OL Oncology Letters 1792-1074 1792-1082 D.A. Spandidos 10.3892/ol.2024.14533 OL-28-2-14533 Articles Effects of α‑solanine on human head and neck squamous cell carcinoma cells and human umbilical vein endothelial cells in vitro Von FournierArmin KasemoTotta Ehret HackenbergStephan WilhelmChristian MeyerTill GehrkeThomas HagenRudolf ScherzadAgmal Department of Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University Hospital Wuerzburg, D-97080 Wuerzburg, Germany Correspondence to: Dr Armin Von Fournier, Department of Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University Hospital Wuerzburg, 11 Josef-Schneider-Strasse, D-97080 Wuerzburg, Germany, E-mail: fournier_a@ukw.de xiaojuanliu1979@163.com 08 2024 26 06 2024 28 2 400 31012024 09052024 Copyright: © 2024 Von Fournier et al. 2024 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

α-solanine is a glycoalkaloid that is commonly found in nightshades (Solanum) and has a toxic effect on the human organism. Among other things, it is already known to inhibit tumor cell proliferation and induce apoptosis in tumor cell lines. Due to its potential as a tumor therapeutic, the current study investigated the effect of α-solanine on head and neck squamous cell carcinoma (HNSCC). In addition, genotoxic and antiangiogenic effects on human umbilical vein endothelial cells (HUVECs) were evaluated at subtoxic α-solanine concentrations. Cytotoxicity and apoptosis rates were measured in two human HNSCC cell lines (FaDu pharynx carcinoma cells and CAL-33 tongue carcinoma cells), as well as in HUVECs. MTT and Annexin V analyses were performed 24 h after α-solanine treatment at increasing doses up to 30 µM to determine cytotoxic concentrations. Furthermore, genotoxicity at subtoxic concentrations of 1, 2, 4 and 6 µM in HUVECs was analyzed using single-cell gel electrophoresis (comet assay). The antiangiogenic effect on HUVECs was evaluated in the capillary tube formation assay. The MTT assay indicated an induction of concentration-dependent viability loss in FaDu and CAL-33 cancer cell lines, whereas the Annexin V test revealed α-solanine-induced cell death predominantly independent from apoptosis. In HUVECs, the cytotoxic effect occurred at lower concentrations. No genotoxicity or inhibition of angiogenesis were detected at subtoxic doses in HUVECs. In summary, α-solanine had a cytotoxic effect on both malignant and non-malignant cells, but this was only observed at higher concentrations in malignant cells. In contrast to existing data in the literature, tumor cell apoptosis was less evident than necrosis. The lack of genotoxicity and antiangiogenic effects in the subtoxic range in benign cells are promising, as this is favorable for potential therapeutic applications. In conclusion, however, the cytotoxicity in non-malignant cells remains a severe hindrance for the application of α-solanine as a therapeutic tumor agent in humans.

 α-solanine head and neck squamous cell carcinoma toxicology antitumor effects human umbilical vein endothelial cells drug safety Funding: Not applicable. Introduction

Glycoalkaloids are secondary metabolites from plants of the solanaceae family, which include widely consumed staple foods such as eggplant, tomato, and potato. There, they exert a natural protective function against fungi, bacteria, and predators (1). In recent years, there has been increasing interest in the possible antitumor activities of steroidal glycoalkaloids (2). Several publications can be found on the glycoalkaloid α-solanine (Molecular formula: C45H73NO15), which is found mainly in potatoes, where it accounts for up to 95% of the total glycoalkaloid content (3). Due to its known acute toxicity in mammals and thus also in humans, the Food and Agriculture Organization (FAO) of the United Nations (UN) and the World Health Organization (WHO) recommend a maximum daily dose of 200 mg kgG-1 (3). An additional concern is possible teratogenic effects, especially neural tube defects (NTDs). These have been demonstrated in various animal experiments, although the effect did not occur in a study with non-human primates (4). Regarding the antitumor effects of glycoalkaloids, especially α-solanine, a growing number of publications in recent years investigated these mechanisms in various tumor entities, including breast, lung, pancreas, liver, prostate, and skin (5,6). In terms of toxicity threshold and mechanisms of action, results and the conclusions drawn of them in literature showed a high variance. Activation of apoptosis via different pathways is often cited as a cause of tumor toxicity. α-solanine stimulated apoptosis-activating proteins in the liver carcinoma cell line HepG2 (ASK1: apoptosis signal-regulating kinase 1, TBP-2: tetrahymena piggyBac transposase 2) (7), in the colorectal carcinoma cell line RKO (Caspase-3) (8) and in the pancreatic carcinoma cell lines SW1990 and Panc-1 (each caspase-3) (9). In addition, influences on cell cycle regulation were investigated in the liver carcinoma cell line HepG2 (10).

Despite these studies raising hope for its potential usefulness as a tumor therapeutic agent, to our knowledge, there are no studies on the effect of α-solanine on human head and neck squamous cell carcinoma (HNSCC). HNSCC are a heterogeneous tumor group with more than 890.000 new cases and more than 450.000 deaths in the year 2018 alone (11). The exploration of potential new therapies is an urgent topic, as drug-based tumor therapies gain increasingly higher significance.

The aim of this study was to investigate the effect of α-solanine on HNSCC cells for the first time. Furthermore, the cytotoxicity as well as potential genotoxicity and functional impairment of a non-malignant cell line of Human Umbilical Vein Endothelial Cells (HUVECs) was evaluated at subtoxic doses.

 Materials and methods <sec> <title>Characterization of reagents and cell cultures

α-solanine (from potato sprouts, >95%) was purchased from Merck (Darmstadt, Germany). Two HNSCC derived cell lines were used. The first, established from a hypopharyngeal carcinoma (FaDu) (12), was cultured in MEM Eagle (Sigma) with penicillin/streptomycin (Sigma), FBS 10% (Anprotec) and Glutamin 1% (Sigma). The tongue carcinoma cell line (CAL-33; Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Gmbh) was cultured in DMEM (Gibco) with FBS 10% (Anprotec) and penicillin/streptomycin (Sigma). Cells were incubated at 37°C with 5% CO2 in 75 cm2 flasks (Greiner). The medium was replaced every second day. Cells were passaged by trypsinization (0.25% trypsin-EDTA (Gibco) (1×); Invitrogen (Gibco), Life Technologies, Karlsruhe, Germany) before reaching 80% of confluence. Then the cells were washed (1× centrifugation, 1,000 rpm, 5 min) and either seeded in treatment wells or in new 75 cm2 flasks. Non-malignant HUVECs (pooled donors, C12203, PromoCell GmbH, Heidelberg, Germany) were treated according to the procedures described in a previous study by our group (13). Briefly, the cells were cultured in an endothelial cell growth medium with supplements (ECGM; Provitro GmbH, Berlin, Germany) and in 1% penicillin/streptomycin. Mycoplasma testing has been carried out for the cell lines used.

 Cytotoxicity analysis in the MTT assay

Before the application of α-solanine, the number of FaDu, CAL-33, and HUVECs was determined using an electronic cell counter (Casy Technology, Innovatis AG, Reutlingen, Germany) and 1×104 cells per well were seeded in a 96-well plate (Greiner, 655180, flat bottom). After 24 h incubation, the medium was replaced with rpmI 1640 medium supplemented with α-solanine at concentrations of 3–30 µM for FaDu, 1–30 µM for CAL-33 and 1–18 µM for HUVECs. Previously, an adequate concentration range was determined from a wide panel of doses. Untreated negative controls were cultivated in rpmI 1640 only on the same cell culture plate.

After subsequent incubation for 24 h, cell viability was assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma Aldrich) colorimetric staining method (14). The expansion medium was removed and 100 µl of MTT solution (1 mg/ml dissolved in medium) was added to each well. This was followed by a 4 h incubation at 37°C with 5% CO2. MTT was then replaced with isopropanol, which dissolves the formed formazan crystals within 30 min at room temperature. Cells were kept dark. To remove all particles from the samples and avoid interference during measurements, the contents of each well were transferred to an Eppendorf tube, centrifuged (1,000 rpm, 5 min) and then transferred to a new 96-well plate without resuspending the pellet. Finally, the color conversion of the purple formazan dye was measured by an enzyme-linked immunosorbent assay reader at a wavelength of 570 nm. 1 mM tert-butyl hydroperoxide (t-BHP; Sigma-Aldrich, St. Louis, MO, USA) served as a positive control of metabolically inactive cells and medium without α-solanine was used as a negative control. All measurements were carried out in technical triplicates for FaDu, CAL-33 and HUVECs.

 Cell cycle analysis

For cell cycle analysis, FaDu were incubated with α-solanine at concentrations of 12, 15, 18 and 21 µM. After 24 h, the cells were fixed in the dark in 70% ethanol at 4°C for 2 h followed by centrifugation at 500 g for 5 min at 4°C. Then, 500 ml PI/RNase Staining Buffer (Becton-Dickinson Bioscience) was added followed by an incubation in the dark at 4°C for 15 min. Subsequently, flow cytometer (FACScanto, Becton-Dickinson) Measurements were performed.

 Flow cytometry

Time- and dose-dependent apoptosis and necrosis of tumor cells were measured by flow cytometry using an Annexin V propidium iodide (PI) kit (Becton-Dickinson Bioscience, Heidelberg, Germany) according to the manufacturer's protocol. Here, a distinction was made between viable cells, apoptotic cells (Annexin V), and necrotic or late-apoptotic cells (propidium iodide (PI)). FaDu and CAL-33 (2×10^5 (200,000)/well) were seeded in 6-well plates and incubated with α-solanine for 24 h. The experimental concentration range included concentrations of 12, 15, 18, 21, 24, 27 and 30 µM. After exposure, adherent cells were harvested by trypsinization as described above and added to the preserved medium. After two washing steps with centrifugation (500 g, 5 min, 4°C) and addition of phosphate buffer saline (PBS, Roche), the cell pellet was resuspended with 100 µl binding buffer (BD Pharmingen). For staining, 5 µl of Annexin V-APC and 5 µl of PI were added. After 15 min of incubation in the dark, the fluorescence of 1×104 cells per sample was measured by flow cytometry.

 Genotoxicity evaluation with the comet assay

For the detection of DNA strand breaks and alkali labile as well as incomplete excision repair sites in single cells, the comet-assay (alkaline version of the single-cell microgel electrophoresis) was used. Subcytotoxic doses of α-solanine in HUVECs were evaluated in the MTT assay as previously described. Doses of 1, 2, 4 and 6 µM were applied in the comet assay and a negative control was performed using culture medium. Additionally, 200 µM direct alkylating methyl methanesulfonate (MMS, Sigma-Aldrich) was applied for 24 h as a positive control. The test concentrations were applied for 24 h. The comet assay was performed as described before by our group (15). For each concentration, 50 cells on 2 assay (slide) replicates were evaluated, resulting in 100 cells evaluated per concentration. Cell nucleoli were analyzed semi-automatically with a DMLB fluorescence microscope (Leica Microsystems, Wetzlar, Germany). The image analysis software COMET 5.5 (Kinetic Imaging, Liverpool, UK) was used to measure the comet and identify heads and tails. The percentage of DNA in the tail (TD), the tail length (TL), and the product of the percent of DNA in the tail and the mean migration distance (olive tail moment (OTM)), were evaluated (Kinetic Imaging Limited, KOMET, Singe Cell Gel Elektrophoresis Analysis, Version 4). For statistical analysis, OTM values were evaluated.

 Functionality evaluation in the capillary tube formation assay

To evaluate the effects of α-solanine on vascular endothelial cells' proliferation and tube formation as a model of neoangiogenesis and tissue repair, the capillary tube formation assay was used (16).

10 µl of Matrigel (Sigma-Aldrich, St. Louis, MO, USA) was transferred for µ-slide angiogenesis (µ-Slide Angiogenesis plate, ibidi GmbH, Martinsried, Germany). The plates were placed in an incubator under standard conditions (humidity chamber, 37°C) for 30–60 min. Afterwards, 50 µl of the test cell suspension (containing 1×104 cells each) were transferred into each well. The plate was incubated and analyzed after 6 and 24 h. Images were acquired using an inverted phase contrast microscope (Leica Microsystems, Wetzlar, Germany).

All test concentrations were performed three times. The image sections with the best capillary tube formation were carefully selected and used for automatic image analysis, i.e., three images per test concentration were analyzed. For this purpose, NIH ImageJ software (ImageJ 1.53v) with the Angiogenesis Analyzer plugin (CARPENTIER, 2012) was used as suggested by DeCicco-Skinner et al (16).

 Statistical analysis

GraphPad Prism software 9 (GraphPad Software, Inc., La Jolla, CA, USA) was used for statistical analyses. One-way ANOVA and Dunnett's tests were used to test for statistical differences between the cell viability (MTT assay) of treated samples in comparison with the negative control. The mean values of the OTM of treatment group and control groups and between cells with or without fpg-treatment (comet assay) were also compared with one-way ANOVA and Dunnett's tests. The results of flow cytometry (viable, apoptotic and necrotic cells) in comparison to the negative control and also the results of the capillary tube formation assay, each in comparison to the negative controls, were also analyzed using one-way ANOVA and Dunnett's tests. The P-value for statistical significance was set at P<0.05 and marked with asterisks in plots.

 Results <sec> <title>Cytotoxicity analysis

The MTT assay was conducted to estimate the viability of cells immediately after 24 h of exposure to α-solanine. Fig. 1, Fig. 2, Fig. 3 show the results for the different cell lines expressed as the percentage of viable cells compared to the untreated control groups which were defined as 100%. In the MTT assay, a dose-dependent decrease in cell viability was observed in FaDu, CAL33, and HUVECs, respectively (Fig. 1). Significant cytotoxicity of α-solanine was reached at 21 µM in FaDu, at 21 µM in CAL-33 and at 10 µM in HUVECs.

 Cell cycle analysis

No dose-dependent changes in the phases of the cell cycle were observed after exposure of FaDu to α-solanine doses of 12 to 21 µM compared to the control group (Fig. 2).

 Annexin V-propidium iodide FACS

At an α-solanine concentration of 12 µM and above, FaDu and CAL-33 showed a slight increase in the rate of apoptosis and necrosis. At an α-solanine concentration of 15 µM, the apoptosis rate of cells did not change while the number of necrotic cells increased. At an α-solanine concentration of 30 µM and above, only necrotic cells were detected (Figs. 3 and 4). 1 µM t-BHP as a positive control induced necrosis in all cells. Control groups with untreated cells showed viability of 91.8% for FaDu and 95.9% for CAL-33, respectively. The flow cytometry plots for all groups quantified in the diagrams are shown in Figs. S1 and S2.

 Genotoxicity

HUVECs were exposed to subcytotoxic α-solanine concentrations of 1, 2, 4, and 6 µM for 24 h to evaluate DNA damage.

200 µM mMS for 24 h served as a positive control for genotoxicity. The negative control was the mean OTM of untreated HUVECs. No DNA damage was detected at the doses used, whereas significant damage was induced by mMS (positive control) (Figs. 5 and 6).

 Functional impairment

At subcytotoxic doses, the capillary tube formation assay on HUVECs indicated no significant decrease in the number of meshes and nodes or the total length of the branches (Figs. 7 and 8). The images of all analyzed groups are shown in Fig. S3.

 Discussion

The aim of the current study was to evaluate the tumortoxic effect of α-solanine on two HNSCC cell lines in vitro. In addition, the toxicity of α-solanine as well as possible effects on DNA integrity and cellular function with regard to angiogenesis in non-malignant cells, in this case HUVECs, was investigated (Summarized in Fig. 9).

Previous studies have so far yielded promising results regarding a possible benefit of glycoalkaloids in the therapy of human malignant tumors. This has brought a lot of attention to this group of substances in recent years. In particular, α-solanine has been shown to have a strong antitumor effect and, being a globally available resource due to the wide distribution of solanaceae plant family, has attracted a lot of interest.

HNSCC is the sixth most common cancer in humans and the most common head and neck tumor entity (11). Standard therapies include surgery with or without adjuvant radiotherapy. Alternatively, irradiation or chemoradiation are adequate primary treatment options. Chemotherapy alone is administered only in the palliative setting. The introduction of immune checkpoint inhibitors (ICI) has significantly changed the treatment approach of HNSCC, swiftly becoming the new standard of care as first line therapy in individuals with recurrent and/or metastatic disease. For example, overall survival of patients with metastatic or recurrent HNSCC was prolonged by several months with the PD1 blocker pembrolizumab compared to the control groups with conventional therapies (17). Despite the tremendous progress that the introduction of ICI has resulted in, the median overall survival of many patients remains unsatisfactory and the response rates are still low, even after specific patient selection via PD-L1 expression in the tumor and the immune infiltrate (18). In addition, there is a significant cost to the health care system. Therefore, the discovery of new approaches to drug tumor therapy for HNSCC is essential. Approaches with the least possible side effects on non-malignant cells are of greatest interest.

Glycoalkaloids such as α-solanine inhibit tumor cell growth. However, to date, no study has addressed the effects of α-solanine on HNSCC. The present study is the first investigation of the effects of α-solanine on HNSCC cell lines in vitro.

Toxicity analysis in tumor cell lines FaDu and CAL33 showed a dose-dependent decrease of cell viability after 24 h at α-solanine concentrations of 21 and 21 µM, respectively. In non-malignant HUVECs also treated for 24 h, relevant cytotoxicity was already observed at a concentration of 10 µM α-solanine.

There are several studies on the tumor toxicity of α-solanine in other types of cancer. In prostate carcinoma cells, for example, a significant reduction in cell viability was demonstrated from a concentration of 16 µM α-solanine for 24 and 48 h respectively (19). The toxicity threshold in endometrial carcinoma cells after treatment for 24 h was 30 µM, twice as high (20). In another study, melanoma cells showed a significant and dose-dependent decrease in cell viability starting at a concentration of 23 µM α-solanine.

The selective effect of therapeutics on tumor cells without affecting non-malignant cells is essential. One research group investigated the toxicity of α-solanine on non-malignant human keratinocytes and human fibroblasts. In this study an α-solanine concentration above 23 µM after treatment for 24 h revealed dose-dependent toxicity in both non-malignant cells (21). However, the non-malignant control cells selected here are known to be quite resilient and grow well under a wide range of conditions. Ideally, a potential new anticancer drug should also be tolerable for less resistant cells that are exposed to the drug.

In HUVECs, α-solanine induced toxicity starting at a concentration of 10 µM. This concentration is far below the toxicity threshold of α-solanine on keratinocytes and fibroblasts. Thus, the threshold for α-solanine toxicity could vary in a cell-type-specific manner. This greatly complicates the application of the compound in the clinical setting.

To determine whether cell death is caused by apoptosis or necrosis, the Annexin V assay was performed in the present work. Although administration of low doses of α-solanine initially showed an increase in apoptosis and necrosis in tumor cells, there was a marked dose-dependent increase in tumor cell necrosis without an alteration in the rate of apoptosis. This observation suggests that apoptosis-independent cell death mechanisms may also be important in α-solanine's mode of action at higher concentrations. However, this does not provide any information as to whether the non-apoptotic effect was achieved, for example, by necroptosis, autophagy or ferroptosis.

In previous studies on non-HNSCC cell lines, authors have discussed various cell death mechanisms induced by α-solanine, many of which depend on apoptosis.

In the liver carcinoma cell line HepG2, the formation of reactive oxygen species (ROS), stimulation of apoptosis-inducing proteins ASK1 (apoptosis signal-regulating kinase 1) and TBP-2 (tetrahymena piggyBac transposase 2), and inhibited expression of proliferation-associated proteins, such as HDAC1, has been demonstrated (7). Another study using HepG2 cells also supports the mode of action through apoptosis-dependent cell death, as decreased Bcl-2 expression was demonstrated (22). In the colon carcinoma cell line HT-29, a mechanism for apoptosis induction by the α-solanine-like glycoalkaloid α-chaconine was demonstrated. This was mainly induced by activation of the pro-apoptotic caspase-3 pathway and inhibition of phosphorylation of ERK1 and ERK2 (extracellular signal-regulated protein kinases 1 and 2) (23). The effect of ERK1 and ERK2 extends to different cell functions, such as cell cycle progression, migration, survival, differentiation, metabolism, proliferation and transcription (3). The pro-apoptotic effect via the caspase-3 pathway was also demonstrated for α-solanine in the SW1990 and Panc-1 pancreatic carcinoma cell lines (9). In the prostate cancer cell line DU145, α-solanine showed apoptotic effects mediated by synergistic cyclin suppression, induction of ROS and activation of P38 (24).

Apoptosis-independent modes of action for α-solanine also were reported, for example through increased autophagy rate, inhibition of angiogenesis, and regulation of the cell cycle. In tumor cell lines of various origins, α-solanine induced autophagy through endoplasmic reticulum stress and suppression of the Akt/mTOR pathway, which plays a role in tumor cell proliferation (25). A study by Lv et al (26) suggested an effect of α-solanine by suppressing proliferation, angiogenesis and metastasis. Among other things, they showed that α-solanine significantly reduced the expression of vascular endothelial growth factor (VEGF) and that the tube formation of endothelial cells was altered after treatment with α-solanine. The authors considered this to have potential benefits in the treatment of pancreatic cancer. In melanoma cell lines, α-solanine at subtoxic doses suppressed cell migration and invasion by decreasing the activity of mMP-2 and mMP-9 and causing suppression of phosphorylation of JNK, PI3K, and Akt. In contrast to other studies, the authors found no effects on the phosphorylation of ERK (22).

In the present study, no significant changes in cell cycle phases were observed after 24 h exposure of FaDu to α-solanine doses ranging from 12 to 21 µM. In the literature, a connection between the apoptotic effect of α-solanine and other glycoalkaloids, e.g., with MAP kinase P38 or ERK1/ERK2 acting on the cell cycle, has been observed (24,25). Cell cycle observations of HepG2 cells after treatment with α-solanine showed that cells in the G(2)/M phases disappeared, while cells in the S phase increased (23).

Various effects of α-solanine in humans have been described in literature, ranging from toxicity to teratogenicity. After evaluating the cytotoxicity threshold in non-malignant cells, we found no effect of α-solanine on functionality with regard to angiogenesis and proliferation in HUVECs, nor were there any genotoxic effects. Unfortunately, the toxicity threshold was higher in both malignant cell lines than in benign cells. One way to address this problem is to use a co-drug to prevent damage to the human cells without compromising tumor toxicity. For example, previous studies have shown that increased intake of folic acids is an effective method of preventing NTDs, but not cytotoxicity in general (3). However, this fact cannot be transferred to the in vivo situation. Further possibilities would be to evaluate the optimization of exposure times or recovery times and also the use of lower doses of α-solanine as a co-drug with other potent drugs. Here, further studies are needed.

In summary, the lack of functional effects and genotoxicity on non-malignant cells in the subtoxic regime and the toxic effects of α-solanine on tumor cells are promising. However, the exact mechanisms of action of α-solanine require further investigation. Application as a tumor therapy is currently not probable due to the high toxicity in non-malignant cells. A reduction or prevention of toxicity in non-malignant cells without a simultaneous weakening of the tumor-toxic effects might be a long-term goal. A large number of non-malignant cells are available for safety testing. The usage of primary human cells, which are potentially exposed to the drug during treatment of HNSCC, would be relevant especially.

 Supplementary Material Supporting Data Acknowledgements

The authors would like to thank Mr. Michael Kessler (Research Assistant, Department of Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University Hospital Wuerzburg, Wuerzburg, Germany) for their technical assistance.

 Availability of data and materials

The data generated in the present study may be requested from the corresponding author.

 Authors' contributions

AVF and AS designed the study. AVF performed the experiments. AVF, AS and TG confirm the authenticity of all raw data. AVF and TEK analysed and interpreted the data. AVF wrote the manuscript. SH, CW, TM, TG and RH contributed to data collection and interpretation, and critically reviewed the manuscript. All authors read and approved the final version of the manuscript.

 Ethics approval and consent to participate

The Medical Ethics Committee at the Julius-Maximilians-University (Würzburg, Germany) had no objections regarding the use of anonymous commercial cell lines in this project and confirmed that the requirement for ethics approval can be waived for the study.

 Patient consent for publication

Not applicable.

 Competing interests

The authors declare that they have no competing interests.

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Concentration-dependent cytotoxicity of α-solanine in (A) FaDu cells, (B) CAL-33 cells and (C) HUVECs. Different concentrations were applied as indicated. ANOVA was performed for analysis. *P<0.05 was considered to indicate a statistically significant difference of the respective group compared with the control group (0 µM). Data are presented as the relative percentage compared with the negative control. HUVECs, human umbilical vein endothelial cells; ns, not significant.

Cell cycle analysis after exposure to different concentrations of α-solanine. No changes in the phases of the cell cycle were observed after exposure of FaDu cells to α-solanine (12 and 21 µM).

(A) Concentration-dependent change of viable, apoptotic and necrotic FaDu cells after exposure to α-solanine. Graphical representation of (B) apoptotic and (C) necrotic cells, depending on the α-solanine concentration. *P<0.05 was considered to indicate a statistically significant difference of the respective group compared with the control group (0 µM). Data are presented compared with the negative control. APC, allophycocyanin; t-BHP, tert-butyl hydroperoxide.

(A) Concentration-dependent change of viable, apoptotic and necrotic CAL-33 cells after exposure to α-solanine. Graphical representation of (B) apoptotic and (C) necrotic cells, depending on the α-solanine concentration. *P<0.05 was considered to indicate a statistically significant difference of the respective group compared with the control group (0 µM). Data are presented compared with the negative control. APC, allophycocyanin; t-BHP, tert-butyl hydroperoxide.

Comet assay indicated no DNA damage, represented by the percentage of DNA in the tail (left), tail length (middle) and olive tail moment [=(tail mean-head mean) × (% of DNA in the tail)] (right), in non-malignant human umbilical vein endothelial cells after exposure to subcytotoxic doses of α-solanine. mMS, methyl methanesulfonate.

Examples of DNA comets in the comet assay of (A) the untreated negative control and (B) the positive control treated with 200 µM methyl methanesulfonate (magnification, ×400).

Analysis of the capillary tube formation assay. No marked reduction was observed in ‘nodes’ (purple), ‘meshes’ (blue) and ‘total length of the branches’ (orange) following treatment with (A) 1 and (B) 4 µM subcytotoxic doses of α-solanine in human umbilical vein endothelial cells. Representative images are shown. Scale bar, 500 µm.

No significant changes in meshes (left), nodes (middle) and total length of the branches (right) were detected after exposure to the subcytotoxic doses of α-solanine.

Illustration of the proposed effects of α-solanine on benign (HUVECs) and malignant (FaDu and CAL-33 cells) cells in vitro. HUVECs, human umbilical vein endothelial cells.