Molecular Medicine Reports Molecular Medicine Reports 1791-2997 1791-3004 D.A. Spandidos 10.3892/mmr.2016.6045 mmr-15-02-0813 Articles SIRT1-mediated regulation of oxidative stress induced by Pseudomonas aeruginosa lipopolysaccharides in human alveolar epithelial cells LiuXiaoli 1 YangTuo 2 SunTieying 3 ShaoKuiqing 4 Department of Respiratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, P.R. China Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15260, USA Department of Respiratory and Critical Care Medicine, Beijing Hospital Ministry of Health, Beijing 100730, P.R. China Department of Urinary Surgery, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing 100091, P.R. China Correspondence to: Dr Tieying Sun, Department of Respiratory and Critical Care Medicine, Beijing Hospital Ministry of Health, 1 Dahua Street, Dongcheng, Beijing 100730, P.R. China, E-mail: suntieying3@hotmail.com Dr Kuiqing Shao, Department of Urinary Surgery, Xiyuan Hospital of China Academy of Chinese Medical Sciences, 1 Xiyuancaochang, Hadian, Beijing 100091, P.R. China, E-mail: shaokuiqing@163.com 032017 14122016 15 2 813 818 10012016 19102016 Copyright © 2017, Spandidos Publications 2017

Sirtuin1 (SIRT1) is an NAD+-dependent deacetylase that exhibits multiple biological functions, including cell differentiation inhibition, transcription regulation, cell cycle regulation and anti-apoptosis. Lipopolysaccharides (LPS) are crucial virulence factors produced by Pseudomonas aeruginosa and serve an important role in adjusting the interactions between the host and the pathogen. However, the effect of SIRT1 in the regulation of LPS-induced A459 human alveolar epithelial cells (AECs) oxidative stress remains unclear. The cellular reactive oxygen species (ROS) production was examined in A549 cells that were supplemented with LPS. Relative cell signaling pathway proteins were further investigated by western blot analysis. It was identified that LPS downregulated SIRT1 expression, however, upregulated ROS generation, which was associated with the increase of nuclear factor (NF)-κB and acetyl-NF-κB. Activation of SIRT1 by resveratrol significantly reversed the effects of LPS on A549 cells. By contrast, inhibition of SIRT1 by nicotinamide had the opposite effects that enhance cell ROS production. Thus, the results indicated that SIRT1 serves an important role in the regulation of oxidative stress induced by LPS in human AECs.

 NF-κB acetyl-NF-κB ROS nicotinamide Introduction

Pseudomonas aeruginosa is one of the most prevalent opportunistic gram-negative bacteria that lead to pneumonia in immunocompromised patients through the production of numerous virulence factors, including enzymes and exotoxin, such as lipopolysaccharides (LPS) (1–5). P. aeruginosa accounts for 10–20% of all hospital-acquired infection (6), 18.1% of nosocomial pneumonia (7), 9.3% of ventilator-associated pneumonia (8) and 0.9–1.9% of community-acquired pneumonia requiring hospitalization (9–11). LPS are the most important pathogenic factors produced by P. aeruginosa and serve a major role in the interplay between the host and the pathogen. Type II alveolar epithelial cells (AECs) are major target cells of LPS locating in the alveolar corners, exhibiting various important functions including initiation of immune responses, fluid balance, mucus and surfactant production, and progenitor action to maintain the normal function of the alveoli (12–14).

Sirtuin1 (SIRT1), an NAD+-dependent class III protein deacetylase, belongs to the silent information regulator (Sir) family (15) and is suggested to serve diverse roles in gene silencing, stress resistance, apoptosis, senescence, aging and inflammation (16–19). Previous studies have demonstrated that SIRT1 can regulate cellular oxidative stress and its toxicity (20–23). Modulation of the acetylation of the p65 subunit of nuclear factor kB (NF-κB) in cells or tissues is the major means via which SIRT1 regulates cellular oxidative status (24).

At present, to the best of our knowledge, no reports have focused on the potential roles of SIRT1 in the regulation of oxidative stress induced by P. aeruginosa LPS in human AECs. In the current study, the effects of SIRT1 on the regulation of reactive oxidative stress, which is indicated by reactive oxygen species (ROS) generation, was examined in LPS-stimulated A549 cells.

 Materials and methods <sec> <title>Cell culture and drug treatment

A549 cells were maintained as previously described (25). Briefly cells were maintained in Dulbecco's modified Eagle's medium (DMEM) F-12 culture medium (GE Healthcare Life Sciences, Logan, UT, USA) containing 10% heat-inactivated fetal calf serum, 100 U/ml penicillin, and streptomycin respectively, in 25-cm2 culture flasks at 37°C in a humidified atmosphere with 5% CO2. LPS (Sigma-Aldrich; Merck Millipore, Darmstadt, Germany) was dissolved in sterilized phosphate-buffered saline (PBS). Cells were pretreated with nicotinamide (NAM) and resveratrol [Res; dissolved in dimethyl sulfoxide (DMSO); all from Sigma-Aldrich; Merck Millipore) for 1 h prior to LPS stimulation. The concentration of DMSO in the medium never exceeded 0.1% to avoid the toxicity of this solvent towards the A549 cells. Cells were divided into four groups, including a DMSO group (control), a Res group, a LPS treatment plus DMSO group (DMSO+LPS), and a LPS-treated Res group (Res+LPS).

 Fluorescence staining

A549 cells were cultured on six-well chamber slides and were washed with PBS three times for 5 min per wash, then subsequently incubated with ROS Fluorescent Probe-DHE (Vigorous Biotechnology Beijing Co., Ltd., Beijing, China) in serum-free DMEM F-12 medium for 30 min at 37°C in darkness and fixed in 4% paraformaldehyde for 30 min at room temperature. The slides were washed again and mounted. The slides were finally examined using a fluorescence microscope.

 Quantification of intracellular ROS

Intracellular ROS levels were quantified using ROS Fluorescent Probe-Dihydroethidium (DHE) to determine the oxidative stress towards the A549 cells in response to LPS stimulation. Following drug treatment, A549 cells were treated with dichlorofluorescin diacetate, a ROS-sensitive dye, and incubated for 30 min at 37°C in a humidified and dark atmosphere. A549 cells were harvested and suspended in PBS (0.14 M NaCl, 2.6 mM KCl, 8 mM Na2HPO4, and 1.5 mM KH2PO4). Relative fluorescence intensities in the A549 cells were analyzed with flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).

 Protein extraction and western blotting analysis

Following treatments, the cells were washed with ice-cold PBS three times. Proteins were extracted from the A549 cells in radioimmunoprecipitation assay buffer [1% Triton X-100, 150 mmol/l NaCl, 5 mmol/l EDTA and 10 mmol/l Tris-HCl (pH 7.0)] containing a protease inhibitor cocktail. Following sonication, cell lysates were subjected to centrifugation at 12,000 × g at 4°C for 15 min and the supernatants were collected as the total protein. Total protein (10–50 µg/lane) was electrophoresed and separated on a 10% SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride membrane (EMD Millipore, Billerica, MA, USA), which was then soaked in 8% non-fat milk in Tris-buffered saline with 1% Tween-20 (TBST; pH 7.6) at room temperature for 2 h to block non-specific binding sites. The membranes were then incubated at 4°C overnight with a rabbit SIRT1 polyclonal antibody (EMD Millipore; cat. no. 07-131) at a dilution of 1:3,000, a rabbit NF-κB polyclonal antibody (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; cat. no. sc-7178) at a dilution of 1:1,000 or a rabbit acetyl-NF-κB polyclonal antibody (Cell Signaling Technology, Inc., Danvers, MA, USA; cat. no. 3045) at a dilution of 1:1,000 on a rotating platform at 4°C. Subsequently, the membrane was rinsed in TBST (pH 7.6) 3 times and incubated with horseradish peroxidase-conjugated IgG antibodies diluted in TBST (1:5,000; Abmart, Inc., Shanghai, China; cat. no. M21003) for 2 h on a rotating platform at room temperature. Bands were visualized using a horseradish peroxidase developer, and background-subtracted signals were quantified on a laser densitometer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). A β-actin antibody (Santa Cruz Biotechnology, Inc.) was used to normalize the signal obtained for the total protein extracts. The protein bands were quantified using a PhosphorImager and ImageQuant (GE Healthcare Life Sciences) software analysis.

 Statistical analysis

Data are expressed as the mean ± standard error of the mean. Multiple comparisons were evaluated by one-way analysis of variance followed by Tukey's multiple-comparison test. P<0.05 was considered to indicate a statistically significant difference.

 Results <sec> <title>SIRT1 expression was reduced and ROS generation was elevated by LPS in A549 cells

In order to investigate the associations between ROS and SIRT1, the effects of LPS on SIRT1 protein expression and the generation of intracellular ROS following LPS treatment were assessed. A549 cells were treated with 10 µg/ml LPS for 12, 24 or 48 h or with 0.1, 1, or 10 µg/ml LPS for 48 h. Western blotting indicated that when the A549 cells were treated with 10 µg/ml LPS for 48 h, SIRT1 protein expression was reduced by 50% (Fig. 1A). LPS significantly reduced the level of SIRT1 expression in a dose-dependent manner. At doses of 1 and 10 µg/ml, the SIRT1 protein expression was downregulated to 28 and 51% compared with the control group, respectively (Fig. 1B). In addition, the generation of intracellular ROS was investigated. The results demonstrated that LPS elevated the generation of intracellular ROS in a time-dependent manner. At the 48 h time point, the generation of ROS was increased by approximately three times.

 SIR1 regulates LPS-induced A549 cell ROS generation associated with the activation of NF-κB pathway

In order to determine the level of oxidative stress induced by LPS, cells were treated with 10 µg/ml LPS for 48 h. Fluorescence staining illustrated that A549 cells incubated with LPS had increased ROS generation (Fig. 2A). Subsequently, the molecular mechanisms involved in A549 cell oxidative stress induced by LPS were investigated. Through 48-h LPS exposure, it was identified that LPS reduced SIRT1 protein expression and promoted NF-κB protein expression and NF-κB protein acetylation (Fig. 2B).

 A549 cell oxidative stress is attenuated by Res through the SIRT1-mediated deacetylation of NF-κB

Res, a natural polyphenol compound widely found in grapes, pine trees, peanuts and other plants and fruits, has comprehensive biochemical and physiological functions, including anti-cancer, anti-inflammatory and anti-oxidant activities, that regulate lipid actions (26–31). Res is widely accepted to activate SIRT1, thus, is frequently adopted as a SIRT1 enhancer. In order to determine the pivotal role of SIRT1 in regulating A549 cell oxidative stress induced by LPS, the cells were divided into four groups, including a DMSO group (control), a Res group, a LPS treatment plus DMSO group (DMSO+LPS), and a LPS-treated Res group (Res+LPS). The results suggested that Res induces SIRT1 protein expression in A549 cells in a dose-dependent manner. At doses of 5 and 20 µM, the increases in SIRT1 protein expression reached 58% and 91%, respectively (Fig. 3A). According to the results above, 20 µM was selected as the dose used in the subsequent experiments. It was demonstrated that ROS generation was reduced by Res in the A549 cells (Fig. 3C). The effects of Res on the activation of the SIRT1 pathways were partially reversed by LPS-stimulated downregulation of NF-κB deacetylation in the A549 cells (Fig. 3B).

NAM aggravates LPS-induced A549 cell oxidative stress by inhibiting the SIRT1 pathway. To further determine the role of SIRT1 in regulating the human alveolar epithelial A549 cell oxidative stress induced by LPS, NAM was selected as an inhibitor of SIRT1. The results illustrated that SIRT1 protein expression in the A549 cells was reduced by NAM in a dose-dependent manner. At doses of 5 and 10 mmol/ml, the reductions in SIRT1 protein expression were 27 and 51%, respectively (Fig. 4A). The ROS generation was examined in the A549 cells, indicating that the ROS generation in the A549 cells was increased (Fig. 4C). The effects of LPS on the inhibition of the SIRT1 pathway were aggravated by NAM in the A549 cells (Fig. 4B).

 Discussion

P. aeruginosa is a conditional Gram-negative bacterium leading to pneumonia in immunosuppressed patients and is colonized in the lower respiratory tract of patients with primary pulmonary diseases. In addition, ventilated patients are particularly susceptible to developing P. aeruginosa pneumonia (32,33). The mortality of ventilator-associated pneumonia due to P. aeruginosa has been observed to be significantly higher than that of other pathogens (34). LPS is one of predominant virulence factors produced by P. aeruginosa. The current study clarified the role of SIRT1 in regulating oxidative stress induced by P. aeruginosa LPS in human alveolar epithelial A549 cells.

Few studies have elucidated the association between SIRT1 and P. aeruginosa pneumonia-induced lung injury. The present study demonstrated SIRT1 expression had been significantly reduced in A549 cells that had been treated with LPS, in a dose-dependent manner. Following 48 h stimulation, the expression of SIRT1 was significantly reduced. Previous studies have reported that LPS increases the generation of reactive oxygen species in lungs, leading to lung damage (35,36). The results of the current study suggested that the generation of ROS was elevated in the A549 cells in a time-dependent manner following exposure to LPS, which is consistent with previous studies (37–40). Accordingly, it was hypothesized that the possible mechanisms of P. aeruginosa-induced lung damage may be associated with reactive oxidative stress in the alveolar type II epithelial cells induced by LPS.

SIRT1 is a NAM adenine dinucleotide (NAD+)-dependent histone deacetylase involved in multiple cellular functions (41,42). Previous studies have elucidated that SIRT1 serves an important role in oxidative stress (43–48). In addition, SIRT1 participates in adjusting the inflammatory response through modulation of the acetylation status of the p65 subunit of NF-κB, which is activated by oxidative stress (24). However, it is not currently clear whether SIRT1 is inhibited or activated under oxidative stress. In the current study, SIRT1 expression was reduced during the process of LPS-induced A549 cell oxidative stress, associated with upregulation of NF-κB and acetylation of NF-κB. This indicates that SIRT1 serves a crucial inhibitory role during the process of A549 cell oxidative stress induced by LPS through regulating the NF-κB signaling pathway. Therefore, reductions in SIRT1 expression induced by LPS may be associated with lung injury.

To further verify the pivotal role of SIRT1 in LPS-induced oxidative stress, a SIRT1 activator and inhibitor were used to result in functional gain and loss. Res has been previously reported as a widely used activator of SIRT1 (49,50). The current study demonstrates that A549 cell exposure to LPS increases SIRT1 expression. In addition, levels of intracellular ROS were reduced. The current study also hypothesized that the protective effect of Res against LPS-induced A549 cell oxidative stress may be associated with SIRT1 activation and modulation of deacetylation of NF-κB; and this was verified by western blotting analysis of SIRT1, NF-κB and acetylated NF-κB. To further verify the above hypothesis, NAM was utilized as a SIRT1 inhibitor to examine the role of SIRT1 regulation in LPS-induced A549 oxidative stress. The results demonstrated that treatment of A549 cells with NAM reduced SIRT1 protein expression in the A549 cells in a dose-dependent manner. The A549 cell oxidative stress induced by LPS was aggravated by NAM, and ROS generation was increased in the A549 cells. The effects of LPS on SIRT1 pathway inhibition were aggravated by NAM in the A549 cells. Taken together, these results indicate the central and pivotal role of SIRT1 in LPS-induced oxidative stress in A549 cells via modulation of the NF-κB pathway, which maybe a potential translational target for treating P. aeruginosa pneumonia.

In conclusion, the results of the current study suggest that A549 cell oxidative stress is induced by LPS. SIRT1 serves a central role in the oxidative stress induced by LPS through inducing NF-κB deacetylation. Res defends against LPS-induced A549 cell oxidative stress via activation of SIRT1 and NAM aggravates the A549 cell oxidative stress induced by LPS. SIRT1 activators are suggested as a promising therapeutic interventional target for P. aeruginosa infections.

 Acknowledgements

The present study was supported by the National Natural Science Foundation of China (grant no. 81270495).

 References QuartinAAScerpellaEGPuttaguntaSKettDHA comparison of microbiology and demographics among patients with healthcare-associated, hospital-acquired and ventilator-associated pneumonia: A retrospective analysis of 1184 patients from a large, international studyBMC Infect Dis13561201310.1186/1471-2334-13-561242797014222644 RestrepoMIAnzuetoAThe role of gram-negative bacteria in healthcare-associated pneumoniaSemin Respir Crit Care Med306166200910.1055/s-0028-111981019199188 ZilberbergMDShorrAFEpidemiology of healthcare-associated pneumonia (HCAP)Semin Respir Crit Care Med301015200910.1055/s-0028-111980419199182 Di PasqualeMFerrerMEsperattiMCrisafulliEGiuntaVLi BassiGRinaudoMBlasiFNiedermanMTorresAAssessment of severity of ICU-acquired pneumonia and association with etiologyCrit Care Med42303312201410.1097/CCM.0b013e3182a272a223989176 SadikotRTBlackwellTSChristmanJWPrinceASPathogen-host interactions in Pseudomonas aeruginosa pneumoniaAm J Respir Crit Care Med17112091223200510.1164/rccm.200408-1044SO156954912718459 RamosGPRochaJLTuonFFSeasonal humidity may influence Pseudomonas aeruginosa hospital-acquired infection ratesInt J Infect Dis17e757e761201310.1016/j.ijid.2013.03.00223639485 GaynesREdwardsJRNational Nosocomial Infections Surveillance System: Overview of nosocomial infections caused by gram-negative bacilliClin Infect Dis41848854200510.1086/43280316107985 RelloJOllendorfDAOsterGVera-LlonchMBellmLRedmanRKollefMHVAP Outcomes Scientific Advisory Group: Epidemiology and outcomes of ventilator-associated pneumonia in a large US databaseChest12221152121200210.1378/chest.122.6.211512475855 FangGDFineMOrloffJArisumiDYuVLKapoorWGraystonJTWangSPKohlerRMuderRRNew and emerging etiologies for community-acquired pneumonia with implications for therapy. A prospective multicenter study of 359 casesMedicine (Baltimore)69307316199010.1097/00005792-199009000-000042205784 BlanquerJBlanquerRBorrásRNauffalDMoralesPMenéndezRSubíasIHerreroLRedónJPascualJAetiology of community acquired pneumonia in Valencia, Spain: A multicentre prospective studyThorax46508511199110.1136/thx.46.7.5081908605463249 NeillAMMartinIRWeirRAndersonRChereshskyAEptonMJJacksonRSchousboeMFramptonCHuttonSCommunity acquired pneumonia: Aetiology and usefulness of severity criteria on admissionThorax5110101016199610.1136/thx.51.10.10108977602472650 CrapoJDBarryBEGehrPBachofenMWeibelERCell number and cell characteristics of the normal human lungAm Rev Respir Dis12633233719827103258 HerzogELBrodyARColbyTVMasonRWilliamsMCKnowns and unknowns of the alveolusProc Am Thorac Soc5778782200810.1513/pats.200803-028HR187573172645265 PerlmanCEBhattacharyaJAlveolar expansion imaged by optical sectioning microscopyJ Appl Physiol (1985)10310371044200710.1152/japplphysiol.00160.200717585045 ImaiSArmstrongCMKaeberleinMGuarenteLTranscriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylaseNature403795800200010.1038/3500162210693811 ChungSYaoHCaitoSHwangJWArunachalamGRahmanIRegulation of SIRT1 in cellular functions: Role of polyphenolsArch Biochem Biophys5017990201010.1016/j.abb.2010.05.003204508792930135 RahmanIKinnulaVLGorbunovaVYaoHSIRT1 as a therapeutic target in inflammaging of the pulmonary diseasePrev Med54SupplS20S28201210.1016/j.ypmed.2011.11.01422178470 YaoHRahmanIPerspectives on translational and therapeutic aspects of SIRT1 in inflammaging and senescenceBiochem Pharmacol8413321339201210.1016/j.bcp.2012.06.031227965663482299 YaoHChungSHwangJWRajendrasozhanSSundarIKDeanDAMcBurneyMWGuarenteLGuWRöntyMSIRT1 protects against emphysema via FOXO3-mediated reduction of premature senescence in miceJ Clin Invest12220322045201210.1172/JCI60132225468583366403 DeyNChattopadhyayDJChatterjeeIBMolecular mechanisms of cigarette smoke-induced proliferation of lung cells and prevention by vitamin CJ Oncol2011561862201110.1155/2011/561862217728443136156 KumeSHanedaMKanasakiKSugimotoTArakiSIsonoMIsshikiKUzuTKashiwagiAKoyaDSilent information regulator 2 (SIRT1) attenuates oxidative stress-induced mesangial cell apoptosis via p53 deacetylationFree Radic Biol Med4021752182200610.1016/j.freeradbiomed.2006.02.01416785031 ChuaKFMostoslavskyRLombardDBPangWWSaitoSFrancoSKaushalDChengHLFischerMRStokesNMammalian SIRT1 limits replicative life span in response to chronic genotoxic stressCell Metab26776200510.1016/j.cmet.2005.06.00716054100 LiYXuWMcBurneyMWLongoVDSirT1 inhibition reduces IGF-I/IRS-2/Ras/ERK1/2 signaling and protects neuronsCell Metab83848200810.1016/j.cmet.2008.05.004185906912822839 HahYSCheonYHLimHSChoHYParkBHKaSOLeeYRJeongDWKimHOHanMKLeeSIMyeloid deletion of SIRT1 aggravates serum transfer arthritis in mice via nuclear factor-κB activationPLoS One9e87733201410.1371/journal.pone.0087733244983643912001 YangTChenMSunTSimvastatin attenuates TGF-β1-induced epithelial-mesenchymal transition in human alveolar epithelial cellsCell Physiol Biochem31863874201310.1159/00035010423817018 HsiehTCWuSTBennettDJDoonanBBWuEWuJMFunctional/activity network (FAN) analysis of gene-phenotype connectivity liaised by grape polyphenol resveratrolOncotarget738670386802016272329435122419 Blanquer-RossellóMDHernández-LópezRRocaPOliverJValleAResveratrol induces mitochondrial respiration and apoptosis in SW620 colon cancer cellsBiochim Biophys ActaOct172016(Epub ahead of print) CaddeoCNacherAVassalloAArmentanoMFPonsRFernàndez-BusquetsXCarboneCValentiDFaddaAMManconiMEffect of quercetin and resveratrol co-incorporated in liposomes against inflammatory/oxidative response associated with skin cancerInt J Pharm513153163201610.1016/j.ijpharm.2016.09.01427609664 Riveiro-NaveiraRRValcárcel-AresMNAlmonte-BecerrilMVaamonde-GarcíaCLoureiroJHermida-CarballoLLópez-PeláezEBlancoFJLópez-ArmadaMJResveratrol lowers synovial hyperplasia, inflammatory markers and oxidative damage in an acute antigen-induced arthritis modelRheumatology (Oxford)5518899000201610.1093/rheumatology/kew25527354682 JeongHSamdaniKJYooDHLeeDWKimNHYooISLeeJHResveratrol cross-linked chitosan loaded with phospholipid for controlled release and antioxidant activityInt J Biol Macromol93757766201610.1016/j.ijbiomac.2016.09.01827614196 TangLYangFFangZHuCResveratrol Ameliorates Alcoholic Fatty Liver by Inducing AutophagyAm J Chin Med4412071220201610.1142/S0192415X1650067127627919 ArabiYAl-ShirawiNMemishZAnzuetoAVentilator-associated pneumonia in adults in developing countries: A systematic reviewInt J Infect Dis12505512200810.1016/j.ijid.2008.02.01018502674 LeeMSWalkerVChenLFSextonDJAndersonDJThe epidemiology of ventilator-associated pneumonia in a network of community hospitals: A prospective multicenter studyInfect Control Hosp Epidemiol34657662201310.1086/670991237390683977705 TumbarelloMDe PascaleGTrecarichiEMSpanuTAntonicelliFMavigliaRPennisiMABelloGAntonelliMClinical outcomes of Pseudomonas aeruginosa pneumonia in intensive care unit patientsIntensive Care Med39682692201310.1007/s00134-013-2828-923370828 ChianCFChiangCHYuan-JungCChuangCHLiuSLYi-HanJZhangHRyuJHApocynin attenuates lipopolysaccharide-induced lung injury in an isolated and perfused rat lung modelShock38196202201210.1097/SHK.0b013e31825a198222592748 BootsAWGerloffKBartholoméRvan BerloDLedermannKHaenenGRBastAvan SchootenFJAlbrechtCSchinsRPNeutrophils augment LPS-mediated pro-inflammatory signaling in human lung epithelial cellsBiochim Biophys Acta182311511162201210.1016/j.bbamcr.2012.04.01222575681 ChoRLYangCCLeeITLinCCChiPLHsiaoLDYangCMLipopolysaccharide induces ICAM-1 expression via a c-Src/NADPH oxidase/ROS-dependent NF-κB pathway in human pulmonary alveolar epithelial cellsAm J Physiol Lung Cell Mol Physiol310L639L657201610.1152/ajplung.00109.201426747779 KinoshitaMSanukiTYamadaYSasakiAYoshieTKurodaDKanzawaMItouTA case of esophageal mucosa-associated lymphoid tissue lymphoma diagnosed using endoscopic ultrasound-guided fine-needle aspirationNihon Shokakibyo Gakkai Zasshi1136370201626743555 YangLLiDZhuoYZhangSWangXGaoHProtective Role of Liriodendrin in Sepsis-Induced Acute Lung InjuryInflammation3918051813201610.1007/s10753-015-0231-027498121 LiuXPeiCYanSLiuGLiuGChenWCuiYLiuYNADPH oxidase 1-dependent ROS is crucial for TLR4 signaling to promote tumor metastasis of non-small cell lung cancerTumour Biol3614931502201510.1007/s13277-014-2639-925592377 YamamotoHSchoonjansKAuwerxJSirtuin functions in health and diseaseMol Endocrinol2117451755200710.1210/me.2007-007917456799 KongXXWangRLiuXJZhuLLShaoDChangYSFangFDFunction of SIRT1 in physiologyBiochemistry (Mosc)74703708200910.1134/S000629790907001319747089 HwangJWSundarIKYaoHSellixMTRahmanICircadian clock function is disrupted by environmental tobacco/cigarette smoke, leading to lung inflammation and injury via a SIRT1-BMAL1 pathwayFASEB J28176194201410.1096/fj.13-232629240257283868829 GuoHChenYLiaoLWuWResveratrol protects HUVECs from oxidized-LDL induced oxidative damage by autophagy upregulation via the AMPK/SIRT1 pathwayCardiovasc Drugs Ther27189198201310.1007/s10557-013-6442-423358928 HuYXCuiHFanLPanXJWuJHShiSZCuiSYWeiZMLiuLResveratrol attenuates left ventricular remodeling in old rats with COPD induced by cigarette smoke exposure and LPS instillationCan J Physiol Pharmacol9110441054201310.1139/cjpp-2012-046424289075 YangYDuanWLinYYiWLiangZYanJWangNDengCZhangSLiYSIRT1 activation by curcumin pretreatment attenuates mitochondrial oxidative damage induced by myocardial ischemia reperfusion injuryFree Radic Biol Med65667679201310.1016/j.freeradbiomed.2013.07.00723880291 LimHDKimYSKoSHYoonIJChoSGChunYHChoiBJKimECCytoprotective and anti-inflammatory effects of melatonin in hydrogen peroxide-stimulated CHON-001 human chondrocyte cell line and rabbit model of osteoarthritis via the SIRT1 pathwayJ Pineal Res53225237201210.1111/j.1600-079X.2012.00991.x22507555 BrandlAMeyerMBechmannVNerlichMAngelePOxidative stress induces senescence in human mesenchymal stem cellsExp Cell Res31715411547201110.1016/j.yexcr.2011.02.01521376036 HowitzKTBittermanKJCohenHYLammingDWLavuSWoodJGZipkinREChungPKisielewskiAZhangLLSmall molecule activators of sirtuins extend Saccharomyces cerevisiae lifespanNature425191196200310.1038/nature0196012939617 WoodJGRoginaBLavuSHowitzKHelfandSLTatarMSinclairDSirtuin activators mimic caloric restriction and delay ageing in metazoansNature430686689200410.1038/nature0278915254550

SIRT1 protein expression was reduced by LPS in the A549 cells. A549 cells were exposed to 10 µg/ml LPS for (A) 6, 12, 24 or 48 h or (B) 0.1, 1 or 10 µg/ml LPS for 48 h. Western blotting illustrated that SIRT1 protein expression in the A549 cells was reduced significantly at 48 h (A), and was reduced by LPS in the A549 cells in a dose-dependent manner (B). (C) A549 cells were treated with 10 µg/ml LPS for 6, 12, 24 and 48 h. Levels of intracellular ROS in the A549 cells were quantified using flow cytometry. ROS generation was increased by LPS in the A549 cells in a time-dependent manner. Data are presented as the mean ± standard error, n=3 independent experiments. *P<0.05, **P<0.01 vs. control. SIRT1, sirtuin 1; LPS, lipopolysaccharides.

SIRT1 and NF-κB regulate the A549 cell oxidative stress induced by LPS. (A) A549 cells were treated with 10 µg/ml LPS for 48 h and were stained with ROS Fluorescent Probe-Dihydroethidium to indicate levels of ROS (magnification, ×200). (B) Western blotting illustrated that LPS reduces SIRT1 protein expression and stimulates the level of acetylated NF-κB. Data are presented as the mean ± standard error, n=3 independent experiments. **P<0.01 vs. control. SIRT1, sirtuin 1; NF-κB, nuclear factor κB; ROS, reactive oxygen species; Con, control; LPS, lipopolysaccharides; Ace-NF-κB, acetyl- NF-κB.

A549 cell oxidative stress is attenuated by Res through the SIRT1-mediated deacetylation of NF-κB. A549 cells were exposed to 0.1, 1, 5 and 20 µM Res for 48 h. (A) Western blotting illustrated that SIRT1 protein expression was increased by Res in the A549 cells in a dose-dependent manner. (B) The effects of Res on the activation of the SIRT1 pathways were partially reversed by LPS via NF-κB deacetylation in the A549 cells. (C) Res reduces ROS generation in A549 cells. Data are presented as the mean ± standard error, n=3 independent experiments. *P<0.05, **P<0.01 vs. control. Res, resveratrol; SIRT1, sirtuin 1; NF-κB, nuclear factor κB; LPS, lipopolysaccharides; DMSO, dimethyl sulfoxide; Ace-NF-κB, acetyl- NF-κB.

NAM aggravates LPS-induced A549 cell oxidative stress by inhibiting the SIRT1 pathway. A549 cells were exposed to 2.5, 5 or 10 mM NAM for 48 h. (A) Western blotting illustrated that SIRT1 protein expression was reduced in the A549 cells in a dose-dependent manner. (B) The effect of LPS on the inhibition of the SIRT1 pathways was aggravated by NAM in the A549 cells. (C) NAM increases ROS generation in A549 cells. Data are presented as the mean ± standard error, n=3 independent experiments. *P<0.05, **P<0.01 vs. control. NAM, nicotinamide; LPS, lipopolysaccharides; SIRT1, sirtuin 1; ROS, reactive oxygen species; Con, control; DMSO, dimethyl sulfoxide.

Lipopolysaccharides can increase ROS generation in A549 cells. SIRT1 serves an important role in regulating human alveolar epithelial A549 cell oxidative stress induced by lipopolysaccharides through NF-κB deacetylation. ROS, reactive oxygen species; SIRT1, sirtuin 1; NF-κB, nuclear factor κB.