MCT4 expression in specimens of human BC and normal breast tissue was determined using tissue microarrays (cat. no. BC081116d; TissueArray.Com LLC, Rockville, MD, USA). Normal and breast cancer tissue were from different individuals. Slides were deparaffinized in xylene twice for 5 min each time, and rehydrated in 100 (twice), 95, 70 and 50% alcohol for 3 min each. Antigen retrieval was performed by heating in, 10 mM citric acid solution at 95°C for 15 min. The slides were blocked with 3% BSA (cat. no. 011-27055; FUJIFILM Wako Pure Chemical Corporation) for 30 min at room temperature. For immunohistochemical analysis, specimens were incubated with anti-MCT4 antibody (1:100; rabbit polyclonal; cat. no. sc-50329; Santa Cruz Biotechnology, Inc.) overnight at 4°C, followed by treatment with streptavidin-biotin complex (1:100; cat. no. K400311-2; EnVision System HRP-labeled polymer; Dako; Agilent Technologies, Inc.) for 60 min at room temperature and visualized with the use of a diaminobenzidine (DAB) substrate-chromogen solution (DakoCytomation Liquid DAB Substrate Chromogen System; Dako; Agilent Technologies, Inc.). Counterstaining with hematoxylin solution at room temperature for 10 sec was performed. Immunohistochemical staining positive area (%) was determined by calculating the positive area/total area ratio. Images were captured and quantification was performed using a hybrid cell count system (light microscope mode of BZ-X800 multiple analyzer BZ-H3A; Keyence Corporation) and ImageJ (Fiji) software (National Institutes of Health).

Ethics approval was not required for this research involving the use of human tissue microarray, due to the fact that this particular research fits into Section C, Paragraph 1, Part 3, Chapter 1 of Ethical Guidelines for Medical and Biological Research Involving Human Subjects issued by the Ministry of Education, Culture, Sports, Science and Technology, Japan. A waiver of ethics approval was provided by the Okayama University Ethics Committee (Okayama, Japan).

Puromycin dihydrochloride (cat. no. sc-108071), control short hairpin (sh)RNA plasmid-A (cat. no. sc-108060), MCT4 shRNA lentivirus particles (mouse, cat. no. sc-40120-V; human, cat. no. sc-45892-V), anti-MCT4 antibody (rabbit polyclonal; cat. no. sc-50329) and anti-MCT1 antibody (mouse monoclonal; cat. no. sc-365501) were purchased from Santa Cruz Biotechnology, Inc. Anti-phosphorylated (p)-p44/42 MAPK antibody (pERK1/2; rabbit monoclonal; cat. no. 4370), anti-p44/42 MAPK antibody (ERK1/2; rabbit monoclonal; cat. no. 4695), anti-p-cAMP response element binding protein (pCREB) antibody (rabbit monoclonal; cat. no. 9198), anti-CREB antibody (rabbit monoclonal; cat. no. 9197), HRP-conjugated IgG antibody (goat anti-rabbit; cat. no. 7074), HRP-conjugated IgG antibody (goat anti-mouse; cat. no. 7076) and Alexa Fluor 488-conjugated IgG (H+L) F(ab')₂ fragment (goat anti-rabbit; cat. no. 4412) were purchased from Cell Signaling Technology, Inc. Anti-calcitonin gene-related peptide (CGRP) antibody (goat polyclonal; cat. no. ab36001) and Alexa Fluor 647-conjugated IgG H&L (donkey anti-goat; cat. no. ab150135) were purchased from Abcam. Anti-GPR81 antibody (rabbit polyclonal; cat. no. NLS2095) was purchased from Novus Biologicals, LLC. Anti-β-actin antibody (rabbit polyclonal; cat. no. ab8227) was purchased from Abcam and used as loading control.

The human BC cell lines MDA-MB-231 and MCF-7 and mouse BC cell line 4T1 were obtained from the Japanese Collection of Research Bioresources Cell Bank. Mouse BC cell line EO771 was purchased from CH3 BioSystems. Rat dorsal root ganglion (DRG) cell line F11 (a mouse N18TG2 neuroblastoma and rat DRG sensory neuron hybrid cell line) (16) has been maintained in TY's laboratory obtained from American Type Culture Collection (17). All cells were cultured in DMEM (Thermo Fisher Scientific, Inc.) supplemented with 10% heat-inactivated FBS (Biosera) and 1% penicillin-streptomycin in an atmosphere of 5% CO₂ at 37°C. All cell lines were analyzed and authenticated by targeted genomic and RNA sequencing.

BC cells were plated at 1×10⁵ cells/well (6-well plate), cultured for 48 h and the cell number was counted using a TC20 automated cell counter (Bio-Rad Laboratories, Inc.).

EO771 cells (1×10⁵; 96-well plate) were cultured for 48 h in the presence of Adriamycin (cat. no. HY-15142; MedChemExpress) (30 nmol/l) to prevent cell proliferation, and pHe was measured using a FiveEasy pH meter (Mettler Toledo) immediately after cell removal from CO₂ incubator, as previously described (9).

The lactate concentrations of the bone marrow fluids of tibiae, BC cell (1×10⁵) culture supernatants cultured in 12-well plates for 48 h and cell lysates of sub-confluent sh-MCT4 or sh-control EO771 cells seeded in 12-well plates for 24 h, were measured using a lactate assay kit (cat. no. ECLC-100; EnzyChrom™; BioAssay Systems) according to the manufacturer's instructions.

Intracellular calcium changes were determined as previously described (9). In brief, 1×10³ F11 sensory neuron cells (seeded onto 35 mm glass bottom dishes) were exposed to Fura-2AM at 3 mM (Invitrogen; Thermo Fisher Scientific, Inc.) for 15 min at room temperature. Intracellular calcium changes were monitored in a balanced salt solution (BSS) composed of NaCl 140 mM, HEPES 10 mM, glucose 10 mM, KCl 5 mM, CaCl₂ 2 mM and MgCl2 1 mM. Following Fura-2AM exposure, the coverslips were rinsed a total of three times in BSS.

Changes in intracellular Ca²⁺ of F11 cells after treatment with EO771 conditioned medium (CM; 30% v/v) or 10 µM 3,5-dihydroxybenzoic acid (3,5-DHBA) (R&D Systems, Inc.) or 20 mM lactic acid (FUJIFILM Wako Pure Chemical Corporation) at room temperature were observed and recorded with digital video microfluorometry for 5 min, 90 sec and 150 sec, respectively, using an intensified CCD camera coupled to a Nikon Eclipse TE2000U microscope with Nikon Elements^® software (Nikon Instruments Inc.). With the use of a 150 Watt xenon arc lamp, the coverslips were illuminated, and the 340/380 nm excitation wavelengths of Fura-2AM were selected by a Lambda DG-5 plus illumination system (Sutter Instrument Company). Prior to the administration of CM, 3,5-DHBA or lactic acid, sterile BSS was applied, and any cells showing a response to buffer alone were excluded from data collection.

MDA-MB-231 and EO771 BC cells were infected with 1 µg control shRNA (cat. no. sc-108080-V; Santa Cruz Biotechnology, Inc.) or MCT4 shRNA using a lentiviral particle transduction system (multiplicity of infection, 2.0) (cat. nos. sc-45892-V and sc-40120-V; Santa Cruz Biotechnology, Inc.) in the presence of 5 µg/ml polybrene (sc-134220; Santa Cruz Biotechnology, Inc) for 24 h. Subsequently, infected cells were cultured in DMEM with 10% FBS for 7 days in the presence of 2 µg/ml puromycin to select cells stably expressing the shRNAs, following which subsequent experiments were performed. Santa Cruz Biotechnology shRNAs are provided as pools of 3 target specific 19-25 nucleotide molecules designed to knock down expression of specific genes of interest.

F11 cells were infected with 1 µg of control shRNA or GPR81 shRNA using a lentiviral particle transduction system (multiplicity of infection, 2.0) (cat. nos. sc-108080-V and sc-44644-V; Santa Cruz Biotechnology, Inc.) in the presence of 5 µg/ml polybrene for 24 h, and cultured in DMEM with 5% FBS for 7 days in the presence of 4 µg/ml puromycin to select cells stably expressing the shRNAs, following which subsequent experiments were performed.

F11 cells were cultured with or without sh-control or sh-MCT4 EO771 CM (30% v/v) at 37°C for 15min. sh-GPR81 F11 cells were cultured with or without EO771 CM (30% v/v) at 37°C for 15 min. Proteins from each cell line (MDA-MB-231, EO771, MCF-7, 4T1 and F11) were obtained after lysis with RIPA lysis buffer (cat. no. 9803; Cell Signaling Technology, Inc.) and protein concentrations were measured using BCA protein assay kit (cat. no. T9300A; Takara Bio, Inc.). A total of 10 µg of protein from the cell lysates were mixed with 4X Laemmli sample buffer (Bio-Rad Laboratories, Inc.) and heated at 95°C for 5 min. The samples were electrophoresed using 4-12% SDS-PAGE, and the proteins were transferred onto PVDF membranes. Membranes were blocked with 5% BSA for 60 min at room temperature. The membranes were incubated with the following antibodies: Primary antibodies against MCT4 (1:1,000), pERK1/2 (1:1,000), ERK1/2 (1:1,000), CREB (1:1,000), pCREB (1:1,000) and GPR81 (1:200) were incubated overnight at 4°C. Secondary HRP-conjugated anti-rabbit (1:2,000) and HRP-conjugated anti-mouse antibodies (1:2,000) were incubated for 60 min at room temperature. An ECL reagent (cat. No. RPN2109; Amersham; Cytiva) was used to detect secondary antibody binding. A ChemiDoc MP system (Bio-Rad Laboratories, Inc.) and Image Lab software v6.1 (Bio-Rad Laboratories, Inc.) were used for the analysis of blots. β-actin (1:2,000) antibody was used as a protein loading control.

All animal studies were approved by the Institutional Animal Care and Use Committee at the Indiana University School of Medicine (IACUC protocol no. 11170; Indianapolis, USA) and conducted according to the ARRIVE guidelines.

A mouse model of bone metastasis of human BC cells in 5-week-old female BALB/c athymic nude mice (n=20) and mouse BC cells in 5-week-old female C57BL/6 mice (n=20) was established (n=5/group; mean body weight, 19.5 g) (Envigo). Mice were maintained at temperature of 20°C, humidity of 50% with 12/12 h light/dark cycle and were provided with water and food ad libitum. Mice were injected with 1×10⁵ MDA-MB-231 human BC or EO771 mouse BC cells (parental, sh-control or sh-MCT4) using a 29-gauze needle into the bone marrow cavity of the right tibiae under anesthesia with xylazine (10 mg/kg) + ketamine (100 mg/kg) administered intraperitoneally. The sham mice received only PBS with a 29-gauze needle into the right tibial bone marrow cavity. Injection of parental, sh-control, sh-MCT4, MDA-MB-231 and EO771 cells were performed with a 29-gauze needle into the right tibial bone marrow cavity. Injection was performed by a single researcher who was blinded to the experimental conditions.

The bone destruction in tibiae associated with MDA-MB-231 and EO771 BC tumor progression was assessed by radiography. The bones harvested at sacrifice were placed against films (22×27 cm; Fuji industrial film; FUJIFILM Wako Pure Chemical Corporation) and exposed to soft X-rays at 35 kV for 15 sec with an X-ray device (CMB-2; Softex Co., Ltd.). The area of the radiolucent osteolytic lesions was quantified using Lumina Vision/OL v2.5 analysis software (MITANI Corporation) with a light microscope (IX81; Olympus Corporation) and ImageJ software as previously described (18).

Mice were individually placed in a cage with a glass floor, over a movable infrared light source. The light source was positioned under the center of the hind paw, and the time (sec) from stimulus onset to the paw's withdrawal was recorded. Mechanical allodynia was evaluated using von Frey tests and a Dynamic Plantar Aesthesiometer (Ugo Basile SRL) as previously described (19). These tests are widely used for pain assessment in rodents (9). The tests were performed prior to BC cell injection to determine baseline behaviors and then every 3 days following cell injection. Throughout the experiment, behavioral tests were performed by a single researcher who was blinded to the animal experimental conditions.

At day 15 after BC cell injection, 100 µl of blood was collected from each mouse through cardiac puncture under anesthesia with 4% isoflurane inhalation, followed by cervical dislocation. Animal death was verified by cessation of cardiovascular and respiratory movements. Ipsilateral lumbar DRGs (L3-L5) and the right tibia were then harvested for subsequent analyses. The criteria of humane endpoints for euthanasia included loss of >20% body weight compared with age-matched controls.

The collected mouse DRGs were homogenized in RIPA lysis buffer with 1 mM PMSF and phosphatase inhibitors (Na₃VO₄ and NaF). The lysate was centrifuged at 15,000 × g for 5 min at 4°C, and the supernatant was collected as total protein. Some of the collected DRGs were fixed for 12 h at room temperature with 10% neutral-buffered formalin and then embedded in paraffin. Western blotting and immunofluorescence were subsequently performed. For the collection of bone marrow fluids from tibias, the mouse muscles and connective tissues were removed, both ends of the tibias were cut, and whole bone marrow was flushed out with 100 µl PBS, followed by centrifugation (1,500 × g; 4°C; 10 min) and collection of the supernatants.

An immunofluorescence analysis was conducted to determine the expression of pERK1/2 and CGRP, a widely used marker for sensory neurons (20), in DRGs from each group of mice using an epifluorescence microscope (BZ-X810; Keyence Corporation). Formalin-fixed paraffin-embedded tissues (as aforementioned) were sectioned with 5-µm thickness. Slides were deparaffinized in xylene twice for 5 min each time, and rehydrated in 100 (twice), 95, 70 and 50% alcohol for 3 min each. Antigen retrieval was performed by heating in, 10 mM citric acid solution at 95°C for 5min. The specimens were incubated with 3% BSA in PBS blocking solution for 1 h at room temperature and then with primary pERK1/2 antibody (1:200) and anti-CGRP antibody (1:200) overnight at 4°C, followed by incubation with Alexa Fluor 488 anti-rabbit IgG (1:1,000) and Alexa Fluor 647 anti-goat IgG (1:1,000) for 60 min at room temperature. Nuclei were counterstained with Fluoroshield mounting medium with DAPI (cat. no. ab104139; Abcam).

Data are presented as the mean ± SD. The data were analyzed using an unpaired Student's t-test for comparisons between two groups or one-way ANOVA with Bonferroni's, Dunnett's or Tukey's post hoc tests for the analysis among multiple groups, using GraphPad Prism v7.0 software (GraphPad Software, Inc.). P<0.05 was considered to indicate a statistically significant difference.

It was first determined whether the lactate transporter MCT4 is expressed in BC tissues compared with normal breast tissues using immunohistochemistry. Representative histological images showed that MCT4 expression in BC tissues was more intense than that in normal breast tissues (Fig. 1A). Tissue microarray analysis revealed that the MCT4-positive area of BC samples was significantly increased compared with that in normal breast tissues (Fig. 1B). These results suggested that MCT4 expression is associated with BC tumor development.

Based on these data, the expression of MCT4 and MCT1 in human (MDA-MB-231 and MCF-7) and mouse BC cell lines (4T1 and EO771) was next determined by western blot analysis, to discover human and mouse BC cell lines that exhibited high MCT4 expression and thus were suitable for further experiments. MCT1 is also the lactate transporter that controls the intra- and extracellular levels of lactate (6). Since normal breast epithelial cells are currently unavailable and the data depicted in Fig. 1A demonstrated that normal breast tissues rarely expressed MCT4, control cells were not included in the western blot analysis. EO771 BC cells showed strong expression of MCT4 and MCT1 (Fig. 1C), MDA-MB-231 cells showed moderate expression of MCT4 and MCT1, while MCT4 expression was undetectable in human MCF-7 and mouse 4T1 BC cells. Of note, these BC cells released lactate into the culture supernatant in agreement with the degree of MCT4 and MCT1 expression (Fig. 1D), demonstrating that human and mouse BC cell lines express functional MCT4.

To determine the role of MCT4 in sensory neuron excitation and following BC-BP induction, MCT4 was silenced in mouse EO771 BC cells using a lentiviral system (sh-MCT4 EO771 BC cells). The expression of MCT4 protein was >80% decreased in the sh-MCT4 EO771 BC cells compared with the parental cells and sh-control cells (Fig. 2A). The expression of MCT4 in the sh-control EO771 cells did not differ from that of the parental EO771 cells. The sh-MCT4 EO771 BC cells were then injected into the bone marrow cavity of tibiae in the syngeneic female C57BL/6 mice, and the pain behavior of these mice was determined compared with the mice intratibially injected with parental or sh-control cells. No mice that received intratibial injection of mouse EO771 BC cells died until sacrifice at day 15. As previously reported using mouse 4T1 BC cells (21), the parental EO771 BC cells aggressively colonized the bone marrow cavity of tibiae accompanied with evident osteolysis (Fig. 2B, left), and sh-MCT4/EO771 BC cells also appeared to grow to an equivalent size to that of the parental EO771 BC cells in tibiae (Fig. 2B, right), suggesting that MCT4 silencing had no effects on EO771 BC growth in the bone. It is noteworthy that our previous study showed that the area of osteolysis detected on radiographs correlated well with the tumor size in bone determined by histomorphometry (18). Tumor growth could not be macroscopically observed, because the EO771 tumors grew in the bone marrow cavity. Furthermore, no tumor development was macroscopically detected at sites other than bone at day 15, although microscopic metastases could possibly develop in visceral organs including lung, liver and brain (18,21). There were no differences in body weight between mice injected with the EO771 parental, sh-control and sh-MCT4 EO771 cells at day 15 (Fig. S1A).

The excitation of the DRG neurons was evaluated by immunohistochemistry using pERK1/2 as a biochemical marker for neuron excitation (17). DRGs harvested from mice injected with parental or sh-control EO771 BC cells at sacrifice (day 15) demonstrated elevated expression of pERK1/2, while the DRG of mice injected with sh-MCT4 EO771 cells showed minimum pERK1/2 expression (Fig. 2C). Interestingly, the number of the CGRP-positive sensory neurons appeared unchanged among the DRGs of the three groups of mice (Fig. 2C).

Consistent with these in vivo results, the F11 DRG neuron cells showed increased expression of pERK1/2 and pCREB (a biochemical marker for neuron excitation) (22) in the presence of CM (30% v/v) harvested from the cultures of the sh-control EO771 BC cells (Fig. 2D). By contrast, the expression of pERK1/2 and pCREB was not upregulated in the F11 DRG neuron cells treated with the CM of sh-MCT4 EO771 BC cells (Fig. 2D). These results supported the hypothesis that MCT4-mediated lactate release from BC leads to the excitation of the DRG sensory neurons. These in vitro results were also consistent with the assumption there is sufficient time and chance for the nociceptive soluble tumor products that are released as a consequence of the interactions between GPR81 at the sensory endings and cancer cells to upregulate the expression of pERK1/2 and pCREB in the DRG.

It was then determined whether MCT4 plays a role in BC-BP induction using the pain behavior assay. Mice carrying the parental or sh-control EO771 BC cells began to exhibit decreased paw withdrawal threshold in the pain behavior assay using von Frey test at day 6 and 9 (Fig. 2E), demonstrating that these mice were developing mechanical allodynia. Mechanical allodynia advanced as a function of time in parallel with EO771 BC growth in the bone. By contrast, mice intratibially injected with sh-MCT4 EO771 BC cells showed reduced mechanical allodynia compared with mice carrying parental or sh-control EO771 BC cells (Fig. 2E). These results suggested that MCT4 plays an important role in BC-BP induction by mediating lactate release. Lactate acidosis may also contribute to the induction of BC-BP represented by mechanical allodynia by activating acid-sensing nociceptors, such as the transient receptor potential vanilloid 1 and acid-sensing ion channels (23).

The effects of MCT4 knockdown on lactate metabolism in EO771 mouse BC cells were subsequently investigated in vitro. Lactate secretion into the culture supernatants was significantly decreased (Fig. 3A) and pHe was significantly elevated (Fig. 3B) in sh-MCT4 EO771 cells compared with those in the parental and sh-control EO771 cells. These results suggested that MCT4 regulates lactate secretion and pHe in EO771 mouse BC cells.

In addition, the CM of parental and sh-control EO771 BC cell cultures also increased Ca²⁺ influx, a widely used indicator of sensory neuron excitation (24), in the F11 DRG neuron cells, while sh-MCT4 EO771 CM increased Ca²⁺ influx to a lesser extent than the CM of parental and sh-control EO771 BC cells (Fig. 3C). Consistent with the present results, the F11 cells are reported to display functional DRG neuron properties, including unique neuronal morphology of extended neurites and Ca²⁺ mobilization in response to stimuli (25) without the presence of glia cells. Furthermore, it was recently found that the F11 cells possess neuron-like properties, responding to the soluble products released from BC cells (data not shown). These data will be investigated in future studies. Taken together, these results suggested that lactate released from EO771 mouse BC cells via MCT4 excites sensory neuron cells.

The role of MCT4 expression in lactate metabolism and BP induction in human MDA-MB-231 BC cells was next investigated. To this end, MCT4 was knocked down in MDA-MB-231 cells by introducing shRNA plasmids using a lentiviral system, thereby generating sh-MCT4 MDA-MB-231 cells. The expression of MCT4 protein was >80% decreased in the sh-MCT4 MDA-MB-231 cells compared with the parental cells and sh-control cells (Fig. 4A). The expression of MCT4 in the sh-control MDA-MB-231 cells did not differ from that of the parental MDA-MB-231 cells.

Cell proliferation was not changed among parental, sh-control and sh-MCT4 MDA-MB-231 cells (Fig. 4B). Importantly, the intracellular lactate level was increased (Fig. 4C) and lactate secretion was reduced by ~40% (Fig. 4D) in sh-MCT4 MDA-MB-231 cells compared with those in the parental and sh-control MDA-MB-231 cells. These results suggested that MCT4 regulates intracellular and extracellular levels of lactate in human MDA-MB-231 BC cells.

Subsequently, the role of MCT4 in BC-BP after intratibial injection of MDA-MB-231 cells in Balb/c athymic nude mice was determined. Mice injected with MDA-MB-231 sh-control and sh-MCT4 cells showed bone destruction associated with tumor growth on the radiographs at day 7 (Fig. 5A). There was no significant difference in the extent of bone destruction between the MDA-MB-231 sh-control and sh-MCT4-injected mice (Fig. 5A and B).

To examine the effects of MCT4 expression on BC-BP induction in mice, a mechanical allodynia test was performed at day 10. Mice intratibially injected with parental and sh-control MDA-MB-231 BC cells exhibited mechanical allodynia (Fig. 5C). By contrast, mechanical allodynia was significantly decreased in mice injected with sh-MCT4 MDA-MB-231 cells compared with mice injected with parental and sh-control MDA-MB-231 BC cells (Fig. 5C), suggesting that MCT4 expression is critical to BC-BP induction.

The lactate concentration in the bone marrow fluid of tibiae injected with MDA-MB-231 human BC cells was also determined. Lactate concentration was elevated in the fluids harvested from the bone marrow of tibiae injected with sh-control MDA-MB-231 BC cells compared with that of sham-operated tibiae (Fig. 5D). By contrast, lactate concentration in the fluids harvested from the bone marrow of tibiae injected with sh-MCT4 MDA-MB-231 cells was not significantly increased compared with those injected with sh-control MDA-MB-231 cells (Fig. 5D). These results collectively suggested that inhibition of MCT4 expression decreased lactate release from BC cells into the bone marrow cavity, which in turn reduced BC-BP. There were no differences in body weight between mice injected with the MDA-MB-231 parental, sh-control and sh-MCT4 MDA-MB-231 cells at day 15 (Fig. S1B).

Finally, the mechanism via which BC-secreted lactate excites the sensory neurons to induce BC-BP was determined. GPR81 has been initially found as a lactate receptor that mediates lipolysis in the adipose tissue (14). It has been also shown that GPR81 regulates neuronal network activity in cooperation with other GPRs (26). However, little is known about the expression and function of GPR81 in sensory neurons. The present study found that F11 DRG neuron cells express GPR81 by western blot analysis (Fig. 6A). To determine the role of GPR81 in sensory neuron excitation, GPR81-knockdown F11 DRG neuron cells were established via shRNA transduction (sh-GPR81 F11 cells). GPR81 protein expression was reduced by 70% in sh-GPR81 F11 DRG neuron cells compared with parental and sh-control F11 cells (Fig. 6A). Of note, the CM harvested from EO771 BC cultures, which contains high levels of lactate, increased the expression of pERK1/2 in the F11 cells (Fig. 6B), which was almost abolished in the sh-GPR81 F11 cells. These results suggested that GPR81 contributes to sensory neuron excitation caused by BC-derived lactate.

Consistent with these results, treatment with the GPR81 agonist 3,5-DHBA (27) increased Ca²⁺ influx in parental and sh-control F11 cells (Fig. 6C), which was not observed in sh-GPR81 F11 cells (Fig. 6C). Furthermore, lactic acid treatment also increased Ca²⁺ influx in parental F11 cells (Fig. 6D), which was reduced in sh-GPR81 F11 cells. These results provided additional evidence supporting that GPR81 plays important roles in the excitation of sensory neurons by lactate.