Introduction
Breast cancer (BRCA) is the most common cancer in women worldwide. In total, >43,000 BRCA-related deaths are estimated in 2023 (1), and this may be due to a high degree of tumor heterogeneity. The histological subtypes of BRCA observed in clinical practice are Her2 (ER−, PR−, HER2+), LumA (ER+/PR+, HER2−), LumB (ER+/PR+, HER2+) and triple-negative BRCA (ER−, PR−, HER2−). Although a further understanding of BRCA subtypes has led to improved outcomes using targeted therapies, patients with triple-negative BRCA exhibit a poor prognosis due to augmented proliferative activity and acquired treatment resistance (2). Numerous drugs targeting the cell cycle have been developed to inhibit augmented proliferative activity, including gemcitabine, a chemotherapeutic agent for G1/S phase, and tyrosine kinase inhibitors, such as CDK4/6 (3). However, acquired treatment resistance to the aforementioned drugs was observed in patients following therapy (4,5). Notably, triple-negative BRCA may also be treated using anti-vascular endothelial growth factor (VEGF) therapy, including Avastin and Lucentis, which inhibit proliferative activity and angiogenesis. However, decreased tumor vessel and drug penetration, and increased hypoxia stimulated increased VEGF expression, resulting in resistance (6,7). Thus, the development of novel therapeutic targets is required to overcome the excessive proliferation and drug resistance of BRCA.
Long non-coding RNAs (lncRNAs), exhibit no coding potential and are >200 nucleotides in length. Notably, lncRNAs play a critical role in BRCA. For example, the upregulation of H19 inhibited the binding of DNA methyltransferase 3 β (DNMT3B) to the Beclin1 promoter region, resulting in tamoxifen resistance in BRCA cells. Moreover, H19 knockdown reversed this effect (8). In addition, LINC00511 promoted the proliferation of BRCA cells via sponging miR-185-3p to activate E2F transcription factor 1/Nanog signaling (9). Notably, lncRNA may be divided into numerous subgroups according to the location on the genome, including intronic, intergenic, divergent and antisense lncRNA. Thus, lncRNA and protein-coding transcripts may overlap in the genome but exhibit different functions. GATA binding protein 3 (GATA3) transcription activates Semaphorin 3B to inhibit BRCA development (10). By contrast, GATA3-AS1 destabilized the GATA3 protein, and enhanced the progression and immune escape of triple-negative BRCA through promoting GATA3 ubiquitination (11).
LOC127814295 [known as lnc-regulator of G protein signaling 5 (RGS5) or ENSG00000232995] is a novel lncRNA with a genomic region overlapping with protein-coding gene RGS5. As a protein-coding gene, RGS5 is involved in tumor development and tumor microenvironment remodeling. Results of a previous study demonstrated that the RGS5-TGFβ-PSmad2 axis reduces RGS5and TGFβ-dependent cell apoptosis through promoting PI3K-AKT signaling, and preventing mitochondrial damage and activation of caspases. This process leads to sustained pericyte survival and expansion in the tumor microenvironment (12). Notably, RGS5 promotes the occurrence of tumor angiogenesis in the tumor microenvironment of human melanoma and renal cancer xenografts (13). In addition, RGS5 exhibits potential as a widely expressed tumor antigen for identifying and characterizing T cell epitopes (14). However, studies focusing on the specific role of lnc-RGS5 in cancer are lacking, despite the deregulation of lnc-RGS5 in numerous cancer types. Therefore, the present study aimed to investigate the functional role of lnc-RGS5 and determine the clinical implications in BRCA. The present study also aimed to further elucidate the mechanistic role of lnc-RGS5 in regulating BRCA proliferation.
Materials and methods
Patient tissues and ethics approval
A total of 30 pairs of tissues from patients with BRCA were collected from The First Affiliated Hospital of Chongqing Medical University (Chongqing, China). Written informed consent was obtained from all patients. Women patients who were diagnosed with BRCA by two pathologists were included in the study (October 1, 2020 to December 30, 2020). All experimental procedures were approved by Ethics Committee of Chongqing Medical University (Chongqing, China) (approval date: September 20, 2020). Informed consent was obtained from all patients.
Analysis of gene expression and survival in public databases
Gene expression was analyzed in diverse cancer types detailed in The Cancer Genome Atlas (TCGA) database (https://portal.gdc.cancer.gov/). A comparison of gene expression between tumors and healthy samples was performed using the DESeq2 package (15) in R (version 4.2.3; https://www.r-project.org/). The expression correlation between gene pairs was performed using Pearson's correlation coefficient based on TCGA-BRCA dataset (https://portal.gdc.cancer.gov/projects/TCGA-BRCA). Benjamini-Hochberg adjusted P<0.05 was used as a threshold. Kaplan-Meier survival analysis was performed for triple-negative BRCA based on TCGA-BRCA dataset using R software (v4.2.3). Log-rank test was used for survival analysis and P<0.05 was used as a threshold. Median as cut-off was used.
Gene set enrichment analysis (GSEA)
The Gene Ontology (GO) functions and Kyoto Encyclopedia of Genes and Genomes pathways of lnc-RGS5 were analyzed using GSEA software (16). Patients were divided into high and low lnc-RGS5 expression groups according to the median expression of lnc-RGS5. Nominal P<0.05 was used as a threshold.
Regulation network analysis
A regulation network was constructed based on the putative interactions of genes. lnc-RGS5 and miRNA interactions, and miRNA-coding gene interactions were determined based on RNAhybrid (17) and miRanda (18) software. Notably, genes that interacted with miRNAs are often negatively correlated with miRNAs. Only genes differentially expressed in BRCA were considered. For transcription factors, putative targets were obtained from the Gene Transcription Regulation Database (GTRD) database (19).
Cell lines and culture
MCF-7 and MDA-MB-231 cell lines were purchased from the American Type Culture Collection (ATCC), and cultured in complete DMEM (Gibco; Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (FBS; Shanghai ExCell Biology, Inc.), 100 U/ml penicillin and 0.1 mg/ml streptomycin (Beyotime Institute of Biotechnology) at 37°C in a humidified atmosphere containing 5% CO2.
Cell transfection
Small interfering RNA (siRNA) targeting lnc-RGS5 and the negative control (NC) were purchased from Shanghai GenePharma Co., Ltd. Micro (mi)RNA inhibitor and mimics were purchased from Tsingke Biological Technology. Following the manufacturer's instructions, cells were transfected using siRNA-Mate (Shanghai GenePharma Co., Ltd.). The pcDNA3.1/forkhead box M1 (FoxM1) and pcDNA3.1/lnc-RGS5 plasmids were purchased from Tsingke Biological Technology and cells were transfected using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.). Cells were transfected with siRNAs (1 μg) or plasmids (2 μg) and cultured at 37°C for 48 h to perform subsequent experiments. Sequences were as follows: silnc-RGS5-1, 5′-UUU AAA GUG CAG UCU CUG UAC-3′; silnc-RGS5-2, 5′-UUU AAU GCC AUC CUG GCC AGA-3′; silnc-RGS5-3, 5′-UUU AAA CAG GUG AUC CCU AGA-3′; siFoxM1, 5′-GGA CCA CUU UCC CUA CUU U-3′; siNC, 5′-UUC UCC GAA CGU GUC ACG UTT-3′; miR-542-5p inhibitor, 5′-UCU CGU GAC AUG AUG AUC CCC GA-3′; NC inhibitor, 5′-UCU ACU CUU UCU AGG AGG UUG UGA-3′; miR-542-5p mimics, 5′-UCG UGA CAU GAU GAU CCC CGA UU-3′; and NC mimics, 5′-UCA CAA CCU CCU AGA AAG AGU AGA-3′.
RNA extraction and reverse transcription-quantitative (RT-q) PCR
Total RNA was extracted from MCF-7 and MDA-MB-231 cells using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. RNA (500 mg) was reverse transcribed into a 10 μl final volume of cDNA using Reverse Transcription kit (Takara Bio, Inc.), following the manufacturer's instructions. lnc-RGS5 and miR-542-5p RNA expression were measured using qPCR on the Step One Plus Real-Time PCR system. qPCR was conducted in three independent experiments using SYBR® Premix Ex Taq™ II (Takara Bio, Inc.) and analyzed using the 2−ΔΔCq method (20). qPCR reaction conditions were as follows: Initial denaturation at 95°C for 30 sec, followed by 39 cycles at 95°C for 5 sec and 60°C for 30 sec. GAPDH and U6 were used as endogenous controls. The primers were designed by Tsingke Biological Technology. qPCR primer sequences were as follows: GAPDH forward, 5′-CCA TGG GGA AGG TGA AGG TC-3′ and reverse, 5′-AGT GAT GGC ATG GAC TGT GG-3′; U6 forward, 5′-GCT TCG GCA GCA CAT ATA CTA AAA T-3′ and reverse, 5′-CGC TTC ACG AAT TTG CGT GTC AT-3′; lnc-RGS5 forward, 5′-AGT GAC AAG ATG GGG GTG TTC-3′ and reverse, 5′-CTG GTG GCT TCT GTT GGT TTG-3′; miR-542-5p forward, 5′-TCG GGG ATC ATC ATG T-3′ and reverse, 5′-GTG CAG GGT CCG AGG T-3′; and miR-542-5p, 5′-GTC GTA TCC AGT GCA GGG TCC GAG GTA TTC GCA CTG GAT ACG ACC TGC GGT CTC GTG-3′.
Western blot analysis
MCF-7 and MDA-MB-231 cells were transfected with miRNA and siRNA and cultured for 48 h. Total protein was extracted from cells using lysis buffer (cat. no. KGP250; Keygene Biotech, Inc.; http://www.keygentec.com.cn/), and the protein concentration was measured using Enhanced BCA Protein Assay Kit (cat. no. P0010; Beyotime Institute of Biotechnology). A total of 40 μg proteins were loaded per lane in SDS-PAGE. Proteins were separated via SDS PAGE on a 10% gel. The separated proteins were subsequently transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat milk in TBST (1 ml/l Tween-20; cat. no. ST825; Beyotime Institute of Biotechnology) at room temperature (RT) for 1 h, and incubated with the following primary antibodies: Anti-β-actin (1:1,000; cat. no. 20536-1-AP; ProteinTech Group, Inc.), anti-FoxM1 (1:1,000; cat. no. ab207298; Abcam), anti-VEGFA (1:1,000; cat. no. ab214424; Abcam) and anti-Neuropilin 1 (NRP1; 1:1,000; cat. no. ab81321; Abcam) overnight at 4°C. Following primary incubation, membranes were incubated with the HRP-conjugated secondary antibody (1:5,000; cat. no. SA00001-2; ProteinTech Group, Inc.) at RT for 1 h. The protein ladder was purchased from Shanghai Epizyme Biomedical Technology Co., Ltd. (cat. no. WJ103; 10~250 kDa). Protein bands were visualized using the Pierce ECL Plus Western Blotting Substrate (Bio-Rad Laboratories, Inc.). Protein expression was quantified using ImageJ 5.2.1 software (National Institutes of Health) with β-actin as the loading control.
RNA immunoprecipitation assay
RNA immunoprecipitation assays were performed using a BersinBio™ RIP kit according to the manufacturer's instructions. The MCF-7 and MDA-MB-231 cells (density: ~90%) were lysed using lysis buffer containing a protease inhibitor cocktail and RNase inhibitor, and the lysates were immunoprecipitated with anti-Ago2 (5 μl; cat. no. 67934-1-Ig; ProteinTech Group, Inc.) and IgG (5 μl; cat. no. 30000-0-AP; ProteinTech Group, Inc.) antibodies at 4°C overnight. A total of 30 μl Protein A-Agarose beads (cat. no. sc-2001; Santa Cruz Biotechnology, Inc.) were added and incubated at 4°C for 2 h. After centrifugation at 200 x g for 30 sec, beads were 3 times washed with wash buffer (20 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5% Triton X-100, 0.4 U/μl RNase inhibitor, and 0.4 U/μl Protease Inhibitor Cocktail). The retrieved RNAs were quantified using RT-qPCR.
Dual-luciferase reporter assay
Transfection and luciferase reporter assays were performed as previously described (21). lnc-RGS5 cDNA containing the predictive binding site of miR-542-5p was cloned into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega Corporation) to form the wild-type vector (lnc-RGS5-WT). Mutant (Mut) lnc-RGS5 containing mutations of the miR-542-5p binding site was specifically synthesized and inserted into the aforementioned vector (lnc-RGS5-Mut). BRCA cells were cultured and co-transfected with pmirGLO-lnc-RGS5-3′-untranslated region (UTR) vectors, including WT or Mut fragments and miR-542-5p mimics. The pmirGLO vector was used as the NC. FoxM1-WT and FoxM1-Mut were cloned into the pmirGLO vector (Promega Corporation) using the one-step directed cloning kit (Novoprotein Scientific, Inc.). miR-542-5p mimics were co-transfected with FoxM1-WT or FoxM1-Mut vector into BRCA cells using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.). Luciferase activity was evaluated using Dual-Luciferase® Reporter Assay System (Promega Corp.) after 48-h transfection. Data were presented as a ratio of Firefly to Renilla luciferase activity.
Short hairpin (sh)RNA transfection
shRNA targeting lnc-RGS5 (shlnc-RGS5 sequence, 5′-GCA TGG TTG GAG ACA ATA AGT CTC GAG ACT TAT TGT CTC CAA CCA TGC-3′; target sequence, 5′-GCA TGG TTG GAG ACA ATA AGT-3′; 1 μg) was expressed using the pLKO.1-TRC-copGFP-T2A-Puro vector (TsingKe Biological Technology). A scrambled shRNA (5′-TTC TCC GAA CGT GTC ACG T-3′; 1 μg) was used as a negative control (shNC). MDA-MB-231 cells expressing green fluorescent protein were screened after 72 h transfection at 37°C. HiTransG P transfection agent (Shanghai Genechem Co., Ltd.) was used. To generate stable lnc-RGS5-knockdown cells, 2 μg/ml puromycin was used for induction, and 1 μg/ml puromycin was used for maintenance.
Cell viability and proliferation assay
A Cell Counting Kit-8 (CCK-8) assay (cat. no. 40203ES60; Shanghai Yeasen Biotechnology Co., Ltd.) was used to measure cell proliferation. In total, 2,000 cells were seeded in 96-well plates, and 10 μl CCK-8 solution was diluted and added. After incubation with CCK-8 reagent at 37°C for 1 h in dark, absorbance was measured at 450 nm. Cell proliferation was also detected using a BeyoClickTM EdU Cell proliferation kit according to the manufacturer's instructions (Beyotime Institute of Biotechnology). Cells were stained with Alexa Fluor 488 at RT for 30 min in dark, and observed using a fluorescence microscope using a 10X objective lens (magnification, ×100; Nikon Eclipse Ts2R; Nikon Corporation).
RNA expression in the nucleus and cytoplasm
RNA was extracted from the nuclei and cytoplasm using the PARIS kit (Thermo Fisher Scientific, Inc.), according to the manufacturer's instructions. An RNase inhibitor was used in RNA extraction. The purity of extracted RNA was evaluated, and high-quality RNA (260/280 nm ratio >1.8) was used for subsequent RT-qPCR experiments. U2 and β-actin were used as endogenous controls, and the primer sequences were as follows: U2 forward, 5′-CCT TTT GGC TAA GAT CAA GTG TAG TAT CTG TT-3′ and reverse, 5′-AGC AAG CTC CTA TTC CAT CTC CCT G-3′; and β-actin forward, 5′-CCT TCC TGG GCA TGG AGT C-3′ and reverse, 5′-TGA TCT TCA TTG TGC TGG GTG-3′.
In vivo tumor formation assay
Nude BALB/c-nu mice (age, 4 weeks; sex, female; weight, 16-18 g; n=16) were purchased from Huafukang Biotechnology Company. The in vivo tumor formation assay was performed as previously described (22). Briefly, 6×105 MDA-MB-231 cells resuspended using phosphate buffered saline (PBS) stably transfected with LV-shlnc-RGS5 or LV-NC were inoculated subcutaneously into the axillary fossa. Antagomir-542-5p and antagomir-NC (5 nmol/mouse each time), shFoxM1 (target sequence, 5′-CTCTTCTCCCTCAGATATA-3′), or shNC plasmids (10 μg/mouse each time) were injected into the tumors every three days following tumor formation (n=4 in each group, randomly allocated). The diameter of the largest tumor observed did not exceed 1.5 cm. At the end of the experiment, all mice were sacrificed by cervical dislocation. All animal experiments were blinded and carried out in the IVC Laboratory under specific pathogen-free (SPF) conditions (4 nude mice per cage) of Barrier Facilities of Chongqing Medical University. All mice were group housed on a 12-h dark/12-h light cycle at temperatures of 18-23°C with 40-60% humidity, and were provided with sufficient food and water. All animal experiments were reviewed and approved (approval no. IACUC-CQMU-2022-0016) by the Experimental Animal Management and Use Committee of Chongqing Medical University (Chongqing, China).
Statistical analysis
Bioinformatics analysis was conducted using R software (version 4.2.3; https://www.r-project.org/). Statistical analysis for experiment results was conducted based on three replications using Prism 8 (Dotmatics). Unpaired t-tests were used for two-group comparisons. A P<0.05 was considered to indicate a statistically significant difference. Error bars (mean ± standard deviation) were shown.
Results
Expression and clinical analyses of lnc-RGS5 in BRCA
In Fig. 1A, the lnc-RGS5 (LOC127814295) gene located on 1q23.3 is demonstrated. As a lncRNA, the transcripts of lnc-RGS5 were >200 nucleotides in length, with very low protein-coding potential (The Coding Potential Assessment Tool; CPAT software; 1.97%), no open reading frames and no translation initiation site (Fig. 1A). Results of TCGA data analysis demonstrated that lnc-RGS5 was upregulated in diverse cancer types, such as bladder cancer, esophagus cancer and BRCA (Fig. 1B). Moreover, lnc-RGS5 expression was significantly higher in basal-like or triple-negative BRCA than in Her2 (P=0.0006), LumA (P=0.028) or LumB (P=0.048) subtypes (Fig. 1C). However, lnc-RGS5 demonstrated no significant association with lymph node or distant metastasis (P>0.05; Fig. 1D). Results of the survival analysis demonstrated that high lnc-RGS5 expression was associated with poor overall survival of patients with triple-negative BRCA (cut-off, median lnc-RGS5 expression; P=0.045; Fig. 1E) and progression-free survival (cut-off, median lnc-RGS5 expression; P=0.018; Fig. 1F) of patients with BRCA. Results of the multivariate analysis demonstrated that lnc-RGS5 may act as an independent factor for progression-free survival (Fig. 1G). These results implied that lnc-RGS5 may play a critical role in the tumorigenesis of BRCA and may exhibit potential as a prognostic biomarker in triple-negative BRCA.
lnc-RGS5 is upregulated and increases cell proliferation in BRCA
Results of the RT-qPCR analyses indicated that lnc-RGS5 was upregulated in BRCA compared with healthy samples (Fig. 2A, Table I). GSEA demonstrated that high lnc-RGS5 expression was associated with increased activities of DNA repair, protein export and DNA replication. By contrast, low lnc-RGS5 expression was associated with decreased activities of mTOR, MAPK, Erb-B2 Receptor Tyrosine Kinase (ERBB) and VEGF signaling pathways (Fig. 2B). Collectively, these results suggested that lnc-RGS5 may be involved in sustaining the proliferative signaling of BRCA. Thus, the role of lnc-RGS5 was further validated in BRCA cell proliferation.
In the present study, lnc-RGS5 was overexpressed and silenced following transfection with lnc-RGS5 overexpression plasmid and lnc-RGS5 siRNAs, respectively (Fig. 2C). Results of the CCK-8 and EdU assays indicated that overexpression of lnc-RGS5 promoted proliferation, while si-lnc-RGS5 transfection inhibited the growth of BRCA cells (Fig. 2D-F). These findings confirmed that lnc-RGS5 significantly enhances the growth of BRCA cells in vitro.
Lnc-RGS5 functions as a competing endogenous RNA (ceRNA) for miR-542-5p in BRCA
To investigate the mechanism of lnc-RGS5 in BRCA, GO enrichment analysis was performed. Results of the present study demonstrated that lnc-RGS5 was associated with RNA binding involved in post-transcriptional gene silencing (Fig. 3A), such as ceRNA mechanisms. Results of the RT-qPCR analysis revealed that lnc-RGS5 was mainly expressed in the cytoplasm (Fig. 3B). Subsequently, an RNA immunoprecipitation assay was performed to determine whether Ago2, a key component of the RNA-induced silencing complex, may combine with lnc-RGS5 and further mediate the binding of miRNAs with lnc-RGS5. Results of the present study demonstrated the significant enrichment of lnc-RGS5 immunoprecipitated by the anti-Ago2 antibody, compared with anti-IgG (Fig. 3C). Collectively, results of the present study indicated that lnc-RGS5 may function as a ceRNA.
A ceRNA regulation network of lnc-RGS5 was subsequently constructed based on an integrative analysis (Fig. 3D). Results of the present study demonstrated that miR-542-5p (degree; 26) was the potential target of lnc-RGS5. VEGFA was the hub gene (degree; 15) of the downstream network regulated by miR-542-5p/FoxM1 signaling. NRP1 was one of the receptors of ligand VEGFA. Moreover, expression correlation analysis revealed that miR-542-5p was negatively correlated with lnc-RGS5 and FoxM1, while lnc-RGS5 was positively correlated with FoxM1 in BRCA (P<0.05; Fig. 3E). In addition, miR-542-5p was downregulated and FoxM1 was upregulated in BRCA, compared with healthy samples (Fig. 3F). These results were consistent with those demonstrating the ceRNA mechanism.
Subsequently, the binding sites of miR-542-5p on lnc-RGS5 and FoxM1 were mutated (Fig. 4A), and miR-542-5p was overexpressed following transfection with the miRNA mimics (Fig. 4B). Results of the dual-luciferase assay indicated that miR-542-5p significantly reduced the luciferase activity of lnc-RGS5 and FoxM1 in BRCA cells. However, no significant differences in the luciferase activity of lnc-RGS5-Mut and FoxM1-Mut were observed following miR-542-5p overexpression (Fig. 4C). Notably, transfection with the lnc-RGS5 overexpression vector reversed the decreased pmirGLO-FoxM1 3′UTR luciferase activity induced by miR-542-5p; however, this was not observed with the lnc-RGS5-Mut in which the miR-542-5p binding site was mutated (Fig. 4D). Collectively, these results demonstrated that miR-542-5p directly binds to lnc-RGS5 and FoxM1.
lnc-RGS5 promotes BRCA cell proliferation through the miR-542-5p/FoxM1 axis in vitro
Results of the RT-q-PCR analysis revealed that miR-542-5p was upregulated in silnc-RGS5 cells compared with siNC cells, while miR-542-5p was downregulated in cells overexpressing lnc-RGS5, compared with those transfected with the empty vector (Fig. 4E). Transfection with the lnc-RGS5 overexpression vector or miR-542-5p inhibitor upregulated the expression of FoxM1 and VEGFA/NRP1, while transfection with si-lnc-RGS5 or miR-542-5p mimics downregulated the corresponding expression (Fig. 4F and G). Moreover, FoxM1 overexpression promoted the expression of VEGFA/NRP1, while transfection with siFoxM1 inhibited the corresponding expression (Fig. 4H). Subsequently, the efficiency of shlnc-RGS5 and shFoxM1 transfection was determined (Fig. 5A and B). The decreased proliferative ability and FoxM1/VEGFA/NRP1 expression induced by shlnc-RGS5 were regained following co-transfection with the miR-542-5p inhibitor. Notably, these results were inhibited following transfection with shFoxM1 in BRCA cells (Fig. 5C-E). The decreased proliferative ability and protein expression levels of FoxM1/VEGFA/NRP1 induced by miR-542-5p mimics were regained following co-transfection with lnc-RGS5. Notably, these results were inhibited following transfection with shFoxM1 in BRCA cells (Fig. 6A-C). Thus, lnc-RGS5 competitively sponges miR-542-5p to prevent miR-542-5p binding to FoxM1 3′UTRs, resulting in FoxM1 upregulation and increased proliferation of BRCA cells.
lnc-RGS5 promotes BRCA cell proliferation through a ceRNA pattern in vivo
Analysis of BRCA tissues revealed that the expression of lnc-RGS5 was higher in patients with T3/T4 tumors compared with patients with T1/T2 tumors, while the expression of miR-542-5p was lower in patients with T3/T4 tumors compared with patients with T1/T2 tumors. Rescue experiments explored whether lnc-RGS5 exerts biological functions via a ceRNA pattern in vivo. Treatment with antagomir-542-5p significantly abolished the decreased tumor growth in LV-shlnc-RGS5 tumors, which was reversed following FoxM1 knockdown (Fig. 6E). Results of the western blot analysis of xenograft tumors demonstrated that treatment with antagomir-542-5p recovered the decreased protein expression of FoxM1/VEGFA/NRP1 in LV-shlnc-RGS5 tumors, and this was reduced following FoxM1 knockdown (Fig. 6F). Collectively, these data demonstrated that lnc-RGS5 acts as a ceRNA for miR-542-5p to promote BRCA cell proliferation in vitro and in vivo (Fig. 7).
Discussion
Results of the present study demonstrated that lnc-RGS5 was upregulated in BRCA tissues compared with healthy samples and associated with the overall survival of patients with triple-negative BRCA. Functionally, lnc-RGS5 promoted the proliferation of BRCA cells in vitro and in vivo. Mechanistically, lnc-RGS5 functions by competitively sponging miR-542-5p to promote FoxM1/VEGFA signaling. Thus, lnc-RGS5 may exhibit potential as a novel target for the treatment of BRCA.
Results of the present study revealed that lnc-RGS5 may act as a cancer-associated lncRNA. Pathway and functional analyses demonstrated that lnc-RGS5 was involved in DNA replication and signaling pathways associated with cell proliferation, differentiation and metastasis, such as mTOR, MAPK and VEGF signaling pathways. However, clinical data analysis revealed that the expression of lnc-RGS5 was not significantly associated with lymph node or distant metastasis. Thus, the present study focused on the regulatory role of lnc-RGS5 in the proliferation of BRCA cells. Notably, results of the GSEA revealed that RNA binding involved in post-transcriptional gene silencing was the most significantly enriched molecular function. Thus, a ceRNA network was constructed to investigate the potential mechanistic role of lnc-RGS5.
Results of the present study demonstrated that lnc-RGS5 knockdown inhibited FoxM1/VEGFA signaling via ceRNA mechanisms, implying that lnc-RGS5 may act as an alternative target for combined therapy with anti-VEGFA therapies, as anti-VEGFA monotherapy stimulated higher VEGF expression in BRCA (6,7). lnc-RGS5 may also be involved in DNA repair pathways, such as mismatch repair, base/nucleotide excision repair and homologous recombination. DNA damage is a hallmark of cancer as it may lead to tumor evolution and microenvironment remodeling, which is consistent with the functional results of the present study, in which lnc-RGS5 was associated with immune-associated pathways, such as FcγR-mediated phagocytosis. Further experiments are required to elucidate the molecular mechanisms underlying lnc-RGS5 in the tumor microenvironment.
FoxM1 transcription factor is a member of the forkhead box family. Results of a previous study revealed that FoxM1 upregulation promoted BRCA tumorigenesis (23), and VEGFA and NRP1 were also upregulated (24). However, the corresponding expression levels and roles in cell proliferation in BRCA were not further validated. FoxM1 is a critical downstream effector of PI3K-AKT and JNK/MAPK signaling pathways for cell proliferation, cell cycle control and DNA damage repair (25). Results of the present study revealed that FoxM1 may also be regulated by lnc-RGS5/miR-542-5p signaling to promote cell proliferation. FOXM1 is involved in three main cellular mechanisms of single-strand break repair; namely, nucleotide excision repair, base excision repair and mismatch repair (26). For example, FoxM1 transcriptionally promotes the expression of replication factor c subunit 5 to participate in nucleotide excision repair (27). Notably, results of the present study revealed the potential function of lnc-RGS5 in the regulation of DNA damage repair through regulating FoxM1. In addition, results of a previous study indicated that FoxM1 may exhibit potential as a target in the treatment of tumors (25). However, transcription factors are complex drug targets due to disordered structures and a lack of significant small molecule binding pockets (28). A previous study revealed an alternative approach to targeting transcription factors; for example, targeting the upstream non-coding RNA. Specifically, targeting lncRNA H19 inhibited FoxM1 in gallbladder cancer (29). Thus, lnc-RGS5 may act as a novel upstream target to suppress FoxM1 and VEGFA.
Results of a previous study revealed that the VEGFA/NRP1 axis may regulate cell proliferation in BRCA (30). Notably, autocrine VEGFA produced by tumor cells promoted tumor-forming capacity in vivo, independent of effects on angiogenesis through interaction with NRP1 (30). Considering the effect of VEGFA in angiogenesis with VEGF receptor-1/2 (31), VEGFA may promote cell growth through activating both VEGFR-1/2 and NRP1/Ras signaling pathways in BRCA. Future studies are required to determine whether lnc-RGS5 promotes BRCA cell growth via angiogenesis in vivo. Moreover, in future studies, more animals will be used for in vivo experiments.
As a tumor suppressor gene, miR-542-5p plays an important role in various tumors (32). miR-542-5p promotes the progression of BRCA through inhibiting Ubiquitin Specific Peptidase 17 Like Family Member 2 (USP17L2, also known as DUB3), and treatment with pristimerin reversed this process at the cellular level (32). Results of a previous study also demonstrated that miR-542-5p inhibits tumor progression in lung cancer through inhibiting EGFR (33). Thus, lnc-RGS5 may exhibit potential as a targeted therapeutic drug to supplement pristimerin, or as a target of combined therapy.
In conclusion, results of the present study revealed that lnc-RGS5 may act as a novel oncogenic lncRNA in BRCA, and may exhibit potential in the treatment of BRCA.