotaTCytokines: Coordinators of immune and inflammatory responsesAnnu Rev Biochem59783836199010.1146/annurev.bi.59.070190.0040311695833BousquetJChanezPLacosteJYBarnéonGGhavanianNEnanderIVengePAhlstedtSSimony-LafontaineJGodardPEosinophilic inflammation in asthmaN Engl J Med32310331039199010.1056/NEJM1990101132315052215562CoyleAJAckermanSJIrvinCGCationic proteins induce airway hyperresponsiveness dependent on charge interactionsAm Rev Respir Dis147896900199310.1164/ajrccm/147.4.8968385431Tomasiak-ŁozowskaMMBodzenta-ŁukaszykATomasiakMSkiepkoRZietkowskiZThe role of interleukin 13 and interleukin 5 in astmaPostepy Hig Med Dosw (Online)641461552010BierbaumSNickelRZitnikSAhlertILauSDeichmannKAWahnUHeinzmannAConfirmation of association of IL-15 with pediatric asthma and comparison of different controlsAllergy61576580200610.1111/j.1398-9995.2006.01059.x16629787JachnikMSzczepankiewiczABręborowiczAInfluence of IL15 gene polymorphism on the course of bronchial asthma in childrenAlergia Astma Immunologia162002042011LetterioJJRobertsABTGF-beta: A critical modulator of immune cell functionClin Immunol Immunopathol84244250199710.1006/clin.1997.44099281382PanekMPietrasTSzemrajJFabijanAKunaPIdentification and association of TGFβ-1 expression in patients with asthma in a Polish population-Lodz metropolitan area studyInt J Biochem Mol Biol467742013Ten DijkePGeurtsVanKesselAHFoulkesJGLe BeauMMTransforming growth factor type beta 3 maps to human chromosome 14, Region q23–q24Oncogene372172419882577876WahlSMTransforming growth factor-beta: Innately bipolarCurr Opin Immunol195562200710.1016/j.coi.2006.11.008BottomsSEHowellJEReinhardtAKEvansICMcAnultyRJTgf-beta isoform specific regulation of airway inflammation and remodelling in a murine model of asthmaPLoS One5e9674201010.1371/journal.pone.0009674203001912837347BosséYStankovaJRola-PleszczynskiMTransforming growth factor-beta1 in asthmatic airway smooth muscle enlargement: Is fibroblast growth factor-2 required?Clin Exp Allergy40710724201010.1111/j.1365-2222.2010.03497.x20447083XieQShenZJOhJChuHMalterJSTransforming growth factor-β1 antagonizes interleukin-5 pro-survival signaling by activating calpain-1 in primary human eosinophilsJ Clin Cell Immunol Suppl12011PardaliKMoustakasAActions of TGF-beta as tumor suppressor and pro-metastatic factor in human cancerBiochim Biophys Acta177521622007ShenZJEsnaultSRosenthalLASzakalyRJSorknessRLWestmarkPRSandorMMalterJSPin1 regulates TGF-beta1 production by activated human and murine eosinophils and contributes to allergic lung fibrosisJ Clin Invest1184794902008181884562176187KanzakiMShibagakiNHatsushikaKMitsuiHInozumeTOkamotoADobashiYOgawaHShimadaSNakaoAHuman eosinophils have an intact Smad signaling pathway leading to a major transforming growth factor-beta target gene expressionInt Arch Allergy Immunol142309317200710.1159/000097500AlamRForsythePStaffordSFukudaYTransforming growth factor beta abrogates the effects of hematopoietins on eosinophils and induces their apoptosisJ Exp Med17910411045199410.1084/jem.179.3.104181136722191410PazdrakKJustementLAlamRMechanism of inhibition of eosinophil activation by transforming growth factor-beta. Inhibition of Lyn, MAP, Jak2 kinases and STAT1 nuclear factorJ Immunol1554454445819957594607GoplenNGorskaMMStaffordSJRozarioSGuoLLiangQAlamRA phosphosite screen identifies autocrine TGF-beta-driven activation of protein kinase R as a survival-limiting factor for eosinophilsJ Immunol18042564264200810.4049/jimmunol.180.6.425618322238ZhaoJLiPGaoSEffect of TGF-beta1 on the expression of IL-12, IL-15, IL-18, IL-4 and IL-10 in heart transplantation rejection in ratsJ Huazhong Univ Sci Technolog Med Sci27643645200710.1007/s11596-007-0605-0GodoyPWengHLBachmannABulanovaEBulfone-PausSDooleySInterleukin-15 expression is induced by TGF-beta in hepatocytes: A new role for IL-15 in liver disease?Z Gastroenterol462432009MakJCLeungHCHoSPLawBKHoASLamWKIpMSChan-YeungMMAnalysis of TGF-beta (1) gene polymorphisms in Hong Kong Chinese patients with asthmaJ Allergy Clin Immunol1179296200610.1016/j.jaci.2005.08.04916387590GraingerDJHeathcoteKChianoMSniederHKempPRMetcalfeJCCarterNDSpectorTDGenetic control of the circulating concentration of transforming growth factor type beta1Hum Mol Genet89397199910.1093/hmg/8.1.939887336HobbsKNegriJKlinnertMRosenwasserLJBorishLInterleukin-10 and transforming growth factor-beta promoter polymorphisms in allergies and asthmaAm J Respir Crit Care Med15819581962199810.1164/ajrccm.158.6.98040119847292PulleynLJNewtonRAdcockIMBarnesPJTGFbeta1 allele association with asthma severityHum Genet109623627200110.1007/s00439-001-0617-ySilvermanESPalmerLJSubramaniamVHallockAMathewSValloneJFaffeDSShikanaiTRabyBAWeissSTShoreSATransforming growth factor-beta1 promoter polymorphism C-509T is associated with asthmaAm J Respir Crit Care Med169214219200410.1164/rccm.200307-973OCNagpalKSharmaSB-RaoCNahidSNiphadkarPVSharmaSKGhoshBTGFbeta1 haplotypes and asthma in Indian populationsJ Allergy Clin Immunol115527533200510.1016/j.jaci.2004.11.04815753900BuckovaDIzakovicováHolláLBenesPZnojilVVáchaJTGF-beta1 gene polymor phismsAllergy5612361237200110.1034/j.1398-9995.2001.00373.x11736765MillerMRHankinsonJBrusascoVBurgosFCasaburiRCoatesACrapoREnrightPvan der GrintenCPGustafssonPStandardisation of spirometryEur Respir J26319338200510.1183/09031936.05.0003480516055882PietrasTPanekMKuprys-LipinskaIOszajcaKWujcikRKunaPGorskiPSzemrajJFrequencies of BclI, E22E, and N363S of h-GR/NR3C1 restriction fragment length polymorphisms of glucocorticoid receptor gene in Polish adult populationMed Sci Monit16CR475CR479201020885351PanekMPietrasTAntczakAFabijanAPrzemęckaMGórskiPKunaPSzemrajJThe N363S and I559N single nucleotide polymorphisms of the h-GR/NR3C1 gene in patients with bronchial asthmaInt J Mol Med30142150201222469783PanekMPietrasTAntczakAGórskiPKunaPSzemrajJThe role of functional single nucleotide polymorphisms of the human glucocorticoid receptor gene NR3C1 in Polish patients with bronchial asthmaMol Biol Rep3947494757201210.1007/s11033-011-1267-33294211PanekMPietrasTFabijanAZiołoJWieteskaLMałachowskaBFendlerWSzemrajJKunaPIdentification and association of the single nucleotide polymorphisms, C−509T, C+466T and T+869C, of the TGF-β1 gene in patients with asthma and their influence on the mRNA expression level of TGF-β1Int J Mol Med349759862014251191134152139ChomczynskiPSacchiNSingle-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extractionAnal Biochem162156159198710.1016/0003-2697(87)90021-22440339WinerJJungCKShackelIWilliamsPMDevelopment and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitroAnal Biochem2704149199910.1006/abio.1999.408510328763LivakKJSchmittgenTDAnalysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) methodMethods25402408200110.1006/meth.2001.1262DeRijkRSchaafMde KloetEGlucocorticoid receptor variants: Clinical implicationsJ Steroid Biochem Mol Biol81103122200210.1016/S0960-0760(02)00062-612137800SzabóVBorgulyaGFilkornTMajnikJBányászINagyZZThe variant N363S of glucocorticoid receptor in steroid-induced ocular hypertension in Hungarian patients treated with photorefractive keratectomyMol Vis136596662007175637202765477MajnikJPatócsABaloghKTóthMRáczKA rapid and simple method for detection of Asn363Ser polymorphism of the human glucocorticoid receptor geneJ Steroid Biochem Mol Biol92465468200410.1016/j.jsbmb.2004.08.005HuizengaNAKoperJWDe LangePPolsHAStolkRPBurgerHGrobbeeDEBrinkmannAODe JongFHLambertsSWA polymorphism in the glucocorticoid receptor gene may be associated with and increased sensitivity to glucocorticoids in vivoJ Clin Endocrinol Metab8314415119989435432
The effect of the NR3C1 gene (A) Tth111I, (B) ER22/23EK, (C) N363S, (D) BclI SNP genotypes and the TGF-β1 gene (E) C-509T SNP genotypes on the IL-5 mRNA expression levels in the control and patient groups. Data are presented as the median and the interquartile range. *P<0.05. NR3C1, nuclear receptor subfamily 3, group C, member 1; SNP, single nucleotide polymorphism; TGF-β1, transforming growth factor β1; IL, interleukin.
The effect of the NR3C1 gene (A) Tth111I, (B) ER22/23EK, (C) N363S, (D) BclI SNP genotypes and the TGF-β1 gene (E) C-509T SNP genotypes on the IL-15 mRNA expression level in the control and patient groups. Data are presented as the median and the interquartile range. *P<0.05. NR3C1, nuclear receptor subfamily 3, group C, member 1; SNP, single nucleotide polymorphism; TGF-β1, transforming growth factor β1; IL, interleukin.
Spirometric parameters of the analyzed groups.
Parameter
Controls
Patients
FEV1 (l)
Mean
2.99
2.15
Median
2.97
1.98
Min
1.40
0.37
Max
5.24
4.68
SD
0.82
0.90
FEV1 (%)
Mean
96.17
72.48
Median
95.00
73
Min
66.00
19
Max
128.00
125
SD
11.67
21.27
FVC (l)
Mean
3.77
3.15
Median
3.55
2.91
Min
1.87
1.11
Max
6.37
6.52
SD
1.04
1.08
FVC (%)
Mean
101.52
89.77
Median
101.00
89.00
Min
71.00
38.00
Max
150.00
134
SD
13.49
18.40
FEV1%FVC
Mean
79.49
67.19
Median
78.00
67.95
Min
64.15
33.33
Max
95.15
91.03
SD
6.28
12.32
FEV1 (L), forced expiratory volume in 1 sec, expressed in litres; Min, minimum; Max, maximum; SD, standard devation; FEV1%, percentage ratio of the measured to expected value, expressed as percentage of the expected value; FVC, forced vital capacity, expressed in litres; FVC%, percentage ratio of the measured to expected value, expressed as percentage of the expected value; FEV1%FVC, FEV1 to FVC ratio of forced vital capacity, expressed as absolute numbers.
Parameters of PCR reactions, used for genotyping of SNPs of the NR3C1 and TGF-β1 genes.