Introduction
Acute pancreatitis (AP) is an inflammatory disorder that has been found to be associated with systemic inflammatory response syndrome and multiple organ dysfunction syndrome (1,2). In individuals with AP, apoptotic and necrotic cell death are two major pathways contributing to the severity and mortality of AP (3-5). Apoptotic cell death is considered to protect individuals with pancreatitis from a mild inflammatory response, while necrosis leads to systemic damage in the pathology of severe AP, due to the activation of digestive enzymes and other inflammatory mediators (4-7). Hence, approaches targeting necrosis may have great therapeutic potential for the treatment of severe AP.
Ferroptosis is an iron-catalyzed form of necrosis that is induced by two classes of small-molecule substances, including class 1 (system Xc− inhibitors) and class 2 ferroptosis inducers [glutathione peroxidase 4 (GPX4) inhibitors] (8,9). System Xc− consists of disulfide-linked heterodimers between cystine/glutamate transporter (xCT) encoded by the solute carrier family 7 member 11 (SLC7A11) gene, and the 4F2 cell-surface antigen heavy chain which is encoded by the solute carrier family 3 member 2 gene (SLC3A2), which are responsible for the import of cystine, a major component required for glutathione (GSH) synthesis (9). The inhibition of cystine import has been reported to lead to the depletion of intracellular GSH levels, which in turn inactivate GPX4 and increase lipid reactive oxygen species (ROS) production (9). Similarly, the knockdown of GPX4 has been also reported to promote rapid lipid ROS accumulation; however, these effects may be inhibited by lipophilic radical traps and iron chelators (10,11). Previous studies have indicated crucial roles for ferroptosis in various diseases, including cancer and renal failure (10,11). However, there are only a limited number of studies available to date on whether ferroptosis plays a key role in AP-related cell death (12,13). For instance, Ma et al (12) demonstrated that 24 h after AP, ferroptosis-related protein levels were markedly elevated. Ferroptosis has also been shown to participate in pancreatic dysfunction triggered by arsenic (13).
Ginsenosides are crucial active pharmaceutical components, isolated from the traditional Chinese medicine ginseng (14,15). The anti-diabetic effects of ginseng have been reported, as it has been reported to induce insulin secretion, stimulate glucose uptake, suppress the intestinal absorption of glucose, and decrease glycogenolysis (16,17). Ginsenoside Rg3 is an important active component of Panax ginseng, which has been documented to exert potent protective effects on hyperglycemia, obesity and diabetes by protecting against pancreatic β-cell death (18,19). However, to the best of our knowledge, not study to date has investigated whether ginsenoside Rg3 protects against acute AP.
In the present study, the effects of Rg3 on severe AP were first investigated in vivo, and an in vitro AP cell model was then established to evaluate whether Rg3 protects the cells against ferroptosis-related cell death in vitro.
Materials and methods
Cells and cell culture
Rat pancreatic acinar AR42J cells were purchased from the American Type Culture Collection (ATCC). The cells were cultured in F12K medium (HyClone; GE Healthcare Life Sciences) supplemented with 20% FBS (HyClone; GE Healthcare Life Sciences), 100 U/ml penicillin (HyClone; GE Healthcare Life Sciences) and 100 mg/ml streptomycin (HyClone; GE Healthcare Life Sciences) in a humidified atmosphere containing 5% CO2 at 37°C. The AR42J cells were pre-incubated with or without Rg3 (cat. no. HY-N0603; MedChemExpress) at 37°C for 1 h and then treated with cerulein (Cn; 10−8 M; cat. no. HY-A0190; MedChemExpress) for a further 24 h to examine the protective effects of ginsenoside Rg3 on AP.
Animals
Male C57BL/6 mice (8 weeks old; SPF; weighing 24-26 g, n=16 in total) were purchased from SPF (Beijing) Biotechnology Co., Ltd. All mice were housed in an environmentally controlled room at a temperature ranging from 20 to 24°C on a 12 h light/dark cycle and used in the experiments following an overnight fast with water, available ad libitum. All procedures followed the Principles of Laboratory Animal Care (NIH publication number 85Y23, revised in 1996), and the experimental protocol was approved by the Animal Care Committee, Nanjing Medical University (NMU-2021JK-085).
In the present study, Cn was used to establish an in vitro model of AP according to previously published study protocols (20-22). All mice in each group fasted for 12 h prior to the experiment, with free access to water. Mice were administered eight intraperitoneal Cn injections (50 µg/kg) at hourly intervals to establish a model of AP (23), and saline-treated animals served as the controls. Based on a preliminary analysis, it was found that 20 and 40 mg/kg Rg3 obviously improved cell death in mice with AP induced by Cn (data not shown). The mice were randomly divided into four groups (the control group included 5 mice, the AP group included 5 mice, and the 20 and 40 mg/kg Rg3 treatment groups each included 3 mice; n=16 mice in total) as follows: The control, AP, AP + low-dose Rg3 (L-Rg3; 20 mg/kg) and AP + high-dose Rg3 (H-Rg3; 40 mg/kg). Rg3 (20 or 40 mg/kg) was intragastrically administered to the mice in the AP groups after the Cn injection daily for 2 weeks, whereas the mice in the other groups were administered normal saline (10 ml/kg) for 2 weeks. Animal health and behavior were monitored daily. No animal death occurred during the experiment. For anesthesia, all mice were anesthetized by an intraperitoneal injection of pentobarbital sodium (50 mg/kg). Subsequently, blood samples were collected through cardiac puncture and the mice were euthanized by exsanguination. Death was confirmed by determining the lack of heartbeat, pupillary response to light and respiration. The pancreas was then immediately dissected. A portion of the pancreas was fixed with 4% paraformaldehyde (Beijing Solarbio Science & Technology Co., Ltd.) in PBS (Beijing Solarbio Science & Technology Co., Ltd.) for 12 h for histological analysis. The remaining pancreatic tissue was stored at -80°C, until further investigation.
Pancreatic mass measurement
The wet mass of the pancreas was determined using an electronic balance (one ten-thousandth) (Secura, Sartorius Co. https://www.sartorius.com.cn/). The pancreas was then dried in a 60°C oven (Oven-91, LabCompanion, Jeio Tech Co., Ltd.) for 24 h, and the dry mass was measured using Electronic balance (one ten-thousandth) (Secura, Sartorius Co. https://www.sartorius.com.cn/). The pancreas moisture content was determined as follows: Water content of pancreas=(wet mass-dry mass)/wet mass ×100%.
Serum amylase assay
Blood was centrifuged at 4°C for 15 min at 3,000 × g, and serum amylase levels were determined using an Amylase Activity Assay kit (cat. no. MAK009; MilliporeSigma), according to the manufacturer's instructions.
Dichlorofluorescein diacetate (DCFH-DA) staining
Briefly, the AR42J cells (106 cells/well in a 6-well plate) were pre-incubated with or without Rg3, for 1 h, at 37°C and then treated with Cn (10−8 M; cat. no. HY-A0190; MedChemExpress) for 24 h at 37°C. The cells were stained with 5 µM DCFH-DA (cat. no. HY-D0940; MedChemExpress) in PBS in the dark for 30 min at 37°C and then observed under a fluorescence microscope (magnification ×20; IXplore; Olympus Corporation).
Annexin V/7-AAD assay
The AR42J cells (106 cells/well in a 6-well plate (Corning, Inc.) were pre-incubated with or without Rg3 for 1 h at 37°C and then treated with Cn (10−8 M, cat. no. HY-A0190, MedChemExpress) for 24 h. Cell death was quantified using an Annexin V-PE/7-AAD apoptosis kit (cat. no. AP104-30; Multi Sciences (LIANKE) Biotech, Co., Ltd.). Cells were washed with ice-cold PBS three times and resuspended in 500 µl Apoptosis Positive Control Solution [cat. no. AP104-30; Multi Sciences (LIANKE) Biotech, Co., Ltd.]. Following three washes with PBS, 1X binding buffer [cat. no. AP104-30; Multi Sciences (LIANKE) Biotech, Co., Ltd.] was added. Subsequently, the cells were stained with 5 µl Annexin V-PE [cat. no. AP104-30; Multi Sciences (LIANKE) Biotech, Co., Ltd.] and 10 µl of 7-AAD [cat. no. AP104-30; Multi Sciences (LIANKE) Biotech, Co., Ltd.], in the dark, for 5 min, at room temperature. The cells were then analyzed using a BD FACSCalibur flow cytometer (BD Biosciences), and data were analyzed using ModFit software version 4.1 (Verity Software House, Inc.). According to the instructions of the manufacturer [Annexin V-PE/7-AAD apoptosis kit; cat. no. AP104-30; Multi Sciences (LIANKE) Biotech, Co., Ltd.], quadrant (Q)3 represents early apoptotic cells, and Q2 represents late apoptotic and necrotic cells.
Cell Counting Kit-8 (CCK-8) assay
Briefly, the AR42J cells (3×103 cells/well, seeded in a 96-well plate) were pre-incubated with 10, 20, 40 or 80 µM Rg3 at 37°C for 1 h and then exposed to Cn (10−8 M, cat. no. HY-A0190, MedChemExpress) for 24 h. Subsequently, cell viability was determined using a CCK-8 (cat. no. HY-K0301, MedChemExpress), incubating the cells with 10 µl CCK-8 solution at 37°C for 4 h. Cell viability was then determined by measuring the optical density (OD) at 450 nm using a microplate reader (Thermo Fisher Multiskan; Thermo Fisher Scientific, Inc.).
Additionally, to further explore whether Rg3 alleviates AP in Cn-related cell death via ferroptosis, the AR42J cells we pre-incubated with various inhibitors, including a pan-caspase/apoptosis inhibitor (Z-VAD-FMK, 20 µM, MedChemExpress), a ferroptosis inhibitor [ferrostatin-1 (Fer-1), 1 µM, MedChemExpress], a necrosis inhibitor [necrostatin-1 (Nec-1), 20 µM, MedChemExpress] and an autophagy inhibitor [3-methyladenine (3-MA), 20 µM, MedChemExpress], for 2 h at 37°C. Subsequently, the cells were further treated in the presence of 20 µM Rg3 for a further 24 h. Cell viability was determined as described as above.
ELISA
The blood samples were centrifuged at 3,000 × g for 15 min and serum was collected to determine the levels of high sensitivity C-reactive protein (hs-CRP) using the hs-CRP ELISA kit (BKE8773, Shanhai boke Biotechnology Co. Ltd., Shanghai, China, https://b2b.baidu.com/shop?name=%E4%B8%8A%E6%B5%B7%E5%B8%9B%E7%A7%91%E7%94%9F%E7%89%A9%E6%8A%80%E6%9C%AF%E6%9C%89%E9%99%90%E5%85%AC%E5%8F%B8&xzhid=31870748&tpath=index&from=ent_card&prod_type=91) according to the provided instructions.
Quantification of malondialdehyde (MDA), ROS, GSH and ferrous ion (Fe<sup>2+</sup>) levels
The MDA, ROS, GSH and Fe2+ contents were determined using a Lipid Peroxidation MDA assay kit (cat. no. S0131S, Beyotime Institute of Biotechnology), ROS detection kit (cat. no. ML-Elisa-0255; R&D Systems, Inc.), GSH detection kit (cat. no. BC1175; Beijing Solarbio Science & Technology Co., Ltd.) and Iron assay kit (cat. no. MAK025; MilliporeSigma) according to the provided instructions.
Reverse transcription-quantitative PCR (RT-qPCR)
Total RNA was isolated from pancreatic tissues using RNAVzol (Vigorous Biotechnology Beijing Co., Ltd.) according to the manufacturer's protocol. The concentration and purity of the RNA samples were determined by measuring the OD at 260 and 280 nm using a microplate reader (Multiskan Spectrum, Thermo Fisher Scientific, Inc.). RT-qPCR was performed using the Takara PrimeScript™ One Step RT-PCR kit version 2.0 (cat. no. RR055A; Takara Bio, Inc.) according to the manufacturer's instructions. The following PCR mix was used: 20 µl RNase-free ddH2O, 25 µl 2X 1 step buffer, 2 µl PrimeScript™ 1 step enzyme mix, 1 µl upstream primer, 1 µl downstream primer and 1 µl RNA templates. All the reagents were included in the Takara PrimeScript™ One Step RT-PCR kit version 2.0 (cat. no. RR055A; Takara Bio, Inc.). Additionally, the following thermocycling conditions were applied: 50°C for 30 min; 94°C for 2 min; 30 cycles of 94°C for 30 sec, 55°C for 30 sec, and 72°C for 1 min; followed by 72°C for 10 min. GAPDH was used as an internal control. Relative mRNA expression was normalized to GAPDH using the 2−∆∆Cq method (24). The primers used in the present study are listed in Table I.
Western blot analysis
Protein was isolated from pancreatic tissues using a total protein extraction kit (Beijing Solarbio Science & Technology Co., Ltd.). A BCA protein assay kit (Pierce; Thermo Fisher Scientific, Inc.) was used for protein quantification. Subsequently, the protein samples (30 µg/lane) were loaded onto 12% SDS-PAGE gels, separated electrophoretically, and then transferred onto polyvinylidene difluoride (PVDF) membranes (Pierce; Thermo Fisher Scientific, Inc.). Subsequently, the proteins were blocked with 8% skim milk (Pierce; Thermo Fisher Scientific, Inc.) in 0.1% Tris-buffered saline (Beijing Solarbio Science & Technology Co., Ltd.) containing Tween-20 (TBST, Beijing Solarbio Science & Technology Co., Ltd.) for 2 h at room temperature. Following three washes with TBST (5 min/wash), the membranes were incubated with primary antibodies against nuclear factor erythroid 2-related factor 2 (NRF2; 1:1,000, cat. no. 20733, Cell Signaling Technology, Inc.), heme oxygenase 1 (HO-1; 1:1,000, cat. no. 43966, Cell Signaling Technology, Inc.), xCT (1:1,000; cat. no. ab175186; Abcam), GPX4 (1:1,000; cat. no. ab125066; Abcam), prostaglandin-endoperoxide synthase 2 (Ptgs2; 1:1,000, cat. no. 12282, Cell Signaling Technology, Inc.) and GAPDH (1:3,000, cat. no. 5174, Cell Signaling Technology, Inc.) overnight at 4°C. Subsequently, the membranes were incubated with an HRP-conjugated anti-rabbit IgG secondary antibody (1:5,000; cat. no. ZB-2301; OriGene Technologies, Inc.) at room temperature for 1 h. Immobilon Western Chemilum Hrp Substrate (WBKLS0500, MilliporeSigma) were used to visualize the antibody-antigen interactions. ImageJ 1.43b software (National Institutes of Health) was also applied for densitometric analysis.
TUNEL (TdT-mediated dUTP nick end labeling) staining
Pancreatic tissues were fixed in 4% phosphate-buffered neutral formalin (Beijing Solarbio Science & Technology Co., Ltd.) at room temperature for 20 min, embedded in paraffin and cut into 5-µm-thick sections, followed by deparaffinization, descending alcohol series of rehydration at room temperature. Sections were subsequently incubated with 0.3% hydrogen peroxide/phosphate-buffered saline for 30 min. Cell death was determined using a TUNEL Apoptosis Assay kit (Beijing Solarbio Science & Technology Co., Ltd.) according to the relevant instructions. Stained cells were counted in five random fields using light microscope (magnification, ×40; Olympus CK40; Olympus Corporation).
Statistical analysis
Statistical analyses of all quantitative data were performed with SPSS version 13.0 (SPSS, Inc.). Statistical analyses were performed using an unpaired Student's t-test for comparisons between two groups, and one-way analysis of variance followed by Tukey's post hoc test for comparisons of more than two groups. P<0.05 was considered to indicate a statistically significant difference.
Results
Rg3 reverses the Cn-induced death of rat pancreatic acinar AR42J cells
The results of CCK-8 assay revealed that Cn significantly decreased rat pancreatic acinar AR42J cell viability; however, pre-incubation with Rg3 markedly reversed these effects in a concentration-dependent manner (Fig. 1A). The AR42J cells were further treated with 20 and 40 µM Rg3. The results of CCK-8 assay also indicated that treatment with 40 µM Rg3 decreased the survival rate of the AR42J cells by ~25%; however, treatment with 20 µM Rg3 did not inhibit the survival rate of the AR42J cells (Fig. 1B). Hence, as 40 µM Rg3 may exert cytotoxic effects on AR42J cells and 20 µM Rg3 was thus used in the subsequent assays.
To further explore whether Rg3 alleviates AP in Cn-related cell death via ferroptosis, the AR42J cells we pre-incubated various inhibitors, including an apoptosis inhibitor (Z-VAD-FMK), a ferroptosis inhibitor [ferrostatin-1 (Fer-1)], a necrosis inhibitor [necrostatin-1 (Nec-1)], an autophagy inhibitor [3-methyladenine (3-MA)], as well as Rg3. As depicted in Fig. 1C, treatment with Cn decreased the cell survival rate to ~41%. In comparison with the cells treated with Cn only, pre-incubation with Z-VAD-FMK elevated the cell survival rate up to ~61%, Fer-1 increased the rate to ~96% and Rg3 increase the rate to ~88% (Fig. 1D). As demonstrated in Fig. 1D, the Q2 quadrant percentages of the control, Cn and Cn + Rg3 groups were 4.61, 26.6 and 13.6%, respectively. By contrast, the Q3 quadrant percentages of the control, Cn and Cn + Rg3 groups were 6.46, 3.97 and 7.66%, respectively. Hence, apoptosis was not the major form of Cn-induced cell death. These observations indicated that Rg3 contributed to the attenuation of AP in Cn-related cell death, mainly via ferroptosis.
Rg3 abolishes ferroptosis induced by Cn in rat pancreatic acinar AR42J cells
DCFH-DA staining revealed that compared with the control group, Cn increased ROS production in rat pancreatic acinar AR42J cells; however, pre-incubation with Rg3 decreased ROS production (Fig. 2A). Moreover, compared with the control, the intracellular MDA, ROS and Fe2+ contents were significantly increased in the AR42J cells exposed to Cn. However, the GSH levels were significantly reduced following exposure to Cn. By contrast, pre-incubation with Rg3 significantly reduced the MDA, ROS and Fe2+ levels, while increasing the GSH content in AR42J cells (MDA: 1±0.11 vs. 2.56±0.28 vs. 1.34±0.14; ROS: 1±0.09 vs. 1.67±0.21 vs. 1.18±0.11; Fe2+: 1±0.095 vs. 1.89±0.16 vs. 1.23±0.13; GSH: 1±0.087 vs. 0.63±0.15 vs. 0.87±0.11; for control vs. Cn vs. Cn + Rg3 group, respectively) (Fig. 2B-E). The mRNA expression levels of Ptgs2, an important ferroptosis marker, were also quantified. The Ptgs2 mRNA expression levels were significantly increased in the AR42J cells exposed to Cn; however, pre-incubation with Rg3 reduced the Ptgs2 mRNA expression levels (Fig. 2F). Furthermore, the GPX4 and xCT expression levels were significantly decreased following exposure to Cn. Pre-incubation with Rg3 increased GPX4 and xCT mRNA and protein expression in AR42J cells (Fig. 2F and G). Based on these data, pre-incubation with Rg3 reversed Cn-induced ferroptosis in AR42J cells.
Rg3 reduces cell death in mice with AP
As demonstrated in Fig. 3A and B, in comparison with the control, the weight of the pancreas was significantly increased in the mice with Cn-induced AP. However, treatment with high and low doses of Rg3 reduced the pancreatic weight in AP model mice (3.24±0.44 vs. 6.08±0.73 g vs. 4.44±0.49 g vs. 3.04±0.36 g for the control, AP, AP + L-Rg3 and AP + H-Rg3, respectively). Additionally, the serum amylase levels were significantly increased in AP model mice as compared with the control. Treatment with Rg3 decreased the serum amylase contents in mice with AP (1,861.35±303.36 vs. 11,042.32±528.03 vs. 5,302.62±425.7 vs. 2,808.25±625.1 U/l for the control, AP, AP + L-Rg3 and AP + H-Rg3, respectively) (Fig. 3C). The pancreatic moisture content was significantly increased in the AP group in comparison with the control group, whereas treatment with low and high doses of Rg3 significantly reduced the pancreatic moisture content in AP model mice (71.24±2.34 vs. 81.01±2.51 vs. 76.19±2.1 vs. 73.23±4.25% for the control, AP, AP + L-Rg3 and AP + H-Rg3, respectively) (Fig. 3D). TUNEL staining revealed an evident increase in cell death in the AP group; however, the high and low doses of Rg3 decreased cell death in pancreatic tissues from mice with AP (Fig. 3E).
Rg3 decreases inflammation and oxidative stress in mice with AP
The levels of inflammatory factors, including TNFα, IL6 and IL-1β, were measured in mice with AP treated with Rg3. RT-qPCR analysis demonstrated that Cn significantly increased then mRNA levels of TNFα, IL-6 and IL-1β in pancreatic tissues in comparison to those in the control mice. Treatment with Rg3 significantly reduced the TNFα, IL-6 and IL-1β mRNA levels in mice with AP (TNFα: 1±0.48 vs. 5.07±1.51 vs. 1.83±1.13 vs. 0.97±0.95; IL-6: 1±0.48 vs. 5.17±1.43 vs. 1.67±0.58 vs. 1.04±0.31; and IL-1β: 1±0.47 vs. 7.80±1.82 vs. 1.18±0.38 vs. 0.80±0.76 for the control, AP, AP + L-Rg3 and AP + H-Rg3, respectively) (Fig. 4A-C). Moreover, in contrast to the control mice, the AP-induced increase in the hs-CRP content was significantly reversed by treatment with high and low doses of Rg3 (12.3±5.64 vs. 146.85±57.91 vs. 43.69±16.26 vs. 25.68±9.57 mg/l for the control, AP, AP + L-Rg3 and AP + H-Rg3, respectively) (Fig. 4D). Furthermore, the MDA, ROS and Fe2+ contents were significantly increased in mice with AP; however, treatment with Rg3 reduced the MDA, ROS and Fe2+ levels in the pancreatic tissues of mice with AP (MDA: 1±0.09 vs. 1.70±0.12 vs. 1.19±0.056 vs. 1.03±0.07; ROS: 1±0.09 vs. 1.82±0.04 vs. 1.29±0.05 vs. 1.00±0.07; Fe2+: 1±0.05 vs. 0.58±0.06 vs. 0.71±0.03 vs. 0.80±0.02 for the control, AP, AP + L-Rg3 and AP + H-Rg3, respectively) (Fig. 4E-G). In comparison, the AP-induced reduction in GSH levels was markedly increased by Rg3 treatment in mice with AP (1±0.03 vs. 1.88±0.11 vs. 1.22±0.07 vs. 0.92±0.10 for the control, AP, AP + L-Rg3 and AP + H-Rg3, respectively) (Fig. 4H). These observations indicated a protective role for Rg3 in the pancreatic tissues of mice with AP.
Rg3 activates NRF2 signaling in pancreatic tissues from mice with AP
The transcription factor, NRF2, has been suggested to play a crucial role in AP-induced oxidative stress, and is also an important regulator of ferroptosis-related cell death (25,26). Hence, the effects of Rg3 on the NRF2-related ferroptosis pathway were examined in the present study. Western blot analysis revealed significantly reduced NRF2, HO-1, GPX4 and xCT levels in pancreatic tissues from mice with AP (Fig. 5A; each band represents tissue from each mouse in the specific group). By contrast, Rg3 treatment significantly increased the NRF2, HO-1, GPX4 and xCT expression levels (Fig. 5B; each band represents tissue from each mouse in the specific group). These observations indicated that the activation of NRF2 signaling may be a major contributor to the Rg3-mediated amelioration of AP injury.
Discussion
AP is a multifactorial disease, closely related to an excessive inflammatory response (27). The occurring type of cell death has been closely associated with the severity of AP (27). Ferroptosis is an iron-dependent oxidative programmed cell death pathway that is induced by lipid peroxidation (28). Based on accumulating evidence, treatment approaches targeting ferroptosis may have immense potential for use in the treatment of various diseases, including cancer and inflammation (29,30). However, the association between AP and ferroptosis remains unclear.
Ginsenoside Rg3 has been reported to be characterized by immunological adjuvant activity in various diseases (31,32). For instance, Rg3 has been reported to function as an anti-inflammatory agent by suppressing NF-κB activity and decreasing the NF-κB-mediated secretion of cytokines in A549 cells (32). In the present study, the viability of AR42J cells in the Rg3-treated group was increased compared with the group treated with Cn alone. Moreover, the number of dead AR42J cells was decreased in the Rg3-treated group compared with that in the group treated with Cn alone. These observations indicated a protective role for Rg3 in the Cn-induced experimental model of AP in vitro.
Oxidative stress is a major contributor to the severity of AP (33). ROS production may directly lead to an inflammatory response and recruit more oxidative stress-induced neutrophils to aggravate local tissue destruction, thereby promoting distant organ injury (34). Furthermore, ROS accumulation has been also suggested to be an apoptosis/necrosis switch in the development of AP (35). In the present study, a higher ROS level was observed in the Cn-treated group; however, Rg3 decreased ROS levels in vitro, indicating that Rg3 may exert an antioxidant effect to reduce ROS production. ROS-induced lipid peroxidation is a crucial contributor to cell death, including ferroptosis (36). Ferroptosis is induced upon the stimulation of elevated lipid ROS production, increased intracellular iron concentrations, and reduced levels of the antioxidant, GSH (37). In the present study, following treatment with Rg3, the production of MDA and Fe2+ in AR42J cells was reduced, accompanied by increased GSH levels in vitro. Furthermore, the decrease in GPX4 and xCT levels induced by Cn was reversed by treatment of the AR42J cells with Rg3. Thus, Rg3 may protect AR42J cells from ferroptosis by decreasing intracellular lipid ROS accumulation.
A crucial role for oxidative stress has been identified in the progression of AP, and targeting oxidative stress-related cell injury may be valuable for AP therapy (38). In the present study, mice administered Cn exhibited evident inflammatory damage and oxidative stress, as evidenced by increased TNFα, IL-6, IL-1β, pancreatic MDA, ROS and Fe2+ accumulation. In addition, Cn aggravated the severity of AP, as evidenced by increased cell death in pancreatic tissues. However, Rg3 treatment decreased ROS accumulation and cell death in pancreatic tissues. Furthermore, Rg3 suppressed Cn-induced ferroptosis in pancreatic tissues, due to a reduction in the cellular labile iron pool and increased GSH levels. Taken together, it was suggested that Rg3 may contribute to the amelioration of AP by suppressing ferroptosis.
NRF2 has been reported to play a crucial role in mediating lipid peroxidation and ferroptosis (25). NRF2 knockdown has been reported to noticeably reduce levels of the xCT and HO-1 proteins in a model of acute lung injury (39). The inhibition of NRF2 has also been demonstrated to reduce the antioxidant capacity and induce ferroptosis in a model of oxygen-glucose deprivation/reperfusion (OGD/R)-induced neuronal injury by suppressing GPX4 expression (40). In the model of Cn-induced AP, the decreased expression of components in the NRF2/HO-1 pathway has also been identified (41). In line with this finding, in the present study, Cn enhanced oxidative stress-induced injury and reduced the expression of NRF2/HO-1. In addition, levels of the xCT and GPX4 proteins were reduced in the pancreatic tissues of AP model mice. Following Rg3 administration, the NRF2/HO-1/xCT/GPX4 pathway was activated in pancreatic tissues.
In conclusion, the findings of the present study suggest, for the first time, to the best of our knowledge, a protective role for Rg3 in mice by suppressing oxidative stress-related ferroptosis and activating the NRF2/HO-1 pathway.