Human glioma cell lines (U251, U343, U87, SF126 and SF76) were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Gibco) at 37°C in a humidified atmosphere of 5% CO₂.

This study was approved by the Ethics Committee of the Third Xiangya Hospital of Central South University. All patients provided written informed consent in compliance with the code of ethics of the World Medical Association (Declaration of Helsinki). Human glioma samples and adjacent normal tissue samples were collected from 20 glioma patients who underwent surgery at the Neurosurgery Department of the Third Xiangya Hospital of Central South University (Changsha, Hunan, China). At the time of diagnosis, 10 patients had early stage (I, II) whereas the other 10 patients had stage III and IV glioma. The tissue samples were obtained from surgery and immediately frozen in liquid nitrogen.

Total RNAs were isolated from cells and tissues using TRIzol (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. Reverse transcription was performed using a RevertAid™ First Strand cDNA Synthesis kit (Fermentas, Vilnius, Lithuania). Real-time PCR reaction was carried out in a 7900 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) using a SYBR-Green PCR kit (Applied Biosystems). The relative mRNA expression level was calculated by the comparative Ct method.

Tissues and cells were lysed by ice-cold protein extraction buffer (150 mM Tris-HCl, pH 7.4, 120 mM NaCl, 2 mM EDTA, 50 mM sodium fluoride, 0.2% SDS, 1% Nonidet P-40, 100 mM sodium vanadate and 1 mM phenylmethylsulfonyl fluoride). Proteins were quantitated using the BCA kit (Beyotime, Shanghai, China). The protein samples were separated in 10% SDS-PAGE and transferred onto a nitrocellulose membrane (Millipore, Billerica, MA, USA). The membranes were blocked overnight in 5% non-fat milk and then incubated with the primary antibody [IQGAP1 and β-catenin antibodies (Abcam, Cambridge, MA, USA); p-β-catenin and cyclin D1 antibodies (Cell Signaling Technology, Inc., Beverly, MA, USA); GAPDH antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA)] for 1 h at room temperature. After washing with PBS, the membranes were incubated with the secondary antibody [HRP-conjugated goat anti-mouse IgG and HRP-conjugated goat anti-rabbit IgG (Santa Cruz Biotechnology, Inc.)] for 1 h at room temperature. Detection was performed using a chemiluminescence-based detection system (ECL western blotting kit; Pierce Biotechnology, Inc., Rockford, IL, USA).

Cells were washed with PBS and fixed in 4% paraformaldehyde solution for 1 h. After permeabilization with 0.2% Triton X-100 at 4°C for 1 h, cells were blocked with goat serum and then incubated with the primary antibody (β-catenin antibody) for 1 h at 37°C. Subsequently, the secondary antibody [Alexa Fluor 488-labeled anti-rabbit IgG (Cell Signaling Technology Inc.)] was added, and the cells were incubated at 37°C for 1 h. DAPI (Santa Cruz Biotechnology, Inc.) was used to label the nucleus, and the cells were visualized by fluorescence microscopy (Nikon, Tokyo, Japan).

The miR-124a mimic and siRNAs were synthesized by Biomics Biotechnologies Inc. (Nantong, Jiangsu, China). Cells were transfected with the miR-124a mimic or siRNAs using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. After 6 h, the cultures were replaced with fresh medium.

The pRL-TK Renilla luciferase reporter vector, pmirGLO luciferase reporter vector and Dual-luciferase reporter assay system were purchased from Promega (Madison, WI, USA). Reporter plasmids containing the 3′UTR of IQGAP1 (pmirGLO-IQGAP1) were co-transfected with the NC mimic or the miR-124a mimic into cells. pRL-TK Renilla luciferase reporter vector was used as an internal control. Luciferase activity was measured by the Dual-luciferase reporter assay system. Results were expressed as the firefly luciferase activity normalized to Renilla luciferase activity.

The MTT assay was used to assess cell proliferation. Cells were seeded in 96-well plates and allowed to grow for 24 h. After transfection, 50 μl MTT solution was added and incubated at 37°C for 4 h. After dissolving the formazine granulars with 150 μl DMSO, the optical density (OD) at 570 nm was measured using a microplate reader (Ascent 354; Thermo Labsystems, Waltham, MA, USA).

Transwell inserts (Corning) were coated with Matrigel (BD Biosciences, Franklin Lakes, NJ, USA) at 37°C for 30 min. The cells were suspended in serum-free medium at a final density of 5×10⁴ cells/ml and seeded to the upper chambers. Cell medium containing 10% FBS was added to the lower chambers. After incubation at 37°C for 12 h, non-invaded cells were removed by a cotton swab. The invaded cells were fixed in 95% ethanol for 20 min, followed by staining with hematoxylin for 10 min. The number of invaded cells was counted under an inverted microscope (Nikon).

The Student’s t-test was used to analyze differences between two groups. Data are expressed as the mean ± SD of at least three independent experiments. P<0.05 was defined as statistically significant.

The expression level of miR-124a in the clinical tissue specimens was determined by real-time PCR. The clinical glioma tissue samples were divided into two groups: low grade gliomas (grades I–II) and high grade gliomas (grades III–IV). As shown in Fig. 1A, the expression level of miR-124a in the glioma tissues was decreased when compared with the level in the normal tissues, and the expression level of miR-124a in the high grade gliomas was lower than that in the low grade gliomas.

We detected the expression level of miR-124a in a series of human glioma cell lines (U251, U343, U87, SF126, SF767). The results revealed that the expression level of miR-124a was lowest in the highly malignant glioma cell line U87 (Fig. 1B).

Expression of IQGAP1, β-catenin and phospho-β-catenin in the glioma tissue samples was determined by western blot analysis. The results revealed that the relative protein levels of IQGAP1 and β-catenin were increased in the glioma tissues when compared with the levels in the normal tissues. However, the relative protein level of phospho-β-catenin in the glioma tissues was lower than that in the normal tissues (Fig. 1C).

In addition, expression levels of IQGAP1, β-catenin and phospho-β-catenin were examined in a series of human glioma cell lines. As shown in Fig. 1D, U87 cells had high levels of IQGAP1 and β-catenin, but a low expression level of phospho-β-catenin. Based on the low expression level of miR-124a and high expression level of IQGAP1, we conducted miR-124a restoration and IQGAP1 knockdown on U87 cells for the following study in vitro.

To investigate the effects of miR-124a restoration on glioma cells, the miR-124a mimic was transfected into U87 cells. As shown in Fig. 2A, the expression level of miR-124a was higher in the miR-124a mimic group than that in the control group.

The proliferation rate of the U87 cells was determined using the MTT assay. As shown in Fig. 2B, the cells transfected with the miR-124a mimic proliferated at a significantly lower rate compared with the blank and NC mimic-transfected cells. In addition, the cell invasion assay revealed that the number of invaded cells was significantly decreased in the miR-124a mimic group compared with the number in the NC mimic group (Fig. 2C).

U87 cells were transfected with siRNA to knock down IQGAP1 expression. As shown in Fig. 3A and B, IQGAP1 expression was successfully reduced by siRNAs as determined using real-time PCR and western blot analyses.

Next, cells transfected with siRNA#1 were subjected to the MTT and cell invasion assays. As shown in Fig. 3C and D, U87 cells with reduced IQGAP1 showed decreased cell proliferation and invasion ability in comparison to the control cells.

In order to further validate the relationship between miR-124a and IQGAP1, we carried out the IQGAP1 3′UTR reporter assay in U87 cells. The results revealed that luciferase activity was significantly decreased in the U87 cells transfected with the miR-124a mimic and wild-type IQGAP1-3′UTR, but no reduction was observed in the mutant IQGAP1-3′UTR (Fig. 4). The results demonstrated that IQGAP1 is a direct target of miR-124a.

We tested whether IQGAP1 knockdown could affect the expression of β-catenin and cyclin D1. As shown in Fig. 5A, western blot analysis revealed that IQGAP1 knockdown resulted in decreased expression of β-catenin and cyclin D1.

In addition, immunofluorescence staining showed that in the IQGAP1 siRNA group, β-catenin was localized in the cytoplasm, and the staining of β-catenin was weaker in the IQGAP1 knockdown cells compared with the staining in the control cells (Fig. 5C).

We examined the effects of miR-124a restoration on the expression of IQGAP1, β-catenin and cyclin D1. Western blot analysis demonstrated that miR-124a restoration led to decreased expression of IQGAP1, β-catenin and cyclin D1 (Fig. 5B).

As shown in Fig. 5C, immunofluorescence staining of β-catenin revealed that compared with the miR-124a NC-transfected cells, β-catenin was localized in the cytoplasm and the staining of β-catenin was weaker in the miR-124a mimic-transfected cells.