Contributed equally
Corneal integrity, transparency and vision acuity are maintained by corneal epithelial cells (CECs), which are continuously renewed by corneal limbal stem cells (LSCs). Deficiency of CECs and/or LSCs is associated with numerous ocular diseases. Paired box (PAX)6 is an eye development-associated transcription factor that is necessary for cell fate determination and differentiation of LSCs and CECs. In the present study, the PAX6 gene was introduced into adipose-derived rat mesenchymal stem cells (ADMSCs) to investigate whether PAX6-transfected cells were able to transdifferentiate into corneal-like epithelial cells and to further verify whether the cells were suitable as a cell source for corneal transplantation. The ADMSCs were isolated from the bilateral inguinal region of healthy Sprague Dawley rats. The characteristics of ADMSCs were identified using flow cytometric analysis. After subculture, ADMSCs underwent transfection with recombinant plasmid containing either PAX6-enhanced green fluorescent protein (EGFP) complementary (c)DNA or EGFP cDNA (blank plasmid group), followed by selection with G418 and determination of the transfection efficiency. Subsequently, the morphology of the ADMSCs and the expression profiles of corneal-specific markers CK3/12 and epithelial-specific adhesion protein were determined. E-cadherin was detected using immunofluorescence staining and western blot analysis at 21 days following transfection. An MTT cell proliferation and a colony formation assay were performed to assess the proliferative activity and clonogenicity of PAX6-transfected ADMSCs. Finally, the PAX6-expressing ADMSCs were transplanted onto the cornea of a rabbits with limbal stem cell deficiency (LSCD). At 21 days after transfection, the ADMSCs with PAX6 transfection exhibited a characteristic flagstone-like appearance with assembled corneal-like epithelial cells, and concomitant prominent expression of the corneal-specific markers cytokeratin 3/12 and E-cadherin. Furthermore, the proliferation and colony formation ability of PAX6-overexpressing ADMSCs was significantly retarded. The transplantation experiment indicated that PAX6-reprogramed ADMSCs attached to and replenished the damaged cornea via formation of stratified corneal epithelium. Taken together, these results suggested that conversion of ADMSCs into corneal-like epithelium may be driven by PAX6 transfection, which makes ADMSCs a promising cell candidate for the treatment of LSCD.
The surface of the cornea consists of a unique type of non-keratinized epithelial cells [corneal epithelial cells (CECs)] that is essential to assure corneal transparency and acuity of vision. CECs are continuously renewed by a population of stem cells or progenitor cells that reside in the corneal limbus. However, severe corneal injury caused by chemical or mechanical injury, and immune or hereditary diseases may lead to corneal inflammation, ulceration, neovascularization and limbal stem cell deficiency (LSCD), all of which may cause blindness (
A new modality to reconstruct the ocular surface by using bioengineered CECs has emerged within the last decade, and the application of mesenchymal stem cells (MSCs) as bioengineered corneal epithelia has been attempted in numerous experimental studies (
Paired box 6 (PAX6) is a member of the PAX gene transcription factor family that is an earlier differentiation marker expressed by CECs, and the main driver of the differentiation of CECs (
In the present study, the differentiation capacity of ADMSCs into corneal epithelial-like cells driven by PAX6 was investigated
Dulbecco's modified Eagle's medium (DMEM)/F12, 10% fetal bovine serum (FBS) and type I collagenase were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany) Anti-rat CD34-Fluorescein isothiocyanate (FITC; cat no. sc-7324) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); CD90-FITC (cat no. 206105) and CD45-FITC (cat no. 202205) were purchased from BioLegend Inc. (San Diego, CA, USA); and CD105-Phycoerythrin (PE; cat no. FAB10971P) was purchased from BD Biosciences (Franklin Lakes, NJ, USA).
Rabbit anti-PAX6 polyclonal antibody (cat no. ab5790), mouse anti-cytokeratin (CK) 3/12 monoclonal antibody (cat no. ab68260) and mouse anti-E-cadherin (cat no. ab76055) were obtained from Abcam (Cambridge, MA, USA). Horseradish peroxidase-conjugated mouse anti-rabbit (cat no. sc-2357) and donkey anti-mouse antibody (cat no. sc-2314) were purchased from Santa Cruz Biotechnology. PAX6-enhanced green fluorescence protein (EGFP) complementary (c)DNA plasmid [item no. CCS-Mm04345-PCDNA3.1(+)] was constructed by Guangzhou FulenGen Co., Ltd. (Guangzhou, China), where the PAX6 gene inserted had a GenBank accession no. of NM_013627 and an open reading frame length of 1,311 bp (
This study was approved by the Animal Experimentation Ethics Committee of Chengdu Medical College (Chengdu, China). Animal experiments were performed in a manner consistent with the Statement for the Use of Animals in Ophthalmic and Vision Research.
A total of 20 male Sprague Dawley (SD) rats aged 6 weeks with a mean body weight of 220±10 g and 10 male New Zealand white rabbits aged 6 months with an initial body weight of 2.0±0.4 kg were purchased from the Experimental Animal Center of Sichuan University (Chengdu, China). The animals were housed at an ambient temperature of 25±2°C and a relative humidity of 65–70% under a 12-h light/dark cycle. All animals were fed according to the Specific Pathogen Free Animal Criteria, and were allowed free access to food and water.
The ADMSCs were isolated from bilateral inguinal fat pads of healthy SD rats. Adipose tissue were washed extensively with sterile PBS and minced into pieces of 2 mm3 with fine scissors. The tissue was digested with 0.1% type I collagenase for 1 h at 37°C. After partial digestion, the collagenase activity was neutralized by adding DMEM/F12 supplemented with 10% FBS followed by centrifugation at 500 × g for 10 min at 4°C. The resulting cell suspension was filtered through a 100-mm cell strainer and centrifuged at 500 × g for 5 min at 4°C. Cell pellets were resuspended in DMEM/F12 supplemented with 10% FBS, and incubated at 37°C in a 5% CO2 incubator. After 72–96 h, non-adherent cells were discarded and the medium was changed every 2–3 days. Cells were maintained in culture until they reached 80% confluence and were examined daily using an inverted fluorescence microscope. The ADMSCs at the third passage were trypsinized, washed with PBS and incubated with antibodies against CD34, CD45, CD90 and CD105(1:100) for 30 min at 4°C. Flow cytometry detection was performed using a BD Accuri C6 flow cytometer (BD Biosciences).
ADMSCs at the third passage were seeded into 24-well plates at a density of 0.8×105 cells/well, setting 3 replicates for each cell clone. After being cultured for 24 h in antibiotic-free DMEM/F12 containing 10% FBS, the cells were ~95% confluent. The cultured ADMSCs were respectively subjected to transfection with recombinant PAX6-EGFP cDNA or EGFP cDNA plasmid using Lipofectamine® 2000 transfection reagent according to the manufacturer's instructions. The cells were transfected at 37°C in a CO2 incubator for 24 h prior to testing for transgene expression. The cells were then passaged at 1:10 dilution in fresh growth medium. The following day, the cells were selected in G418-containing medium (600 µg/ml) for 7 days. Thirty days later, when cell death ceased and proliferation occurred thereafter, 800 µg/ml G418 was replaced with 400 µg/ml G418 for maintenance. After 14 days, the stable G418-resistant clones with good growth were isolated and subcultured. The resultant clusters were observed and images were captured under an inverted fluorescence microscope. Subsequently, the expression of marker proteins was determined in the stably transfected cells at the third passage via immunofluorescence staining and western blot analysis.
Cells cultured on sterile glass cover slips were washed briefly with PBS and fixed in a mixture of methanol and acetone for 15 min at room temperature. The samples were then blocked with 10% normal blocking serum in PBS for 20 min and incubated overnight with anti-CK3/12 or E-cadherin primary antibody. After washing 3 times with PBS, the samples were incubated in a dark chamber with fluorochrome-conjugated secondary antibody for 2 h. The samples were extensively washed with PBS and cover slips were mounted with 90% glycerol in PBS.
Cells in each group were seeded at 4×105 cells/well into 96-well plates. After 24 h of incubation, the cells were harvested, washed and lysed in 1.0 ml of pre-cooled radioimmunoprecipitation assay buffer with freshly added protease inhibitors (Sigma-Aldrich; Merck KGaA) on ice for 30 min with vortexing every 10 min. Following centrifugation at 12,000 × g for 30 min at 4°C, the supernatant was subjected to BCA protein quantification (Thermo Fisher Scientific, Inc.). Equal amounts of total protein (30 µg per lane) were subjected to 10% SDS-PAGE and transferred onto a nitrocellulose membrane (Bio-Rad Laboratories, Inc., Hercules, CA, USA), which was then blocked with 5% non-fat dry milk in Tris-buffered saline containing Tween 20 (TBS-T) for 2 h at room temperature. The membrane was then incubated overnight at 4°C with anti-PAX6 (1:500), anti-CK3/12 (1:50) and anti-E-cad (1:500). Following washing three times with TBS-T for 20 min each, the membrane was incubated with a horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (1:2,000; Santa Cruz Biotechnology, Inc) in 1% bovine serum albumin (BSA; Gibco; Thermo Fisher Scientific, Inc.). Following washing as above, the membrane was incubated with enhanced chemiluminescence solution (Thermo Fisher Scientific, Inc) for 4 min and covered with a transparent sheet. Films were exposed and evaluated using a LAS-3000 (Fujifilm, Tokyo, Japan) imaging system.
The MTT assay was performed following the manufacturer's instructions. In brief, stably transfected cells were seeded in 96-well culture plates at 104 cells/well and grown in DMEM/F12 at 37°C for 48 h. Cells were incubated with MTT, and formazan crystals were solubilized in dimethyl sulfoxide (DMSO). Absorbance was measured at 570 nm using a Synergy 2 multidetection microplate reader (BioTek, Winooski, VT, USA). The growth curve was created based on the results.
The cells at passage 3 from each group were seeded onto 6-well plates at a density of 2,000 cells/well in three replicates. Fresh basic growth medium was added to each well following incubation for 12 days at 5% CO2 and 37°C. Finally, the culture medium was aspirated, each well was washed with PBS and topped up with 1 ml 0.5% crystal violet solution (0.5%), followed by incubation for 10 min at room temperature. Thereafter, each well was washed with a gentle stream of water under running tap water. The number of ‘darkly’ stained colonies was counted under a phase-contrast microscope at low magnification. Values are expressed as colony-forming efficiency calculated as the percentage of the ratio of the number of colonies counted vs. the number of cells initially seeded.
A total of 10 male New Zealand white rabbits aged 6 months with an initial body weight of 2.0±0.4 kg were purchased from the Experimental Animal Center of Sichuan University (Chengdu, Sichuan province, China). The animals were housed at an ambient temperature of 25±2°C and a relative humidity of 65–70% under a 12-h light/dark cycle. All animals were fed according to the Specific Pathogen Free Animal Criteria, and were allowed free access to food and water. All rabbits were anaesthetized with 50 mg/kg of 1% pentobarbital sodium, administered intraperitoneally. The ocular surface was briefly exposed to 5% iodine-povidone/95% PBS, and the left eyes were subjected to a radical 360° limbal peritomy as previously described by Selver
At 24 h after the establishment of the LSCD model, the 10 rabbits were randomly divided into the control group (n=5) and transplantation group (n=5), which were subjected to vehicle treatment or engraftment of EGFP-labeled PAX6+ ADMSCs, respectively. Prior to transplantation, all rabbits were anaesthetized as described above for establishing the LSCD model. The transplantation was performed based on the method by Ueno
Seven days following transplantation, the 10 rabbits were anaesthetized by intraperitoneal injection of 1% pentobarbital sodium at the dose of 50 mg/kg and then sacrificed by an air embolism. The specimens used for pathological examinations were fixed in 10% formaldehyde for 24 h at room temperature, dehydrated using a gradual alcohol series, embedded in paraffin, and sectioned into 30 µm thick sections. Subsequently, the sections were subjected to histological examination with hematoxylin and eosin (H&E), as well as CK3/12 immunochemistry to determine the effect of cell engraftment. Corneas were fixed in formalin, embedded in paraffin and sectioned at 4 µm. For histological staining, slides were refixed, washed and stained with hematoxylin for 5 min and eosin for 3 min at room temperature, respectively. Immunostaining of the corneal sections involved deparaffinization and rehydration in PBS, permeabilization with 1% Triton X-100 for 10 min, blocking with 1% BSA for 1 h, overnight incubation at 4°C with mouse anti-CK3/12 monoclonal antibody (1:100), three 5-min washes with PBS, incubation for 1 h with Alexa fluor-594-conjugated anti-mouse immunoglobulin G (1:400; cat no, A-11032, Invitrogen; Thermo Fisher Scientific, Inc.). and three 5-min washes. Sections were counterstained by incubation with 0.5 µg/ml DAPI for 10 min, followed by immunofluorescence microscopy.
Values are expressed as the mean ± standard deviation. Statistical analysis was performed using SPSS v.15.0 software for Windows (SPSS, Inc., Chicago, IL, USA). Statistical significance was assessed by a Student's t test. P<0.05 was considered to indicate a statistically significant difference.
Positive expression of the MSC markers CD105 and CD90 and negative expression of the hematopoietic lineage markers CD34 and CD45 was confirmed in third-passage ADMSCs. This result indicated the purity and successful expansion of MSCs
First, the morphology of cultured ADMSCs and the transfection efficiency with EGFP-expressing plasmids was observed by light and florescence microscopy, respectively, and the expression of Pax6 and EGFP was evaluated using western blot analysis at 24 h after PAX6 transfection. As expected, successful transfection of PAX6 and EGFP was observed in ADMSCs, as evidenced by green fluorescence emitted from the ADMSCs transfected with EGFP control or PAX6 expression plasmid under a florescence inverted microscope (
At 24 h after PAX6 transfection, ADMSCs presented as uniformly long, fusiform shaped, assembling fibroblasts (
The growth curve created based on the MTT cell viability assay unveiled that PAX6 overexpression significantly retarded the proliferation of PAX6-reprogrammed ADMSCs at 21 days after transfection (
In the LSCD model, the central corneal epithelium was thoroughly scraped off from the cornea and the whole limbus was removed. Seven days following transplantation of PAX6-expressing ADMSCs, the eyeballs were excised and sectioned at 30 µm. The sections were subjected to histological examination with H&E and CK3/12 immunofluorescence staining. HE staining revealed that the injured cornea transplanted with the PAX6-transfected cells demonstrated an intact morphology of the corneal surface with stratified epithelial cells (
The integrity of the corneal surface is maintained by CECs continuously renewed by LSCs. Severe ocular disorders usually result from CEC deficiency and/or LSCD, leading to failure of epithelial regeneration or non-transparent cornea, causing corneal surface disease that leads to blindness (
PAX6 is a homeobox transcription factor acknowledged to have a critical role in corneal epithelial cell fate determination and differentiation of LSCs and CECs. Previously, Ueno
As expected, results of the present study proved for the first time that PAX6 induced ADMSCs to transdifferentiate into corneal-like epithelial cells
Furthermore, MTT and colony forming units assays demonstrated that the viability and proliferative capacity of the ADMSCs was retarded by PAX6 transfection, which was consistent with the results of Bardag-Gorce
Following successful transfection of the PAX6 expression plasmid into rat ADMSCs using lipofection followed by G418 selection, corneal epithelial-like cells were yielded. Subsequently the EGFP-labeled PAX6+ ADMSCs were transplanted onto the damaged cornea of rabbits in an
In conclusion, the results of the present study demonstrated that PAX6 induces ADMSCs to differentiate into corneal epithelial-like cells
This study was supported by the Scientific Research Fund of Sichuan Provincial Education Department (grant nos. 15ZA0266 and 17ZB0122), Scientific Research Project of the Health and Family Planning Commission of Sichuan Province (grant nos. 16PJ104 and 16ZD038) and Scientific Research Project of the Development and Regeneration Key Laboratory of Sichuan Province (grant nos. SYS15-004 and SYS15-008).
(A) Map of the construction protocol for the PAX6/EGFP-His expression plasmid. PAX6-EGFP cDNA plasmid [item no. CCS-Mm04345-PCDNA3.1(+)] was constructed by Guangzhou FulenGen Co., Ltd. The PAX6-EGFP fusion gene void of the termination codon of the PAX6 gene was inserted between the HindIII and XbaI sites in the backbone of the pCDNA3.1/His vector. The pCDNA3.1/His vector contained a neomycin-resistance gene for G418 selection with SV40 as the driver. The PAX6 gene had a primary GenBank accession no. of NM_013627 and an open reading frame length of 1,311 bp. (B) Schematic of the experimental protocol. ADMSCs were transfected with the PAX6 gene using Lipofectamine. The transfected cells were cultivated with G418-containing media for 7 days. The resultant stably PAX6 expression vector-transfected cells were transplanted onto the cornea of a rabbit limbal stem cell deficiency model. Seven days after transplantation, the repair potential of the PAX6-reprogramed ADMSCs for corneal epithelial defects was evaluated. EGFP, enhanced green fluorescence protein; PAX6, paired box 6; ADMSCs, adipose-derived mesenchymal stem cells.
Flow cytometric analysis of ADMSCs. Expression of CD105, CD90, CD34 and CD45 was detected in ADMSCs isolated from bilateral inguinal fat pads of healthy Sprague Dawley rats. The cells at the third passage were incubated with FITC-labeled monoclonal antibodies against CD90 and CD45, or PE-labeled CD34 and CD105 for 30 min at 4°C and analyzed by flow cytometry to determine the surface marker expression of the ADMSCs. The control samples were not labeled with antibodies. The ADMSCs were positive for the mesenchymal stem cell markers CD90 and CD105 (A, green line; B, red line), and negative for the hematopoietic marker CD34 and CD45 (C, green line; D, red line). The blue line represents the control. ADMSCs, adipose-derived mesenchymal stem cells; FITC, fluorescein isothiocyanate; PE, phycoerythrin.
Stable PAX6 transfection into rat ADMSCs. (A) Fluorescence microscopic images of cultured ADMSCs with PAX6 transfection observed at 24 h after transfection. Scale bar, 100 µm. Left panels, phase contrast photomicrographs displaying fibroblasts assembling as uniformly long and fusiform shapes. Center panels, inverted florescence microscopy images; PAX6 expression indicated by green fluorescence was observed to be mainly located in the nuclei. Right panels, merged fluorescence and phase contrast images. (B) Validation of the transfection efficiency of EGFP and PAX6 by western blot analysis. Western blot results demonstrated that EGFP and PAX6 were present in the PAX6 expression vector-transfected ADMSCs, whereas PAX6 were absent in the control plasmid group. EGFP, enhanced green fluorescence protein; PAX6, paired box 6; ADMSCs, adipose-derived mesenchymal stem cells; cDNA, complementary DNA.
Characterization of the PAX6-transfected ADMSCs. (A) Fluorescence microscopic images of cultured EGFP-labeled PAX6+ ADMSCs observed at 21 days after transfection. The EGFP-labeled PAX6+ ADMSCs exhibited a characteristic flagstone-like appearance of assembling corneal epithelial cells. However, no obvious morphological alteration was seen in the blank plasmid group. (B) Immunofluorescence staining of corneal-specific markers CK3/12 and E-cadherin. Expression of CK3/12 and E-cadherin was observed in the PAX6-overexpressing ADMSCs (scale bars, 100 µm). (C) Western blot analysis of the corneal-specific markers CK3/12 and E-cadherin. High expression of CK3/12 and E-cadherin was revealed in the PAX6-overexpressing ADMSCs, while it was absent in ADMSCs of the blank plasmid group. (D) A cell growth curve was obtained by performing an MTT cell viability assay. (E and F) Colony formation assay of ADMSCs. The colony-forming efficiency in the PAX6+ ADMSCs was ~60% of that in the blank plasmid group. The difference in colony-forming efficiency between the two groups was statistically significant. Values are expressed as the mean ± standard error of the mean (n=3). *P<0.05, **P<0.01. EGFP, enhanced green fluorescence protein; PAX6, paired box 6; ADMSCs, adipose-derived mesenchymal stem cells; OD, optical density; cDNA, complementary DNA; CK, cytokeratin.
Cell engraftment and corneal epithelium repair in a rabbit model of limbal stem cell deficiency. (A) The left eyes were subjected to a radical 360° limbal peritomy. The whole limbus with an extended dissection toward to the sclera was removed, the peripheral cornea was subjected to superficial keratectomy and the central corneal epithelium was thoroughly scraped off from the cornea. (B) The precultured EGFP-labeled PAX6+-ADMSCs at a concentration of 1×107 cells/ml were transplanted onto the damaged corneal surface using a small tube and allowed to adhere to the damaged cornea for 1 h in the transplantation group. (C-E) Seven days after transplantation, the eyeballs were excised and sectioned at 30 µm. The sections were subjected to histological examination with H&E and CK3/12 immunofluorescence staining. (C) H&E staining demonstrated that the injured cornea transplanted with the PAX6-transfected cells featured a continuous corneal surface with stratified epithelial cells. (D and E) Immunofluorescence staining of CK3/12 in the cornea at 7 days after engraftment. The confocal images of the damaged cornea subjected to transplantation with EGFP-labeled PAX6+-expressing ADMSCs revealed (D) attachment of EGFP-positive cells to the impaired corneal surface, with (E) concomitant expression of corneal epithelial cell-specific marker CK3/12 (scale bars, 100 µm). EGFP, enhanced green fluorescence protein; PAX6, paired box 6; ADMSCs, adipose-derived mesenchymal stem cells; H&E, hematoxylin and eosin; CK, cytokeratin.