ETM Experimental and Therapeutic Medicine 1792-0981 1792-1015 D.A. Spandidos 10.3892/etm.2018.6254 ETM-0-0-6254 Articles Effects of mulberry leaf on experimental hyperlipidemia rats induced by high-fat diet HuangJianbo 1 WangYangpeng 1 YingChao 2 LiuLei 1 LouZhaohuan 3 Department of Basic Theory of Traditional Chinese Medicine, College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China Institute of Materia Medica, College of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China Institute of Materia Medica, College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China Correspondence to: Dr Zhaohuan Lou, Institute of Materia Medica, College of Pharmaceutical Science, Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang, Hangzhou, Zhejiang 310053, P.R. China, E-mail: zhaohuanlou@zcmu.edu.cn 08 2018 06 06 2018 16 2 547 556 02102017 09032018 Copyright: © Huang et al. 2018 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Hypercholesterolemia is a major risk factor for cardiovascular disease. Mulberry leaf (ML) is a Traditional Chinese Medicine used to treat hyperlipidemia in clinical settings. The aim of the present study was to identify the potential effect and possible target of ML in anti-hypercholesterolemia. Male Sprague-Dawley rats were fed with a high-fat diet and treated with ML for 5 weeks. Blood lipid levels, total cholesterol (TC) and total bile acid (TBA) in the liver and feces were measured to assess the effects of ML on hypercholesterolemia. Harris's hematoxylin staining and oil red O staining was applied to observe the pathological change and lipid accumulation in the liver. Immunohistochemical assay was performed to observe the location of expressions of scavenger receptor class B type I and low-density lipoprotein (LDL) receptor (-R), and western blotting was applied to determine the protein expression of ATP-binding cassette transporter G5/G8 (ABCG5/8), nuclear transcription factor peroxisome proliferator-activated receptor-α (PPARα), farnesoid-X receptor (FXR) and cholesterol 7α-hydroxylase 1 (CYP7A1). The results demonstrated that ML treatment reduced serum TC and LDL-cholesterol levels, and liver TC and TBA contents; increased serum HDL-C levels, and fecal TC and TBA contents; and alleviated hepatocyte lipid degeneration. In addition, ML treatment inhibited liver LDL-R, PPARα and FXR protein expression, promoted protein expression of CYP7A1, and maintained the ratio of ABCG5/ABCG8. The findings of the present study provide a positive role of ML on cholesterol clearance via promoting cholesterol and TBA execration via FXR- and CYP7A1-mediated pathways; RCT regulation may be a potential mechanism of ML on anti-hypercholesterolemia.

 hyperlipidemias reverse cholesterol transport bile acid cholesterol 7α-hydroxylase 1 Introduction

It is widely known that hyperlipidemia, particularly hypercholesterolemia, is a notable risk factor for the development of cardiovascular disease (CVD), which is the main cause of mortality worldwide (1), and was recently identified as a causative factor for rotator cuff tear (2). At present, statins, such as lovastatin and atorvastatin, are the most commonly used lipid-lowering drugs, as they efficiently reduce plasma lipids; however, they also present a number of undesirable side effects, such as hepatotoxicity (3), rhabdomyolysis (4) and skeletal muscle injury (5), which have limited their usage. Therefore, it is necessary to identify and develop effective and natural agents that may be valuable in regulating lipid metabolism. In recent years, Traditional Chinese Medicine has attracted greater attention in metabolic syndrome treatments, and has become a common therapy for controlling symptoms in patients with hyperlipidemia (6).

Mulberry leaf (ML), the leaf of Morus alba L., is a Traditional Chinese Medicine that has been used in clinical settings in China for decades. Previous reports have demonstrated that ML extract and powder exhibit anti-type 2 diabetes (7–9), antioxidant (10,11), anti-inflammatory (12), anti-obesity (13,14) and anti-atherosclerosis (15) properties. A number of previous studies have demonstrated that ML powder was effective in reducing serum triglyceride (TG) and low-density lipoprotein (LDL)-cholesterol (C) in patients with mild hyperlipidemia (16,17) and in hyperlipidemia rats (18). In addition, ML may significantly decrease plasma total cholesterol (TC) and increase high-density lipoprotein (HDL)-C (19,20). Valacchi et al (21) have previously reported that a combination of ML and mulberry fruit had a beneficial effect on the regulation of cholesterol transport via modulating the expression of scavenger receptor class B type I (SR-BI) and ATP-binding cassette transporter A1 (ABCA1) in obese mice. As HDL-C is a key factor in reverse cholesterol transport (RCT) (22), which is a mechanism of cholesterol clearance in vivo (23), and SR-BI and ABCA1 are RCT-related proteins, it was hypothesized that ML may regulate the process of RCT to reduce serum cholesterol levels in patients with hyperlipidemia. The aim of the present study was to confirm the effects of ML on serum cholesterol reduction and RCT-related protein expression to identify the potential target.

 Materials and methods <sec> <title>Reagents

ML powder was produced by Shaoxing Royal Tea Village Co., Ltd., (Shaoxing, China) via a process of hot air drying and ball milling technology, and the content of total flavonoids in the powder was 53% which was measured by ultraviolet-visible spectroscopy. ML powder was suspended in pure water according to the dosage with the final concentration as 0.09, 0.06 and 0.03 g/ml prior to intragastric administration. Biochemical assay kits for TC (15110401), TG (15090101), HDL-C (15091401), LDL-C (15100703), aspartate aminotransferase (AST; 15092901), alanine aminotransferase (ALT; 15092401), were purchased from Medicalsystem Biotechnology Co., Ltd (Ningbo, China). ELISA kits for the determination of TG (cat. no. 15090101), TC (cat. no. 20151127) and total bile acid (TBA; 20151128) in the liver and feces were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). High-fat diet (HFD) consisted of standard fodder (82%), lard (10%), cholesterol (1%), cholate (1%) and yolk powder (5%) and was produced by Trophic Animal Feed High-tech Co., Ltd. (Nantong, China).

 Animals and treatments

A total of 48 male Sprague-Dawley (SD) rats (age, 6–8 weeks; weight, 180–200 g) were purchased from the Animal Supply Center of Zhejiang Academy of Medical Science [Hangzhou, China; certificate no. SCXK (Zhe) 2014–0001]. Rats were housed in an environmentally controlled breeding room (temperature, 25±1°C; humidity, 55±5%; 12-h light/dark cycle) for one-week acclimatization prior to experiments. All rats were fed rodent laboratory chow with tap water ad libitum, and were fasted for 12 h prior to experiments with ad libitum access to water. All procedures were performed in strict accordance with the P.R. China Legislation on the Use and Care of Laboratory Animals (24) and with the Animal Management Rules of the Health Ministry of China (25). The present study was approved by the Ethics Committee of Zhejiang Chinese Medical University (Hangzhou, China).

SD rats were divided into six groups (n=8/group) including the normal control (normal), model control (model), atorvastatin (positive control) and three ML-treated (0.9, 0.6, 0.3 g/kg) groups. Animals were given ad libitum access to HFD for 5 weeks, except for the normal group, which were administered the control diet. At the same time, normal and model groups were orally administered distilled water, whereas the atorvastatin and ML groups were administered with 6.0 mg/kg atorvastatin (China Meheco Group Co., Ltd., Beijing, China), or 0.9, 0.6 or 0.3 g/kg ML on a daily basis. At the end of experiment all rats were anesthetized and sacrificed. Livers were harvested for histopathology, immunohistochemical staining and western blot analysis.

 Biochemical analysis and liver TC, TG and TBA assay

Following sacrifice, blood was collected from rat abdominal aortas and centrifuged at 206 × g for 15 min at 4°C to separate the serum. Serum TC, TG, HDL-C, LDL-C, ALT and AST levels were measured using a fully automatic blood biochemistry analyzer (TBA-40FR; Toshiba Medical Systems Corporation, Otawara, Japan) with commercial biochemical kits according to the manufacturer's protocol. Liver homogenate was prepared by grinding 0.5 g liver tissue in 4.5 ml absolute ethyl alcohol followed by centrifugation at 825 × g for 10 min and the supernatant was separated. Levels of TC, TG and TBA in the liver were measured using ELISA kits and a PowerWave 340 microplate reader (BioTek Instruments, Inc., Winooski, VT, USA), according to the manufacturer's protocol.

 Fecal TC and TBA levels determination

Prior to sacrifice, fecal samples were collected from each rat and dried in an oven at ≤80°C. Subsequently, 0.3 g fecal powder was extracted with 3.4 ml absolute ethyl alcohol using an ultrasonic apparatus (KQ-500DE; Kunshan Ultrasonic Instruments Co., Ltd., Kunshan, China) followed by centrifugation at 825 × g for 10 min at room temperature, and the supernatant was separated as the sample for determination. TC and TBA levels were measured using ELISA kits and the PowerWave 340 microplate reader according to the manufacturer's protocol.

 Oil red O/hematoxylin staining

The Oil red O/hematoxylin staining procedure was performed at room temperature as follows: 5-µm frozen liver sections were fixed in 4% paraformaldehyde solution for 10 min, the sections were washed with PBS and stained with Oil red O (WSIG20100803; Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) for 15 min, followed by washing with PBS and staining with Harris's hematoxylin (20151216; Nanjing Jiancheng Bioengineering Institute) staining for 3 min. Lipid droplets were observed under a light microscope (magnification, −200; B5-223IEP; Motic China Group Co., Ltd., Xiamen, China).

 Liver histopathological analysis

Liver tissues were fixed in 10% neutral-buffered formalin for 1 week at 25–27°C, dehydrated in a 70–100% gradient of ethyl alcohol, washed in xylene, embedded in paraffin and cut into 5-µm sections. The liver sections were deparaffinized in xylene, rehydrated in a reverse-gradient series of ethyl alcohol, and stained with hematoxylin for 5 min and eosin for 5 min (H&E; Merck KGaA, Darmstadt, Germany) at room temperature. Pathological changes were observed under a light microscope (magnification, −200), and analyzed with Motic Images Advanced 3.2 software (Motic China Group Co., Ltd.).

 Immunohistochemistry analysis

The location of SR-BI and low-density lipoprotein LDL-receptor (LDL-R) in liver tissue was evaluated via immunohistochemistry. In brief, liver specimens fixed in 10% formalin at room temperature for 1 week, were embedded in paraffin wax, cut into 5-µm-thick sections, deparaffinized in xylene and rehydrated in a reverse-gradient series of ethyl alcohol. Following treatment with 3% hydrogen peroxide for 15 min at room temperature to block endogenous peroxidase activity, the sections were incubated with primary antibodies against SR-BI (1:100; ab52629; Abcam, Cambridge, UK) and LDL-R (1:100; sc-11824; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) for 12 h at 4°C then washed with PBS. Biotinylated goat anti-polyvalent secondary antibodies [1:200; Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit; ab64264; Abcam] were added and incubated for 20 min at room temperature. The specimens were subsequently incubated with streptavidin peroxidase for 10 min at room temperature and DAB (1:50) was applied to visualize the labeling. The positive area was identified with brown staining under the B5-223IEP light microscope (magnification, −400).

 Western blot analysis

Liver tissues (~100 mg) were homogenized with liquid nitrogen, lysed with radioimmunoprecipitation buffer (P0013B; Beyotime Institute of Biotechnology, Haimen, China) and protease/phosphatase inhibitors for 30 min on ice, and centrifuged at 18,246 × g for 15 min at 4°C. Total proteins were quantified using the BCA method with a protein quantitation kit (P0012; Beyotime Institute of Biotechnology). Protein samples (50 µg/lane) were separated by 10% SDS-PAGE and transferred onto polyvinylidene fluoride membranes. The membranes were blocked with 5% skimmed milk in Tris-buffered saline containing 0.05% Tween-20 (TBST) for 120 min at room temperature. Following overnight incubation at 4°C with the following primary antibodies: Peroxisome proliferator-activated receptor-α (PPARα; 1:1,000; 15540-1-AP, ProteinTech Group, Inc., Chicago, IL, USA), SR-BI (1:1,000; ab52629, Abcam), LDL-R (1:1,000; sc-11824, Santa Cruz Biotechnology, Inc.), ATP-binding cassette transporter (ABC)G5 (1:1,000; bs-5013R; BIOSS, Beijing, China), ABCG8 (1:1,000; bs-10149R; BIOSS), farnesoid-X receptor (FXR; 1:1,000; NR1H4, YN2161; ImmunoWay Biotechnology Company, Plano, TX, USA) cholesterol 7α-hydroxylase 1 (CYP7A1; 1:1,000; sc-14426, Santa Cruz Biotechnology, Inc.), and GAPDH (1:1,000; B661204-0001, Sangon Biotech Co., Ltd., Shanghai, China); the membranes were washed three times with TBST (10 min each time) and subsequently incubated with HRP-conjugated rabbit anti-mouse immunoglobulin G secondary antibodies (cat. no. 58802; 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA) for 1 h at room temperature. Visualization was performed with an enhanced chemiluminescence detection reagent (GE Healthcare, Chicago, IL, USA), and GAPDH was used as an internal control. Expression levels were quantified using ImageJ 1.46r image analysis software (National Institutes of Health, Bethesda, MD, USA).

 Statistical analysis

Data are presented as the mean ± standard error of the mean. One-way analysis of variance with Fisher's least-significant difference post hoc analysis for multiple comparisons was applied to compare differences among multiple groups. P<0.05 was considered to indicate a statistically significant difference.

 Results <sec> <title>Effects of ML on serum lipid levels and fecal TC and TBA levels

As presented in Fig. 1, serum TC (P<0.01), TG (P<0.05) and LDL-C (P<0.01) levels, and fecal TC (P<0.05) and TBA (P<0.01) levels in the model group were significantly increased, compared with the normal group, whereas serum HDL-C levels were significantly decreased (P<0.01). Compared with the model group, atorvastatin significantly lowered serum TC and LDL-C levels (P<0.01), however no other significant differences were observed. ML treatment reduced TC (0.6 and 0.3 g/kg; P<0.05), TG (0.6 g/kg, P<0.01; 0.3 g/kg, P<0.05) and LDL-C (all, P<0.01) concentrations, and 0.6 g/kg ML significantly increased HDL-C levels (P<0.05). Conversely, in ML treatment groups, fecal TC (0.6 g/kg, P<0.01; 0.3 g/kg, P<0.05) and TBA (0.6 and 0.3 g/kg, P<0.01) levels were significantly increased, compared with the model group. These findings suggest that the serum cholesterol reduction of ML may be associated with the excretion of TC and TBA.

 Effects of ML on liver pathological changes and hepatic lipidosis

Serum AST activity was significantly increased in model rats compared with the normal group (P<0.01; Fig. 2A), and ALT activity exhibited a similar, marked trend (Fig. 2B). Representative images of liver histology demonstrated that normal rats presented normal liver histology, as hepatocytes were observed with a common radial array encircling the central veins and no hepatocyte lipid degeneration was observed (Fig. 2C-a). In model rats, the lobular structures of hepatocytes were disrupted and inflammatory cell infiltration was observed (Fig. 2C-b). Atorvastatin significantly reduced serum AST and ALT activity compared with the model group (Fig. 2A and B), and notably improved hepatocyte lipid degeneration (Fig. 2C-c). ML reduced serum AST activity in a dose-dependent manner (0.6 g/kg, P<0.05; 0.3 g/kg, P<0.01; Fig. 2A), and induced a marked decrease in ALT activity (Fig. 2B), compared with the model group. ML also markedly reduced neutrophil infiltration (Fig. 2C-d-f).

Liver TC (Fig. 3A) and TBA (Fig. 3B) levels in the model group were significantly increased (P<0.01 and <0.05, respectively) compared with normal rats, and liver TG exhibited a similar, marked increase (Fig. 3A). Lipid droplets were visible in the hepatic plates, and oil red O staining revealed a pale pink staining in the normal rat hepatic tissue (Fig. 3C-a), while the model rats exhibited severe hepatic steatosis (Fig. 3C-b). ML significantly decreased liver TC (0.9 and 0.6 g/kg, P<0.01; 0.3 g/kg, P<0.05; Fig. 3A) and TBA (0.9 g/kg, P<0.05; Fig. 3B) levels, and the color and density of oil red O staining of the liver was weakened, indicating that Atorastatin and ML markedly alleviated hepatocyte lipid degeneration (Fig. 3C-c-f).

 Effects of ML on expressions of cholesterol absorption-related proteins in liver

According to the effects of ML on serum and liver lipid level and fecal TC and TBA level, two of the ML treated groups (0.6 and 0.3 g/kg) were chosen to observe the expression of cholesterol absorption and excretion-related proteins in the liver.

LDL-R and SR-BI are associated with the transportation of cholesterol from peripheral blood to the liver (26,27). As demonstrated in representative images of immunohistochemistry, LDL-R protein was predominantly located at the hepatic portal vein area (Fig. 4A). In the normal, Atorastatin and ML groups, LDL-R was primarily located at the hepatocytes close to the vein (Fig. 4A-a, c and d) while in the model group it was observed in a wider range (Fig. 4A-b). SR-BI was predominantly located at the hepatocyte membrane and no notable differences were observed between the groups (Fig. 4B-a-d). Western blotting revealed that the SR-BI protein expression in model rats was significantly decreased compared with normal rats (P<0.01), whereas LDL-R expression was significantly increased (P<0.01; Fig. 5A and B). Atorastatin and ML significantly reduced SR-BI (0.6 g/kg, P<0.01) and LDL-R (both, P<0.01) levels compared with the model group (Fig. 5A and B).

 Effects of ML on expressions of cholesterol excretion related proteins in liver

ABCG5, ABCG8, FXR, PPARα and CYP7A1 are associated with the conversion of cholesterol into TBA in the liver, and the excretion of TBA in feces. Compared with the normal group, a decrease was observed in ABCG5 and ABCG8 (P<0.01) protein expression in the model group (Fig. 5A and C), and the ratio of ABCG5/ABCG8 in the model group was significantly increased, compared with that of the normal group (P<0.01; Fig. 5D). This indicated that the balance of ABCG5 and ABCG8 was changed. ABCG5 and ABCG8 protein expressions were significantly decreased in Atorastatin and ML-treated rats compared with the model group (both 0.6 g/kg, P<0.05; 0.3 g/kg, P<0.01), however the ratio of ABCG5/ABCG8 was significantly lower compared with that of the model rats (both, P<0.01), indicating that ML was able to maintain the balance of ABCG5 and ABCG8 expression in the liver.

Compared with normal rats, expression of PPARα protein was increased significantly in the model group (P<0.05), whereas FXR and CYP7A1 were significantly decreased (both, P<0.01; Fig. 5E). ML at 0.6 and 0.3 g/kg significantly decreased the expression of PPARα and FXR protein in liver, compared with model rats (all, P<0.01), whereas 0.3 g/kg ML significantly increased the protein expression of CYP7A1 (P<0.01; Fig. 5A and E).

 Discussion

Hypercholesterolemia accompanied by high serum LDL-C and low HDL-C is a main factor for the development of atherosclerotic disease (28,29). Excess diet-derived cholesterol is the primary cause for hypercholesterolemia (30). In the present study, rats fed with HFD for 5 weeks exhibited significantly increased serum TC and LDL-C levels. ML treatment significantly reduced the accumulation of TC in plasma and the liver, and alleviated hepatocyte lipid deposition in high-fat diet-fed rats. H&E and oil red O staining images presented lipid accumulation in the livers of rats fed an HFD, and a marked increase of liver TG levels was observed in the HFD group compared with the normal group. There are two potential reasons for these findings: i) Intra-group variance induced a non-significant difference between HFD group and normal group; and ii) HFD, which increased the serum TC level, induced TC accumulation in the liver.

RCT is a process that encompasses the transport of excess cholesterol from peripheral tissues to the liver for biliary and fecal excretion (31). It is an effective method for cholesterol homeostasis in vivo (23) and a protective mechanism to contract atherosclerotic injury (32). Various cholesterol transporters, including SR-BI, ABCG5, ABCG8 and CYP7A1 are associated with RCT, and increase cholesterol transportation and conversion (33). Therefore, medicines which regulate expression of these proteins are potential candidates for hypercholesterolemia treatment.

LDL-R has a role in regulating plasma cholesterol level and cholesterol homeostasis by limiting hepatic uptake of circulating cholesterol (29). However, contrary to recent findings (32), the present study demonstrated that the expression of LDL-R in liver cells was significantly increased in rats with hyperlipidemia, whereas it was decreased in ML-treated rats. The LDL-R pathway is complex (34,35), and with the previous identification of the LDL-R-proprotein convertase subtilisin/kexin type 9 (PCSK9)-LDL axis in the LDL-R pathway for cholesterol homeostasis, Kosenko et al (36) demonstrated that plasma LDL particles reduce PCSK9-mediated LDL-R degradation in a dose-dependent manner by binding to PCSK9. This finding suggested that decreased plasma LDL may induce a lower expression of LDLR in hepatocytes. However, whether there is an association among serum LDL, hepatic LDL-R, PCSK9 and ML required further study.

SR-BI has a crucial role in cholesterol homeostasis and hepatic SR-BI mediates the final step in RCT via the uptake of HDL-C for routing to the bile (27,37). The suppression of hepatic SR-BI expression impairs HDL-mediated RCT and induces hypercholesterolemia (38,39). In the present study, it was demonstrated that SR-BI protein expression in the liver exhibited a decrease in model rats and ML treatment did not block this decline, which suggested that the SR-BI signal pathway may not be the target of ML in mediating the transportation of cholesterol.

ABCG5 and ABCG8 are expressed in the liver and intestine. They typically form a heterodimer in the endoplasmic reticulum, which pumps neutral sterols back into the gut lumen to prevent the accumulation of other sterols (40,41) and partially contributes to the trans-intestinal cholesterol efflux pathway (42). ABCG5/ABCG8 promotes SR-BI-transported cholesterol excretion in the bile, and eventually in feces, to prohibit the development of hypercholesterolemia, and inactivation of either protein induces sitosterolemia (43). In the current study, TC and TBA levels in the liver and feces were significantly increased in model rats compared with the normal group; in ML-treated rats, liver TC and TBA levels were significantly decreased while fecal TC and TBA levels were significantly increased compared with the model rats, indicating that in addition to the compensatory excretion of cholesterol in rats with HFD intake, ML promoted the clearance of cholesterol by TBA from feces in rats with hypercholesterolemia. Western blotting results demonstrated that ML could maintain the balance of ABCG5 and ABCG8 protein expression in the liver. Together, these findings suggested that maintaining the stabilization and activity of ABCG5/ABCG8 protein may be a potential mechanism for ML to lower serum cholesterol.

The conversion of cholesterol into BA via intestinal and biliary lumen for fecal excretion is the final step to remove cholesterol from the body. CYP7A1 is a rate-limiting enzyme in cholesterol conversion to BA. Promoting CYP7A1 expression accelerates the cholesterol conversion into BA and the excretion in feces (44,45). PPARα serves a role in the clearance of circulating cholesterol via upregulating CYP7A1 expression in hepatocytes (45). Farnesoid-X receptor (FXR) is an intracellular BA receptor, which can be activated by BA, and has a pivotal role for BA and lipid homeostasis; overexpression of FXR decreases BA pools and reduces fecal BA excretion (46) due to the suppression of hepatic CYP7A1 (47). In the present study, 5-week HFD intake increased PPARα protein expression, and decreased FXR and CYP7A1 expression in liver, whereas increased levels of TC and TBA in feces were observed. ML treatment markedly inhibited PPARα and FXR protein expression, upregulated CYP7A1 expression, increased fecal TBA levels, and simultaneously reduced cholesterol in the liver and plasma. Together, these findings suggest that ML may promote the conversion of cholesterol into BA and excretion to accelerate the clearance of circulating cholesterol. The present study focused primarily on extrinsic cholesterol excretion from hepatocytes, and the effect of ML on cholesterol synthesis will be considered in future studies. Meanwhile, changes in liver and fecal TBA levels provide rationale that regulating enterohepatic circulation of BA may be a potential mechanism of ML on reduced serum TC levels.

As detailed above, ML exhibited a beneficial effect on anti hyperlipidemia, but no obvious dose-effect association was observed on serum TG and HDL-C levels, and fecal TC and TBA levels. Previous studies have demonstrated that ML contains 1-dexoynokifimycin (48), polyphenols and flavonoids (49), which are active components on blood lipid regulation, anti-atherosclerosis and anti-oxidation (50). In these studies, the test sample was administered an extract of ML in which the active components were enriched to induce a more notable effect. ML is edible and may be used to make tea, which may be a convenient method to prevent hyperlipidemia in the future. In the present study, ML powder was produced via a process of hot air drying and ball milling technology as the test sample. As this sample contained all the components of ML, it was not possible to identify an exact dose-effect relationship, but the present results have identified an effective dosage for intake. However, if ML is to be considered as a candidate medicine for hypercholesterolemia treatment, it is essential that the chemical material basis and the dose-effect relationship are elucidated.

The findings of the present study suggest a positive role of ML on cholesterol clearance by promoting cholesterol and TBA execration via FXR- and CYP7A1-mediated pathways, and that RCT regulation may be a potential mechanism of ML on anti hypercholesterolemia.

 Acknowledgements

The authors would like to thank Dr Gao Jianli of Zhejiang Chinese Medical University (Hangzhou, China) for their assistance and revision of the manuscript.

 Funding

The present study was supported by Zhejiang Provincial Natural Science Foundation of China (grant no. LY15H280007), National Natural Science Foundation of China (grant no. 81503328), and The Zhejiang Provincial Key Laboratory Project (grant no. 2012E10002).

 Availability of data and materials

The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.

 Authors' contributions

JH designed and organized the study. YW and LL performed the animal experiments. CY performed the western blot analysis. ZL performed the data analysis and wrote the manuscript.

 Ethics approval and consent to participate

The present study was approved by the Ethics Committee of Zhejiang Chinese Medical University (Hangzhou, China).

 Consent for publication

Not applicable.

 Competing interests

The authors declare that they have no competing interests.

 References Navar-BogganAMPetersonEDD'AgostinoRBSrNeelyBSnidermanADPencinaMJHyperlipidemia in early adulthood increases long-term risk of coronary heart diseaseCirculation131451458201510.1161/CIRCULATIONAHA.114.01247725623155 GarciaGHLiuJNWongACordascoFDinesDMDinesJSGulottaLVWarrenRHyperlipidemia increases the risk of retear after arthroscopic rotator cuff repairJ Shoulder Elbow Surg2620862090201710.1016/j.jse.2017.05.00928735844 BjornssonEJacobsenEIKalaitzakisEHepatotoxicity associated with statins: Reports of idiosyncratic liver injury post-marketingJ Hepatol56374380201210.1016/j.jhep.2011.07.02321889469 GrahamDJStaffaJAShatinDAndradeSESchechSDLa GrenadeLGurwitzJHChanKAGoodmanMJPlattRIncidence of hospitalized rhabdomyolysis in patients treated with lipid-lowering drugsJAMA29225852590200410.1001/jama.292.21.258515572716 SakamotoKHondaTYokoyaSWaguriSKimuraJRab-small GTPases are involved in fluvastatin and pravastatin-induced vacuolation in rat skeletal myofibersFASEB J2140874094200710.1096/fj.07-8713com17634390 ChuSMShihWTYangYHChenPCChuYHUse of traditional Chinese medicine in patients with hyperlipidemia: A population-based study in TaiwanJ Ethnopharmacol168129135201510.1016/j.jep.2015.03.04725828254 CaiSSunWFanYGuoXXuGXuTHouYZhaoBFengXLiuTEffect of mulberry leaf (Folium Mori) on insulin resistance via IRS-1/PI3K/Glut-4 signalling pathway in type 2 diabetes mellitus ratsPharm Biol5426852691201610.1080/13880209.2016.117877927158744 SalemiZBarzin TondSFallahSShojaiiASeifiMThe effect of Morus alba leaves extract and powder on resistin levels and liver transaminase enzymes activities in diabetesCell Mol Biol (Noisy-le-Grand)62112118201627262814 ZhangYRenCLuGCuiWMuZGaoHWangYPurification, characterization and anti-diabetic activity of a polysaccharide from mulberry leafRegul Toxicol Pharmacol70687695201410.1016/j.yrtph.2014.10.00625455227 KhanMARahmanAAIslamSKhandokharPParvinSIslamMBHossainMRashidMSadikGNasrinSA comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L(Moraceae). BMC Res Notes624201310.1186/1756-0500-6-24 LeeYJHsuJDLinWLKaoSHWangCJUpregulation of caveolin-1 by mulberry leaf extract and its major components, chlorogenic acid derivatives, attenuates alcoholic steatohepatitis via inhibition of oxidative stressFood Funct8397405201710.1039/C6FO01539E28074952 JeongJWLeeHHLeeKWKimKYKimSGHongSHKimGYParkCKimHKChoiYWChoiYHMori folium inhibits interleukin-1β-induced expression of matrix metalloproteinases and inflammatory mediators by suppressing the activation of NF-κB and p38 MAPK in SW1353 human chondrocytesInt J Mol Med37452460201610.3892/ijmm.2015.244326707272 AnnJYEoHLimYMulberry leaves (Morus alba L.) ameliorate obesity-induced hepatic lipogenesis, fibrosis, and oxidative stress in high-fat diet-fed miceGenes Nutr1046201510.1007/s12263-015-0495-x26463593 SugimotoMAraiHTamuraYMurayamaTKhaengkhanPNishioTOnoKAriyasuHAkamizuTUedaYMulberry leaf ameliorates the expression profile of adipocytokines by inhibiting oxidative stress in white adipose tissue in db/db miceAtherosclerosis204388394200910.1016/j.atherosclerosis.2008.10.02119070857 EnkhmaaBShiwakuKKatsubeTKitajimaKAnuuradEYamasakiMYamaneYMulberry (Morus alba L.) leaves and their major flavonol quercetin 3-(6-malonylglucoside) attenuate atherosclerotic lesion development in LDL receptor-deficient miceJ Nutr135729734200510.1093/jn/135.4.72915795425 AramwitPPetcharatKSupasyndhOEfficacy of mulberry leaf tablets in patients with mild dyslipidemiaPhytother Res25365369201120687135 AramwitPSupasyndhOSiritienthongTBangNMulberry leaf reduces oxidation and C-reactive protein level in patients with mild dyslipidemiaBiomed Res Int2013787981201310.1155/2013/78798123484158 KobayashiYMiyazawaMKameiAAbeKKojimaTAmeliorative effects of mulberry (Morus alba L.) leaves on hyperlipidemia in rats fed a high-fat diet: Induction of fatty acid oxidation, inhibition of lipogenesis, and suppression of oxidative stressBiosci Biotechnol Biochem7423852395201010.1271/bbb.10039221150120 LouZZhangGSuJXiaBYuJYanMEffect of green tea and mulberry leaf powder on serum lipid profile in rats with hyperlipidemiaZhongchengyao38159415972016(In Chinese) TrimarcoVIzzoRStabileERozzaFSantoroMManziMVSerinoFSchiattarellaGGEspositoGTrimarcoBEffects of a new combination of nutraceuticals with Morus alba on lipid profile, insulin sensitivity and endotelial function in dyslipidemic subjectsA cross-over, randomized, double-blind trial. High Blood Press Cardiovasc Prev22149154201510.1007/s40292-015-0087-2 ValacchiGBelmonteGMiraccoCEoHLimYEffect of combined mulberry leaf and fruit extract on liver and skin cholesterol transporters in high fat diet-induced obese miceNutr Res Pract82026201410.4162/nrp.2014.8.1.2024611101 RiwantoMLandmesserUHigh density lipoproteins and endothelial functions: Mechanistic insights and alterations in cardiovascular diseaseJ Lipid Res5432273243201310.1194/jlr.R03776223873269 van der VeldeAEVrinsCLvan den OeverKSeemannIOude ElferinkRPvan EckMKuipersFGroenAKRegulation of direct transintestinal cholesterol excretion in miceAm J Physiol Gastrointest Liver Physiol295G203G208200810.1152/ajpgi.90231.200818511744 The State Science and Technology Commission of ChinaRegulations on the management of laboratory animalsShiyong Qiguan Yizhi Zazhi466672016(In Chinese) Ministry of Science and Technology of the People's Republic of China2018Methods for managing experimental animal licenses (trial)[online] Available at:http://www.most.gov.cn/fggw/zfwj/zfwj2001/zf01yw/zf01kjjh/200312/t20031209_31332.htmJuly302015 BrownMSGoldsteinJLA receptor-mediated pathway for cholesterol homeostasisScience2323447198610.1126/science.35133113513311 HubyTDoucetCDachetCOuzilleauBUedaYAfzalVRubinEChapmanMJLesnikPKnockdown expression and hepatic deficiency reveal an atheroprotective role for SR-BI in liver and peripheral tissuesJ Clin Invest11627672776200610.1172/JCI2689316964311 BraamskampMJHuttenBAWiegmanAEarly initiation of statin treatment in children with familial hypercholesterolaemiaCurr Opin Lipidol26236239201510.1097/MOL.000000000000017725943840 YakushijiEAyaoriMNishidaTShiotaniKTakiguchiSNakayaKUto-KondoHOguraMSasakiMYogoMProbucol-oxidized products, spiroquinone and diphenoquinone, promote reverse cholesterol transport in miceArterioscler Thromb Vasc Biol36591597201610.1161/ATVBAHA.115.30637626848156 WangDQRegulation of intestinal cholesterol absorptionAnnu Rev Physiol69221248200710.1146/annurev.physiol.69.031905.16072517002594 OrsoniAVillardEFBruckertERobillardPCarrieABonnefont-RousselotDChapmanMJDallinga-ThieGMLe GoffWGuerinMImpact of LDL apheresis on atheroprotective reverse cholesterol transport pathway in familial hypercholesterolemiaJ Lipid Res53767775201210.1194/jlr.M02414122338009 ZhangYSiYZhaiLGuoSZhaoJSangHPangXZhangXChenAQinSCelastrus orbiculatus thunbReduces lipid accumulation by promoting reverse cholesterol transport in hyperlipidemic mice. Lipids516776922016 ZhuRGSunYDHouYTFanJGChenGLiTPPectin penta-oligogalacturonide reduces cholesterol accumulation by promoting bile acid biosynthesis and excretion in high-cholesterol-fed miceChem Biol Interact272153159201710.1016/j.cbi.2017.05.01828549616 MaxwellKNFisherEABreslowJLOverexpression of PCSK9 accelerates the degradation of the LDLR in a post-endoplasmic reticulum compartmentProc Natl Acad Sci USA10220692074200510.1073/pnas.040973610215677715 ZelcerNHongCBoyadjianRTontonozPLXR regulates cholesterol uptake through Idol-dependent ubiquitination of the LDL receptorScience325100104200910.1126/science.116897419520913 KosenkoTGolderMLeblondGWengWLagaceTALow density lipoprotein binds to proprotein convertase subtilisin/kexin type-9 (PCSK9) in human plasma and inhibits PCSK9-mediated low density lipoprotein receptor degradationJ Biol Chem28882798288201310.1074/jbc.M112.42137023400816 JiYWangNRamakrishnanRSehayekEHuszarDBreslowJLTallARHepatic scavenger receptor BI promotes rapid clearance of high density lipoprotein free cholesterol and its transport into bileJ Biol Chem2743339833402199910.1074/jbc.274.47.3339810559220 BraunAZhangSMiettinenHEEbrahimSHolmTMVasileEPostMJYoergerDMPicardMHKriegerJLProbucol prevents early coronary heart disease and death in the high-density lipoprotein receptor SR-BI/apolipoprotein E double knockout mouseProc Natl Acad Sci USA10072837288200310.1073/pnas.123772510012771386 ChulskySPalandNLazarovichAFuhrmanBUrokinase-type plasminogen activator (uPA) decreases hepatic SR-BI expression and impairs HDL-mediated reverse cholesterol transportAtherosclerosis2331118201410.1016/j.atherosclerosis.2013.11.07024529115 BergeKETianHGrafGAYuLGrishinNVSchultzJKwiterovichPShanBBarnesRHobbsHHAccumulation of dietary cholesterol in sitosterolemia caused by mutations in adjacent ABC transportersScience29017711775200010.1126/science.290.5497.177111099417 WangJMitscheMALutjohannDCohenJCXieXSHobbsHHRelative roles of ABCG5/ABCG8 in liver and intestineJ Lipid Res56319330201510.1194/jlr.M05454425378657 van der VeenJNvan DijkTHVrinsCLvan MeerHHavingaRBijsterveldKTietgeUJGroenAKKuipersFActivation of the liver X receptor stimulates trans-intestinal excretion of plasma cholesterolJ Biol Chem2841921119219200910.1074/jbc.M109.01486019416968 DikkersAFreak de BoerJAnnemaWGroenAKTietgeUJScavenger receptor BI and ABCG5/G8 differentially impact biliary sterol secretion and reverse cholesterol transport in miceHepatology58293303201310.1002/hep.2631623401258 CaoYBeiWHuYCaoLHuangLWangLLuoDChenYYaoXHeWHypocholesterolemia of Rhizoma Coptidis alkaloids is related to the bile acid by up-regulated CYP7A1 in hyperlipidemic ratsPhytomedicine19686692201210.1016/j.phymed.2012.03.01122554715 LiTMatozelMBoehmeSKongBNilssonLMGuoGEllisEChiangJYOverexpression of cholesterol 7alpha-hydroxylase promotes hepatic bile acid synthesis and secretion and maintains cholesterol homeostasisHepatology539961006201110.1002/hep.2410721319191 SinalCJTohkinMMiyataMWardJMLambertGGonzalezFJTargeted disruption of the nuclear receptor FXR/BAR impairs bile acid and lipid homeostasisCell102731744200010.1016/S0092-8674(00)00062-311030617 CaiSYHeHNguyenTMennoneABoyerJLRetinoic acid represses CYP7A1 expression in human hepatocytes and HepG2 cells by FXR/RXR-dependent and independent mechanismsJ Lipid Res5122652274201010.1194/jlr.M00554620338915 KojimaYKimuraTNakagawaKAsaiAHasumiKOikawaSMiyazawaTEffects of mulberry leaf extract rich in 1-deoxynojirimycin on blood lipid profiles in humansJ Clin Biochem Nutr47155161201010.3164/jcbn.10-5320838571 ChanKCYangMYLinMCLeeYJChangWCWangCJMulberry leaf extract inhibits the development of atherosclerosis in cholesterol-fed rabbits and in cultured aortic vascular smooth muscle cellsJ Agric Food Chem6127802788201310.1021/jf305328d23428158 HeXFangJRuanYWangXSunYWuNZhaoZChangYNingNGuoHHuangLStructures, bioactivities and future prospective of polysaccharides from Morus alba (white mulberry): A reviewFood Chem245899910201810.1016/j.foodchem.2017.11.08429287458

Effects of ML on serum lipid levels and fecal TC and TBA levels in high-fat diet-fed rats. (A) Serum TC levels; (B) serum TG levels; (C) serum LDL-C levels; (D) serum HDL-C levels; (E) fecal TC levels; (F) fecal TBA levels. Data are presented as the mean ± standard error of the mean (n=8). ∆P<0.05, ∆∆P<0.01, vs. normal; *P<0.05, **P<0.01 vs. model. ML, mulberry leaf; TC, total cholesterol; TBA, total bile acid; TG, triglyceride; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-density lipoprotein cholesterol.

Effects of ML on liver functions in high-fat diet-fed rats. (A) Serum AST activities; (B) serum ALT activities. (C) Representative images of liver histology. The black arrow indicates neutrophil infiltration or hepatocyte structure injury (hematoxylin-eosin staining; magnification, −200). (a) Normal; (b) model; (c) atorvastatin; (d) 0.9 g/kg ML; (e) 0.6 g/kg ML; (f) 0.3 g/kg ML. Data are presented as the mean ± standard error of the mean (n=8). ∆∆P<0.01, vs. normal; *P<0.05, **P<0.01 vs. model. ML, mulberry leaf; AST, aspartate aminotransferase; ALT, alanine aminotransferase.

Effects of ML on liver fatty degeneration in high-fat diet-fed rats. (A) Liver TC and TG levels; (B) liver TBA levels. (C) Representative images of hepatocyte lipid droplet (oil red O staining; original magnification, −200) (a) Normal; (b) model; (c) atorvastatin; (d) 0.9 g/kg ML; (e) 0.6 g/kg ML; (f) 0.3 g/kg ML. Data are presented as the mean ± standard error of the mean (n=8). ∆P<0.05, ∆∆P<0.01, vs. normal; *P<0.05, **P<0.01 vs. model. ML, mulberry leaf; TC, total cholesterol; TG, triglyceride; TBA, total bile acid.

Localization of SR-BI and LDL-R protein expression in livers of high-fat diet-fed rats. (A) Representative immunohistochemistry images of SR-BI in liver (HRP/DAB staining; original magnification, −200). (B) Representative immunohistochemistry images of LDL-R in liver (HRP/DAB staining; original magnification, −200). (a) Normal; (b) model; (c) atorvastatin; (d) ML. SR-BI, scavenger receptor class B type I; LDL-R, low-density lipoprotein receptor; HRP, horseradish peroxidase; DAB, 3,3′-diaminobenzidine.

Expressions of cholesterol absorption, conversion and bile acid excretion-associated proteins in the livers of HFD-fed rats. (A) Western blotting images of SR-BI and LDL-R, ABCG5, ABCG8, PPARα, FXR and CYP7A1 protein expression. (B) Relative expression of SR-BI and LDL-R protein. (C) Relative expression of ABCG5 and ABCG8 protein. (D) Ratio of ABCG5/ABDG8 expression. (E) Relative expression of PPARα, FXR and CYP7A1 protein. Data are presented as the mean ± standard error of the mean (n=8). ∆P<0.05, ∆∆P<0.01, vs. normal; *P<0.05, **P<0.01 vs. model. SR-BI, scavenger receptor class B type I; LDL-R, low-density lipoprotein receptor; ABCG5, ATP-binding cassette transporter G5; ABCG8, ATP-binding cassette transporter G8; PPARα, peroxisome proliferator-activated receptor-α; FXR, farnesoid-X receptor; CYP7A1, cholesterol 7α-hydroxylase 1; HFD, high-fat diet; ML, mulberry leaf.