Introduction

Molecular Medicine Reports

1791-2997 1791-3004

D.A. Spandidos

10.3892/mmr.2019.10430

mmr-20-02-1966

Articles

Differential expression profile analysis of cisplatin-regulated miRNAs in a human gastric cancer cell line

Yin

Chunlin

1 Zheng

Xianxian

2 Xiang

Heping

3 Li

3 Gao

Ming

3 Meng

Xiangling

1 Yang

Kai

1Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China 2Department of Clinical Laboratory, Hefei Binhu Hospital, Hefei, Anhui 230601, P.R. China 3Department of Emergency Surgery, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China 4Department of Clinical Laboratory, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China

Correspondence to: Dr Xiangling Meng, Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, Anhui 230022, P.R. China, E-mail: xianglingmeng55@sina.cn

082019

26062019

20 2 1966 1976 14082018 22052019

2019

Cisplatin, one of the most commonly used drugs in combination chemotherapy, is an effective anti-tumor agent widely used for diverse tumor types. MicroRNAs (miRNAs/miRs) are involved in the occurrence, development, diagnosis and treatment of cancer. Therefore, the aim of the current study was to explore whether cisplatin exerts anticancer effects by causing differential expression of miRNAs in human gastric cancer cells. The human gastric cancer cell line NCI-N87 was cultured with a certain dose of cisplatin and high-throughput sequencing combined with reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to detect cisplatin-regulated miRNAs. miRNAs upregulated and downregulated following cisplatin exposure were analyzed. High-throughput sequencing revealed 33 upregulated and 16 downregulated miRNAs. A total of five significantly upregulated and five significantly downregulated miRNAs were identified by RT-qPCR. The expression levels of hsa-miR-1246 and hsa-miR-892b were consistent with the results obtained from high-throughput sequencing. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway clustering of cisplatin-regulated miRNAs revealed that the miRNAs regulated genes involved in several biological processes and signaling pathways. The results obtained in the current study suggested that cisplatin may exert an important anticancer effect in gastric cancer via complex biological processes and signaling pathways.

cisplatin gastric cancer microRNA high-throughput sequencing differential expression

Introduction

Cisplatin (cis-dichlorodiammine platinum) is a platinum-based chemotherapeutic agent used for the treatment of several types of cancer. It binds to DNA and inhibits DNA replication, resulting in apoptosis (1,2). Cisplatin has a strong anti-cancer effect and synergistic action with various anti-tumor drugs and is therefore one of the most commonly used drugs in combination chemotherapy (3,4). It is primarily used in the treatment of ovarian, prostate, testicular, lung and gastric cancer (3,4). Cisplatin is associated with dose-dependent side effects (5,6), including gastrointestinal reactions (7), bone marrow suppression (8), liver and kidney damage (9), ototoxicity (10) and neurotoxicity (11). Renal toxicity is frequently encountered, with a clinical incidence rate of 25–35% (12–14). The side effects associated with cisplatin have limited its use and clinical efficacy (15,16). Therefore, strategies to reduce these side effects are required in the field of antitumor therapy.

Gastric cancer, which is a malignant tumor originating from the gastric mucosal epithelium (17), is the fourth most common cancer and the second most common cause of cancer-associated mortality worldwide (18). According to global cancer statistics in 2012, gastric cancer accounted for approximately one million new cases and over 700,000 mortalities, representing 8% of all cancer cases and 9.7% of all cancer mortalities, and almost half of these patients came from China (19). Although the morbidity and mortality of gastric cancer has decreased in recent years, the 5-year survival rate remains low (<30%) (20,21). Radiotherapy and chemotherapy combined with surgical resection is the main treatment for gastric cancer, but the recurrence rate is very high (20,21). Cisplatin is currently a first line chemotherapeutic agent for gastric cancer (22,23), particularly for patients with advanced gastric cancer.

Cisplatin has a better curative effect for gastric cancer. The target microRNAs (miRNAs) of cisplatin remain unknown. In the current study, the human gastric cancer cell line NCI-N87 was used to identify differentially expressed miRNAs following exposure to cisplatin. High-throughput sequencing and bioinformatics analysis identified 49 differentially expressed miRNAs, including 33 upregulated miRNAs and 16 downregulated miRNAs following treatment with cisplatin. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) results revealed that the expression level of hsa-miR-1246 and hsa-miR-892b were consistent with the microarray data. The results obtained in the current study suggested that cisplatin may serve a role in the treatment of gastric cancer by regulating the expression of the aforementioned miRNAs, which may allow the development of novel therapeutic agents for gastric cancer.

Materials and methods <sec> <title>Cell culture and cisplatin stimulation

The human gastric cancer cell line NCI-N87 (American Type Culture Collection) was cultured in Dulbecco's Modified Eagles Medium (DMEM; HyClone; GE Healthcare Life Sciences) supplemented with 10% fetal bovine serum (HyClone; GE Healthcare Life Sciences) and 1% penicillin and streptomycin (Suzhou Zeke Biotech, Co., Ltd.). Cells were maintained in an incubator at 5% CO₂ and 37°C. When the cell confluence reached 70–80%, the cells were treated 0, 7.5, 15, 30 and 60 µg/ml cisplatin for 48 h at 37°C. Cell viability was subsequently assessed using an MTT assay and applied for further investigation.

MTT assay

A total of 1×10⁴ NCI-N87 cells were seeded in 96-well plates with complete DMEM and incubated at 37°C for 48 h. Cells were treated with MTT (Changchun Keygen Biological Products Co., Ltd.) according to the manufacturer's instructions and incubated in an incubator at 5% CO₂ and 37°C for 4 h. The purple formazan crystals were subsequently dissolved in 150 µl dimethyl sulfoxide (Sigma-Aldrich; Merck KGaA) at room temperature for 10 min, and analyzed at a wavelength of 490 nm using a microplate reader.

High-throughput sequencing

The total RNA of NCI-N87 cells treated with and without cisplatin was extracted using the Takara MiniBEST Universal RNA Extraction Kit (cat. no. 9767; Takara Bio, Inc.) according to the manufacturer's protocol and sent to Shanghai Personal Biotechnology Co., Ltd. for high-throughput sequencing using the Illumina NextSeq500 platform (Illumina, Inc.).

RT-qPCR assay

The total RNA was extracted using the Takara MiniBEST Universal RNA Extraction Kit (cat. no. 9767; Takara Bio, Inc.). Total RNA was reverse transcribed and qPCR was performed using a PrimeScript One Step RT-PCR Kit Ver.2 (cat. no. RR057A; Takara Bio, Inc.) according to the manufacturers' instructions. The reaction mixture, which consisted of 2 µl 5X PrimeScript RT Master mix (included in the PrimeScript One Step RT-PCR Kit), 500 ng total RNA, RNase-free dH₂O up to 10 µl, was prepared and reacted at 37°C for 15 min, followed by 85°C for 5 sec and 4°C indefinitely. qPCR was subsequently performed using the following mixture: 10 µl of 2× SYBR Premix Ex Taq II (included in the PrimeScript One Step RT-PCR Kit), 0.8 µl of forward primer, 0.8 µl of reverse primer, 0.4 µl of ROX Reference Dye II (included in the kit), 2 µl of cDNA and 6 µl of ddH₂O. U6 was used as reference gene. The thermocycling conditions were as follows: One cycle of 95°C for 30 sec, 40 cycles of 95°C for 5 sec, 60°C for 34 sec and 4°C indefinitely. The data was analyzed using SDS software (version 1.4; Applied Biosystems; Thermo Fisher Scientific, Inc.) based on the 2^−ΔΔCq method (24,25), and histogram analysis was performed using Origin software (version 10.5.30; http://www.originlab.com/index.aspx?go=PRODUCTS/Origin). All primers are listed in Table I.

Bioinformatics analysis of differentially expressed miRNAs in NCI-N87 cells following cisplatin treatment

High-throughput sequencing was performed with >3 biological replicates in each group, and t-tests were used to select differentially expressed genes using fold change (FC). FC differences in the cisplatin-treated (3 replicates) and control (3 replicates) groups were investigated. P<0.05 and log2 (FC)>2 (upregulated) or log2(FC)<-2 (downregulated) were used to identify differentially expressed miRNAs. The differentially expressed miRNAs were used to construct heatmaps and perform volcano analysis using R (version 3.5, www.R-project.org). The distribution frequency of the miRNAs following cisplatin treatment was determined by the adjusted P-value (Padj) according to the analysis of differentially expressed miRNAs (26).

Gene Ontology (GO) clustering

The GO database (geneontology.org) contains three aspects of functional information: The biological process (BP), the cellular component (CC), and the molecular function (MF) (27,28). These functions were organized into the ‘Directed Acyclic Graph’ (DAG) structure according to the size of the concept. Genes are considered significantly enriched based on the ratio of the observed GO term for all genes/GO term for a single gene set. In the current study, each gene annotated to a GO term was extensively annotated to all parent nodes of that node. Each GO term-enriched P-value was calculated using the hyper-geometric distribution method, and the P-value was corrected using the false discovery rate. P<0.05 was considered to indicate a statistically significant difference. Redundant GO terms within the threshold were removed; GO terms terminal to the DAG graph which were significantly enriched were selected.

Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway clustering

The KEGG database (https://www.genome.jp/kegg/) is the world's largest database for analyzing gene function and genomic information from a biological pathway perspective system (29). It contains metabolites, enzymes, biochemical reactions, gene regulation and protein interactions. In the current study, the hypergeometric distribution method was used to study biological pathways. The F-value was used to correct the P-value and the threshold was set to 0.05. Finally, a KEGG signaling pathway in which differentially expressed genes were significantly enriched was determined. The network was analyzed using the Cytoscape software (version 3.7.1; http://cytoscape.org/download.html).

Target prediction

miR-1246-regulated genes were obtained using TargetScan (version 7.2; http://www.targetscan.org/vert_72/) (30) and the miRDB database (http://www.mirdb.org/) (31).

Statistical analysis

All data were expressed as mean ± standard deviation. One-way ANOVA followed by the Least Significant Difference test was performed. The Student's t-test was used to analyze two groups. Statistical analysis was performed using SPSS software (version 22.0; IBM Corp.). P<0.05 and P<0.01 were considered to indicate significant and highly significant statistical differences, respectively.

Results <sec> <title>Differential expression profile analysis of cisplatin-regulated miRNAs

According to the quantile normalization exhibited in Fig. 1, the length of the miRNAs identified was 19–25 nucleotides. Bioinformatics analysis revealed a total of 33 upregulated miRNAs and 16 downregulated miRNAs in NCI-N87 cells following cisplatin treatment (Table II). A heatmap was generated based on these differentially expressed miRNAs, where red represented the upregulated miRNAs and green represented the downregulated miRNAs (Fig. 2A). The distribution frequency of the miRNAs following cisplatin treatment was determined by the Padj according to the analysis of differentially expressed miRNAs. The volcano plot presented in Fig. 2B reflected the similarities and the differences in gene expression among the miRNAs regulated by cisplatin treatment.

Expression of hsa-miR-1246 is significantly increased and that expression of hsa-miR-892b is significantly decreased following cisplatin treatment

To further validate the differently expressed miRNAs identified in the microarray, the top five significantly upregulated miRNAs, including hsa-miR-3609, hsa-miR-4497, hsa-miR-1246, hsa-miR-4301 and hsa-miR-6724-5p, and the top five significantly downregulated miRNAs, including hsa-miR-4508, hsa-miR-33b-3p, hsa-miR-1268b, hsa-miR-153-5p and hsa-miR-892b, were selected for RT-qPCR analysis. The expression level of hsa-miR-1246 was significantly increased, and that of hsa-miR-892b was significantly decreased, following treatment of cisplatin compared with the control (Fig. 3), consistent with the results obtained from the microarray. Therefore, hsa-miR-1246 and hsa-miR-892b were selected for subsequent analysis.

GO clustering of hsa-miR-1246 and hsa-miR-892b-regulated genes

For GO analysis, hsa-miR-1246-regulated genes were obtained using TargetScan and the miRDB database. A total of 14 intersecting genes were obtained as presented in the Venn diagram in Fig. 4A. GO clustering was performed to investigate MF, BF and CC associated with the miRs. As presented in Fig. 4B, 10 BPs were identified, including ‘transporter activity’, ‘substrate-specific transporter activity’, ‘regulation of cellular process’, ‘regulation of BP’, ‘protein binding’, ‘primary metabolic process’, ‘organic substance metabolic process’, ‘organic cyclic compound binding’, ‘organelle’ and ‘nitrogen compound metabolic process’. Similarly, for hsa-miR-892b regulated genes, a total of 9 intersecting genes was obtained as presented in Venn diagram in Fig. 4C. The GO clustering revealed six processes, including ‘regulation of cellular process’, ‘regulation of biological process’, ‘protein binding’, ‘primary metabolic process’, ‘plasma membrane’ and ‘organic substance metabolic process’ (Fig. 4D). The results obtained indicated that cisplatin-regulated miRNAs participate in a variety of BPs and may have important regulatory roles in the tumorigenesis of gastric cancer.

KEGG pathway clustering of hsa-miR-1246 and hsa-miR-892b regulated genes

As presented in Fig. 5A, the KEGG pathway enrichment based on hsa-miR-1246 regulated genes revealed three pathways, including ‘oxidative stress response’, ‘axon guidance mediated by netrin’ and ‘salvage pyrimidine deoxyribonucleotides’. Cytoscape software was used to further analyze the hsa-miR-1246 regulated genes (Fig. 5B).

In addition, as presented in Fig. 5C, the KEGG pathway clustering based on the hsa-miR-892b regulated genes were involved in 11 pathways, including ‘Wnt signaling pathway’, ‘cadherin signaling pathway’ and ‘notch signaling pathway’. Cytoscape software was used to further analyze the hsa-miR-892b regulated genes (Fig. 5D).

Discussion

In the current study, the human gastric cancer cell line NCI-N87 was cultured with the 15 µg/ml of cisplatin, and high-throughput sequencing combined with RT-qPCR was used to detect cisplatin-regulated genes. Finally, miRNAs that were positively and negatively associated with cisplatin treatment were analyzed statistically, and confirmed 33 positively- and 16 negatively-associated miRNAs. The top five significantly upregulated and top five significant down-regulation miRNAs were selected to further verify the results obtained from the microarray analysis. It was revealed that the expression levels of hsa-miR-1246 and hsa-miR-892b were consistent with microarray analysis. GO and KEGG pathway clustering of cisplatin-regulated miRNAs was performed, and it was demonstrated that the two miRNAs were involved in several BPs and signaling pathways. Therefore, cisplatin may exert an important anticancer effect in gastric cancer by affecting biological processes and signal pathways; however further investigation is required.

Gastric cancer is the second leading cause of cancer-associated mortalities in the world, and has a poor prognosis as the majority of patients are diagnosed at an advanced stage (18). Although there have been improvements in the early diagnosis and treatment of gastric cancer, the prognosis of patients with gastric cancer is generally not favorable (32,33). One of the main reasons for this observation is primary or secondary drug resistance during chemotherapy (34). A number of mechanisms have been proposed to explain the phenomenon of drug resistance in cancer cells (34). Previous studies revealed that tumor drug-resistance mechanisms included oncogene activation, anti-oncogene inactivation, reduced intracellular drug concentration, drug target molecular changes, metabolism detoxification, enhanced DNA damage repair function and inhibition of tumor cell apoptosis (28–30). Therefore, elucidating the mechanisms underlying the occurrence and development of gastric cancer drug resistance in order to reverse this process may improve treatment outcomes in gastric cancer. Cisplatin is one of the most commonly used drugs in the treatment of advanced cancer, including gastric cancer (35). Despite the high sensitivity of patients with gastric cancer to cisplatin at initial administration, a substantial number of patients develop drug resistance, which is one of the major causes of treatment failure (36). Cisplatin-based combination chemotherapy is the most effective treatment for metastatic gastric cancer (37). However, chemoresistance remains an obstacle for the effective treatment of the disease (38).

miRNAs are small non-coding RNAs that function as endogenous silencers of various target genes (39). Mature miRNAs bind to the 3′untranslated regions of target mRNA, leading to the silencing of mRNA (39). miRNAs regulate biological functions, including cell proliferation, differentiation and apoptosis. A number of studies have demonstrated that miRNAs are involved in the occurrence, development, diagnosis and treatment of cancer (40,41). Certain miRNAs, such as miR-34a, are downregulated in various types of cancer and act as tumor suppressors (42,43). Conversely, miRNAs such as miR-155 are reportedly overexpressed in various types of cancer, and act as oncogenes (44). Changes in miRNA expression contribute to the initiation and progression of cancer (45,46). The association between miRNAs and tumors suggests that miRNAs may be altered in patients with gastric cancer (47,48). A previous study demonstrated that miR-218 increased chemosensitivity to cisplatin in vitro and in vivo by inducing apoptosis (18). The current study revealed that cisplatin upregulated hsa-miR-1246 and downregulated hsa-miR-892b. Few studies have investigated the role of hsa-miR-1246 in cancer; however, it has been reported that miR-1246 is associated with the apoptosis and migration of cancer cells (49). Furthermore, miR-1246 has a p53-responsive element in its promoter region; p53 was determined to induced hsa-miR-1246 expression (49). In non-small cell lung cancer, hsa-miR-1246 plays an important role in the invasion of cancer cells by promoting proliferation, and sphere and colony formation (50); however, its role in gastric cancer has not been reported. The role of hsa-miR-892b in tumor progression, particularly in gastric cancer, remains unclear.

The results obtained in the current study suggested that hsa-miR-1246 and hsa-miR-892b may play important roles in the progression of gastric cancer via certain BPs and signaling pathways; however, future studies are required to confirm this. The high incidence of false positives obtained in the current study may be associated with the small sample size and the use of only one cell line. A larger sample size and other cell lines are required for future work.

The current study investigated cisplatin-regulated genes in NCI-N87 cells. The results obtained may provide a theoretical basis for a novel approach to further study the association between miRNA and cisplatin resistance in gastric cancers, as well as its possible mechanism. Importantly, differential miRNA expression patterns may provide a solid basis for further functional studies to identify potential oncogenic or tumor suppressor miRNAs in gastric cancer during cisplatin resistance. Of note, as our study was conducted in a preliminary matter, further investigation is required to determine the direct target genes of cisplatin and the mechanism underlying the effects of cisplatin on gastric cancer.

Acknowledgements

Not applicable.

Funding

No funding was received.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

CY and XM conceived and designed the experiments. CY, XZ, HX and HL performed the experiments. CY, XZ, MG and KY contributed to the acquisition and analysis of data. CY and XM wrote, reviewed and edited the manuscript.

Ethics approval and consent to participate

Not applicable.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Gene6991251342019

10.1016/j.gene.2019.02.058

30849543

Hibino

Saito

Muramatsu

Otani

Kasai

Kimura

Saito

Inhibitors of enhancer of zeste homolog 2 (EZH2) activate tumor-suppressor microRNAs in human cancer cells

Oncogenesis3e1042014

10.1038/oncsis.2014.17

24861464

4035694

Kim

Lee

Song

Jeong

Lee

Jeong

Hsa-miR-1246 and hsa-miR-1290 are associated with stemness and invasiveness of non-small cell lung cancer

Lung Cancer9115222016

10.1016/j.lungcan.2015.11.013

26711929

Figure 1.

Length distribution of miRNAs sequences. Length distribution of the sequenced miRNAs. Most of sequenced miRNAs exhibited 19–25 nucleotides in length. Hsa, Homo sapiens; miRNA, microRNA.

Figure 2.

Heatmap and volcano plot analysis of the differentially expressed miRNAs following cisplatin treatment. (A) Heatmap analysis of the differentially expressed miRNAs following cisplatin treatment. Red indicates upregulated miRNAs, and green indicates downregulated miRNAs. The degree of differential expression was indicated by the color intensity. (B) The volcano plot analysis of the differentially expressed miRNAs following cisplatin treatment. The image indicated that with cisplatin treatment, a total of 49 differentially expressed miRNAs were identified, including 33 upregulated and 16 downregulated miRNAs. miRNA, microRNA.

Figure 3.

Expression levels of the top five significantly upregulated and top five significantly downregulated miRNAs as identified by reverse transcription-quantitative polymerase chain reaction analysis. The expression level of hsa-miR-1246 was significantly increased, and that of hsa-miR-892b was significantly decreased in NCI-N87 cells treated with cisplatin compared with control cells. This was consistent with the results of microarray analysis. **P<0.01 vs. control. miR, microRNA.

Figure 4.

Investigation of hsa-miR-1246- and hsa-miR-892b-regulated genes by Venn diagram analysis and GO clustering. (A) The intersecting genes of hsa-miR-1246-regulated genes by Venn diagram analysis. (B) The intersecting genes of hsa-miR-1246-regulated genes analysis by GO clustering. (C) The intersecting genes of hsa-miR-892b-regulated genes by Venn diagram analysis. (D) The intersecting genes of hsa-miR-892b-regulated genes analysis by GO clustering. miR, microRNA; GO, Gene Ontology; miRDB, microRNA Target Prediction and Functional Study Database.

Figure 5.

KEGG pathway analysis of hsa-miR-1246 and hsa-miR-892b-regulated genes by histogram and cross-talk network. (A) The histogram exhibited the KEGG pathway of the hsa-miR-1246 regulated genes. (B) The cross-talk network analysis of the hsa-miR-1246 regulated genes. (C) The histogram exhibited the KEGG pathway of the hsa-miR-892b-regulated genes. (D) The cross-talk network analysis of the hsa-miR-892b-regulated genes. KEGG, Kyoto Encyclopedia of Genes and Genomes; miR, microRNA.

Table I.

Primers used for the reverse-transcription polymerase chain reaction.

Primer	Sequence (5′→3′)
hsa-miR-3609-F	5′-ACACTCCAGCTGGGCAAAGTGATGAGTAATAC-3′
hsa-miR-3609-R	5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCAGCCAGT-3′
hsa-miR-4497-F	5′-ACACTCCAGCTGGGCTCCGGGACGG-3′
hsa-miR-4497-R	5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGGCCCAGCC-3′
hsa-miR-1246-F	5′-ACACTCCAGCTGGGAATGGATTTTTGG-3′
hsa-miR-1246-R	5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCCTGCTCC-3′
hsa-miR-4301-F	5′-ACACTCCAGCTGGGTCCCACTACTTCAC-3′
hsa-miR-4301-R	5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCACAAGT-3′
hsa-miR-6724-5p-F	5′-ACACTCCAGCTGGGCTGGGCCCGCGGCGGGC-3′
hsa-miR-6724-5p-R	5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCCCCACGC-3′
hsa-miR-4508-F	5′-ACACTCCAGCTGGGGCGGGGCTGGG-3′
hsa-miR-4508-R	5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCGCGCGCC-3′
hsa-miR-33b-3p-F	5′-ACACTCCAGCTGGGCAGTGCCTCGGCAGTG-3′
hsa-miR-33b-3p-R	5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGGGGCTGCA-3′
hsa-miR-1268b-F	5′-ACACTCCAGCTGGGCGGGCGTGGTGGTG-3′
hsa-miR-1268b-R	5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCACCCCCA-3′
hsa-miR-153-5p-F	5′-ACACTCCAGCTGGGTCATTTTTGTGATGTT-3′
hsa-miR-153-5p-F	5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGCTGCAA-3′
hsa-miR-892b-F	5′-ACACTCCAGCTGGGCACTGGCTCCTTTCTG-3′
hsa-miR-892b-R	5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCTACCCA-3′
U6-F	5′-CTCGCTTCGGCAGCACA-3′
U6-R	5′-AACGCTTCACGAATTTGCGT-3′

Hsa, homo sapiens; miR, microRNA; F, forward; R, reverse.

Table II.

Microarray expression profile analysis of microRNAs in NCI-N87 gastric cancer cells following cisplatin treatment.

miR	Normalized mean value	Normalized mean value in the control group	Normalized mean value in the cisplatin treatment group	Fold change	P-value	Adjusted P-value
hsa-miR-3609	93	7	180	27.1907	3.32×10⁻⁸³	5.94×10⁻⁸¹
hsa-miR-4497	287	21	553	26.7058	7.36×10⁻¹⁶³	6.59×10⁻¹⁶⁰
hsa-miR-1246	1,530	173	2,887	16.6663	7.95×10⁻⁷¹	1.19×10⁻⁶⁸
hsa-miR-4301	15,355	1,951	28,759	14.7441	7.76×10⁻¹⁰⁰	2.32×10⁻⁹⁷
hsa-miR-6724-5p	192	25	359	14.6075	2.13×10⁻¹⁰⁸	9.51×10⁻¹⁰⁶
hsa-miR-598-5p	31	4	58	12.9564	3.79×10⁻¹⁵	6.64×10⁻¹⁴
hsa-miR-4485-3p	185	27	343	12.7338	8.22×10⁻⁰⁹	7.08×10⁻⁰⁸
hsa-miR-542-5p	6	1	12	11.6069	3.93×10⁻⁰⁸	3.00×10⁻⁰⁷
hsa-miR-647	12	2	22	9.4955	1.45×10⁻¹³	1.96×10⁻¹²
hsa-miR-6073	45,471	8,943	81,999	9.1686	9.15×10⁻⁴⁰	6.30×10⁻³⁸
hsa-miR-892a	5	1	9	9.0982	0.00074172	0.00301745
hsa-miR-3135b	8,839	1,807	15,871	8.7814	2.23×10⁻⁵²	2.00×10⁻⁵⁰
hsa-miR-589-3p	7	2	12	6.7428	8.38×10⁻⁰⁷	5.28×10⁻⁰⁶
hsa-miR-4652-3p	427	138	715	5.1846	4.70×10⁻¹⁹	9.34×10⁻¹⁸
hsa-miR-190b	33	11	55	5.1624	3.19×10⁻²¹	7.32×10⁻²⁰
hsa-miR-4796-3p	3	1	5	4.9350	0.00614119	0.01850627
hsa-miR-548t-5p	107	36	178	4.9321	3.05×10⁻⁴¹	2.27×10⁻³⁹
hsa-miR-1291	102	35	169	4.8621	2.77×10⁻³⁹	1.77×10⁻³⁷
hsa-miR-4461	32	11	52	4.8275	1.14×10⁻¹⁹	2.44×10⁻¹⁸
hsa-miR-6077	158	54	261	4.7968	2.65×10⁻³²	1.19×10⁻³⁰
hsa-miR-4780	11	4	19	4.7869	1.76×10⁻⁰⁸	1.46×10⁻⁰⁷
hsa-miR-193a-3p	11	4	19	4.7675	2.97×10⁻⁰⁸	2.31×10⁻⁰⁷
hsa-miR-3180-3p	3	1	5	4.7199	0.04119246	0.09570207
hsa-miR-6131	159	56	263	4.6951	9.21×10⁻²⁶	2.84×10⁻²⁴
hsa-miR-4474-3p	13	4	21	4.6427	1.96×10⁻⁰⁶	1.19×10⁻⁰⁵
hsa-miR-3944-3p	17	6	28	4.2961	2.18×10⁻⁰⁹	2.04×10⁻⁰⁸
hsa-miR-7107-5p	16	6	27	4.2217	9.13×10⁻⁰⁹	7.79×10⁻⁰⁸
hsa-miR-149-5p	14	5	23	4.2044	9.25×10⁻⁰⁹	7.81×10⁻⁰⁸
hsa-miR-6809-3p	3	1	4	4.1852	0.01789444	0.04669248
hsa-miR-3182	720	282	1,159	4.1092	6.49×10⁻³⁴	3.06×10⁻³²
hsa-miR-7641	52	20	83	4.0835	1.45×10⁻¹⁹	3.02×10⁻¹⁸
hsa-miR-19a-5p	39	16	63	4.0720	6.52×10⁻²⁰	1.42×10⁻¹⁸
hsa-miR-6723-5p	17	7	27	4.0387	0.00518868	0.01601334
hsa-miR-548ab	6	9	2	0.2471	0.00544169	0.01673648
hsa-miR-4496	2	4	1	0.2460	0.04127486	0.09570207
hsa-miR-95-3p	9	14	3	0.2335	5.91×10⁻⁰⁶	3.35×10⁻⁰⁵
hsa-miR-3960	3	4	1	0.2299	0.02518227	0.06285282
hsa-miR-548c-3p	9	14	3	0.2067	8.12×10⁻⁰⁶	4.41×10⁻⁰⁵
hsa-miR-181b-3p	91	152	31	0.2048	2.38×10⁻³⁷	1.42×10⁻³⁵
hsa-miR-6743-3p	3	5	1	0.1925	0.00920966	0.02633433
hsa-miR-4472	5	9	2	0.1774	0.0001624	0.00074159
hsa-miR-5000-3p	8	14	2	0.1724	2.20×10⁻⁰⁶	1.32×10⁻⁰⁵
hsa-miR-4454	169	290	47	0.1624	7.04×10⁻²²	1.75×10⁻²⁰
hsa-miR-1258	4	6	1	0.1573	0.00176885	0.00646173
hsa-miR-4508	103	179	28	0.1541	1.19×10⁻⁰⁹	1.14×10⁻⁰⁸
hsa-miR-33b-3p	18	31	5	0.1453	2.76×10⁻⁰⁸	2.23×10⁻⁰⁷
hsa-miR-1268b	267	477	57	0.1204	9.38×10⁻⁹³	2.10×10⁻⁹⁰
hsa-miR-153-5p	50	91	9	0.1019	3.28×10⁻³⁵	1.73×10⁻³³
hsa-miR-892b	20	37	3	0.0773	3.90×10⁻¹³	5.14×10⁻¹²

Hsa, homo sapiens; miR, microRNA.