Src-associated in mitosis 68 kDa protein (Sam68) is a protein encoded by the heteronuclear ribonucleoprotein particle K homology (KH) single domain-containing, RNA-binding, signal transduction-associated protein 1 (known as KHDRBS1) gene in humans. This protein contains binding sites for critical components in a variety of cellular processes, including the regulation of gene expression, RNA processing and cell signaling. Thus, Sam68 may play a role in a variety of diseases, including cancer. Sam68 has been widely demonstrated to participate in tumor cell proliferation, progression and metastasis to be involved in the regulation of cancer stem cell self-renewal. Based on the body of evidence available, Sam68 emerges as a promising target for this disease. The objectives of the present included summarizing the role of Sam68 in cancer murine models and cancer patients, unraveling the molecular mechanisms underlying its oncogenic potential and discussing the effectiveness of antitumor agents in reducing the malignant effects of Sam68 during tumorigenesis.
Key wordsSam68KHDRBS1cancerRNA-binding proteintumorigenesiscancer-stem cellsUniversity of Seville (Seville, Spain)Plan Andaluz Investigación y Desarrollo, Junta de Andalucia, SpainCTS-151 to VS-MCJ-C is supported by a Margarita Salas fellowship, granted by the University of Seville (Seville, Spain). The group is supported by Plan Andaluz Investigación y Desarrollo, Junta de Andalucia, Spain (grant no. CTS-151 to VS-M).Introduction
The Src-associated in mitosis 68 kDa (Sam68) protein, also known as KH domain-containing, RNA-binding, signal transduction-associated protein 1 (KHDRBS1) due to the gene encoding this protein in humans, was first identified as a molecule phosphorylated by the tyrosine kinase c-SRC during mitosis (1). Sam68 belongs to the signal transduction and activation of RNA metabolism family of RNA-binding proteins (RBPs). This group of proteins contains a glycine-rich protein 33/SAM68/GermLine development defective 1 (also known as GSG) domain for RNA binding and homodimerization (2). Sam68 contains a set of protein domains that allow protein-protein interactions and modifications via multiple pathways. These pathways include six proline-rich sequences enabling interaction with signaling molecules through SRC homology (SH)3 and WW domains, and a tyrosine-rich region at the C-terminus, which form docking sites for signaling proteins that contain SH2 and/or SH3 domains (2).
Due to its high RNA-binding activity and protein-protein interaction domains, Sam68 is considered a multifunctional protein. Sam68 is found in a variety of tissues and cells and has a key role not only in cytosolic cell signaling (3), but also in numerous other processes. These processes include both pre-mRNA and microRNA (miRNA) processing [e.g., spinal muscular atrophy or spermatogenesis, respectively (4,5)]; RNA transport [e.g., human immunodeficiency virus type 1 (6)]; signal transduction [e.g., polycystic ovary syndrome (7)]; alternative splicing [e.g., multiple sclerosis (8)]; and cell cycle progression [e.g., ovarian or prostate cancers (PC) (9,10)]. On another note, Sam68 is also a component of structures such as ribonucleoprotein complexes or subnuclear organelles (11,12). In this sense, Sam68 has a key role in a variety of cellular events, including cell proliferation and growth, be it as a tumor suppressor or as a proto-oncogene regulating cell-cycle progression and apoptosis through RNA-dependent and RNA-independent mechanisms (11-13). Accordingly, Sam68 overexpression has been found in different types of cancer, which may ultimately promote disease progression and metastasis.
Post-transcriptional modifications of Sam68 in cancer
The activity of Sam68 in cellular processes is regulated by post-transcriptional modifications, including tyrosine, serine and threonine phosphorylation mechanisms (Fig. 1), as well as (de)acetylation, arginine methylation and ubiquitination (Fig. 2) (12). During oncogenesis, these mechanisms have been shown to ultimately promote the development and proliferation of malignant cells.
Sam68 phosphorylation
Sam68 seems to play a critical role in cancer, particularly by tyrosine phosphorylation-mediated mechanisms. In 1994, phosphoprotein Sam68 was first identified and described as a mitotic target for tyrosine kinase Src, a mediator of epidermal growth factor (EGF) receptor signaling in breast cancer (BC) cells (14). A variety of studies have demonstrated tyrosine phosphorylation of Sam68 by the Src protein family in numerous settings, including cancer (15-22). The breast tumor kinase (BRK) is an intracellular tyrosine kinase that is overexpressed in BC and boosts EGF response. This kinase was shown to phosphorylate Sam68 on all three tyrosine residues in the nuclear localization signal, with tyrosine 440 being the main Sam68 modulator in BC cells in vitro (23). In the same sense, Sam68 tyrosine phosphorylation was found to be augmented in cholangiocarcinoma (CC) livers and CC cell lines, as compared to normal livers and human cholangiocyte cells. Elevated Sam68 tyrosine phosphorylation was also associated with elevated levels of BRK protein and tumor cell proliferation (24). Of note, a study demonstrated that BRK remains in the cytoplasm of PC cells. However, while Sam68 phosphorylation remained intact, BRK overexpression was not sufficient to transport BRK into the nucleus, its most frequent location in normal prostatic epithelial cells (25). Another study examining prostate tumors reported that Sam68 was only phosphorylated in those tumors that expressed a truncated form of the c-Kit tyrosine kinase, which is found in high-grade tumors (26-28).
Hyperleptinemia and hyperinsulinemia induce a proinflammatory state in obesity that increases BC risk (29). As Sam68 has been found to be tyrosine-phosphorylated in BC (27,28), its phosphorylation has also been investigated in patients with these disorders. It was found that insulin receptor (IR) tyrosine phosphorylates Sam68, which allows it to interact with the SH2 domains of p85, the regulatory subunit of phosphoinositide 3-kinase (PI3K) (30). Leptin has been demonstrated to increase Sam68 tyrosine phosphorylation via Janus kinase/IR substrate (IRS-1)/p85/PI3K signaling; consequently, leptin decreases the ability of Sam68 to bind RNA, while it mediates the survival, growth and proliferation of BC cell lines (31) and JEG-3 choriocarcinoma cells (32). Finally, these changes may ultimately promote abnormal placental development and tumor growth (32,33). Under insulin conditions, IRS-1, which has been found in the nucleus of BC cells (34), binds Sam68 following tyrosine phosphorylation by IR in BC cells in vitro (31). However, it remains elusive whether Sam68 is directly tyrosine-phosphorylated by IR, as suggested by the results obtained in HTC hepatoma cells (35), and whether Sam68 binds intracellular or nuclear IRS-1.
Serine/threonine phosphorylation of Sam68 has also been studied in cancer. Sam68 has been shown to be threonine-followed-by-proline phosphorylated by the RAS-MAPK/ERK kinase (MEK)1/2-ERK1/2 pathway in mouse EL4 T-lymphoma cells to enhance Sam68-dependent exon v5 inclusion in CD44 pre-mRNA (36). In addition, serine/threonine has been demonstrated to be phosphorylated by cyclin-dependent kinase (CDK)1 at T33 and T317. Furthermore, there is evidence that this protein kinase reduces the ability of Sam68 to bind RNA, as it downregulates its alternative splicing activity, thus limiting apoptosis and promoting the proliferation of HCT116 colon cells (37).
Sam68 (de)acetylation
Protein acetylation is a reversible post-transcriptional modification involved in a variety of cellular processes. Its abnormal action has been associated with multiple disorders, including cancer (38). Acetylation is widely known to influence the activity of DNA-binding proteins. Of note, Sam68 was the first RBP reported to be also acetylated (28). Histone deacetylase (HDAC) inhibition and the overexpression of the acetyltransferase CREB binding protein (also known as CBP) promote Sam68 acetylation in renal and, most frequently, in BC cell lines. Hence, CREB-mediated Sam68 acetylation enhances the ability of Sam68 to bind RNA for these cell lines, although other acetyltransferases may be involved in this process (28). CBP also takes part in mixed-lineage leukemia (MLL) gene translocations (39,40) and interacts with the extra eleven-nineteen gene-MLL complex via Sam68 (41,42). Sam68 has been demonstrated to be acetylated in a p300-dependent manner (43). In colorectal cancer (CRC) cell lines, butyrate resistance-associated p300 deficiency has been shown to favor CBP-induced acetylation. Consequently, acetylation added to Sam68 activity may affect gene expression and carcinogenesis in CRC cell lines (44).
Of note, ERK-1/2-mediated phosphorylation of the Scaffold/matrix-associated region-binding protein 1 (SMAR1) releases the inhibitory SMAR1-HDAC6-Sam68 complex, facilitating Sam68 acetylation and alternative splicing in BC cells (45).
Sam68 arginine methylation
Protein methylation is involved in a variety of cellular processes and its aberrant action is implicated in cancer. Therefore, protein arginine methyltransferases (PRMTs) have gained interest in numerous tumors as a therapeutic target (46). Sam68 has been shown to independently recruit PRMT1 and promote RNA splicing (41). By contrast, loss of PRMT1 in mouse embryonic fibroblasts led to the downregulation of Sam68 arginine methylation (47). It has also been demonstrated that PRMT1-mediated methylation may be modulated by the silencing of the human CCR4-associated factor 1 in human MCF7 BC cells, which also regulated Sam68 methylation (48). Of note, the protein PRMT2 has been shown to interact with both Sam68 and PRMT1 to boost its activity. In addition, Sam68 regulates subcellular PRMT2 localization via the SH3 domain in HeLa cells. These findings suggest a role of PRMT2 during inflammation in Sam68-mediated alternative splicing regulation (49).
Sam68 ubiquitination
Aberrant protein ubiquitination also has a crucial role in tumorigenesis (50). Specifically, the ubiquitin-like protein small ubiquitin-related modifier (SUMO) may be a regulator of Sam68 in cancer development. SUMO-1 protein levels have been found to be elevated in acute myeloid leukemia cells and to transform human pluripotent stem cells. This activity may affect nuclear abundance of Sam68 (51). Also, Sam68 was modified by SUMOylation at its first lysine at the amino-terminal region to repress cyclin D1 expression, ultimately inhibiting its ability to induce apoptosis of human renal 293T cells (42,52).
Role of Sam68 in preclinical tumor models and cancer patients
As shown in Tables I and II, the role of Sam68 has been studied in a variety of cancer mouse models and cancer patients, respectively. There is evidence that Sam68 plays a critical role by not only promoting tumor cell development and proliferation, but also by regulating self-renewal in cancer stem cells (CSCs). Consistently, Sam68 regulated the self-renewal of neural stem/progenitor cells via the modulation of the aldehyde dehydrogenase 1 family member A3 (ALDH1A3) (53). ALDH1A3 is a protein that promotes self-renewal and clonogenicity in glioma stem cells (54). This finding implies that the Sam68/ALDH1A3 pathway may be involved in the stemness of cancer cells, although the evidence available is not sufficient.
Sam68 in BC
Sam68 has been demonstrated to be involved in tumorigenesis by modulating CSC self-renewal. NF-κB, a DNA transcription factor acting as a key regulator during oncogenesis (55,56), is involved in the underlying mechanism (57).
Other studies have shown the influence of Sam68 in the self-renewal capacity of breast CSCs; these studies focused on the tumor-derived spheroids responsible for the enrichment of CSCs or cells with stem cell-related characteristics (58,59). Investigation of mammosphere formation in NOD/SCID mice revealed that SKBR-3-Sam68 BC cells had a significantly higher weight compared to SKBR-3-vector tumor cells. In addition, Sam68 activated the Wnt/β-catenin pathway and Sam68 protein expression was negatively correlated with miRNA-204 expression (60). MiRNA-204 is a tumor suppressor miRNA that inhibits cancer cell proliferation and invasion in multiple types of cancer (61-63), which may be useful for diagnosis (64). In the same sense, the Wnt/β-catenin signaling pathway has been found to be activated by Sam68 in MDA-MB-231 BC-bearing mice (a breast CSC-like model), compared to MCF-7 BC-bearing mice (a breast non-CSC-like model) (51). This finding suggests a stronger role of Sam68 during tumor initiation.
Similarly, stem-like cells from spheroids in triple-negative BC-bearing mice expressed high levels of Myc, which required the presence of Sam68 for DNA-damage repair (65). Also, Sam68 gene inhibition caused defects in the poly(ADP-ribose) polymerase (PARP)-induced PAR chain synthesis upon DNA damage. This resulted in cancer cell death, delayed tumor growth and improved survival rates (65), thus demonstrating a key role of Sam68 in CSC-mediated oncogenesis.
Sam68 was also shown to be a regulator of tyrosine kinase activity in vivo and essential in mammary tumorigenesis and metastasis. In a study in nude mice, Sam68 haploinsufficiency delayed tumor progression and metastasis formation driven by the polyoma middle T-antigen (PyMT) oncogene. Furthermore, Sam68 knockdown limited the tumor burden in PyMT-transformed cell lines (13). Similarly, matrix metalloproteinase (MMP)-9, mainly produced by BC cells for invasion and metastasis (66), has been found to be overexpressed in mouse cancer tissue. In addition, MMP-9 correlated with Sam68 expression, which, in turn, upregulated the pro-tumorigenic ephrin tyrosine kinase a3 (EPHA3) gene (67,68) to boost metastasis (69).
Of note, certain proteins with tumor-promoting properties have been found to be associated with Sam68 in patients with BC but not in BC murine models. In this sense, disease-free survival of patients with BC has been associated with BRK6 expression in tumor tissue. In turn, BRK6 expression was significantly correlated with the expression of Sam68 and other signaling proteins such as phosphatase and tensin homolog, ERK and p-ERK (70). MMP-9 has been proven to correlate with Sam68 expression along with lymph node metastasis, suggesting a role in epithelial-mesenchymal transition (69).
In light of the evidence available, Sam68 plays a major role in all stages of oncogenesis. Indeed, Sam68 has been identified as an independent negative prognostic factor for BC (71), particularly in luminal A and triple-negative BC (65), and emerges as a promising therapeutic target in this disease.
Sam68 in PC
The association between Sam68 overexpression and PC cell proliferation and poor outcomes has been consistently demonstrated in different clinical settings (72,73). However, there is a paucity of data from murine models as compared to patients (10,26,74-76). Sam68 has been documented to be phosphorylated by BRK in PC-bearing mice to inhibit the Sam68 RNA-binding capacity and lead to uncontrolled cell cycle progression (74). Of note, Sam68 has been detected in the nuclei of luminal epithelial cells and to be unaltered in high-grade prostate tumors. By contrast, nuclear localization of BRK correlated with higher BRK activity in a more differentiated prostate tumor cell line (LNCaP, poorly differentiated tumorigenic cells), whereas cytoplasmic localization of BRK correlated with decreased BRK activity in a poorly differentiated prostate tumor cell line (PC3, more aggressive cell line) (74).
Regarding LNCaP PC cells, Sam68 has been phosphorylated and detected in grade 7-9 hypertrophic prostate tumors that expressed a truncated form of the c-Kit tyrosine kinase receptor (26). Of note, Sam68 may also interact with the androgen receptor (AR) to co-regulate AR-dependent transcription and modulate AR-dependent alternative splicing. These effects are exerted by enhancing the recruitment of a Sam68-responsive exon transcribed to androgen response elements within the promoter region of the prostate-specific antigen gene (76). This activity ultimately promotes cancer development and progression. In fact, downregulation of Sam68 has resulted in the reduction of both cell cycle progression and PC cell proliferation (75,77).
Similarly, in a PC3 murine model, an antitumor hemisynthetic cardenolide called UNBS1450 showed effectiveness in vitro and in vivo in impairing c-Myc expression and disrupting Sam68 nuclear bodies. These nuclear bodies consisted of Sam68 protein, nucleolar proteins and nucleic acids. According to the authors, Sam68 nuclear bodies delayed the proliferation of PC3 cells but not of normal cells in vitro (78). These findings suggest a critical role of Sam68 in PC, emerging as a promising therapeutic target in the disease.
Sam68 in lung cancer
High Sam68 expression has been shown to be protumorigenic in lung cancer (79) and has been suggested as an independent prognostic marker for overall survival (80). In lung adenocarcinoma (LUAD) cells, Sam68 undergoes O-GlcNAcylation, a post-translational protein modification catalyzed by O-GlcNAc transferase associated with LUAD aggressiveness and poor survival rates (81). In the same sense, Sam68 was demonstrated to switch the metabolism of glucose from oxidative phosphorylation to glycolysis in LUAD cells. Sam68 activity promoted LUAD cell proliferation by regulating the alternative splicing of pyruvate kinase muscle (PKM)2, a key enzyme in glycolysis-dominant energy metabolism in tumor cells (82). This means that patients with elevated Sam68 expression are at a higher risk of tumor recurrence and cancer-related death and shorter overall survival rates, as compared to patients with reduced Sam68 expression (82).
High Sam68 expression has also been found in non-small cell lung cancer (NSCLC) tissue compared to adjacent non-cancerous tissue. In addition, Sam68 expression correlated with lymph node metastasis, advanced tumor grade and poor prognosis (80,83). Similarly, NSCLC cell lines show high Sam68 expression and its knockdown inhibited tumor cell proliferation, colony formation and cell cycle progression via Wnt/β-catenin pathway inhibition (83). By contrast, in lung squamous cell carcinoma, a type of NSCLC, Sam68 expression was similar in normal and tumor tissue; however, Sam68 was spliced by the uc001bub isoform in normal tissue, whereas it was spliced by the uc001bua and uc001buc isoforms in tumor tissue, suggesting a potential role for Sam68 as a prognostic marker in this disease (84).
Of note, an electrochemical immunosensor has been developed for Sam68 protein quantification. The sensor was successfully tested in lung cancer patients, showing a good analytical performance and sensitivity, as compared to ELISA kits (79). This means that Sam68 protein can be tested and quickly quantified in other types of tumors.
Sam68 in CRC
As in BC, Wnt/β-catenin signaling has been demonstrated to be involved in CSC-mediated tumor initiation via Sam68 in patients with colon cancer (85). This signaling pathway was found to be strongly activated by Sam68 in the HT29 colon CSC-like model, as compared to the SW480 non-CSC-like model (51). In line with these results, Sam68 upregulation was correlated with both increased PAR production and NF-κB-mediated anti-apoptotic transcription. Furthermore, Sam68 genetic deletion limited the tumor burden. These results suggest a novel role for this RBP in genotoxic stress-initiated nuclear signaling, which is crucial for colon tumorigenesis (86). Consistently with those notions and similarly to LUAD (see above), Sam68 drove glycolysis in CRC cells via alternative splicing of PKM2, which resulted in CRC cell proliferation (87).
However, the protumoral role of Sam68 in CRC and colon tumors may not be as evident as in other types of cancer. In a study, the lysine-specific methyltransferase Set7/9 methylated Sam68 and its knockout reduced the levels of Sam68 protein in human colon cancer cells. This activity resulted in an altered regulation of the cell cycle and apoptosis, which explains the association between high levels of Sam68-Set7/9 co-expression and improved survival rates (88). On another note, it should be taken into account that the p53 status may influence the role of Sam68 in tumor development (89). At least in colon cancer in vivo, Sam68 may not only have a tumor-promoting role in p53-deficient mice, but also a tumor-suppressive role in mice expressing wild-type p53 (90).
Sam68 in other types of tumor
To a lesser extent, Sam68 has also been studied in other types of tumors in both mice and humans, and its expression has been unfailingly associated with cell proliferation, transformation, tumorigenesis and metastasis.
There is evidence of a high expression of the Sam68 isoform with a shortened 3'untranslated region (3'UTRs) due to alternative polyadenylation in gastric cancer tissue, as compared to paracancer tissue. Overexpression of Sam68 3'UTRs drives tumor progression, as it helps Sam68 miRNA escape from miRNA-mediated gene inhibition (91). Therefore, Sam68 3'UTR expression is elevated in gastric cancer, being associated with a higher grade of malignancy and, ultimately, with poor prognosis (92). Of note, in vitro Sam68 knockdown reduced cell cycle progression and gastric cancer cell migration and invasion (92). The circular RNA Epstein-Barr virus (ebv)-circular RNA ribosomal protein S13 (circRPMS1) was overexpressed in EBV-associated gastric carcinoma (EBVaGC). In addition, the binding of ebv-circRPMS1 to Sam68 activated m6A methyltransferase (METTL3) transcription, resulting in EBVaGC cell proliferation, migration and invasion. This mechanism was ultimately associated with distant metastasis and poor prognosis in clinical EBVaGC samples (93).
In oral tongue squamous cell carcinoma (OTSCC), Sam68 overexpression was significantly associated with lymphovascular invasion and pathological cervical lymph node metastasis in cancer patients (94). Sam68 was also shown to play a role in an OTSCC murine model. According to a study, high Sam68 expression significantly inhibited cisplatin-induced apoptosis. This resulted in the induction of anti-apoptotic proteins such as caspase-9, caspase-3 and PARP, whereas Sam68 silencing markedly boosted the sensitivity of cancer cells to apoptosis (95).
Sam68 has also been found to be highly expressed in skin lesions from glioma-associated oncogene family zinc finger 2-transgenic mice with nonmelanoma skin cancer. A study suggested that Sam68 regulates discoidin domain receptor in keratinocytes and promotes the growth and survival of cancer cells via the NF-κB signaling pathway (96). In addition, Sam68 deficiency in mouse testis has been associated with infertility and defects in spermatogenesis due to the downregulation of genes involved in the cell cycle, cell death, cell-to-cell signaling and interaction, and cancer. This evidence suggests that Sam68 not only plays a role in the development of functional male gametes, but it may also be involved in testis cancer (97). However, the role of Sam68 in skin and testis cancer has not yet been assessed in clinical patients.
Sam68 expression has also been analyzed in certain types of human tumors rather than murine tumors. In these studies, Sam68 was shown to act as a regulator of human CSC vulnerability and increase tumor cell proliferation, migration and invasion. Thus, Sam68 has been identified as a promising prognostic factor for leukemia (51,98); neuroblastoma (99,100); epithelial ovarian cancer (9,101); PC (102); cervical cancer lymph node metastasis (103); muscle invasive bladder cancer (104); endometrial carcinoma (105); esophageal squamous cell carcinoma (106); non-Hodgkin's lymphoma (107); sacral chordoma (108); and hepatocellular carcinoma (109).
Sam68 also plays a key role in the development of renal cell carcinoma (RCC). Evidence suggests that Sam68 is overexpressed in RCC cell lines and cancer tissue, is positively associated with disease stage and severity, and is linked to shorter overall survival rates (110). Sunitinib downregulated phosphorylated Sam68 expression and, in turn, inhibited RCC cell apoptosis in murine models (111). Similarly, Sam68 has been found to be upregulated in human glioblastoma tumor tissue, and its expression was associated with poor prognosis; therefore, it has been suggested as a therapeutic target in this disease (112). By contrast, Sam68 deficiency has been associated with neoplastic transformation of murine NIH3T3 fibroblasts, defective contact inhibition and the development of metastatic tumors in glioblastoma-bearing nude mice after tyrosine phosphorylation (113).
Sam68 as a therapeutic target in cancer
As outlined in section 2, Sam68 is regulated post-translationally via multiple pathways, including tyrosine phosphorylation by the Src tyrosine kinase (114), or SUMOylation by the SUMO-1 protein (51). This regulation provides Sam68 with oncogenic properties that regulate cell cycle and promote tumor cell survival, growth, proliferation and metastasis. Thus, Sam68 has been suggested as a promising therapeutic target in different types of tumors.
From a traditional perspective, chemotherapy (CT) is very effective in increasing the immunogenicity of malignant cells and inhibiting immunosuppressive pathways in cancer (115). However, tumor cell eradication by CT was not as effective as initially expected. Later, combinatorial regimens were developed and successfully tested, with improved clinical safety and efficacy (115,116). On another note, certain tumors do not respond properly to CT due to the development of drug resistance. Resistance may be induced either by pharmacological and physiological factors, such as drug metabolism or inadequate drug access to tumor sites, or by cell- and tissue-specific factors, including gene overexpression and phosphorylation (117,118). In this sense, Sam68 phosphorylation was shown to induce CT resistance in vitro and in vivo in a variety of tumors, including OTSCC and prostate, breast and colon cancer. Thus, silencing of Sam68 phosphorylation enhanced the sensitivity of tumor cells to apoptosis induced by chemotherapeutic agents such as cisplatin, etoposide or camptothecin (65,75,86,95). This evidence suggests that Sam68 knockdown may improve the cytotoxic effects of CT.
Similar results have been obtained in BC using the small molecule dinaciclib (65), a CDK inhibitor (119). Of note, small molecules have been increasingly used as therapeutic targets for two decades due their advantages over CT (120,121). The small molecule B02 has been proven to inhibit RAD51 recombinase, a critical effector of homologous recombination that is upregulated in cancer (122) and in BC xenograft models (123). In these settings, the combination of B02 and downregulation of Sam68, rather than silencing, boosted the reduction of tumor size (65). Intriguingly, other small molecules directly promoted the downregulation of Sam68. Doxycycline, which inhibits CSC phenotypes and epithelial-to-mesenchymal transition (124), reduced Sam68 expression in vitro at the protein and mRNA level in Jurkat and CCRF-CEM T-cell acute lymphoblastic leukemia cells (98). In line with this, Sam68-mediated drug resistance was inhibited in OCI-Ly8 and Jeko-1 non-Hodgkin lymphoma cell lines after treatment with MK2206 (107), an Akt inhibitor (125). Of note, the sodium pump inhibitor UNBS1450 induced the disruption of the Sam68 body in human PC3 prostate cancer cells in vitro, which significantly reduced tumor cell proliferation (78). Based on evidence on the role of Sam68 in the regulation of CSCs via Wnt/β-catenin (51,85), different Wnt/β-catenin inhibitors have been tested. Inhibitors included YB-0158, which disrupted Sam68-Src interactions in CRC cells and significantly inhibited tumorigenesis (85). Other inhibitors included ICG-001 or its analog CWP, which induced the formation of a Sam68/CBP complex to inhibit Wnt/β-catenin in leukemia, colon cancer and BC (51).
Conclusions and future perspectives
In most types of tumors, there is strong evidence that elevated levels of the protein Sam68, along with Sam68 gene overexpression, play a major role in oncogenesis. Thus, Sam68 has been proven to promote tumor cell development, proliferation, progression and metastasis in vitro and in vivo. These findings identify Sam68 as a key regulator in cancer that should be considered as a clinical biomarker and a promising therapeutic target. This conclusion is also supported by the fact that this RBP is directly involved in the self-renewal and survival capacity of CSCs, which are essential for the activation of tumor growth and promote treatment failure and tumor relapse (126).
Of note, post-transcriptional modifications, added to Sam68's characteristics, lead to an increased number of interactions with multiple genes involved in cancer. Some of these genes include (but are not limited to) NIMA related kinase 2 or EPHA3 in BC (69,127); Rad51 in acute myeloid leukemia (123); lysine-specific methyltransferase Set7/9 in colon cancer (88); or METTL3 in gastric carcinoma (88). Thus, the evidence available suggests that Sam68 may also need the involvement of other actors with specific roles in these diseases.
The regulation of cancer driven by Sam68 may depend, at least in part, on i) the type of tissue in which the tumor develops; ii) the tumor niche; and iii) the interactions between Sam68 with different RNAs or other proteins via different signaling pathways, as previously suggested (114). For these reasons, further research is needed to elucidate the potential role of Sam68 and its interactions in these settings in vivo and in vitro.
Based on previous experience of our group with Sam68, it is strongly suggested that high levels/expression of Sam68 have an important role not only in cancer (31-33,128), but also in other diseases (7,129,130). There is cumulative evidence supporting this idea, which has been particularly evaluated in BC; CRC (85-88); gastric cancer (91-93); lung cancer (79-84); and PC (10,26,72-76) tumors, among many others (Table II). Different mechanisms of the role of Sam68 on tumorigenesis in these tumors have been found, such as regulation of alternative splicing of glycolytic enzymes (82,87) and proto-oncogenes (72,73,76), the Wnt/β-catenin pathway (83), genotoxic stress-induced NF-κB activation (86), DNA methylation (93) and modification of miRNAs (91). However, no meta-analyses or clinical trials have been developed to elucidate the exact mechanism of Sam68 mediated tumorigenesis. Meta-analyses may be useful to synthesize the existing data for diseases in which Sam68 is involved, including cancer. On the other hand, there is only one clinical trial, carried out by Awe et al (131), who reported in 2020 that Sam68 is a key determinant of vascular endothelial growth factor receptor 1 isoform expression, extensively found in cancer (132). In light of the cumulative evidence available on the potential role of Sam68 as a therapeutic target in cancer, further studies are needed to provide conclusive evidence.
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Authors' contributions
CJ-C, FS-J, LC-M and VS-M contributed to the conceptualization, literature search and reviewing of the draft. CJ-C wrote the draft. All authors have read and agreed to the final version of the manuscript. Data authentication is not applicable.
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The authors declare that they have no competing interests.
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Ther919561967201010.1158/1535-7163.MCT-09-101220571069MarzagalliMFontanaFRaimondiMLimontaPCancer stem Cells-key players in tumor relapseCancers (Basel)13376202110.3390/cancers13030376334985027864187NaroCBarbagalloFCaggianoCDe MussoMPanzeriVDi AgostinoSParonettoMPSetteCFunctional interaction between the oncogenic kinase NEK2 and Sam68 promotes a splicing program involved in migration and invasion in Triple-negative breast cancerFront Oncol12880654202210.3389/fonc.2022.880654355303159068942Quintana-PortilloRCanfrán-DuqueAIssadTSánchez-MargaletVGonzález-YanesCSam68 interacts with IRS1Biochem Pharmacol837887201210.1016/j.bcp.2011.09.030Vilariño-GarcíaTGuadixPDorado-SilvaMSánchez-MartínPPérez-PérezASánchez-MargaletVDecreased expression of Sam68 is associated with insulin resistance in granulosa cells from PCOS patientsCells112821202210.3390/cells11182821361393969496917NajibSRodríguez-BañoJRíosMJMuniainMAGobernaRSánchez-MargaletVSam68 is tyrosine phosphorylated and recruited to signalling in 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Sam68 phosphorylation in cancer. (A) Tyrosine phosphorylation mechanisms can be driven by i) the Src family kinases and BRK, which mediate epidermal growth factor receptor signaling, and ii) the IRS-1/PI3K pathway, which is promoted under hyperleptinemia and hyperinsulinemia. (B) Serine/threonine phosphorylation mechanisms can be driven by i) T-cell receptor via RAS/BRAF/MEK/ERK signaling to induce the inclusion of the CD44 v5 exon, and ii) CDK1 migrating into the nucleus, which finally promote the production of the cyclin D1 isoform. These mechanisms reduce the ability of Sam68 to bind RNA and promote the transcription of multiple genes involved in oncogenesis. BC, breast cancer; CDK1, cyclin-dependent kinase 1; JAK, Janus kinase; IRS-1, insulin receptor-1; PC, prostate cancer; PI3K, phosphoinositide 3-kinase; Sam68, Src-associated in mitosis 68 kDa; BRK, breast tumor kinase; ERK, extracellular signal-regulated kinase; MEK, mitogen-activated protein kinase kinase; BRAF, B-Raf proto-oncogene.
Sam68 acetylation, arginine methylation and ubiquitination in cancer. (A Acetylation is mediated by i) CBP overexpression, which may be produced via p300 deficiency, ii) interactions between CBP and the EEN-MLL complex, or iii) ERK-1/2-mediated phosphorylation of SMAR1 to release this protein into the cytoplasm. On the contrary, the SMAR1-HDAC6 complex binds Sam68 to promote its deacetylation. (B) PRMTs interact with Sam68 to induce its arginine methylation. (C) Ubiquitination is mainly produced by the SUMO protein (SUMOylation) via interaction with PIAS-1 and HDAC to repress cyclin D1 expression. These mechanisms finally promote the transcription of multiple genes involved in oncogenesis. CBP, CREB binding protein; ERK, extracellular signal-regulated kinase; HDAC, histone deacetylase; MLL-EEN, mixed lineage leukemia-extra eleven nineteen complex; PIAS, protein inhibitor of activated STAT1; PRMT, protein arginine methyltransferase; SMAR1, scaffold/matrix-associated region-binding protein 1; SUMO, small ubiquitin-related modifier; Sam68, Src-associated in mitosis 68 kDa.
Impact of Sam68 expression and protein levels in murine tumor models.
Type of cancer
Mouse model
Impact of Sam68 expression/protein level
(Refs.)
Breast
DB7 and Met-1 cells
• Sam68 may be involved in the epithelial-mesenchymal transition to promote altered cellular morphology, loss of cell adhesion and gain of migratory ability• Sam68 haploinsufficiency delayed mammary tumor onset• Sam68 may be a modulator of tyrosine kinase activity to promote tumorigenesis and metastasis
(13)
MDA-MB-231 and MCF7 cells
Sam68 was overexpressed in mouse models, particularly in those bearing the CSC-like population (MDA-MB-231) compared with the non-CSC-like population (MCF7)
(51)
MDA-MB-231 cells
Sam68 deficiency inhibited tumor growth and metastasis
(69)
BRCAmut and BCSphCs
• Sam68 silencing delayed tumor growth in xenograft mice• Sam68 knockout mice experienced delayed tumor growth and improved survival
(65)
SKBR-3 cells
Mammosphere formation assays in NOD/SCID mice demonstrated significant tumor weights from inoculated SKBR-3-Sam68 cells compared to tumors from injected SKBR-3-vector cells
(60)
Colorectal
Apcmin716/+
• Sam68 expression was higher in tumors compared to adjacent normal tissue• Sam68 expression promoted tumor growth and reduced survival rates
(86)
AOM
p53 status may influence the role of Sam68 during tumorigenesis, since Sam68 deficiency enhanced AOM tumors in wild-type mice, whereas Sam68-haploinsufficiency delayed the onset of spontaneous tumors in p53-deficient mice
(90)
HT29 and SW480 cells
Sam68 expression was markedly higher in the CSC-like mouse model (HT29 tumor-bearing mice) compared with the non-CSC-like mouse model (SW480 colon cancer cells)
(51)
Gastric
EBVaGC
Sam68 expression levels were positively correlated with the expression of cancer-related proteins, such as METTL3 and Ki-67
(93)
AGS cells
Sam68 overexpression promoted cell growth and lung metastasis in the mouse model, particularly its transcript with a shorter 3'UTR
(91)
Glioblastoma
T98G cells
• Sam68 deficiency was associated with tumorigenesis• Decrease of Sam68 protein levels was a feature of cell mitosis
(113)
Lung
NCI-H1975 cells
The Sam68 knockout NCI-H1975 model had a lower risk of cancer-related death compared to control mice
(82)
Prostate
PC3 y LNCaP cells
Sam68 was found in high-grade prostate tumors and its localization was unchanged compared with its expression pattern in normal tissues, whereas breast tumor kinase showed loss of nuclear localization in the less-differentiated tumors
(74)
PC3 cells
Disruption of Sam68 revealed disorganization of cancer cell-specific perinucleolar bodies that suggested nonapoptotic cancer cell death together with the downregulation of c-Myc expression
(78)
Renal
NC-65 cells
Downregulation of Sam68 reduced sunitinib sensitivity, which weakened tumor growth inhibition by inhibiting cell apoptosis
(111)
Skin
Gli2 tg/+
• Sam68 was elevated in skin lesions of Gli-2 transgenic mice• Sam68 participated in regulating DNA damage responses to promote growth and survival of non-melanoma skin cancer
(96)
Testicular
Sam68−/−
Sam68-deficiency mouse testis were found to havedownregulation of numerous genes, including those involved in cancer
(97)
Tongue
SCC-9 cells
• Upregulation of Sam68 inhibited cisplatin-induced apoptosis in mice bearing oral tongue squamous cell carcinoma cells, associated with the induction of anti-apoptotic proteins caspase-9, caspase-3 and PARP• Sam68 silencing enhanced the sensitivity of cancer cells to apoptosis induced by cisplatin
(95)
CSC, cancer stem cell; AOM, azoxymethane; Sam68, Src-associated in mitosis 68 kDa; METTL3, m6A methyltransferase; Gli-2, glioma-associated oncogene family zinc finger 2; PARP, poly(ADP-ribose) polymerase.
Impact of Sam68 expression and protein levels in oncological patients.
Type of cancer
Impact of Sam68 expression/protein level
(Refs.)
Bladder
• Sam68 expression was elevated in muscle invasive bladder cancer compared to normal urothelium and non-invasive tumors• Sam68 may be a promising prognostic marker for muscle invasive bladder cancer
(104)
Breast
• Sam68 was overexpressed in 52% of tumors• Not only Sam68 but also PTEN, MAPK and p-MAPK were significantly correlated with PTK6 expression and poor outcomes
(70)
• Sam68 expression was overexpressed in cancer tissue and associated with lymph node metastasis• MMP-9 was also found overexpressed and associated with Sam68 expression
(69)
• Different levels Sam68 expression were found in cancer cells but barely found in normal/adjacent healthy tissue• There was a negative correlation between Sam68 expression and distant relapse-free survival probability in luminal A and triple-negative breast cancer
(65)
• Association between Sam68 and β-catenin nuclear expression in cancer tissue
(60)
• miR-204 was significantly reduced in cancer tissue and may boost Sam68 3'-UTR activity Sam68 expression was positively correlated with clinical stage, tumor-nodes-metastasis, histological grade and estrogen receptor expression
(71)
Cervical
Sam68 may induce early-stage cervical cancer lymph node metastasis via epithelial-mesenchymal transition
(103)
Colorectal
• Sam68 levels were significantly increased in 94.1% of patients compared to adjacent healthy tissue from the same patient
(86)
• Sam68 expression was positively correlated with increased PAR and phospho-p65 levels Sam68 affected cell growth and glycolysis pathway by regulating the alternative splicing and expression of PKM2 in colorectal cancer
(87)
Sam68/Set7/9 co-expression was positively associated with better survival rates
(88)
Sam68 expression was higher in cancer stem cell-enriched spheroids derived from patients with colorectal cancer
(85)
Endometrial
Sam68 expression positively correlated with the FIGO stage and deep myometrial invasion compared to superficial myometrial invasion
(105)
Esophageal squamous cell carcinoma
Sam68 may activate the Akt/GSK-3β pathway to promote tumor cell proliferation and progression
(106)
Gastric
Sam68 expression was associated with poor prognosis
(92)
Sam68 expression was significantly correlated with the Lauren classification, lymph node metastasis and distant metastasis in EBV-associated gastric cancer
(93)
Sam68 expression was markedly increased in cancer tissue compared to paracancer tissue
(91)
Glioblastoma
• Sam68 expression was upregulated in tumor tissue• High Sam68 expression was correlated with an unfavorable prognosis
(112)
Hepatocellular
Sam68 expression was significantly increased in cancer patients and positively correlated with the Edmondson grade, tumor size, tumor nodule number, HBsAg status and Ki-67 expression, and associated with poor prognosis
(109)
Kidney
• Sam68 expression was similar in normal and cancer tissue from patients with kidney renal papillary cell carcinoma• However, Sam68 was significantly spliced by the uc001bub isoform compared with the uc001bua and uc001buc isoforms in cancer tissue, suggesting that Sam68 may be a prognostic marker
(84)
Leukemia
Sam68 expression was upregulated in patients with T-cell acute lymphoblastic leukemia
(98)
• Sam68 was highly overexpressed in blood cancer stem cells• Sam68 may alter transcription in the interaction between cancer stem cells and CBP
(51)
Lung
High Sam68 expression was found to predict poor prognosis of non-small cell lung cancer
(80)
• High Sam68 expression resulted in poor prognosis in non-small cell lung cancer• Sam68 promoted cell proliferation via activating the Wnt/p-catenin signaling pathway
(83)
Patients with higher Sam68 expression exhibited higher death rates and shorter survival times compared with their low-Sam68-expression counterparts
(82)
Patients with high OGT and high Sam68 expression had poorer overall survival compared to those with low OGT and low Sam68 expression
(81)
• Sam68 was associated with poor survival rates• An electrochemical immunosensor for the detection of Sam68 was developed for the first time and exhibited an excellent analytical performance to predict the pathological state
(79)
• Sam68 expression was similar in normal and cancer tissue from patients with lung squamous cell carcinoma• However, Sam68 was significantly spliced by the uc001bub isoform compared with the uc001bua and uc001buc isoforms in cancer tissue, suggesting that Sam68 may be a prognostic marker
(84)
Neuroblastoma
• Sam68 expression was significantly higher in cancer tissues compared to their matched adjacent healthy tissues• Sam68 knockdown reduced the proliferation, migration and invasion of human neuroblastoma cells in vitro
(99)
Sam68 expression was positively correlated with age, clinical stage, tumor histology and distant metastasis, and associated with shorter survival time
(100)
Non-Hodgkin lymphoma
• Sam68 expression was found to be increased in diffuse large B-cell lymphomas and four reactive lymphoid hyperplasia tissues• Sam68 expression was correlated with poor clinical outcomes and positively associated with tumor cell proliferation
(107)
Oral squamous cell carcinoma
• Nuclear Sam68 expression was significantly observed in cancer cells, whereas its expression in adjacent healthy mucosal epithelium was mainly moderate• Cytoplasmic Sam68 expression was mostly negative• High Sam68 expression was notably correlated with advanced pathological T stage, positive lymphovascular invasion and pathological cervical lymph node metastasis
(94)
Ovarian
• Sam68 was overexpressed at the mRNA and protein levels in epithelial ovarian tumor tissue• Sam68 may accelerate cell cycle progression, since its expression was associated with the FIGO stage, residual tumor size, histological grade and lymph node metastasis
(101)
High Sam68 expression was correlated with poor prognosis in patients with epithelial ovarian cancer
(9)
Pancreatic
Sam68 was required by Fyn and hnRNPA2B1 to regulate apoptosis, induce the proliferation of pancreatic cancer cells and promote metastasis
(102)
Prostate
Sam68 was found in high-grade prostate tumors and its localization was unchanged compared with its expression pattern in normal tissues
(74)
Sam68 was detected in all grade 7-9 hypertrophic prostate tumors that expressed tr-Kit
(26)
Sam68 expression promoted cancer cell proliferation and resistance to cytotoxic agents
(75)
Sam68 was significantly overexpressed in prostate cancer biopsies
(76)
Sam68 expression was significantly associated with XRN2 and a high Gleason score, which indicates disease progression in prostate cancer
(10)
Positive correlation between Sam68 expression and the Cyclin D1 proto-oncogene in cancer tissue
(72)
Correlations between Sam68 expression and the transcription factor c-MYC in cancer patients
(73)
Renal
• Sam68 was notably overexpressed in cancer tissues at both the transcriptional and translational levels• Patients with high Sam68 expression had shorter overall survival rates compared to those with low SAM68 expression• Cytoplasmic Sam68 was significantly correlated with the clinicopathologic grade and outcomes
(110)
• Sam68 expression was significantly higher in sunitinib-sensitive tumors than in sunitinib-resistant tumors• Sam68 was mostly located in the nucleus in sunitinib-sensitive tumors• Sam68 had a positive correlation with time to progression in clear-cell renal cell carcinoma
(111)
Sacral chordoma
• Sam68 was significantly upregulated in cancer tissues compared with normal tissues• Sam68 was significantly associated with surrounding muscle invasion and shorter local recurrence-free survival time
(108)
Thyroid
Variable Sam68 and Sam68DKH isoform expression in not only thyroid cancer tissue (depending on the subtype: Follicular or papillary), but also normal thyroid tissue
(21)
FIGO, International Federation of Gynecology and Obstetrics; Sam68, Src-associated in mitosis 68 kDa; XRN2, 5'-3'exoribonuclease 2; hnRNPA2B1, heterogenous nuclear ribonucleoprotein A2/B1; CBG, corticosteroid-binding globulin; OGT, O-linked N-acetylglucosamine (GlcNAc) transferase; HBsAg, Hepatitis B surface antigen; EBV, Epstein-Barr virus; GSK-3β, glycogen synthase kinase 3β; PKM2, pyruvate kinase muscle 2; PAR, polymers of ADP-ribose; PTK6, protein tyrosine kinase 6; p-MAPK, phosphorylated MAPK; PTEN, phosphatase and tensin homolog.