OL Oncology Letters 1792-1074 1792-1082 D.A. Spandidos 10.3892/ol.2018.7909 OL-0-0-7909 Articles Overexpression of peroxiredoxin-3 and −5 is a potential biomarker for prognosis in endometrial cancer ByunJung Mi 1 2 * KimSu Sun 1 * KimKi Tae 1 2 KangMi Seon 3 JeongDae Hoon 1 2 LeeDae Sim 1 2 JungEun Jung 1 KimYoung Nam 1 2 HanJin 4 SongIn Sung 4 LeeKyoun Bok 1 2 SungMoon Su 1 2 Department of Obstetrics and Gynecology, Inje University Busan Paik Hospital, Busan 614-735, Republic of Korea Paik Institute for Clinical Research, Inje University Busan Paik Hospital, Busan 614-735, Republic of Korea Department of Pathology, Inje University Busan Paik Hospital, Busan 614-735, Republic of Korea Department of Physiology, Inje University Busan Paik Hospital, Busan 614-735, Republic of Korea Correspondence to: Professor Ki Tae Kim, Department of Obstetrics and Gynecology, Inje University Busan Paik Hospital, 75 Bokji-ro, Busanjin-gu, Busan 614-735, Republic of Korea, E-mail: hellojungmi@hanmail.net Professor Mi Seon Kang, Department of Pathology, Inje University Busan Paik Hospital, 75 Bokji-ro, Busanjin-gu Busan 614-735, Republic of Korea, E-mail: pathmsk@hanmail.net

Contributed equally

 04 2018 31 01 2018 15 4 5111 5118 16082016 24082017 Copyright © 2018, Spandidos Publications 2018

Endometrial cancer is the sixth most common cancer in women worldwide. Peroxiredoxins (PRDXs) are antioxidant enzymes that serve important roles in cell differentiation, proliferation, and apoptosis. In the present study, the potential associations between PRDX expression and endometrial cancer were investigated. The expression levels of various PRDX mRNAs were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) in endometrial cancer tissues (n=26) and normal endometrial tissues (n=10). Additionally, the expression of PRDX isoforms was immunohistochemically examined in endometrial cancer tissues and adjacent normal endometrial tissues from 42 patients. Finally, the associations between high PRDX expression levels and clinicopathological features were examined in patients with endometrial cancer. Analysis of PRDX expression in endometrial cancer tissues and normal endometrial tissues by semi-quantitative RT-PCR showed that all PRDX isoforms had increased expression in the endometrial cancer tissues compared with that in the normal endometrium, and the differences in the expression levels of PRDX1 and PRDX3 between cancer and normal tissues were statistically significant (P=0.0015 and P=0.0134, respectively). Additionally, analysis of PRDX expression in endometrial cancer and paired normal endometrial tissues by immunohistochemistry showed strong cytoplasmic staining of PRDX3 and PRDX5 in cancer tissues, with high PRDX3 (25/42, 59.5%) and PRDX5 (32/42, 76.2%) appearing more frequently in endometrial cancer than in normal endometrial tissues (P=0.0001 and P=0.0023, respectively). Furthermore, high expression of PRDX5 was associated with advanced-stage endometrial cancer (P=0.0399). Although the 5-year survival rate was marginally higher in patients with low expression of PRDX3 and PRDX5, this result was not statistically significant. In summary, PRDX3 and PRDX5 are highly expressed in endometrial cancer and could be associated with advanced stage and poor prognosis. Therefore, these proteins may potentially be used as prognostic markers for endometrial cancer.

 endometrial cancer prognostic marker peroxiredoxin Introduction

Endometrial cancer is the sixth most common cancer in women worldwide; approximately 320,000 new cases were diagnosed in 2012 (1). The incidence rate and number of mortalities from endometrial cancer are increasing, despite improvements in overall survival rates (2). Overall survival rates for endometrial cancer are good, and the 5-year survival rate for disease confined to the uterus is as high as 96% (3). In the majority of cases, patients with endometrial cancer exhibit symptoms that include abnormal vaginal bleeding or discharge, and ~90% of women diagnosed with endometrial cancer have abnormal uterine bleeding (4). Therefore, in pre- and post-menopausal women exhibiting abnormal uterine bleeding, transvaginal ultrasonography and endometrial biopsy should be performed for the potential early diagnosis of endometrial cancer (5). Early detection of endometrial cancer improves prognosis; therefore, improved diagnostic methods are critical for patients with symptoms of endometrial cancer, and screening methods for the detection of early-stage cancer prior to the onset of symptoms are expected to achieve meaningful improvements in overall survival. However, there are currently no routine, effective screening tests for endometrial cancer (6).

Tumor markers may be secreted by tumors at levels in excess of those secreted by normal tissues and cells (7). Such tumor markers can include unique extracellular matrix or cell adhesion molecules, growth factors, receptors, cytokines, or products of abnormal metabolic processes. In addition, antibodies produced by the body against tumor markers may themselves be used as markers. Tumor markers can be used as indicators of diagnosis, prognosis, and can informative for clinical management and follow-up (7). Numerous investigations have assessed different biological variables in tissue and serum samples from patients with endometrial cancer, in order to detect possible biomarkers that could be predictive of clinical outcomes. For example, Gadducci et al (8) reported the ability of different tissue and serum biomarkers, including p53, the PENT-PIK3-mTOR signaling pathway, MSI, β-Catenin, the Ras-MAPK-ERK signaling pathway and VEGF in tissue and CA 125, CA15-3, YKL-40 VEGF and HE-4 in serum, to predict clinical outcomes in patients with endometrioid-type endometrial carcinoma. Elevated serum CA125 levels have been detected in 11–43% of patients with endometrial cancer (9,10). Investigators have shown that elevated CA125 levels are able to predict extrauterine lesions, large tumor size, invasion of the lymphovascular space and deep myometrium, involvement of the cervix and adnexa, positive cytology, lymph node metastasis, and the requirement for adjuvant treatment (11). However, in patients with pure endometrioid-type endometrial cancer who have undergone adjuvant therapy and in patients with serous papillary carcinoma, the role of serum CA125 is controversial (12). Additionally, CA125 has limited utility for monitoring the response to chemotherapy and may not predict recurrence in the absence of other clinical signs (13). Therefore, additional studies are required to identify novel tumor markers for more accurate detection and management of endometrial cancers.

Peroxiredoxins (PRDXs), which were first discovered ~25 years ago (14), are a family of 22-to 27-kDa, non-selenium-dependent glutathione peroxidases that destroy peroxides, organic hydroperoxides and peroxynitrite (15). The PRDX gene family (16) includes six isoforms in mammals; these isoforms can be classified into three subclasses: Typical 2-cysteine PRDXs (PRDXs 1–4), atypical 2-cysteine PRDX (PRDX5), and atypical 1-cysteine PRDX (PRDX6) (17). PRDXs are associated with cell proliferation, apoptosis, differentiation, and gene expression in vitro (18). Notably, high expression levels of PRDXs are associated with increased resistance to radiation and certain chemotherapeutics, whereas PRDX deficiency can sensitize cells to chemotherapy and apoptosis (19).

In addition, oxidative metabolism of estrogen and the subsequent formation of reactive oxygen species (ROS) are important estrogen-related carcinogenic mechanisms (20). Felty et al (21) suggested that physiological estrogen concentrations could induce significant oxidative stress in vitro and that estrogen-induced ROS formation occurs in the mitochondria. For example, in estrogen receptor (ER)-positive breast cancer, which is a major estrogen-dependent cancer, estrogens are important cellular ROS inducers (22). Additionally, published data suggest that PRDX1, PRDX3, PRDX4 and PRDX5 expression levels are associated with prognosis in patients with breast cancer (23–25). Similarly, endometrial cancer is an estrogen-dependent malignancy; therefore, certain PRDXs may be associated with the prognosis or clinicopathological characteristics of endometrial cancer.

Accordingly, in the present study, the expression levels of various PRDXs were evaluated in endometrial cancer tissues, and the relationship between PRDX expression and prognosis in patients with endometrial cancer was investigated.

 Materials and methods <sec> <title>Tissues

Fresh tissue specimens were collected from the endometrial tissues of 70 patients who underwent hysterectomy at Inje University Busan Paik Hospital (Busan, Korea) between January 2008 and December 2010. Using a retrospective chart review, a database was established containing information regarding prognostic factors, such as age, body mass index, histopathological factors, stage, recurrence, and survival. Tissue specimens were classified into two groups: Normal endometrium and endometrial cancer. 42 patients with endometrial cancer received full staging surgery, including total hysterectomy, bilateral salpingo-oophorectomy, omentectomy, bilateral pelvic lymphadenectomy and para-aortic lymphadenectomy. Endometrial carcinomas are graded by their architecture and revised International Federation of Gynecology and Obstetrics (FIGO) classification (26) is used for their staging. Normal endometrial tissues were obtained from hysterectomies performed for benign uterine disease, including uterine myoma, adenomyosis, or uterine prolapse.

This study was performed following the acquisition of informed consent from all patients and approval from the Institutional Review Board of Busan Paik Hospital.

 RNA isolation and cDNA synthesis

Total RNA was isolated from tissue samples using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer's protocol. cDNA was synthesized from 1 µg total RNA using a TOPscript™ cDNA Synthesis Kit (Enzynomics, Daejeon, Korea). The reverse transcription (RT) reaction was performed at 42°C for 60 min in a reaction mixture containing 10X TOPscript™ RT reaction MIX (dT18) and TOPscript™ Reverse TranscriptaseR+ (200 U/µl) in a total volume of 20 µl.

 Semi-quantitative RT-polymerase chain reaction (PCR)

The RT product (1 µl) was used for PCR. Each reaction contained 10 pM of each primer, 1.5 mM MgCl2, 250 µM dNTPs, 10 mM Tris-HCl (pH 9.0), 30 mM KCl, and 1 unit Top DNA polymerase (AccuPower® PCR PreMix; cat. no. K-2012; Bioneer Corporation, Daejeon, Korea). The primers utilized are presented in Table I. Reactions were performed on a T100 Thermal Cycler (Bio-Rad, Hercules, CA, USA). Amplification was conducted with the following thermal cycling conditions: 5 min at 95°C; 35 cycles of amplification consisting of 30 sec at 95°C, 40 sec at 55°C, and 30 sec at 72°C; and a final extension at 72°C for 5 min. For analysis, 5 µl of the product was subjected to agarose gel electrophoresis and visualized by ethidium bromide. PCR bands were quantified using the Multi Gauge V2.2 software program (FUJI PHOTO FILM, Japan).

 Immunohistochemistry

Paraffin block was provided at Inje University Busan Paik Hospital (Busan, Korea). Immunohistochemical analysis was performed on 4-µm-thick paraffin-embedded sections of 42 tissues, which were mounted on SuperFrost Plus slides. Slides were heated for 1 h at 60°C, deparaffinized in xylene, rehydrated in graded alcohol and rinsed in distilled water. Antigen retrieval and immunohistochemistry were performed using either a Benchmark XT or Discovery XT automated immunohistochemistry system (Ventana Medical Systems, Inc., Tucson, AZ) with an OptiView DAB IHC Detection kit. Slides were incubated for 34 min at room temperature with diluted primary antibody. The appropriately diluted primary antibodies were as follows: PRDX1 (Abcam, Cambridge, MA, USA; cat. no. ab109506; dilution, 1:500), PRDX3 (Abcam; cat. no. ab16751; dilution, 1:1,000), PRDX5 (Abcam; cat. no. ab127922; dilution, 1:200), and PRDX6 (Abcam; cat. no. ab133348; dilution, 1:700). After antibody staining, sections were washed in distilled water, lightly counterstained with hematoxylin, rehydrated and mounted with coverslips. The intensity of immunohistochemical staining was evaluated in five randomly selected high-power fields using a light microscope (magnification ×400). Sections were subsequently divided into four categories: -, no positive cells; +, weak cytoplasmic staining; ++, moderate cytoplasmic staining; +++, strong cytoplasmic staining. The results were independently assessed by one investigator who was blinded to patient characteristics, stage and prognosis.

 Statistical analysis

Statistical analyses were performed using MedCalc version 14.8.1 (Frank Schoonjans, Ghent University, Belgium). Categorical variables were compared using χ2 tests and Fisher's exact tests. The mean, median, and standard deviation were calculated for continuous variables and were compared using Mann-Whitney U-tests for two groups, Kruskal-Wallis tests for three or more unmatched groups, and Pearson's correlation coefficients. Survival analysis was performed by Kaplan-Meier analysis and generalized log-rank tests. P<0.05 was considered to indicate a statistically significant difference.

 Results <sec> <title>Measurement of PRDX expression levels in endometrial cancer and normal endometrial tissues by RT-PCR

The expression levels of various PRDX mRNAs were analyzed in endometrial cancer (n=26) and normal endometrial tissue (n=10) to determine whether PRDX expression was associated with endometrial cancer. All PRDX mRNAs were upregulated in endometrial cancer compared with normal endometrial tissue (Fig. 1). Although the expression levels of PRDX2, PRDX4 and PRDX5 mRNAs were higher in endometrial cancer than in normal endometrium, these differences were not significant. Additionally, the expression level of PRDX6 mRNA in endometrial cancer was marginally higher than that in normal endometrium (0.52 vs. 0.69, respectively; P=0.0612). Notably, the expression levels of PRDX1 (0.57 vs. 0.76, respectively; P=0.0015) and PRDX3 (0.49 vs. 0.64, respectively; P=0.0134) were significantly increased in endometrial cancer compared with those in normal endometrial tissues (Fig. 1).

 Measurement of PRDX expression levels in endometrial cancer and paired normal endometrial tissues by immunohistochemistry

The expression levels of PRDX proteins were compared between endometrial cancer and paired normal endometrial tissues using immunohistochemistry. Based on the RT-PCR analysis, which detected marked changes in the expression of PRDX1, PRDX3, and PRDX6 mRNAs, and a previous study in which PRDX5 was found to be overexpressed in endometrial cancer (27), immunohistochemical staining for PRDX1, PRDX3, PRDX5, and PRDX6 was performed. The results showed that 59.5% of endometrial cancer samples and 31.6% of normal endometrial samples showed strong cytoplasmic staining for PRDX3, and statistical analysis revealed that PRDX3 was significantly overexpressed in endometrial cancer compared with normal endometrial tissue (P=0.0001; Table II; Fig. 2A). Similarly, a total 76.2% of endometrial cancer samples and 55.9% of normal endometrial tissues were strongly positive for PRDX5, and PRDX5 was significantly overexpressed in endometrial cancer compared with that in endometrial tissues (P=0.0023; Table II; Fig. 2B). However, no significant differences in the expression of PRDX 1 and 6 were observed between cancer and normal tissue.

 Analysis of the association between PRDX3/PRDX5 overexpression and prognosis in patients with endometrial cancer

The associations between PRDX isoform expression and prognosis were evaluated in patients with endometrial cancer in order to investigate the usefulness of PRDX as a prognostic biomarker for endometrial cancer. As PRDX3 and PRDX5 were demonstrated to be overexpressed in endometrial cancer by immunohistochemistry, prognostic factors for endometrial cancer were investigated according to PRDX3 and PRDX5 expression. Patients were divided into the low (weak or moderate staining) and high (strong staining) PRDX3 and PRDX5 expression groups, and clinicopathological and prognostic variables of endometrial cancer, including grade, histology, FIGO stage, depth of myometrial invasion and lymph node status were compared between the low and high expression groups of each marker.

High PRDX5 expression was more frequently observed in advanced-stage cancer than in early-stage cancer (100 vs. 66.7%; P=0.0399). Additionally, high expression of PRDX5 tended to be associated with the presence of lymph node metastasis; however, this association was not statistically significant (100 vs. 68.7%; P=0.0838). A total of 6 cases of recurrent cancer were reported, and all showed high expression of PRDX5. Additionally, 8 patients with endometrial cancer died during follow-up, and the majority of these patients exhibited high expression of PRDX3 (75% vs. 55.9% of patients who survived) and PRDX5 (87.5% vs. 73.5% of patients who survived); however, no significant associations were detected (Table III).

Although the 5-year survival rate was increased in the low PRDX3 expression group compared with that in the high PRDX3 expression group (88.2 vs. 76.0%, respectively), this association was not significant (P=0.2971; Fig. 3A). Additionally, the 5-year survival rate in the low PRDX5 expression group was non-significantly higher than that in the high PRDX5 expression group (90 vs. 78.1%; P=0.3818; Fig. 3B).

 Discussion

PRDX pathways are used by cells as enzymatic antioxidant defense systems in order to prevent oxidative and nitrosative damage caused by the presence of ROS (28). Among the six isoforms of PRDX (PRDX1-6), PRDX3 is localized in the mitochondria and acts as a mitochondrial scavenger of hydrogen peroxide, which protects mitochondria against oxidative damage and affects diverse cellular processes, including growth, differentiation, carcinogenesis and apoptosis (29). PRDX5 is localized in the cytosol, mitochondria, peroxisome and nucleus, and is able to reduce hydrogen peroxide, alkyl hydroperoxides, and peroxynitrite. PRDX5 is also able to use cytosolic and mitochondrial thioredoxins as physiological electron donors (30). These proteins are overexpressed in several types of malignancy, including breast cancer, mesothelioma, lung cancer, cervical cancer, prostate cancer, and multiple myeloma (31). Mitochondria in cancer cells are known to contain high levels of PRDX3 and PRDX5 (32–36), and Song et al (37) reported that the mitochondrial PRDX3 antioxidant system, which is exclusively present in mitochondria, may be a potential target for cancer therapy.

In the present study, the mRNA levels of all PRDX isoforms were higher in endometrial cancer than in normal endometrial tissue, with significant increases observed for PRDX1 and PRDX3 mRNAs. However, on immunohistochemical analysis, PRDX3 and PRDX5 were clearly elevated in the majority of endometrial cancer samples, with PRDX3 protein showing significantly increased expression. Although PRDX1 mRNA expression was significantly higher in endometrial cancer, PRDX1 protein levels tended to be lower in endometrial cancer than in the normal endometrium. This difference between the mRNA and protein levels of PRDX1 may be due to the presence of extracellular PRDXs, such as macrophage PRDX or secreted PRDXs (38). As PRDX3 and PRDX5 were found to be highly expressed in endometrial cancer by RT-PCR and immunohistochemistry, these targets may be considered biomarkers associated with endometrial cancer.

Generally, factors associated with the prognosis of endometrial cancer include FIGO stage, histology, tumor grade, depth of myometrial invasion, lymphovascular space invasion, and lymph node status (39). In this study, high expression of PRDX5 was associated with advanced-stage endometrial cancer. Although not statistically significant, high expression of PRDX5 was also observed more frequently in patients with lymph node metastasis, and overexpression of PRDX3 and PRDX5 appeared more frequent in patients who died during follow-up.

The lack of significance among these findings could be explained by the small sample size and the limited stages and histological characteristics of the samples. Indeed, the majority of the samples were from patients with early-stage endometrial cancer and low-grade tumors. Therefore, more meaningful results may be obtained in studies with larger sample sizes and tumors with various clinicopathological characteristics.

In conclusion, PRDX3 and PRDX5 were highly expressed in endometrial cancer. In particular, the increased expression of PRDX5 was significantly associated with advanced stage, and tended to be increased among patients with positive lymph node status. Although no significant differences were detected in this analysis, the increased expression of PRDX3 and PRDX5 may be associated with decreased survival time. Therefore, these proteins may be candidate prognostic markers in patients with endometrial cancer. Additional studies with larger sample sizes are required to fully determine the prognostic roles of PRDX3 and PRDX5 in endometrial cancer.

 Competing interests

The authors declare that they have no competing interests.

 Abbreviations PRDX

peroxiredoxin

 hB2MF

human β2-microglobulin

 LVSI

lymphovascular space invasion

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PRDX mRNA expression in endometrial cancer and normal endometrial tissues from benign uterine disease, as determined by semi-quantitative reverse transcription-polymerase chain reaction. All values represent the mean ± standard deviation (bars) of three samples in independent experiments, which were repeated three times with similar results. *P<0.05. PRDX, peroxiredoxin.

Immunohistochemical staining for (A) PRDX3 (magnification, ×200) and (B) PRDX5 (magnification, ×100) in endometrial cancer (arrow) and adjacent normal endometrial glands (star). PRDX, peroxiredoxin.

Survival rates of patients with endometrial cancer according to PRDX3 and PRDX5 expression levels. Kaplan-Meier survival curves showing cumulative survival rates of patients with (A) high or low PRDX3 expression, and (B) high or low PRDX5 expression. Percentages in brackets indicate the survival rate at 5 years. The results indicate that patients with endometrial cancer in the high PRDX3 and PRDX5 expression groups had a decreased 5-year survival rates compared with those in the low PRDX3 and PRDX5 expression groups. However, the associations between survival and PRDX expression were not statistically significant (P=0.2971 and P=0.3818, respectively). PRDX, peroxiredoxin.

Primer sequences for RT-PCR analysis of PRDX.

Gene Primer sequence Product size (bp)
PRDX1 Forward 5′-GGGTATTCTTCGGCAGATCA-3′ 221
Reverse 5′-GCAGCCTGGCACTAAAACAG-3′
PRDX2 Forward 5′-GTGTCCTTCGCCAGATCACT-3′ 154
Reverse 5′-ACGTTGGGCTTAATCGTGTC-3′
PRDX3 Forward 5′-CAAGCAAAATTATTCAGCACCA-3′ 129
Reverse 5′-CCCCTTAAAGTCATCAAGGCT-3′
PRDX4 Forward 5′-GAAATTATCGCTTTTGGCGA-3′ 149
Reverse 5′-AGTGGAATCCTTATTGGCCC-3′
PRDX5 Forward 5′-GTGGTGGCCTGTCTGAGTGT-3′ 150
Reverse 5′-GGACACCAGCGAATCATCTA-3′
PRDX6 Forward 5′-GGATGGGGATAGTGTGATGG-3′   81
Reverse 5′-TTGGTGAAGACTCCTTTCGG-3′
hB2MF Forward 5′-TGACTTTGTCACAGCCCAAG-3′ 265
Reverse 5′-GAGCTACCTGTGGAGCAACC-3′

PRDX, peroxiredoxin; hB2MF, human β2-microglobulin.

Intensity of immunostaining for PRDXs in normal endometrial tissue from cancer and endometrial cancer samples.

Staining intensity, n (%)
PRDX isoform Sample type No. of samples + ++ +++ P-value
1 Normal 24 5 (20.8) 7 (29.2) 12 (50.0) NS
Cancer 42 17 (40.5) 19 (45.2) 6 (14.3)
3 Normal 19 3 (15.8) 10 (52.6) 6 (31.6) 0.0001a
Cancer 42 6 (14.3) 11 (26.2) 25 (59.5)
5 Normal 34 8 (23.5) 7 (20.6) 19 (55.9) 0.0023a
Cancer 42 3 (7.1) 7 (16.7) 32 (76.2)
6 Normal 25 5 (20.0) 16 (64.0) 4 (16.0) NS
Cancer 42 12 (28.6) 22 (52.4) 8 (19.0)

Statistically significant (P<0.05). The total number of normal endometrial samples differs as samples that were not stained were excluded. PRDX, peroxiredoxin; +, weak staining; ++, moderate staining; +++, strong staining; NS, no significance.

Associations between PRDX3/PRDX5 expression and clinicopathological factors in patients with endometrial cancer.

PRDX3 PRDX5
Factor No. of patients Low expression (n=17, 38.5%) High expression (n=25, 61.5%) P-value Low expression (n=10, 23.8%) High expression (n=32, 76.2%) P-value
Age (years)a 42 54.9±11.0 52.1±10.7 0.4107 51.4±7.7 53.8±12.0 0.5371
  Body mass index (kg/m2)a 42 24.3±2.4 25.6±5.1 0.3623 25.4±4.4 24.9±4.3 0.7646
  CA125 (U/ml)a 42 242.6±575.1 36.7±41.9 0.0805 43.8±55.1 143.8±427.3 0.4684
Endometrial cancer typeb 0.9115 0.8002
  Estrogen-dependent 14 5 (35.7) 9 (64.3) 3 (21.4) 11 (78.6)
  Estrogen-independent 28 12 (42.9) 16 (57.1) 7 (25.0) 21 (75.0)
Histological gradeb 0.2372 0.6813
  1 15 4 (26.7) 11 (73.3) 4 (26.7) 11 (73.3)
  2 18 8 (44.4) 10 (55.6) 5 (27.8) 13 (72.2)
  3   8 5 (62.5) 3 (37.5) 1 (12.5) 7 (87.5)
Histological typeb 0.8986 0.5568
  Endometrioid 38 16 (42.1) 22 (57.9) 10 (26.3) 28 (73.7)
  Non-endometrioid   4 1 (25.0) 3 (75.0) 0 (0.0) 4 (100.0)
FIGO stageb 0.1744 0.0399c
  Early 30 10 (33.3) 20 (66.7) 10 (33.3) 20 (66.7)
  Advanced 12 7 (58.3) 5 (41.7) 0 (0.0) 12 (100.0)
Myometrial invasion (%)b 0.7549 0.8363
  <50 24 9 (37.5) 15 (62.5) 6 (25.0) 18 (75.0)
  ≥50 18 8 (44.4) 10 (55.6) 4 (22.2) 14 (77.8)
Lymph node metastasisb 0.2837 0.0838
  No 32 11 (34.4) 21 (65.6) 10 (31.3) 22 (68.8)
  Yes 10 6 (60.0) 4 (40.0) 0 (0.0) 10 (100.0)
LVSIb 0.7397 0.7535
  No 27 11 (40.7) 16 (59.3) 7 (25.9) 20 (74.1)
  Yes 15 6 (40.0) 9 (60.0) 3 (20.0) 12 (80.0)
Recurrenceb 0.9488 0.6611
  No 36 14 (38.9) 22 (61.1) 9 (25.0) 27 (75.0)
  Yes   6 3 (50.0) 3 (50.0) 1 (16.7) 5 (83.3)
Survivalb 0.5546 0.6545
  No   8 2 (25.0) 6 (75.0) 1 (12.5) 7 (87.5)
  Yes 34 15 (44.1) 19 (55.9) 9 (26.5) 25 (73.5)

Data presented as mean ± standard deviation.

Data presented as n (%).

Statistically significant (P<0.05). PRDX, peroxiredoxin; CA125, carbohydrate antigen 125; FIGO, International Federation of Gynecology and Obstetrics; LVSI, lymphovascular space invasion; Early stage, stages I and II; advanced stage, stages III and IV.