PD-L1 expression in tumors has been hypothesized to be associated with response to PD-1/PD-L1 blockade. In a phase I trial, Topalian et al firstly demonstrated an association between PD-L1 expression in tumor cells and objective response to nivolumab in multiple cancer types (16). They determined surface PD-L1 expression of pretreated tumor samples via IHC, with a cut-off value of 5% defined to be PD-L1-positive, and found that 9 out of 25 PD-L1-positive patients had an objective response to nivolumab, while none of the 17 PD-L1-negative patients had an objective response. The KEYNOTE-024 study revealed superior progression-free survival (PFS) and overall survival (OS) in a pembrolizumab treatment group vs. a platinum-doublet chemotherapy group in patients with advanced NSCLC and PD-L1 expression in at least 50% of tumor cells (29). Thus far, several clinical trials have been performed to compare the treatment efficiency of anti-PD-1/PD-L1 antibodies between PD-L1-positive and -negative tumors (6–11,17,21,30–43), which are summarized in Table SI. Despite different pretreatments and cut-off points to define PD-L1 positivity, these studies have largely supported a role for PD-L1 expression, either on tumor cells or on tumor-infiltrating immune cells, as a predictive biomarker of response to PD-1/PD-L1 blockade in a variety of tumors. Notably, by analyzing multiple tumor types, Taube et al determined that membranous PD-L1 expression by tumor cells and infiltrating immune cells was most abundant in melanoma, NSCLC and RCC; tumors that exhibit objective response to anti-PD-1 immunotherapy (44).

In addition to PD-L1 expression on tumor cells or tumor-infiltrating immune cells, other forms of PD-L1 can also predict response to anti-PD-1/PD-L1 therapy. A recent study by Chen et al revealed the presence of PD-L1 on the surface of exosomes released by melanoma cells (45). They found that a fold change in circulating exosomal PD-L1 >2.43 at weeks 3–6 was associated with an improved objective response rate (ORR), PFS and OS to pembrolizumab. Another study of NSCLC suggested that the baseline plasma soluble PD-L1 concentration, determined using the enzyme-linked immunosorbent assay method, was significantly associated with clinical benefit in nivolumab therapy (46). However, lower response rate and shorter OS were detected in patients with NSCLC and high plasma-soluble PD-L1 levels.

In numerous tumors, PD-L1 expression can be induced either via oncogenic drivers and transcriptional factors, or via cytokines produced by tumor-infiltrating immune cells (47). Thus, PD-L1 acts as a constitutive and adaptive immune resistance against antitumor immune responses. The predictive value of PD-L1 expression can be explained by the fact that inhibiting the PD-1/PD-L1 axis with therapeutic antibodies allows the host to overcome immune resistance and thereby activate the antitumor immunity.

Although the results suggest PD-L1 expression as a predictive biomarker, several clinical trials have repeatedly demonstrated that there is a small but definite proportion of PD-L1-negative patients who can also derive clinical benefit from PD-1/PD-L1 blockade (6,9,20,21). As summarized in Table SI, ORR to PD-1/PD-L1 antibodies in PD-L1-negative groups was revealed to be 20–40% in melanomas, 10–20% in NSCLC, and 5–20% in urothelial carcinomas. Brahmer et al even observed similar ORRs and survival outcomes between patients with PD-L1-positive and -negative squamous-cell NSCLC treated with second-line nivolumab, collectively revealing that there should be predictive biomarkers other than PD-L1 expression that can also determine the efficacy of PD-1/PD-L1 inhibitors (9). PD-L1 testing alone is insufficient for the selection of patients for anti-PD-1/PD-L1 immunotherapy. On the other hand, several studies indicated that anti-PD-L1 is somewhat less effective than anti-PD-1 therapy, which may be associated with slightly lower toxicity in cancer treatment (16,48). This discrepancy is potentially due to the mode of action, targeting the ligand vs. the receptor, between anti-PD-1 and anti-PD-L1 antibodies. Consistently, our data also revealed that anti-PD-1 therapy, but not anti-PD-L1, was effective against FXR^highPD-L1^low mouse Lewis lung carcinoma (LLC) tumors. It speculated that the absence of targetable PD-L1 on tumor cells may be responsible for the ineffectiveness of anti-PD-L1 antibody (49). To date, no clinical trials have directly compared the treatment efficiency and toxicity between anti-PD-1 and anti-PD-L1 antibodies, particularly in PD-L1-low/negative patients.

Notably, the application of PD-L1 testing via IHC as a predictive biomarker is associated with several issues. Technically, different PD-L1 IHC antibodies with different analysis systems and different cut-off values for PD-L1 positivity were employed in early clinical trials (Table SI). The anti-PD-L1 28-2 clone and 22C3 clone were, thus far, the most prevalent antibodies for IHC. The common thresholds for PD-L1 positivity were 1, 5 and 10% in multiple cancer types (Table SI). Encouragingly, recent studies have compared three PD-L1 diagnostic assays (Dako 28-8, 22C3 and Ventana SP263), revealing preferable concordance rates at various cut-offs in resected NSCLC samples (50,51), and great efforts have been paid to develop a consensus for the use of PD-L1 IHC testing as a predictive biomarker for anti-PD-1/PD-L1 immunotherapy (52). Essentially, the expression of PD-L1 on tumor cells and infiltrating immune cells is dynamic. It has been discovered that PD-L1 expression levels can be influenced by various mechanisms, including the change in intracellular transcriptional factors or extracellular inflammatory cytokines, as well as antitumor therapies, such as radiation therapy, chemotherapy and targeted therapy (53–62). Recently, we detected an inverse correlation between FXR and PD-L1 expression in a cohort of NSCLC specimens (49). Our data demonstrated that FXR could directly bind to an FXR-responsive element in PD-L1 promoter and repress its transcription, suggesting FXR as a novel transcriptional factor for the regulation of PD-L1. In general, contemporaneous tumor samples, obtained at the beginning of immune checkpoint blockade (ICB), should represent the PD-L1 expression status better compared with archival tumor samples. Another ineluctable variable is the heterogeneity of PD-L1 expression, which exists both within the same tumor lesion, and between primary and metastatic lesions in the same patient. It has been reported that PD-L1 expression is widely heterogeneous within the tumor, which often accumulates at the tumor-immune interface (44). Takamori et al compared PD-L1 expression between lung metastases and corresponding primary tumors, including tumors in the rectum, colon, liver and bile duct. Although the proportion of PD-L1-positive tumor cells was not significantly different between lung metastases and primary tumors, PD-L1 expression on immune cells was significantly higher in lung metastases compared with the corresponding primary tumors (63). In this regard, tumor sampling aimed at a particular proportion of one tumor site may not accurately reflect on the PD-L1 status of that patient. Finally, factors enabling the prediction of response, such as tumor mutational burden (TMB) and the tumor microenvironment (TME), should be incorporated with PD-L1 IHC staining, in order to achieve a paradigm of precise anti-PD-1/PD-L1 immunotherapy.

Owing to advances in DNA sequencing techniques, a large amount of information on cancer genetics and genomics has been gained in the past few decades. There is increasing evidence that the TMB can predict response to ICIs, including anti-PD-1/PD-L1 drugs. The first indication was from the combined result, revealing that tumors with a high TMB (melanoma and NSCLC) often have a higher response rate to anti-PD-1 or anti-PD-L1 therapy across multiple tumor types (16,48,64–66). Moreover, within certain tumors, Rizvi et al revealed that patients with NSCLC and a high non-synonymous TMB exhibited durable clinical benefit to pembrolizumab, defined as a partial or stable response rate, for >6 months, compared with patients with less frequent non-synonymous mutations (67). Consistently, a pilot study of nivolumab in early-stage NSCLC revealed a significantly higher mean TMB in tumors with a major pathological response compared with tumors without a major pathological response (68). Recently, Wang et al reported that a higher blood TMB (bTMB), established by a 150-cancer gene panel of circulating tumor DNA, was significantly associated with superior PFS and ORR in patients with NSCLC treated with anti-PD-1 and anti-PD-L1 drugs (69), collectively suggesting the potential predictive value of TMB in antitumor PD-1/PD-L1 blockade.

Notably, recent evidence suggests that mismatch repair deficiency (MMRD) may be associated with an increased response to PD-1/PD-L1 blockade (70). Mismatch repair (MMR) is an intrinsic mechanism that can identify and correct errors in DNA replication and recombination, such as miss-incorporation deletions and base insertions (71). Mutations in MMR genes can produce log-fold increase of TMB, leading to the detectable DNA microsatellite instability (MSI) (23). It was estimated that tumors with a MMRD genotype possess 10 to 100-fold the mutational load of their MMR-proficient counterparts (23). Le et al have formally evaluated the predictive value of MMRD in patients with colorectal and non-colorectal cancer treated with pembrolizumab, and revealed that patients with MMRD colorectal cancer had a significantly improved ORR and PFS rate compared with those with MMR-proficient colorectal cancer (72). Moreover, it was demonstrated that the immune-associated ORR and PFS rate of pembrolizumab treatment were similar between MMRD colorectal cancers and MMRD non-colorectal cancers. Whole-exome sequencing revealed a mean of 1782 somatic mutations per tumor in MMRD tumors compared with 73 in MMR-proficient tumors; however, there were in total 41 cases in this phase II trial. Subsequently, the study was expanded to 86 patients of 12 tumor types with MMRD, which displayed an objective radiographic response rate of 53% and a complete response rate of 21% to anti-PD-1 therapy, although the median PFS and OS were not reached (73). Large clinical studies are required to verify the potential of MMRD or MSI in predicting the response to different PD-1/PD-L1 antibodies within different tumor types.

The association between TMB and response to anti-PD-1/PD-L1 drugs is considered to be primarily due to the generation of neoantigens, as a result of somatic mutations in tumor cells. The theory is that tumors with a high mutational load often possess more neoantigens, which can be recognized as non-self epitopes by the immune system, thereby enhancing T cell responses against tumors, as well as killing tumor cells when the PD-1/PD-L1 axis is blocked (74). It has been documented that the sensitivity to PD-1 blockade in advanced NSCLC and melanoma was increased in tumors enriched for clonal neoantigens (75). In parallel, active T cells against clonal neoantigens were detected in tumors with durable clinical benefits. Another study revealed a significant correlation between higher neoantigen burden and improved treatment efficacy in patients with NSCLC treated with pembrolizumab (67). In addition, the increased PD-L1 expression in the context of certain mutations is proposed as another determinant. A recent study demonstrated that the activating mutations in Janus kinase 3 (Jak3) promoted PD-L1 expression in lung cancer cells and the tumor immune microenvironment, thus contributing to the durable clinical benefit from anti-PD-L1 therapy (76). To date, a variety of oncogenic mutations, such as EGFR, PTEN and ALK, have been reported to be associated with PD-L1 upregulation in tumor cells, although further investigation is required to better define the role of PD-L1 in TMB-associated response to anti-PD-1/PD-L1 immunotherapy (77–79). In this respect, it is also reasonable to speculate that the somatic mutations in tumor cells would have a broad effect on the tumor immune microenvironment; specifically, those affecting other immune checkpoints, cytokines and chemokines that may also determine anti-PD-1/PD-L1 therapeutic response. Further studies are warranted for a comprehensive examination of host immune make-up in tumors with somatic mutations in comparison with others.

TMB is a promising predictive biomarker, albeit with its own limitations. Although a number of studies correlate TMB with tumor response to anti-PD-1/PD-L1 drugs, there has been thus far, seldom numerical cut-off value of TMB that was formally defined (16,39,48,66,68). Rizvi et al established a cut-off point of 178 non-synonymous mutations per sample to predict durable clinical benefit in a discovery cohort of NSCLC treated with pembrolizumab (67). In the validation set, this cut-off point yielded a clinical benefit rate of 75% in patients harboring at least 178 mutations, compared with 14% in patients with <178 mutations. Another clinical trial defined ≥10 mutations per megabase in DNA sequences of tumor cells as high TMB (34). Moreover, while somatic mutations in tumor cells may produce neoantigens that are prone to immune attack, not all neoantigens can elicit a T-cell response. A previous study has revealed that only 10% of non-synonymous point mutations of an MC38 mouse tumor model could generate peptides capable of binding to MHC-I with high affinity, and only a proportion of these peptides, rather than the overall peptide load, were necessary to elicit immunogenicity (80). Despite recent technological advances, for example, whole exome sequencing or computational algorithm, it is still challenging to identify meaningful neoantigens that are responsible for T-cell responses. In addition, intratumor mutation heterogeneity should be another obstacle for predicting response to PD-1/PD-L1 blockade. McGranahan et al detected considerable intratumor neoantigen heterogeneity within NSCLC tumors (75). It was demonstrated in the same study that decreased intratumor neoantigen heterogeneity was correlated with improved OS of patients with lung adenocarcinoma. Therefore, mutations that arise early and are shared by most cancer cells in an individual should elicit more potent antitumor T-cell responses compared with mutations that arise later or are limited to a fraction of cancer cells. Lastly, several studies revealed primary resistance to anti-PD-1/PD-L1 in tumors with specific mutations, for example LKB1 loss, which may be ascribed to the impaired antitumor immune responses (81,82).

The TME consists of non-malignant stromal cells, such as cancer-associated fibroblasts (CAFs), bone marrow-derived cells and tumor-infiltrating immune cells, extracellular matrix, and the blood and lymphatic vascular networks (83). The tumor-infiltrating immune cells include macrophages, dendritic cells, natural killer cells, B cells, effector T helper cells, regulatory T cells (Treg) and cytotoxic T cells. The stromal cells aforementioned can release growth factors, matrix-degrading enzymes, cytokines and chemokines, responsible for either antitumor immune response or immunosuppressive response (84,85). In addition, both activating markers, such as MHC-II and CD86, and exhausting markers, such as PD-1, CTLA-4, LAG-3 and TIM-3, can be expressed in tumor-infiltrating immune cells, which collectively form a complex host of factors to either combat or promote tumor progression (86). Previous studies have revealed that the response of tumor cells to anti-PD-1/PD-L1 therapy is determined not only by intrinsic properties of tumor cells but also by cellular and molecular components of TME (87,88).

Firstly, the presence of tumor-infiltrating T cells was demonstrated to be associated with clinical benefit from anti-PD-1/PD-L1 therapy. Tumeh et al analyzed tumor samples from 46 patients with metastatic melanoma treated with pembrolizumab (89). Higher numbers of CD8-, PD-1- and PD-L1-expressing T cells were found at the invasive tumor margin and inside tumors in responding patients compared with non-responders. Ultimately, it was validated that the presence of CD8⁺ T cells at the invasive tumor margin serves as a potential predictive biomarker to anti-PD-1 therapy in melanoma. Chen et al revealed a modest association between CD8⁺, CD3⁺ and CD45RO⁺ T cells in pretreated tumor samples and responsiveness to PD-1 blockade in patients with advanced melanoma (90). Notably, this association became more significant after anti-PD-1 therapy. Another study on melanoma found that an increasing proportion of PD-1^highCTLA-4^high subset within tumor-infiltrating CD8⁺ T cells strongly correlated with objective response to anti-PD-1 therapy (91). In contrast, PD-1⁺ tumor-infiltrating T cells were significantly decreased in brain metastatic lesions compared with primary lung cancer, which was associated with a lower likelihood of objective response to anti-PD-1 in brain metastases (92). Consistently, our study revealed that aside from the downregulated PD-L1 expression, enforced FXR expression generated an immunosuppressive microenvironment in mouse LLC tumors, characterized by the inactivated and exhausted CD8⁺ T cells (shown as decreased TNFα⁺CD8⁺ T cells, as well as increased LAG-3 expression on CD8⁺ T cells), which was correlated with significant tumor growth inhibition in FXR-overexpressed LLC tumors treated with anti-PD-1 antibody (49). These findings collectively suggested that pretreated tumor-infiltrating T cells, particularly for the exhausted CD8⁺ T cells, can act as a promising predictive biomarker for PD-1/PD-L1 blockade (Fig. 2). This phenomenon can be logically ascribed to the pre-existing T cell-mediated antitumor immunity, which is restrained by the PD-1/PD-L1-induced suppression, but can be reactivated via PD-1/PD-L1 blockade. However, it was also proposed that intratumor T cells expressing multiple exhaustion markers may be irresponsive to anti-PD-1/PD-L1 drugs (93). Kim et al reported that VEGF-A could induce transcription factor TOX expression in T cells to drive exhaustion-specific transcription program, accounting for the resistance to PD-1 blockade in microsatellite-stable colorectal cancer (94). Another study even defined a threshold for PD-1 downregulation on tumor-infiltrating CD8⁺ T cells, for which the release of adaptive immune resistance could be achieved via PD-1 blockade (95). It was revealed that the functionality of PD-1^high T cells in resistant tumors failed to be rescued by anti-PD-1 therapy. All these lend credence to the theory that the less exhausted tumor-infiltrating CD8⁺ T cells, rather than the over-exhausted populations, are probably the determinants of response to anti-PD-1/PD-L1 immunotherapy. A future area of research should be to evaluate the predictive value of exhausted CD8⁺ T cells, of various phenotypes within TME, in PD-1/PD-L1-based immunotherapy.

Secondly, the immunosuppressive cell populations in TME could restrain the response to PD-1/PD-L1 blockade (Fig. 2). It has been documented that tumor-infiltrating myeloid cells and Treg cells are partially responsible for the development of anti-PD-1 resistance in mouse colorectal and mammary cancer (95). Davis et al revealed that the functional inhibition of myeloid-derived suppressor cells (MDSCs) could enhance the objective response to anti-PD-L1 treatment in T cell-inflamed mouse tumor models of head and neck cancers (96). In a transgenic mouse model of neuroblastoma, Mao et al revealed that while checkpoint inhibitors were insufficient in controlling mouse neuroblastoma growth, combining suppressive myeloid cell inhibitor with anti-PD-1/PD-L1 antibodies resulted in superior tumor control (97). In this regard, the inhibition of immunosuppressive cell subsets within TME represents a potential predictive biomarker or rational approach to enhance antitumor PD-1/PD-L1 blockade.

Thirdly, the molecular profiles of TME pre- or post-anti- PD-1/PD-L1 immunotherapy represent alternative biomarkers of response (Fig. 2). Indoleamine 2,3-dioxygenase (IDO), a rate-limiting enzyme in the degradation of tryptophan via the kynurenine pathway, plays a critical role in suppressing T-cell immunity within tumors (98). A recent study revealed that MSI colorectal cancer overexpressing IDO were more responsive to anti-PD-1 treatment compared with microsatellite-stable cancer (99). The IFN-related genes are also relevant in patient selection for anti-PD-1/PD-L1 therapies. In a phase I/II study of durvalumab, an ORR of 33% was revealed in NSCLC patients positive for IFN-γ mRNA and 8% in those negative for IFN-γ mRNA (100). Consistently, in another study on atezolizumab, pretreatment melanoma samples from responding patients had increased expression of IFN-γ and IFN-related genes compared with non-responding samples (66). In contrast, Zaretsky et al revealed that defects in IFN receptor signaling pathways resulted in acquired resistance to anti-PD-1 therapy in melanoma (81). In addition, in a study characterizing the gene expression profiles of RCC treated with anti-PD-1 antibodies, immune genes such as BACH2, a regulator of CD4⁺ T cell differentiation, and CCL3 involved in leukocyte migration were revealed to be overexpressed in responding patients as compared with non-responders (101). Chen et al analyzed immune signatures in longitudinal tumor samples obtained at multiple time-points during anti-PD-1 therapies, and identified numerous genes to be differentially expressed between responders and non-responders (90). However, there is thus far no conclusive data on the predictive power of either IDO, or IFN-related genes, or other immune genes in TME for anti-PD-1/PD-L1 immunotherapy.

The close association between the aforementioned TME components and treatment efficacy in PD-1/PD-L1 blockade can be explained by the following theory. Immune recognition of tumors results in a host-immune response, which promotes tumor eradication through immune mechanisms, including antigen presentation, T cell priming and trafficking, cytokine production, cytotoxic activity and the expression of other immune genes. However, the antitumor Th1 and CD8⁺ T cell responses are negatively regulated by PD-1/PD-L1-mediated adaptive immune resistance (89). Other immunosuppressive constituents also contribute to the adaptive immune resistance. This is supported by the combined result, revealing that the upregulation of PD-L1 and IDO in response to IFN-γ promotes the development of tumor-infiltrating myeloid cells and Treg cells, thereby facilitating tumor immune evasion (102–104). Response to anti-PD-1/PD-L1 immunotherapy occurs primarily in cancer patients with a T cell-inflamed but adaptive immune-resistant TME (105). In addition, the potential interactions between TME components and PD-L1 expression have partially been disclosed before. PD-L1 expression in tumors represents a negative feedback to IFN-γ released by tumor-infiltrating immune cells, including macrophages, dendritic cells, natural killer cells, B cells and effector T helper cells (56,106). Other immune factors within TME, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-12, IL-4, IL-10 and transforming growth factor-β (TGF-β), have also been revealed to induce the expression of PD-L1 (57–59,107–109). In return, PD-L1 was documented to compromise the effector T-cell responses, promote the differentiation of induced Treg cells, as well as mediate the immunosuppressive activity of myeloid cells in tumors (89,102,103).

Traditionally, the immune profiles of tumors can be classified into three main phenotypes: The immune-inflamed phenotype, the immune-excluded phenotype and the immune-desert phenotype; based on whether tumors harbor an inflammatory TME or not (93). In this scenario, the immune-inflamed phenotype is postulated to correlate with a higher response rate to anti-PD-1/PD-L1 therapy. Recently, a new theory arose that according to both tumor-infiltrating lymphocytes (TILs) and PD-L1 expression in tumors, the TME can be stratified into four groups: TILs⁻PD-L1⁻, TILs⁻PD-L1⁺, TILs⁺PD-L1⁻ and TILs⁺PD-L1⁺ (88). While the TILs⁻PD-L1⁻ group may exhibit lack of response to anti-PD-1/PD-L1 antibodies, and the TILs⁺PD-L1⁻ group is irresponsive to anti-PD-L1, the TILs⁺PD-L1⁺ group would expect the best response to anti-PD-1/PD-L1 treatments. An alternative classification of the TME immune types provided by Kondou et al was determined by the expression level of the PD-L1 and CD8b genes (110). The aforementioned stratifications should enable the selection of optimal treatment strategies for patients with cancer. More recently, the effects of TME on PD-L1 expression have attracted much attention, particularly for those with a pre-existing T cell-inflamed phenotype. The adaptive CD8⁺ T cells, CAFs, M2-like macrophages, and corresponding cytokines were revealed to engage in the dynamic change of PD-L1 within local tumors (106,108,111). These findings support the predictive value of TME-derived cellular and molecular elements as a whole population, rather than in individuals, for anti-PD-1/PD-L1 immunotherapy. With the discovery of more determinants for ICB in the past decade, more attention is expected to be devoted to evaluating a spectrum of ‘immunome’ before anti-PD-1/PD-L1 therapies.

Immunogenic cell death (ICD) in tumors has been implicated in the response to PD-1/PD-L1 blockade. Recently, Zhao et al reported that irreversible electroporation could induce ICD in pancreatic ductal adenocarcinomas, which activated dendritic cells and alleviated stroma-induced immunosuppression (112). The combination of irreversible electroporation and anti-PD-1 resulted in a significantly longer median survival in mouse orthotopic pancreatic ductal adenocarcinoma models compared with mice administered either treatment alone. Another study revealed that the combination of cisplatin and high-dose crizotinib induced ICD in NSCLC cells, which synergized with anti-PD-1 to induce a superior cure rate and long-term survival in mouse orthotopic NSCLC models (113). It is considered that the dying tumor cells may function as tumor vaccines to stimulate antitumor immune responses during ICD.

The diverse T-cell repertoire, corresponding to different antigenic peptides bounding to class I or II MHC, is generated by random recombination of discrete TCR-αβ gene segments (114). Tumeh et al analyzed tumor samples from 46 patients with metastatic melanoma obtained before and during anti-PD-1 therapy (89). It was revealed that patients who met the criteria for radiographic response had more than 10 times as many TCR clones expansion after pembrolizumab treatment, suggesting that the TCR clonality may represent a promising on-treatment predictive biomarker for anti-PD-1 therapies. The predictive value of TCR clonality during anti-PD-1 can be interpreted as an enrichment of a diverse T-cell repertoire, which will eventually be tailored as antigenic peptides on MHC interaction with TCR, reflecting an activated immune response against tumor cells (115).

Recently, intensive studies have been conducted to dissect the impact of the gut microbiome on response to anti-PD-1/PD-L1 immunotherapy in human malignancies, including melanoma, hepatocellular carcinoma, gastric cancer and NSCLC (116–119). It has been acknowledged that patients responding to PD-1/PD-L1 blockade often harbor higher diversity of gut microbiome compared with non-responders (116,118,119). This effect can be partially attributed to the ability of the gut microbiome to activate the host innate immune responses (120). In addition, subsequent studies revealed a clearer association between an intact commensal bacterial community and robust antitumor T-cell responses (121,122). Other studies revealed that interventions to modulate gut bacterial profiles exhibited great promise for improving the therapeutic responses (121,123). Dong et al reported that diosgenin, a natural steroidal saponin, could modulate the composition of the intestinal microbiome in melanoma-bearing mice, thereby enhancing the growth-inhibitory effect of anti-PD-1 antibody (123). Another study revealed that oral administration of Bifidobacterium collaborated with anti-PD-L1 therapy to control tumor growth in mouse melanoma models (121). Although promising, the favorable bacterial species that improve antitumor PD-1/PD-L1 blockade remain to be determined.

The evaluation of peripheral blood could be another interesting approach. It has been reported by Weide et al that relative eosinophil count (≥1.5%), relative lymphocyte count (≥17.5%), LDH level (≤2.5-fold elevation), and the absence of metastases other than soft-tissue/lung were confirmed as independent baseline characteristics associated with favorable OS in patients with melanoma treated with pembrolizumab (124). Another study reported that low neutrophil to lymphocyte ratio and absolute neutrophil count during treatment was correlated with superior objective response and treatment duration in patients with advanced NSCLC cured by PD-1/PD-L1 inhibitors (125). Similarly, data from a study on patients with advanced/metastatic melanoma treated with nivolumab or pembrolizumab revealed that patients with an increased baseline LDH had a significantly shorter OS compared with those with normal LDH. Furthermore, patients with a relative increase of >10% from elevated baseline LDH during treatment had a significantly shorter OS compared with those with ≤10% change (126). Other parameters, such as peripheral Treg cells, antigen-specific CD8⁺ T cells and MDSCs, were also revealed to be associated with response to nivolumab in patients with melanoma (21,127). These factors may be associated with adaptive immune resistance, which can be overcome when the PD-1/PD-L1 axis is blocked, although the detailed mechanisms need to be elucidated in future. In clinical practice, these peripheral blood biomarkers have the advantage of being readily assessable, and are suitable for serial sampling during treatment.

Owing to advances in technology, medical imaging can not only assess macroscopic features, but also characterize the cellular and molecular properties of malignant lesions, which may serve as a novel approach to select patients for PD-1/PD-L1 blockade. It has recently been revealed that PD-L1 and PD-1 expression in NSCLC could be quantified non-invasively with PET-CT imaging using the radiotracers ¹⁸F-BMS-986192 and ⁸⁹Zr-nivolumab, respectively (128). Bensch et al conducted another first-in-human study characterizing the biodistribution of zirconium-89-labeled atezolizumab via PET within 22 patients across three tumor types (129). Notably, they found that zirconium-89-labeled atezolizumab tumor uptake was positively correlated with ORR, PFS and OS of enrolled patients treated with atezolizumab. TILs can also be traced by medical imaging. In a retrospective study by Sun et al a radiomic signature that included eight variables was established for assessing tumor-infiltrating CD8⁺ T cells in solid cancer types. Their data revealed that a high baseline radiomic score was associated with a higher proportion of patients who had an objective response at 3 or 6 months, as well as associated with improved OS either in univariate or in multivariate analysis (130). These imaging biomarkers were designed to detect and monitor antitumor immune activities within tumors, thereby predicting response to PD-1/PD-L1 blockade. Although just the beginning, image-driven biomarkers have the unique advantage of being non-invasive, which should make them promising candidates to aid future anti-PD-1/PD-L1 immunotherapy.