A total of 74 patients with HCC admitted to and treated at the Sixth People's Hospital of Qingdao (Qindao, China) between May 2016 and 2018 were selected as research subjects. Inclusion criteria were as follows: HCC diagnosed by pathological examinations; patients with normal function of other major organs; patients with a complete medical record and patients, who had understood the experimental protocol. Exclusion criteria were as follows: Patients combined with other diseases, including chronic diseases other than liver diseases; patients who received treatment within 3 months prior to admission. Liver biopsy was performed on all patients, and tumor tissues as well as adjacent healthy tissues, were collected. Tissues were stored in liquid nitrogen prior to use. The 74 patients included 40 males and 34 females, and age ranged between 36 and 66 years (mean age, 49.1±5.6 years). There were 8 cases of stage I, 18 cases of stage II, 30 cases of stage III and 18 cases of stage IV according to the American Joint Committee on Cancer staging system (14). The 74 patients included 29 HBV-positive cases, 17 HCV-positive cases, 12 both-positive cases and 16 both-negative cases. This study was approved by the ethics committee of Sixth People's Hospital of Qingdao (Qingdao, China). Written informed consent was obtained from all patients.

The present study included two HCC cell lines, SNU-398 and SNU-182. The cell lines and RPMI-1640 medium were purchased from American Type Culture Collection (ATCC). Cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated FBS (ATCC) in an incubator at 37°C with 5% CO₂.

To determine the expression of LINC01638 lncRNA and GLUT1, total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific, Inc.), RT was performed using Applied Biosystems™ High-Capacity cDNA Reverse Transcription kit (Applied Biosystems; Thermo Fisher Scientific, Inc.) and the reverse transcription protocol was 20 min at 55°C and 10 min at 80°C, PCR reaction systems were prepared using a SYBR^® Green Quantitative RT-qPCR kit (Sigma-Aldrich; Merck KGaA). Primers of LINC01638 lncRNA, GLUT1 and β-actin endogenous control were designed and synthesized by Sangon Biotech Co., Ltd. Primer sequences were as follows: 5′-CCTCTGGAATACATCAGCAC-3′ (forward) and 5′-GGTGGAGACTGTAGTGAGCC-3′ (reverse) for LINC01638; 5′-AGGTGATCGAGGAGTTCTAC-3′ (forward) and 5′-TCAAAGGACTTGCCCAGTTT-3′ (reverse) for GLUT1; 5′-GAGACCTTCAACACCCCAGCC-3′ (forward) and 5′-AATGTCACGCACGATTTCCC-3′ (reverse) for β-actin. All PCR reactions were performed on an S1000™ Thermal Cycler (Bio-Rad Laboratories Inc.). The thermocycling conditions were: 95°C for 50 sec, followed by 40 cycles of 95°C for 10 sec and 55°C for 40 sec. Expression of LINC01638 lncRNA and GLUT1 was normalized to β-actin endogenous control using 2^−ΔΔCq method (15). Similar expression results were obtained using 18S rRNA as the endogenous control (data not shown).

LINC01638 lncRNA and GLUT1 expressing vectors (pcDNA3.1) and empty vectors were designed and constructed by Sangon Biotech Co., Ltd. GLUT1 small interfering RNA (siRNA; 5′-CCAAGAGTGTGCTAAAGAATT-3′), LINC01638 siRNA (5′-CATACATACAACTCCAAAAAGT-3′), as well as negative control siRNA (5′-ACAATGAGTCGTAGCATGG-3′) were designed and synthesized by Shanghai GenePharma Co., Ltd. SNU-398 and SNU-182 cell lines were cultured overnight to reach 70–80% confluency and all cell transfections were performed using Lipofectamine^® 2000 (cat. no. 11668-019; Invitrogen; Thermo Fisher Scientific Inc.). All operations were performed in strict accordance with the manufacturer's protocols. Doses of vectors and siRNAs were 10 and 35 nM, respectively. Cells only treated with Lipofectamine^® 2000 were the control cells. Empty vector or negative control siRNA transfection was the negative control. Transfection efficacy was determined by RT-qPCR. In cases of co-transfection, expression vector (10 nM) and siRNA (35 nM) were mixed with cells at the same time.

LINC01638 lncRNA and GLUT1 expression was detected by RT-qPCR at 24 h after transfection. Cells were harvested for glucose uptake assay only when GLUT1/LINC01638 overexpression was >200% and knockdown had reached 50%. Cells of both SNU-398 and SNU-182 cell lines were harvested and washed with PBS buffer. A total of 1×10⁶ cells were subsequently dissolved in Krebs-Ringer-HEPES (KRH) buffer (25 mM HEPES pH 7.4, 1.3 mM KH₂PO₄, 120 mM NaCl, 1.2 mM MgSO₄, 1.3 mM CaCl₂ and 5 mM KCl). A total of 1 µCi of (3H)-2-deoxyglucose was added and cells were incubated at 37°C for 30 min to initiate glucose uptake. Cells were washed with ice-cold KRH buffer to stop glucose uptake. Finally, cells were incubated with lysis buffer (10 mM Tris-HCl pH 8.0, 0.2% SDS) to induce cell lysis, and radioactivity was measured using liquid scintillation spectrometry (LS6500 Liquid Scintillation Counter; Beckman Coulter, Inc.). Disintegrations per minute was used to represent glucose uptake.

LINC01638 lncRNA and GLUT1 expression was detected by RT-qPCR at 24 h after transfection. Cells were harvested for cell proliferation analysis only in cases of LINC01638 lncRNA, and when GLUT1 overexpression rate was >200% and knockdown rate had reached 50%. In brief, cells were harvested, and single cell suspensions were prepared. Cell density was adjusted to 4×10⁴ cells/ml. Cell suspensions were then transferred to a 96-well plate, 0.1 ml per well. Cells were cultivated in an incubator at 37°C with 5% CO₂, followed by the addition of Cell Counting Kit-8 solution (10 µl; Sigma-Aldrich; Merck KGaA) 24, 48, 72 and 96 h later. Cells were subsequently cultivated for an additional 4 h, and optical density values were measured at a wavelength of 450 nm.

LINC01638 lncRNA and GLUT1 expression was detected by RT-qPCR at 24 h following transfection. Cells were harvested for western blot analysis only when GLUT1/LINC01638 overexpression was >200% and knockdown rate had reached 50%. To detect the expression of GLUT1, total protein was extracted using RIPA buffer (Thermo Fisher Scientific, Inc.). Protein concentrations were measured using a bicinchoninic acid assay (Thermo Fisher Scientific, Inc.). Following denaturation, protein samples (30 µg per well) were separated by SDS-PAGE on 12% gels, followed by gel transfer to polyvinylidene difluoride membranes. Membranes were blocked in 5% non-fat milk for 2 h at room temperature, followed by incubation with primary antibodies at 4°C for 18 h: Rabbit anti-human GLUT1 (dilution, 1:1,400; cat. no. ab32551; Abcam) and GAPDH (dilution, 1:1,400; cat. no. ab8245; Abcam). Cells were further incubated with goat anti-rabbit horseradish peroxidase (IgG H&L; cat. no. ab6721; Abcam) for 2 h at 24°C. Enhanced chemiluminescence detection reagent (Sigma-Aldrich; Merck KGaA) was used to develop signals. Signals were normalized using ImageJ 1.8.0 software (National Institutes of Health).

All experiments in this study were performed in triplicate (biological replicates) and mean ± standard deviation was calculated. All statistical analysis was performed using SPSS 19.0 software (IBM Corp., Armonk, NY, USA). Correlations between levels of LINC01638 lncRNA and GLUT1 in tumor tissues and adjacent healthy tissues were performed by Pearson's correlation coefficient. Comparisons of levels of LINC01638 lncRNA and GLUT1 between tumor tissue and adjacent healthy tissues were performed by paired t-test. Comparisons among three groups were performed by one-way ANOVA followed by Tukey's post hoc test. P<0.05 was considered to indicate a statistically significant difference.

Expression levels of LINC01638 lncRNA and GLUT1 mRNA in tumor tissues and adjacent healthy tissues of 74 HCC patients were detected by RT-qPCR. Compared with adjacent healthy tissues, expression levels of LINC01638 lncRNA (Fig. 1A) and GLUT1 mRNA (Fig. 1B) were significantly higher in tumor tissues (P<0.05).

Correlation between expression levels of LINC01638 lncRNA and GLUT1 in tumor tissues and adjacent healthy tissues were performed by Pearson's correlation coefficient. The expression levels of LINC01638 lncRNA and GLUT1 were significantly and positively correlated in tumor tissues (Fig. 2A); however, there was no significant correlation between expression levels of LINC01638 lncRNA and GLUT1 in adjacent healthy tissues (Fig. 2B).

Glucose uptake assay indicated that, compared with the control and the negative control groups, overexpression of LINC01638 lncRNA and GLUT1 led to a significant increase in glucose uptake (Fig. 3A), while LINC01638 lncRNA and GLUT1-knockdown led to a significant inhibition in glucose uptake (Fig. 3B) in SNU-398 and SNU-182 cells (P<0.05).

Compared with the control and the negative control groups, overexpression of LINC01638 lncRNA led to significantly upregulated GLUT1 expression in SNU-398 and SNU-182 cells (Fig. 4A; P<0.05). By contrast, GLUT1 overexpression did not significantly affect LINC01638 lncRNA expression in SNU-398 and SNU-182 cells (Fig. 4B).

Compared with the control and the negative control groups, overexpression of LINC01638 lncRNA and GLUT1 led to increased proliferation, while LINC01638 lncRNA and GLUT1 siRNA silencing led to inhibited proliferation of HCC cells (Fig. 5). In addition, co-transfection experiments revealed that GLUT1 siRNA silencing attenuated the enhancing effects of LINC01638 lncRNA overexpression on cancer cell proliferation (Fig. 5).