ETM Experimental and Therapeutic Medicine 1792-0981 1792-1015 D.A. Spandidos 10.3892/etm.2019.8199 ETM-0-0-8199 Articles Comparing the response of pulse oximetry and regional cerebral oxygen saturation to hypoxia in preschool children LuYi DiMeiqin LiChan ChenMengmeng YuanKaiming ShangguanWangning Department of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China Correspondence to: Dr Wangning Shangguan, Department of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, 109 West Xueyuan Road, Wenzhou, Zhejiang 325027, P.R. China, E-mail: sgwning@163.com 01 2020 14 11 2019 19 1 353 358 12042019 15102019 Copyright © 2019, Spandidos Publications 2019

Pulse oximetry and measurement of regional cerebral oxygen saturation (rcSO2) are used to monitor peripheral and cerebral oxygenation, respectively. However, the response of rcSO2 and pulse oxygen saturation (SpO2) to hypoxia in preschool children has not been previously assessed. A total of 36 preschool patients who had undergone a tonsillectomy [age, 4–6 years, American Society of Anesthesiologists grade I or II] were screened and prospectively enrolled in the present study. Hemodynamics, including rcSO2, SpO2, non-invasive blood pressure, heart rate, electrocardiogram and capnography, were continuously monitored throughout the study. Following pre-oxygenation, pressure-controlled ventilation with 100% oxygen was administered through a mask with a flow rate of 6 l/min, under total intravenous anesthesia, and the end-tidal carbon dioxide partial pressure was maintained between 30 and 40 mmHg. Tracheal intubation was then performed and ventilation was paused until SpO2 decreased to 90% or rcSO2 decreased by >10% of the baseline level. The duration from pausing of mechanical ventilation to the start of the rcSO2 decline was shorter than that of SpO2 (80.2±23.6 sec vs. 124.4±20.5 sec; P<0.001). Subsequent to the recovery of ventilation, the duration from the starting point to the increasing point of the baseline of rcSO2 was longer than that of SpO2 (84.8±24.3 sec vs. 15.2±6.8 sec; P<0.001). From the point where mechanical ventilation was paused to when rcSO2/SpO2 began to decrease, the rcSO2 and SpO2 values decreased and a significant correlation of them was observed (Pearson's correlation coefficient=0.317; P=0.027). From the time-point where mechanical ventilation was recovered to the time-point where rcSO2 or SpO2 began to increase, rcSO2 and SpO2 values decreased and a significant correlation of them was observed (Spearman's correlation coefficient=0.489; P=0.006). From the baseline to the minimum value, compared with the SpO2, the rcSO2 declined at a decreased rate (9.7±0.5% vs. 5.3±2.7%; P<0.001). The present clinical trial was registered at http://www.chictr.org.cn on 14th March 2016 (registration no. ChiCTR-OOC-16008095).

 hypoxia pulse oxygen saturation regional cerebral oxygen saturation children Introduction

Consecutive and non-invasive pulse oxygen saturation (SpO2) may be measured using pulse oximetry, which allows for rapid identification of hypoxic state. Therefore, this technique is a useful clinical alternative to intermittent arterial blood sampling (1). However, measurement of SpO2 has certain limitations and is difficult to use in the presence of nail polish, anaemia, light interference, skin pigmentation, venous pulsations and low perfusion, as they may cause measurement errors (2). In 1977, Jöbsis (3) introduced, for the first time, the monitoring of regional cerebral oxygen saturation (rcSO2) via near-infrared spectroscopy (NIRS). NIRS takes advantage of the tissue penetration abilities of light of the near-infrared spectrum. In contrast to SpO2, rcSO2 does not require plethysmography, and pulsatile flow measurement is also not required. NIRS assumes a relative and fixed amount of arterial vs. venous blood to determine the oxygen saturation. Therefore, rcSO2 does not provide an indicator of oxygen delivery and instead provides information regarding the balance between regional oxygen supply and demand (4). Recent studies have suggested that pediatric patients may benefit from rcSO2 monitoring during surgery (5–9). The use of rcSO2 is increasing, but the routine use of rcSO2 as a standard-of-care monitor is still not recommended at present.

Although it has been reported that rcSO2 provides an earlier alert during hypoxia compared with pulse oximetry (10), whether SpO2 and rcSO2 exhibit similar response curves during acute apnea has, to the best of our knowledge, not yet been reported in preschool children. The purpose of the present study was to determine whether a correlation is present between the changing tendency of SpO2 and rcSO2 in response to hypoxia in preschool patients. It was hypothesized that SpO2 may exhibit the same response to hypoxia as rcSO2.

 Materials and methods <sec> <title>Ethical approval and consent to participate

The present study was registered in the research registry (www.chictr.org.cn; registration no. ChiCTR-OOC-16008095; 14 March 2016). The protocol (no. 2016-08; 1 March 2016) was approved by the review board of the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University (Wenzhou, China). Written informed consent had been obtained by the parents or legally authorized guardians.

 Inclusion criteria

A total of 36 pediatric patients [age, 4–6 years; American Society of Anesthesiologists (ASA) grade I or II], scheduled for elective tonsillectomy between May and September 2016 at the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University were enrolled in the present clinical trial.

 Exclusion criteria

Patients were excluded if they exhibited the following: i) No cooperation; ii) body mass index of <13.5 kg/m2 or >31 kg/m2; iii) upper airway infection; iv) serious respiratory and/or cardiovascular disease, hepatic or renal insufficiency (the values of alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen, creatinine >1.5 times the upper limit of the normal level); v) asthma or airway hyperresponsiveness, neuromuscular diseases or cachexia; vi) airway abnormalities and a previous history of an abnormal response to anesthesia; vii) an acid-base imbalance or severe electrolyte disorder; viii) participation in another clinical study within 30 days.

 Experimental design

After arrival in the operating room, intravenous access was established into the peripheral vein in the forearm for induction of anaesthesia. Throughout the present study, all patients were continuously monitored, with their rcSO2 (SenSmart™; Nonin Medical, Inc.) being assessed using a cerebral oximetry probe (reading rcSO2 every 5 sec), which was placed on the middle of the forehead, and SpO2 being assessed using an oximetry probe (M1133A; Philips Medical Systems, Inc.), which was placed on the right index finger. Non-invasive systolic blood pressure (SBP), mean arterial pressure (MAP) and diastolic blood pressure (DBP) were measured every 1 min on a different limb to the SpO2 probe. Heart rate (HR), electrocardiogram and end-tidal carbon dioxide partial pressure (PETCO2) were also continuously monitored. Induction of anaesthesia was performed using propofol 2–3 mg/kg, fentanyl 2–3 µg/kg and cisatracurium 0.1–0.2 mg/kg. Anaesthesia was maintained with a continuous target-controlled infusion of propofol and remifentanil. Pressure-controlled ventilation of 100% oxygen through a mask, with a flow rate of 6 l/min, was administered, and PETCO2 was maintained between 30 and 35 mmHg. After a period of 6 min, mechanical ventilation was stopped and the tracheal tube was successfully introduced using a video laryngoscope. The tracheal tube was subsequently disconnected from the circuit and the proximal end was opened until the SpO2 decreased to 90% or until the rcSO2 decreased by >10% of the baseline level. The tracheal tube was then reconnected to the circuit and ventilation was recovered with a flow rate of 6 l/min of 100% oxygen.

The values of NIBP, HR, SpO2 and rcSO2 were recorded at the designated time-points: T0 indicates the time-point prior to application of oxygen prior to oxygenation; T1 indicates baseline, the time-point at which the mechanical ventilation was stopped; T2 indicates the time-point at which SpO2 began to drop from the baseline level; t2 indicates the time-point at which rcSO2 began to drop from the baseline level; T3 indicates the time-point of SpO2 decreasing to 90% or rcSO2 decreasing by >10% of the baseline level and mechanical ventilation being recovered; T4 indicates the time-point at which SpO2 began to rise from the minimum value following ventilation; t4 indicates the time-point at which rcSO2 began to rise from the minimum value following ventilation; T5 indicates the time-point at which SpO2 returned to the baseline level, t5 indicates the time-point at which rcSO2 returned to the baseline level. ST1-T4 indicates the value of SpO2 at T1 (baseline)-the value of SpO2 at T4 (the minimum value); RT1-t4 indicates the value of rcSO2 at the T1 time-point (baseline)-the value of rcSO2 at t4 (the minimum value; Fig. 1).

 Statistical analysis

All data were expressed as the mean ± standard deviation or as n (%), as appropriate. Statistical analysis was performed using SPSS 18.0 (SPSS Inc.). The calculation of the sample size, besides being based on the pilot study, mainly referred to that in previous studies (Koch et al (8), where the sample size was n=21, and the authors studied the perioperative use of cerebral and renal near-infrared spectroscopy in neonates; and Eichhorn et al (11), where the sample size was n=10, and a clinical trial was used to evaluate the use of near-infrared spectroscopy under apnea-dependent hypoxia in humans).

The normality of distribution of data was examined using the Shapiro-Wilk test. For the data that did not exhibit a normal distribution, a Wilcoxon signed-rank test and Spearman's rank correlation were used. Data exhibiting a normal distribution were analyzed using a repeated-measures one-way analysis of variance and Pearson's linear correlation. P<0.05 was considered to indicate statistical significance.

 Results <sec> <title>Patient characteristics

Among the 36 pediatric patients considered for the present study, 6 cases were excluded due to upper airway infection or body mass index >31 kg/m2, which may have added complexity to the procedure. Finally, a total of 30 patients, including 21 males and 9 females (age, 4.9±0.8 years; body weight, 21.8±5.5 kg) were enrolled in the present study.

 Vital signs at different time-points

Compared with the values at T0, the SBP, MAP and DBP were decreased at the time-points from T1 to T5/t5, and the HR was decreased at the T1 time-point (P<0.001). Compared with those at T1, the MAP and DBP were increased at the T2 time-point and the HR was increased from the T2/t2 to the T5/t5 time-point (P<0.001), as presented in Table I.

 Changes of rcSO<sub>2</sub> and SpO<sub>2</sub> over time

The values for rcSO2 and SpO2 are provided in Table II and the different time-intervals are stated in Table III. Compared with the SpO2, the rcSO2 exhibited an earlier decrease in response to hypoxia (t2-T1=80.2±23.6 sec vs. T2-T1=124.4±20.5 sec; P<0.001). However, the rcSO2 decreased slower than the SpO2 (T3-t2=104.8±27.3 sec vs. T3-T2=60.6±13.7 sec; P<0.001). Furthermore, the decrease of SpO2 to 90% of the baseline occurred earlier than that of rcSO2 decreasing by >10% of the baseline in all thirty cases. After the recovery of ventilation, rcSO2 was increased earlier than SpO2 (t4-T3=13.4±6.2 sec vs. T4-T3=18.9±6.5 sec; P<0.001) and the duration of t5-t4 was longer than that of T5-T4 (84.8±24.3 sec vs. 15.2±6.8 sec; P<0.001). In addition, the duration of t5-T3 was longer than that of T5-T3 (98.2±24.3 sec vs. 34.1±6.8 sec; P<0.001). From T2/t2 to T3, the rcSO2 and SpO2 values exhibited a decrease and a significant correlation of the two parameters was determined (Pearson's correlation coefficient=0.317; P=0.027). From T3 to T4/t4, the rcSO2 and SpO2 values decreased significantly and a significant correlation of the two parameters was obtained (Spearman's correlation coefficient=0.489; P=0.006), as shown in Figs. 2 and 3. Compared with ST1-T4, RT1-t4 was smaller (9.7±0.5 sec vs. 5.3±2.7%; P<0.001; Fig. 4).

 Discussion

The results of the present study demonstrated that rcSO2 and SpO2 exhibited similar dynamics in their changing curve patterns in response to acute apnea (no ventilation), although rcSO2 decreased earlier and declined slower than SpO2 during hypoxia. Furthermore, rcSO2 increased earlier and slower than SpO2 following the recovery of ventilation.

It has been previously suggested that apneic episodes in infants, which are known to cause an increase in vascular resistance and a reduction of cerebral blood volume, may be avoided with a threshold of SpO2 >85% for cerebral circulation (12). A study performed by Gupta et al (13) reported that by increasing the vascular resistance where the threshold of SpO2 was 90%, hypoxic load reduced the blood circulation of the middle cerebral artery in normal healthy adults. Therefore, in the present study, the threshold of SpO2 was set at 90%. It has been reported that a decline of >25% from the baseline level, or the value of rcSO2 of <40%, may influence neurologic dysfunction and exhibit adverse outcomes (14). A reduction to the value of 50% or less or a decrease of 15–20% from the baseline has been used as a critical threshold for interventions (15,16). Therefore, in the present study, a 10% reduction of rcSO2 from the baseline was used as a threshold to ensure patients' safety.

The present study demonstrated that after pausing mechanical ventilation (acute apnea), the rcSO2 decreased earlier and declined slower than SpO2. A previous study revealed that with SpO2 maintained in the normal range, a decrease of >20% may be observed in cerebral oxygen saturation (17). Another study indicated that SpO2 readings were 10–15 sec delayed compared with rcSO2 readings in neonates (9). Similar results were also reported by Tanidir et al (18). In the present study, the decrease of rcSO2 occurred ~40 sec earlier than that of SpO2. Tobias (10) suggested that these changes may be associated with different ‘blood beds’, which are evaluated using monitors. It has been demonstrated that SpO2 only captures arterial oxy-hemoglobin saturation and measures saturation in the arterial bed, but there is a correlation of rcSO2 values with mixed venous (70%) and arterial (30%) oxygen saturations (3). In contrast to SpO2, rcSO2 depends on venous blood. The partial pressure of oxygen would decrease at an approximately equal rate in venous and arterial ‘blood beds’ during apnea. However, due to the lower venous partial pressure of oxygen, it would reach the bend of the oxy-hemoglobin dissociation curve more rapidly. Therefore, a decrease in the rcSO2 would occur first. During hypoxia, the decline of rcSO2 reflects a concurrent decrease in arterial oxy-haemoglobin saturation and a rise in venous deoxy-hemoglobin saturation (10). In addition, Rasmussen et al (19) indicated that cerebral NIRS oximetry responded poorly to changes in tissue oxygenation during hypotension that was induced by decreased preloading. This may be due to the increase in the artery-to-vein ratio that occurs following the decrease in oxygen delivery, which is due to arterial vasodilation and possibly cerebral venous collapse. This may cause the arterial part of the NIRS signal to increase, leading to rcSO2 values decreasing more slowly. During the period of paused ventilation, the serum carbon dioxide increased and the blood vessels of the brain became dilated. Venous deoxy-hemoglobin saturation captured by rcSO2 may explain the early change in rcSO2. The effect of perfusion on rcSO2 levels has also been indicated by Schwaberger et al (20).

After restarting ventilation, rcSO2 was increased earlier than SpO2, but its increasing rate was slower, with rcSO2 and SpO2 exhibiting similar dynamic changing curve patterns. rcSO2 was increased with a mean delay of 13.4 sec, whereas the increase of SpO2 featured a significant delay of 18.9 sec. These results are similar to those of previous studies (9,11). It is well known that the brain responds to hypoxia through increasing cerebral blood flow. To maintain adequate oxygen supply in organs sensitive to hypoxia, including the brain, blood is being re-distributed (11,21,22). This may explain for the earlier increase of rcSO2 than that of SpO2 following the recovery of ventilation, as a result of the oxygenated blood preferentially being distributed to the brain. Delayed vasodilatation in the periphery, in comparison to the cerebral blood, may provide an additional explanation for the time difference observed between the increase of rcSO2 and that of SpO2 (11).

Of note, the present study has certain limitations. First, the sample size of the present study was relatively small. In addition, the experimental design was relatively simple and the further mechanism exploration was not included. In conclusion, during an episode of hypoxia, rcSO2 and SpO2 exhibited similar dynamics in their changing curve patterns, and rcSO2 was more sensitive compared with peripheral SpO2.

 Acknowledgements

The authors would like to thank Professor Daqing Ma, expert in Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London and Chelsea and Westminster Hospital (London, UK) for his critical comments provided throughout the preparation of the manuscript.

 Funding

No funding was received.

 Availability of data and materials

The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.

 Authors' contributions

WS and YL contributed to the design of the study and project administration. YL, CL and MD performed the experiments and analyzed the data. MC contributed to data analysis. KY performed the statistical analysis. YL and WS drafted, reviewed and edited the manuscript. All authors read and approved the final manuscript.

 Ethics approval and consent to participate

Ethical approval for this study was provided by the Ethical Committee of The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University (Wenzhou, China; no. 2016-08 dated 1 March 2016). Signed informed consent was obtained from the parents and/or guardians. Informed consent was provided by the parents or legally authorized guardians.

 Patient consent for publication

Not applicable.

 Competing interests

The authors have no competing interests to declare.

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Flow chart of the study. ASA, American Society of Anesthesiologists; NIBP, non-invasive blood pressure; HR, heart rate; SpO2, pulse oxygen saturation; rcSO2, regional cerebral oxygen saturation; PCV, pressure-controlled ventilation; T0, time-point prior to application of any oxygen for pre-oxygenation; T1, baseline, the time-point at which the mechanical ventilation was stopped; T2, the time-point at which SpO2 began to drop from baseline; t2, the time-point at which rcSO2 began to drop from baseline; T3, the time-point at which SpO2 decreased to 90% or rcSO2 decreased to by >10% of the baseline and mechanical ventilation was recovered; T4, the time-point at which SpO2 began to rise from the minimum value following ventilation; t4, the time-point at which rcSO2 began to rise from the minimum value following ventilation; T5, the time-point at which SpO2 returned to the baseline level, t5, the time-point at which rcSO2 returned to the baseline level.

Pearson's correlation scatter plot of T3- T2/t2. From T2/t2 to T3, the rcSO2 and SpO2 values exhibited a significant correlation (Pearson's correlation coefficient=0.317; P=0.027). T2, the time-point at which SpO2 began to drop from the baseline level; t2, the time-point at which rcSO2 began to drop from the baseline level; T3, the time-point of SpO2 decreasing to 90% or rcSO2 decreasing by >10% of the baseline level and mechanical ventilation being recovered.

Spearman's rank correlation scatter plot of T4/t4-T3. From T3 to T4/t4, the rcSO2 and SpO2 values exhibited a significant correlation (Spearman's correlation coefficient=0.489; P=0.006). T3, the time-point at which SpO2 decreased to 90% or rcSO2 decreased by >10% of the baseline and mechanical ventilation was recovered; T4, the time-point at which SpO2 began to rise from the minimum value following the ventilation; t4, the time-point at which rcSO2 began to rise from the minimum value following the ventilation.

Dynamic changes of SpO2 and rcSO2 during hypoxia. *P<0.001 vs. T1/T2, #P<0.001 vs. T2/T3, $P<0.001 vs. T3/T4, &P<0.001 vs. T4/T5, θP<0.001 vs. T3/T5. SpO2, pulse oxygen saturation; rcSO2, regional cerebral oxygen saturation T0, time-point prior to application of any oxygen for pre-oxygenation; T1, baseline, the time-point at which mechanical ventilation was stopped; T2, the time-point at which SpO2 began to drop from baseline; t2, the time-point at which rcSO2 began to drop from baseline; T3, the time-point at which SpO2 decreased to 90% or rcSO2 decreased by >10% of the baseline and mechanical ventilation was recovered; T4, the time-point at which SpO2 began to rise from the minimum value following ventilation; t4, the time-point at which rcSO2 began to rise from the minimum value following ventilation; T5, the time-point at which SpO2 returned to the baseline level, t5, the time-point at which rcSO2 returned to the baseline level.

Dynamic changes of SBP, MBP, DBP and HR at different time-points.

Parameter T0 T1 T2/t2 T3 T4/t4 T5/t5
SBP (mmHg) 112±12 94±11a 98±13a/97±12a 97±13a 97±12a/97±13a 98±13a/98±12a
MAP (mmHg) 80±9 62±8a 68±11ab/66±9ab 65±9a 65±9a/65±9a 66±10a/64±11a
DBP (mmHg) 64±11 46±9a 53±11ab/51±9ab 49±9a 49±9a/49±9a 50±10a/47±12a
HR (bpm) 96±16 83±13a 93±12b/91±10b 92±16b 92±16b/92±16b 91±16b/98±15b

All values are expressed as the mean±standard deviation (n=30). Compared with T0

P<0.001; compared with T1

P<0.001. SBP, systolic blood pressure; MAP, mean arterial pressure; DBP, diastolic blood pressure; HR, heart rate. T0, time-point prior to application of any oxygen for pre-oxygenation; T1, baseline, the time-point at which mechanical ventilation was stopped; T2, the time-point at which SpO2 began to drop from baseline; t2, the time-point at which rcSO2 began to drop from baseline; T3, the time-point at which SpO2 decreased to 90% or rcSO2 decreased by >10% of the baseline and mechanical ventilation was recovered; T4, the time-point at which SpO2 began to rise from the minimum value following ventilation; t4, the time-point at which rcSO2 began to rise from the minimum value following ventilation; T5, the time-point at which SpO2 returned to the baseline level, t5, the time-point at which rcSO2 returned to the baseline level.

Dynamic changes of SpO2 and rcSO2 at different time-points (n=30).

Item T0 T1 T2/t2 T3 T4/t4 T5/t5
SpO2 (%) 99.7±0.5 99.7±0.5 99.7±0.5 90±0.0 84.7±3.2 99.7±0.5
rcSO2 (%) 81.4±3.9 87.0±3.6 87.0±3.6 81.8±4.5 80.4±4.0 81.4±3.9

All values are expressed as the mean±standard deviation (n=30). Compared with ST1-T4, RT1-t4 was smaller (9.7±0.5% vs. 5.3±2.7%, P<0.001). SpO2, pulse oxygen saturation; rcSO2, regional cerebral oxygen saturation; ST1-T4, the value of SpO2 at T1 (baseline)-the value of SpO2 at T4 (the minimum value); RT1-t4, the value of rcSO2 at the T1 time-point (baseline)-the value of rcSO2 at t4 (the minimum value). T0, time-point prior to application of any oxygen for pre-oxygenation; T1, baseline, the time-point at which mechanical ventilation was stopped; T2, the time-point at which SpO2 began to drop from baseline; t2, the time-point at which rcSO2 began to drop from baseline; T3, the time-point at which SpO2 decreased to 90% or rcSO2 decreased by >10% of the baseline and mechanical ventilation was recovered; T4, the time-point at which SpO2 began to rise from the minimum value following ventilation; t4, the time-point at which rcSO2 began to rise from the minimum value following ventilation; T5, the time-point at which SpO2 returned to the baseline level, t5, the time-point at which rcSO2 returned to the baseline level.

Comparison of the time difference between SpO2 and rcSO2 during the response to hypoxia (sec).

Duration SpO2 rcSO2 P-value
T2/t2-T1 124.4±20.5 80.2±23.6 <0.001
T3-T2/t2 60.6±13.7 104.8±27.3 <0.001
T4/t4-T3 18.9±6.5 13.4±6.2 <0.001
T5/t5-T4/t4 15.2±6.8 84.8±24.3 <0.001
T5/t5-T3 34.1±6.8 98.2±24.3 <0.001

All values are expressed as the mean±standard deviation (n=30). SpO2, pulse oxygen saturation; rcSO2, regional cerebral oxygen saturation; T1, baseline, the time-point at which mechanical ventilation was stopped; T2, the time-point at which SpO2 began to drop from baseline; t2, the time-point at which rcSO2 began to drop from baseline; T3, the time-point at which SpO2 decreased to 90% or rcSO2 decreased by >10% of the baseline and mechanical ventilation was recovered; T4, the time-point at which SpO2 began to rise from the minimum value following ventilation; t4, the time-point at which rcSO2 began to rise from the minimum value following ventilation; T5, the time-point at which SpO2 returned to the baseline level, t5, the time-point at which rcSO2 returned to the baseline level.