Shikonin (SHK, >98% purity) was obtained from Yuanye Biotechnology (https://www.shyuanye.com/). Cyclodextrin (α-CD), cetyltrimethyl ammonium bromide (CTAB) and sodium borohydride (NaBH₄) were supplied by Shanghai Aladdin Biochemical Technology Co., Ltd. 4-(dimethylamino) pyridine (DMAP), N,N-dicyclohexyl carbodiimide (DCC), lipoic acid, tetrachloroauric acid (HAuCl4·4H2O), ascorbic acid and silver nitrate (AgNO3) were obtained from MilliporeSigma. Diethyl ether, tetrahydrofuran (THF), dimethyl-sulfoxide (DMSO) and hydrochloric acid were obtained from Nantong Jiangtian Chemical Co., Ltd. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was supplied by Beyotime Institute of Biotechnology. Cell culture reagents were purchased from Gibco; Thermo Fisher Scientific, Inc.).

Two TNBC cell lines (murine TNBC cells 4T1 and human TNBC cells MDA-MB-231) were supplied by the Chinese Academy of Sciences and cultured in RPMI-1640 (4T1 cells) or DMEM (MDA-MB-231 cells) containing 10% (vol/vol) FBS (Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Inc.) at 5% CO₂ and 37°C as previously described (27).

BALB/c mice (female, 6-8 weeks old, weighing 18-20 g) were supplied by Vital River Laboratories. All mice were maintained under specific pathogen-free conditions in an individually ventilated cage facility with a 12/12-h dark/light cycle at 23°C and had ad libitum access to water and food. Animals were monitored at least once daily throughout the study. Predefined humane endpoints were strictly implemented, and every effort was made to minimize pain, distress, and suffering. All animal procedures were conducted in accordance with the Guidelines for Care and Use of Laboratory Animals of Air Force Medical University and were approved by the Animal Ethics Committee of Air Force Medical University (approval no. 20241327; Xi'an China). The study design and animal welfare measures were conducted in accordance with internationally recognized principles for animal research and widely accepted welfare guidelines for oncology studies involving laboratory animals (28-30). A total of 220 mice were used across all in vivo experiments. The bilateral tumor (abscopal) model and the long-term immune memory/rechallenge studies were performed using independent cohorts of animals.

Animals were assessed at least once daily for general health and well-being, including activity, posture, grooming behavior, food and water intake, and signs of pain or distress. Tumor growth was monitored every 3 days by palpation and caliper measurements, while body weight was recorded daily. Tumor volume was calculated using the formula: 0.5 × length × width².

Humane endpoints were defined a priori. Mice were euthanized if any of the following criteria were met: i) Tumor ulceration, necrosis, bleeding or infection; ii) impaired mobility or inability to access food or water; iii) body weight loss of ≥20% from baseline; or iv) excessive tumor burden, defined as any single tumor exceeding 2,000 mm³ in volume.

For near-infrared irradiation (NIR) and surgical procedures, including tumor resection and tissue collection, mice were anesthetized with inhaled isoflurane (3-4% for induction and 1-2% for maintenance in oxygen) and placed on a warming pad. Local tumor temperature during irradiation was continuously monitored using an infrared thermometer or thermal imaging camera.

For pharmacokinetic analysis, 150 μl of blood was collected from the tail vein at each sampling time point. Independent cohorts of mice were used for each time point, and no animal underwent more than two tail-vein blood collections within a 24-h period. For hematological and serum biochemical analyses, whole blood (500-800 μl per mouse) was collected once at the experimental endpoint as a terminal procedure via cardiac puncture under deep isoflurane anesthesia (4-5% for induction, 2-3% for maintenance in oxygen until loss of the pedal withdrawal reflex), immediately followed by euthanasia and tissue collection.

For euthanasia, mice were deeply anesthetized with inhaled isoflurane (4-5% for induction, 2-3% for maintenance in oxygen) until complete loss of the pedal withdrawal reflex, followed by cervical dislocation. Death was confirmed by the absence of respiration, heartbeat, and reflex responses before necropsy and tissue harvesting.

The mPECT diblock copolymers were prepared via ring-opening polymerization of 1,4,8-trioxa [4.6] spiro-9-undecanone (TOSUO) and ε-caprolactone at 130°C using mPEG as an initiator (31). SHK could be encapsulated in the mPECT cores via a hydrophobic interaction, and SHK-mPECT nanoparticles were prepared using the nanoprecipitation approach (32,33). Briefly, mPECT (200 mg) and SHK (2 mg) were dissolved in 2 ml THF, which was added dropwise to 20 ml deionized water under constant stirring. After removing THF through vaporization for 24 h, the SHK-mPECTs were produced by lyophilization (33).

Gold nanorods (AuNRs) were prepared using a facile, seedless synthesis method (34). Briefly, 150 μl cold NaBH₄ (0.01 M), 700 μl ascorbic acid (78.8 mM), 80 μl HCl (37%) and 2.5 ml AgNO₃ (4 mM) were added to a mixture solution (100 ml) of 0.2 M CTAB and 1 mM HAuCl₄ (33). After stirring at 30°C for 6 h, the obtained AuNR-CTAB solution was kept at room temperature overnight. The AuNR-CTAB solution was purified via centrifugation (11,100 × g, 15 min) and rinsed twice before reaction to eliminate residual CTAB coating. Lipoic acid (LA)-modified mPECT (LA-mPECT) was prepared as previously described by the authors (33). Briefly, 0.137 g LA and 0.413 g DCC were dissolved in 1 ml DCM and then stirred for 12 h. Next, 1 g mPECT and 0.12 g DMAP in 10 ml DCM was added. After stirring under nitrogen at 35°C for another 48 h, the mixture was precipitated in cold ether and vacuum-dried at 25°C. AuNR-mPECTs were prepared by dropwise addition of 2 ml LA-mPECT solution (10 mg/ml) in DMSO to a dispersion of 100 ml AuNRs (0.01 mg/ml) under vigorous stirring, followed by an overnight incubation. After recovery of AuNR-mPECTs via centrifugation (16,600 × g, 15 min) and washing twice with deionized water, the mixture was ultimately dispersed in water to the final concentration of 100 μg/ml.

For the preparation of empty mPECT hydrogel, 60 mg α-CD was added to 1 ml of empty mPECT NPs [200 mg/ml, prepared via nanoprecipitation (35)], and then stirred vigorously until the formation of a hydrogel.

For the preparation of SHK@gel (C_SHK=2 mg/ml, C_mPECT=200 mg/ml, C_α-CD=60 mg/ml), 60 mg α-CD was added to 1 ml of SHK-mPECTs solution (200 mg/ml) and then stirred vigorously until the formation of a hydrogel.

For the preparation of AuNR@gel (C_AuNR=50 μg/ml, C_mPECT=200 mg/ml, C_α-CD=60 mg/ml), 200 mg lyophilized powder of empty mPECT NPs was dispersed in 1 ml AuNR-mPECTs solution (50 μg/ml) and then added with 60 mg α-CD under stirring.

To prepare SHK/AuNR@gel (CSH K= 2 mg/ml, C_AuNR=50 μg/ml, C_mPECT=200 mg/ml, C_α-CD=60 mg/ml), 60 mg α-CD was added to an AuNR-mPECTs solution (1 ml, 50 μg/ml) containing 200 mg lyophilized powder of SHK-mPECTs. Finally, the mixture was stirred vigorously until the formation of a nano-composite hydrogel.

TU-1900 spectrophotometer (Purkinje General Instrument) was used to collect UV-Vis spectra. The fluorescence spectral data were recorded using a fluorescence spectrophotometer (Agilent) at 520 nm. The zeta potential and hydrodynamic size of the nanoparticles were determined by dynamic light scattering (DLS; Nano ZS90; Malvern Instruments, Ltd.) at 25°C. The concentration of AuNRs was measured by ICP-MS (7700x; Agilent, Technologies, Inc.). JEOL 100CXII transmission microscope (accelerating voltage=100 kV) was utilized to obtain transmission electron microscopy (TEM) images. The inner morphology of the obtained hydrogel was examined by S-4800 field-emission scanning electron microscopy (SEM; Hitachi, Ltd.). Rheological measurement of the hydrogel was conducted using a fluid rheometer (MCR 302; Anton Paar, Austria). An infrared thermal camera (I5; FLIR, USA) was used to monitor the temperatures of the hydrogel and AuNR solution. To investigate the degradation and SHK content in SHK/AuNR@ gel, 500 μl hydrogel was placed in a 10-ml test tube and then added with 5 ml PBS (pH 7.4). At regular intervals, the supernatant (3 ml) was collected, lyophilized and dissolved in DMF. The SHK levels were examined using the UV-Vis spectrophotometer.

As described previously (11), the 4T1 cells or MDA-MB-231 cells (5×10⁵ cells/well) were cultured in a 6-well plate. On the following day, the cells were transfected with pcDNA3.1-mIDO1 or pcDNA3.1-hIDO1 (Synbio Technologies) using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.). After transfection for 24 h, the cells (2.5×10⁴ cells/well) were cultured in a 96-well plate and treated with the indicated agents for 24 h. Then, the culture medium (200 μl) was mixed with 30% trichloroacetic acid (100 μl; MilliporeSigma). After incubation at 65°C for 15 min and centrifugation (12,000 r/min, 10 min), the supernatant (100 μl) was mixed with 2% (w/v) p-dimethyl-amino-benzaldehyde (100 μl; D2004; MilliporeSigma) in acetic acid. SpectraMax Plus 384 microplate reader (Molecular Devices, LLC) was used to measure the yellow-colored kynurenine at 492 nm.

The cells were seeded in 48-well plates at a density of 3×10⁴ cells/well and cultured under normal conditions for 24 h. Subsequently, the cells were transfected with 0.2 μg pNFκB-luc (Beyotime Institute of Biotechnology) and 0.01 μg pRL-TK Renilla (Beyotime Institute of Biotechnology) using Lipofectamine 2000 for 24 h and then treated with different concentrations of SHK for another 24 h. After treatment, the cells were lysed using passive lysis buffer, and the luciferase activity was detected using a Dual Luciferase Reporter kit (Beyotime Institute of Biotechnology) (36). The experiment was carried out in triplicate and repeated three times independently.

As described previously (37), the total protein or nuclear protein was isolated using RIPA buffer (Thermo Fisher Scientific, Inc.) or nuclear and cytoplasmic protein extraction kit (cat. no. P0027; Beyotime Institute of Biotechnology), respectively. The protein concentrations were evaluated using a BCA protein assay kit. After separation through 10% SDS-PAGE, the protein samples (30 μg per lane) were transferred onto PVDF membranes (Wuhan Servicebio Technology Co., Ltd.). The membranes were blocked in 5% non-fat milk for 60 min, and then exposed to primary antibodies overnight at 4°C. The following antibodies were employed: Anti-PD-L1 (1:1,000; cat. no. GB115704; Wuhan Servicebio Technology Co., Ltd.), anti-NF-κB p65 (1:1,000; cat. no. GB12142; Wuhan Servicebio Technology Co., Ltd.), anti-Histone H3 antibody (1:1,000; cat. no. GB11102; Wuhan Servicebio Technology Co., Ltd.) and anti-β-actin antibody (1:1,000; cat. no. AF7018; Affinity Biosciences). After rinsing, the membranes were exposed to anti-mouse or anti-rabbit HRP-labeled secondary antibodies (1:5,000; cat. nos. SA00001-1 and SA00001-2, respectively; Proteintech Group, Inc.) for 1 h. The protein blots were visualized by ECL reagent (MilliporeSigma). Semi-quantitative densitometry analysis of protein expression was performed using ImageJ v1.46r (National Institutes of Health). Band intensities of target proteins were normalized to the corresponding loading controls and expressed relative to the corresponding control group.

To assess treatment-induced ICD in vitro, 4T1 or MDA-MB-231 cells were seeded in flat-bottom 96-well plates at a density of 1×10⁴ cells per well and allowed to adhere overnight. Cells were then treated for 24 h with various concentrations of free SHK or the indicated nano-formulations, with or without NIR irradiation, at equivalent SHK concentrations. Following treatment, cells were gently washed three times with PBS and fixed in 4% paraformaldehyde (PFA) for 10 min at room temperature without permeabilization to restrict antibody binding to cell-surface calreticulin (CRT). The cells were then blocked with 5% bovine serum albumin (BSA; cat. no. ST023; Beyotime Institute of Biotechnology) in PBS for 1 h and incubated with rabbit anti-CRT primary antibody (cat. no. ab227444; Abcam; 1:200 dilution in 1% BSA/PBS) for 2 h. After three washes with PBS, cells were incubated with HRP-conjugated goat anti-rabbit IgG secondary antibody (Proteintech Group, Inc.; 1:5,000 dilution in 1% BSA/PBS) for 1 h at room temperature. After extensive washing, 3,3',5,5'-tetramethylbenzidine substrate was added, and color development was allowed to proceed in the dark for 10-15 min before termination with 2 M H₂SO₄. Absorbance was measured at 450 nm using a microplate reader. Meanwhile, cell viability in replicate wells subjected to identical treatments was assessed by the MTT assay. CRT signals were normalized to the corresponding MTT absorbance values at 570 nm to account for differences in viable cell numbers and expressed as fold-change relative to untreated controls.

In addition, culture supernatants were collected after treatment and centrifuged to remove cellular debris prior to further analysis. The concentrations of ATP and HMGB1 were determined using a commercial ATP Determination Kit (cat. no. S0027) and HMGB1 ELISA Kit (cat. no. PH406; both from Beyotime Institute of Biotechnology), respectively, according to the manufacturers' instructions.

The viability of cells was assessed by MTT assay as described previously (37,38). Briefly, the cells were cultured in a 96-well plate at 37°C for 24 h. Then, MTT (5 mg/ml) was added (20 μl/well) and incubated for another 4 h. After eliminating the supernatant, the formazan crystals were dissolved in DMSO (150 μl/well). The absorbance was measured using an ELX 800 microplate reader (BioTek; Agilent Technologies, Inc.) at 570 nm.

Caspase-3/7 activity was measured using the Caspase-Glo^® 3/7 Assay kit (cat. no. G8091; Promega Corporation) according to the manufacturer's instructions. Briefly, 4T1 cells were seeded into white 96-well plates and allowed to adhere overnight before treatment with free SHK at the indicated concentrations for 24 h. Following treatment, an equal volume of Caspase-Glo 3/7 reagent was added directly to each well containing cells and culture medium. Plates were gently mixed on an orbital shaker for 30 sec and incubated at room temperature in the dark for 30-60 min to allow cell lysis and substrate cleavage. Luminescence was measured using a microplate reader, and background signal from medium-only wells was subtracted. Caspase-3/7 activity for each treatment condition was normalized to that of untreated control cells and expressed as fold-change relative to control. All treatment conditions were assayed in triplicate wells, and each experiment was independently repeated at least three times.

The left mammary fat pad of each female BALB/c mouse was orthotopically inoculated with 4T1 cells (5×10⁵ cells suspended in 50 μl PBS). Once tumors reached ~150 mm³, the mice were randomly assigned to 5 groups, and administered with 50 μl empty mPECT@gel, free SHK solution, SHK@gel, AuNR@ gel, and SHK/AuNR@gel via intratumoral injection. Mice were anesthetized with inhaled isoflurane prior to irradiation as described above. After injection, mice in the NIR-treated groups were irradiated with an 808-nm laser under real-time temperature monitoring. The laser power was adjusted as needed to rapidly reach and maintain the local tumor temperature at ~43°C for 15 min. This temperature-controlled photothermal stimulation was performed every other day for a total of four sessions, ending on day 6 after intratumoral injection.

Body weight (BW) was recorded daily, and tumor dimensions were measured every 3 days using calipers. Tumor volume was calculated as 0.5 × length × width². On day 8 after intratumoral administration, a portion of tumor tissue was collected under anesthesia for immune-response analysis. Mice were monitored until a single tumor exceeded 2,000 mm³ or predefined humane endpoints were reached, and survival time was recorded.

Detection of Trp and Kyn content in tumor tissues was conducted as previously described (39). Briefly, the tumor tissues were harvested from tumor-bearing mice (TBM) treated with different therapeutics for homogenization using homogenizer. After dissolving the homogenate in trichloroacetic acid (10%), the contents of Trp and Kyn in the supernatant were detected using the LC-MS system (Agilent 1290 Series LC System). Data collection and analysis were conducted with Analyst software (version 1.6.3; SCIEX). The Kyn to Trp ratio (Kyn/Trp) was employed as an indicator of IDO activity.

Immunofluorescence staining was conducted as described previously (40). Briefly, freshly dissected tumor tissue was fixed in 4% PFA at 25°C for 48 h, dehydrated through a graded ethanol series (75, 85, 90, 95 and 100% ethanol; 1 h each), embedded in paraffin wax, and sliced into 4-μm-thick sections. After permeabilization (0.1% Triton X-100; Beyotime Institute of Biotechnology), the sections were blocked for 1 h at room temperature with 10% normal donkey serum (cat. no. ANT051; Wuhan Antgene Biotech Co., Ltd.), and exposed to primary antibodies, anti-PD-L1 (aPD-L1; 1:100; cat. no. ab213480; Abcam) overnight at 4°C. Subsequently, the sections were exposed to fluorescent secondary antibody (1:200; cat. no. sc-362281; Santa Cruz Biotechnology, Inc.) and DAPI (0.001 mg/ml; cat. no. KGA215-10; Nanjing KeyGen Biotech Co., Ltd.), the immunofluorescence image was obtained using a fluorescence microscope (EVOS FL Auto; Thermo Fisher Scientific, Inc.). Data analysis was performed with ImageJ software.

Immunohistochemistry was performed to observe the markers of ICD, CRT and HMGB1, using an indirect peroxidase method (41). The paraffin-embedded tissue section was dewaxed in xylene and rehydrated in a graded series of decreasing concentrations of ethanol. After quenching endogenous peroxidase with hydrogen peroxide (3%), the section was blocked with goat serum (10%; ZSGB-Bio) for 30 min at room temperature to inhibit non-specific antibody binding. The tissue sections were incubated overnight with primary antibody at 4°C. Antibodies against CRT (1:100; cat. no. ab227444) and HMGB1 (1:350; cat. no. ab79823) were supplied by Abcam. Subsequently, the sections were exposed to HRP-conjugated goat anti-rabbit IgG (1:200; cat. no. SA00001-2; Proteintech Group, Inc.). The chromogenic substrate used in the present study was diaminobenzidine (DAB; ZSGB-Bio). After counterstaining with hematoxylin and mounting in resin, images were obtained using an Aperio pathology scanner. The IOD values were determined with ImageJ software.

Primary tumors and ipsilateral inguinal/axillary tumor-draining lymph nodes (TDLNs) were collected 24 h after the final treatment. TDLNs were used for CD11c⁺ dendritic cell (DC) enrichment, qPCR-based analysis of DC maturation markers, and IL-12p70 ELISA. Primary tumor tissues were used for qPCR-based assessment of T-cell infiltration- and cytolytic activity-related genes, as well as CD4/CD25-enriched Foxp3 analysis.

CD11c⁺ cells were isolated from TDLNs using positive magnetic selection with CD11c MicroBeads UltraPure (mouse; cat. no. 130-125-835; Miltenyi Biotec, Inc.) and LS Columns (cat. no. 130-042-401; Miltenyi Biotec, Inc.). CD4⁺ tumor-infiltrating lymphocytes (TILs) were enriched from tumor tissues using the Tumor Dissociation Kit (mouse; cat. no. 130-096-730; Miltenyi Biotec, Inc.), CD4 (TIL) MicroBeads (mouse; cat. no. 130-116-475; Miltenyi Biotec, Inc.), and LS Columns. Where indicated, CD25 surface labeling-based magnetic enrichment was further performed using mouse CD25 MicroBeads (cat. no. 130-091-072; Miltenyi Biotec, Inc.), and RNA was extracted from the CD25-enriched CD4⁺ fraction for Foxp3 analysis. For ex vivo memory T-cell assays, splenic CD8⁺ T cells were purified by negative selection using the MojoSort™ Mouse CD8⁺ T Cell Isolation Kit (cat. no. 480008; BioLegend, Inc.), whereas CD11c⁺ DCs were enriched using CD11c MicroBeads (Miltenyi Biotec, 130-108-338).

Total RNA was extracted from cells, tumor tissues, or enriched immune cell fractions using TRIzol™ reagent (cat. no. 15596026; Thermo Fisher Scientific, Inc.), the RNeasy Micro Kit (cat. no. 74004; Qiagen China Co., Ltd.), or the RNeasy Mini Kit (cat. no. 74104; Qiagen), as appropriate. RNA was reverse-transcribed into cDNA using PrimeScript™ RT Master Mix (cat. no. RR036A; Takara) or PrimeScript™ RT Reagent Kit (cat. no. RR037A; Takara Biotechnology Co., Ltd.). RT-qPCR was performed on a CFX96 Real-Time PCR System using TB Green^® Premix Ex Taq™ II (cat. no. RR820A; Takara) under the following thermocycling conditions: Initial denaturation at 95°C for 30 sec, followed by 40 cycles of denaturation at 95°C for 5 sec and annealing/extension at 60°C for 30 sec. GAPDH was used as the reference gene, and relative expression was calculated using the 2^−ΔΔCq method (42). For DC maturation analysis, H2-Ab1, Cd80, Cd86, Il12a and Il12b were quantified in CD11c⁺ cells isolated from TDLNs, and the DC maturation index was calculated as the mean z-score of the normalized expression values of these five genes (43). The primer sequences for RT-qPCR were as follows: GAPDH forward, 5'-CATCACTGCCACCCAGAAGACTG-3' and reverse, 5'-ATGCCAGTGAGCTTCCCGTTCAG-3'; H2-Ab1 forward, 5'-GTGTGCAGACACAACTACGAGG-3' and reverse, 5'-CTGTCACTGAGCAGACCAGAGT-3'; Cd80 forward, 5'-CCTCAAGTTTCCATGTCCAAGGC-3' and reverse, 5'-GAGGAGAGTTGTAACGGCAAGG-3'; Cd86 forward, 5'-ACGTATTGGAAGGAGATTACAGCT-3' and reverse, 5'-TCTGTCAGCGTTACTATCCCGC-3'; Il12a forward, 5'-CTGTGCCTTGGTAGCATCTATG-3' and reverse, 5'-GCAGAGTCTCGCCATTATGATTC-3'; Il12b forward, 5'-TGGTTTGCCATCGTTTTGCTG-3' and reverse, 5'-ACAGGTGAGGTTCACTGTTTCT-3'; Cd3e forward, 5'-GCTCCAGGATTTCTCGGAAGTC-3' and reverse, 5'-ATGGCTACTGCTGTCAGGTCCA-3'; Cd4 forward, 5'-GTTCAGGACAGCGACTTCTGGA-3' and reverse, 5'-GAAGGAGAACTCCGCTGACTCT-3'; Cd8a forward, 5'-ACTACCAAGCCAGTGCTGCGAA-3' and reverse, 5'-ATCACAGGCGAAGTCCAATCCG-3'; Gzmb forward, 5'-CAGGAGAAGACCCAGCAAGTCA-3' and reverse, 5'-CTCACAGCTCTAGTCCTCTTGG-3'; Prf1 forward, 5'-TTTCGCCTGGTACAAAAACC-3' and reverse, 5'-CGTTCAGGCAGTCTCCTACC-3'; Foxp3 forward, 5'-CCCAGGAAAGACAGCAACCTT-3' and reverse, 5'-TTCTCACAACCAGGCCACTTG-3'; Tnf forward, 5'-CCCTCACACTCAGATCATCTTCT-3' and reverse, 5'-GCTACGACGTGGGCTACAG-3'; Ifng forward, 5'-GCCACGGCACAGTCATTGA-3' and reverse, 5'-TGCTGATGGCCTGATTGTCTT-3'; and Cd274 forward, 5'-TGCGGACTACAAGCGAATCACG-3' and reverse, 5'-CTCAGCTTCTGGATAACCCTCG-3.

IL-12p70 levels in TDLN homogenates were quantified using a Mouse IL-12p70 ELISA Kit (cat. no. 88-7121-22; Invitrogen; Thermo Fisher Scientific, Inc.) and normalized to total protein content (44). For ex vivo memory immune-response analysis, granzyme B and perforin concentrations were quantified using Mouse Granzyme B ELISA (cat. no. MBS2507805; MyBioSource, Inc.) and Mouse Perforin ELISA (cat. no. MBS2021832; MyBioSource, Inc.) kits, respectively. IFN-γ, TNF-α, IL-2, CXCL9 and CXCL10 levels in culture supernatants were measured using the corresponding commercial ELISA kits according to the manufacturers' instructions. IFN-γ-producing T cells were quantified using a Mouse IFN-γ ELISpot Kit (cat. no. 552569; BD Biosciences), and results were expressed as spot-forming units (SFU) per 10⁵ T cells. Polyfunctional T cells simultaneously producing IFN-γ and TNF-α were quantified using a Dual-Color Mouse ELISpot Kit (cat. no. SPOT-09-2; Mabtech), and results were expressed as double-positive SFU per 10⁵ T cells.

For T-cell infiltration analysis, Cd3e, Cd8a and Cd4 were quantified in tumor tissues, and the T-cell infiltration index was calculated as the mean z-score of normalized Cd3e, Cd8a and Cd4 expression values (45). Cytolytic activity (CYT) was quantified by the average of the log-transformed expression values of Gzmb and Prf1, corresponding to the geometric mean of their expression on a linear scale (46). Foxp3 expression was quantified in CD25-enriched CD4⁺ TILs and normalized to the corresponding control group (47).

To measure SHK levels in plasma and tumor tissues, 4T1 TBM were intratumorally injected with free SHK, SHK@gel, or SHK/AuNR@ gel. The treatment schedule was identical to that used in the local tumor therapy study described above. Peripheral blood (150 μl per mouse per time point) was collected from the tail vein at predetermined time points into heparinized tubes. Independent cohorts of mice were used for each sampling time point. Plasma was obtained by centrifugation at 850 × g for 10 min, transferred to clean polypropylene tubes and stored at −80°C until analysis. To determine SHK content in plasma, 50 μl plasma samples were mixed with 200 μl ethyl acetate, followed by vortexing (10 min) and centrifugation (15,000 rpm, 10 min, 4°C). The obtained SHK solution was dried through evaporation and reconstituted in 100 μl 50% methanol/50% acetonitrile containing 1% formic acid (48). To detect SHK content in tumors, the tumor tissues were homogenized in saline (ratio of tumor weight-to-volume=1:1), and the protein was precipitated with 5 volume of 50% methanol/50% acetonitrile containing 1% formic acid. After centrifugation (21,100 × g, 5 min, 4°C), the supernatant was filtered using a 0.22-μm filter, and subsequently analyzed with the HPLC system (Shimadzu LC-20AT).

At the terminal time point, whole blood (500-800 μl per mouse) was collected by cardiac puncture under deep anesthesia for hematological and serum biochemical analyses, followed immediately by euthanasia as aforementioned. Hematology and serum biochemistry were analyzed by the Clinical Laboratory of Xijing Hospital, including hepatorenal function markers [blood urea nitrogen (BUN), creatinine (CREAT), alkaline phosphatase (ALP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST)] and routine blood parameters [monocyte (Mono), lymphocyte (LYMPH), hematocrit (HCT), hemoglobin (HGB), platelet (PLT), red blood cell (RBC) and white blood cell (WBC) counts]. Major organs were collected, fixed in 4% neutral-buffered PFA, and assessed by H&E staining. BW was recorded throughout the experiment.

For the bilateral tumor model, 5×10⁵ 4T1 cells suspended in 50 μl PBS were injected into the right mammary fat pad to establish the primary tumor. Three days later, an equal number of 4T1 cells was injected into the contralateral mammary fat pad to establish the distant tumor, following a previously reported short-interval bilateral orthotopic tumor model (49). When the primary tumor volume reached ~150 mm³, mice were randomly assigned to five treatment groups and received intratumoral injections (50 μl) of empty mPECT@gel, free SHK solution, SHK@gel, AuNR@gel + NIR, or SHK/AuNR@ gel + NIR. Mice were anesthetized with inhaled isoflurane prior to NIR irradiation and surgical procedures, as described above. NIR irradiation was performed using the same temperature-controlled protocol described above. After completion of the treatment schedule, the primary tumor was surgically resected. BW was monitored daily, and the volume of untreated contralateral tumors was measured every 3 days. Immune-related molecular endpoints in distant tumors, including T-cell infiltration-related genes, cytolytic effector genes and Foxp3 expression in CD25-enriched CD4⁺ TILs, were analyzed using the same magnetic enrichment, RT-qPCR and calculation procedures described above.

For depletion of CD4⁺ and CD8⁺ T cells, bilateral tumor-bearing mice were intraperitoneally injected with anti-CD8 antibody (10 mg/kg; BioXcell, clone 53-6.7) or anti-CD4 antibody (10 mg/kg; BioXcell, clone GK1.5), with the corresponding IgG isotype controls (BioXcell, BE0089 or BE0090), once every 3 days starting from the implantation of the distant tumor (50). Intratumoral administration and NIR irradiation were performed as described above. BW was monitored daily, and distant tumor growth was assessed every 3 days. The bilateral tumor model was conducted in accordance with the same animal monitoring and humane endpoint criteria described above (28-30,51).

Female BALB/c mice were orthotopically inoculated with 4T1 cells (5×10⁵ cells per mouse in 100 μl PBS) into the mammary fat pad. When tumors reached ~150 mm³, mice were randomly assigned into five treatment groups and received intratumoral injections (50 μl) of blank mPECT@gel, free SHK solution, SHK@gel, AuNR@gel + NIR, or SHK/AuNR@gel + NIR. For the NIR-treated groups, tumors were irradiated using the same temperature-controlled 808-nm laser protocol described above. Two days after the final treatment, primary tumors were surgically resected under anesthesia to simulate complete local tumor eradication.

On day 30 after treatment, mice were rechallenged subcutaneously with 4T1 cells (5×10⁵ cells per mouse) in the contralateral flank to evaluate systemic antitumor immune memory (52,53). Rechallenge tumor growth was monitored every 3 days by caliper measurements, and tumor volume was calculated as 0.5 × length × width². In a separate cohort, mice received intravenous injection of 1×10⁵ 4T1 cells on day 30 after treatment to establish an experimental lung metastasis model. Fourteen days later, mice were euthanized, and lungs were harvested, rinsed with PBS and fixed in 4% PFA. Surface metastatic nodules were counted under a dissecting microscope (S9D; Leica Microsystems GmbH), and lung tissues were processed for H&E staining to assess pulmonary micrometastasis. Animal monitoring and humane endpoints were applied as aforementioned.

To characterize memory immune responses, spleens were harvested on day 31 after treatment. Single-cell suspensions were prepared by mechanical dissociation, filtration through 70-μm cell strainers and erythrocyte lysis. Purified CD8⁺ T cells and enriched CD11c⁺ DCs were prepared as described in the magnetic enrichment of immune cell subsets section. DCs were pulsed with 4T1 tumor lysates (100 μg/ml protein) for 3 h at 37°C and washed three times to remove free antigen. Purified CD8⁺ T cells were then co-cultured with antigen-loaded DCs at an E:T ratio of 10:1 for antigen-specific recall assays.

The frequency of IFN-γ-producing T cells and IFN-γ/TNF-α double-positive T cells was measured by ELISpot as described in the ELISA and ELISpot assays section. Cytotoxic activity of memory CD8⁺ T cells was evaluated using a colorimetric lactate dehydrogenase (LDH) cytotoxicity assay kit (cat. no. 88953; Thermo Fisher Scientific, Inc.). Effector CD8⁺ T cells were co-incubated with 4T1 target cells at E:T ratios of 5:1, 10:1, 20:1 and 40:1 for 6 h, followed by absorbance measurement at 490 nm. Specific lysis was calculated as [(sample-spontaneous)/(maximum-spontaneous)] ×100%, and overall cytotoxic activity was summarized as the area under the dose-response curve using GraphPad Prism v9.0.

For molecular profiling, total RNA was extracted from purified CD8⁺ T cells and analyzed by RT-qPCR as aforementioned. For degranulation analysis, CD8⁺ T cells were co-cultured with antigen-pulsed DCs for 4 h, after which culture supernatants and cell lysates were collected separately. Degranulation capacity was calculated as [supernatant/(supernatant + lysate)] ×100% based on granzyme B and perforin levels, and the mean release rate of these two markers was used as an overall measure of cytotoxic granule exocytosis. Recall cytokine responses were assessed by co-culturing T cells with antigen-pulsed DCs for 48 h, followed by ELISA-based measurement of IFN-γ, TNF-α, IL-2, CXCL9 and CXCL10 as aforementioned.

All data are presented as the mean ± SD. Statistical tests were performed using SPSS Statistics version 31.0 (IBM Corp.). The differences among the groups were compared by one-way ANOVA and Bonferroni's post-hoc test. The intergroup survival rates were determined using the log-rank test of Kaplan-Meier survival curves. P<0.05 was considered to indicate a statistically significant difference.

Increasing evidence indicates that indoleamine 2,3-dioxygenase 1 (IDO1) plays a vital role in modulating tumor immune evasion, with IDO1-associated depletion of tryptophan and accumulation of toxic metabolites frequently observed in immunosuppressive TMEs (54). SHK, a naphthoquinone compound (Fig. 2A), has been reported to exert a notable inhibitory effect on the catalytic activity of IDO1 (11). Consistent with these observations, the concentration-dependent effects of free SHK were first examined in 4T1 cells. Exposure to SHK for 24 h resulted in a gradual reduction in cell viability, with only minimal effects observed at 0.5-1 μM and moderate cytotoxicity at 2-4 μM, where more than 50% of cells remained viable (Fig. 2B). Within this concentration range, SHK inhibited IDO1 enzymatic activity in a dose-dependent manner, yielding an IC₅₀ value of ~1.94 μM (Fig. 2C), consistent with a previous study (11). Notably, 1 μM SHK produced only low-to-moderate inhibition of IDO1 activity, whereas inhibition approaching 50% was mainly observed at ~2 μM. Programmed death-ligand 1 (PD-L1), another critical mediator of tumor-associated immunosuppression (55), was likewise downregulated by SHK in a concentration-dependent manner (Fig. 2D). Previous studies, including one by the authors, have demonstrated that SHK suppresses nuclear factor-κB (NF-κB) signaling in multiple experimental settings (38,56,57). In agreement with these findings, SHK significantly reduced NF-κB transcriptional activity, as assessed using an NF-κB-responsive luciferase reporter assay, and inhibited nuclear translocation of NF-κB p65 in 4T1 cells (Fig. S1A and B). Given the established role of NF-κB in promoting PD-L1 transcription (58), these results suggest that inhibition of the NF-κB pathway may contribute, at least in part, to the SHK-mediated downregulation of PD-L1. ICD is characterized by the release or exposure of DAMPs that facilitate antigen uptake and promote DC maturation (59). To determine whether SHK induces ICD-related signals in TNBC cells within the aforementioned concentration range, several canonical DAMPs and cell-death-associated parameters were quantified in 4T1 cells. Under non-permeabilizing conditions, SHK induced a concentration-dependent increase in normalized cell-surface CRT exposure, as determined by cell-based ELISA after normalization to viable cell number (Fig. 2E). Consistent with the induction of ICD, extracellular HMGB1 and ATP levels in culture supernatants were significantly elevated following treatment with 2 and 4 μM SHK (Fig. 2F and G). Moreover, caspase-3/7 activity increased in a concentration-dependent manner, with pronounced elevations observed at 1-4 μM (Fig. 2H), indicating engagement of apoptotic signaling pathways within this moderately cytotoxic concentration range. To determine whether these effects extended beyond murine TNBC, parallel studies were performed in human MDA-MB-231 cells. As revealed in Fig. S2A-F, SHK similarly reduced cell viability, suppressed IDO1 activity and PD-L1 expression, and enhanced cell-surface CRT exposure as well as HMGB1 and ATP release in a concentration-dependent manner. Collectively, these findings demonstrate that, at concentrations causing only partial loss of viability, SHK simultaneously inhibits IDO1 catalytic activity and PD-L1 expression, while promoting the release of hallmark ICD-associated DAMPs in both murine and human TNBC cells, consistent with the induction of an immunostimulatory ICD-like cell-death program.

In the present study, AuNRs were prepared using the seedless synthesis method (34), and AuNR-mPECTs were synthesized through the 'ligand substitution method' based on the authors' previous procedures (33). Compared with AuNRs, AuNR-mPECTs exhibited a similar characteristic UV-Vis absorption, as revealed by longitudinal and transverse plasmonic peaks at 785 and 520 nm, respectively (Fig. 3A). The longitudinal plasmonic peak was slightly red-shifted, which might correspond to the surface modification of mPECTs. Dynamic light scattering showed a dominant hydrodynamic size peak at 50 nm (PDI=0.642) for AuNRs and 58 nm (PDI= 0.449) for AuNR-mPECTs (Fig. 3B). The decreased zeta potential and increased particle size of AuNR-mPECTs confirmed the successful substitution of positively charged CTAB on the surface of AuNR-mPECTs (Fig. 3B and C). The micro-morphology of AuNR-mPECTs was examined by TEM. Notably, AuNR-mPECTs showed a typical rod structure, with the long and short diameters of 48±8 and 11±2 nm, respectively (Fig. 3D). The stability of AuNR-mPECTs is critical for their biomedical applications. To validate their dispersion stability under physiological conditions (osmotic pressure, protein and the microenvironment of acid-base abnormalities in tumors), AuNR-mPECTs were dispersed in 10% FBS-containing culture medium, NaCl solution (0.1 M) and PBS solution (pH 7.4 or 6.8), respectively. After 24 h of incubation, the UV-Vis spectral data of all samples were obtained, and similar longitudinal and transverse absorption peaks were observed in each spectrogram (Fig. S3A), indicating its optical stability in different media. Furthermore, the stability of AuNR-mPECTs was evaluated by measuring hydrodynamic diameter after exposure to various media mimicking the in vivo microenvironment. It was found that the hydrodynamic diameter of AuNR-mPECTs showed no obvious changes after incubation with different media (Fig. S3B). Furthermore, the UV-Vis spectral data or particle size of AuNR-mPECTs had no significant changes after laser irradiation, suggsting the excellent photothermal stability of AuNR-mPECTs (Fig. S3C and D). Additionally, no significant changes were observed in UV-Vis spectral data and particle size after 10 days of storage, indicating AuNR-mPECTs have favorable storage stability (Fig. S3E and F). In addition to the aforementioned results, the surface coating with mPECTs could greatly reduce the toxicity of AuNRs, and no significant cytotoxicity of AuNR-mPECTs was observed, even at 50 μg/ml (Fig. S4A and B).

SHK, which is a hydrophobic drug, can be encapsulated in the hydrophobic core of mPECT nanoparticles through hydrophobic interaction to form drug-loaded SHK-mPECT nanoparticles (SHK-mPECTs). TEM images showed that SHK-mPECTs had a uniform spherical structure (Fig. 3E). The hydrodynamic diameter of SHK-mPECTs was ~141 nm with a narrow distribution (PDI=0.262) (Fig. 3F). Fluorescence and UV-Vis absorption characterization revealed that loading SHK into nanoparticles did not affect the optical properties of SHK compared with bare SHK at the same concentration, suggesting that the loading of SHK into nanoparticles did not affect the chemical properties of SHK (Fig. 3G and H). Moreover, the effects of different nano-components on IDO1 activity, PD-L1 expression and ICD induction were further assessed. As demonstrated in Fig. 3I-M, compared with free SHK, SHK-mPECTs showed the same or even slightly stronger effects on inhibiting IDO1 activity (Fig. 3I), downregulating PD-L1 expression (Fig. 3J) and inducing ICD (Fig. 3K-M). Mild PTT stimulation significantly increased IDO1 activity and PD-L1 expression (Fig. 3I and J), but the ability of mild PTT to induce ICD was weak (Fig. 3K-M). However, the combination of mild PTT with SHK-mPECTs significantly reversed the increases in IDO1 activity and PD-L1 expression induced by mild PTT and strongly induced ICD in 4T1 cells (Fig. 3I-M). Similar trends were also detected in human TNBC MDA-MB-231 cells (Fig. S5A-E). To further exclude the potential effects of the blank nanocarrier and NIR irradiation itself, a focused two-group control experiment was performed in 4T1 cells. Empty mPECT-NPs + NIR caused only a slight temperature increase and did not generate controllable mild photothermal heating. In addition, compared with empty mPECT-NPs, empty mPECT-NPs + NIR did not significantly alter IDO1 activity, PD-L1 mRNA expression, or ICD-associated markers, including CRT, HMGB1 and ATP (Fig. S6A-F).

After adding α-CD into AuNR-mPECTs and SHK-mPECTs, the mixture solution was converted into a claret-red homogeneous hydrogel at the macroscopic level (Fig. 4A). A homogeneous network structure was obviously observed in the SEM images of SHK/AuNR@gel (Fig. 4B). The rheological properties of SHK/AuNR@gel were evaluated subsequently. As shown in Fig. 4C, although the angular frequency was increased, the storage modulus G' of SHK/AuNR@gel was always higher than the loss modulus G', suggesting that an elastic solid-like hydrogel might be formed. Shear thinning is an essential property for realizing the injectability of a hydrogel. As revealed in Fig. 4D, with the increase of the shear strain, SHK/AuNR@ gel exhibited a non-Newtonian shear thinning (pseudoplastic) behavior, which indicated that SHK/AuNR@gel was injectable. During the photothermal process, the stability is crucial for avoiding the unnecessary burst release of drugs loaded in the hydrogel at the higher treatment temperatures. As demonstrated in Fig. 4E, with the rising temperatures, the storage modulus G' of SHK/AuNR@gel was always higher than the loss modulus G', suggesting that SHK/AuNR@gel could maintain a stable gelling state at a relatively higher treatment temperature (≤50°C). In addition, the particle size distribution of SHK-mPECTs was similar within the temperature range of 25-60°C, suggesting that SHK-mPECT NPs could remain stable without disassembly or aggregation during hyperthermia treatment (Fig. S7). Subsequently, the release behaviors of SHK from SHK/AuNR@gel were investigated. Notably, the release of SHK in SHK/AuNR@ gel showed a zero-order release curve with no obvious burst release phenomenon (Fig. 4F), indicating that SHK/AuNR@ gel could maintain a controlled, sustainable release behavior. Furthermore, the degradation curve of SHK/AuNR@gel was similar to the release curve of SHK, suggesting that the release of SHK mainly depended on the dissolution and degradation of SHK/AuNR@gel (Fig. 4F and G).

Photothermal conversion efficiency (PCE) is important for the application of hydrogels in PTT. As demonstrated in Fig. 4H, the temperature of AuNRs and SHK/AuNR@gel both rose rapidly to ~60°C within only 6 min under the irradiation of 808 nm NIR, indicating that loading AuNRs into SHK/AuNR@gel did not obviously affect the PCE of AuNRs. The photothermal conversion properties of SHK/AuNR@gel were highly dependent on the irradiated power of the laser (Fig. 4I). In other words, the higher the power irradiated on SHK/AuNR@gel, the faster the temperature rose. To explore the stability of photothermal conversion, SHK/AuNR@gel was irradiated with an 808 nm laser for 10 min at 2.0 W/cm² and then cooled to room temperature; this laser on/off process involved a total of 4 cycles. As demonstrated in Fig. 4J, the peak temperatures did not drop significantly during the whole process, suggesting that SHK/AuNR@gel had favorable stability of photothermal conversion even after the long-term and repeated laser irradiation. Altogether, these results indicate that SHK/AuNR@gel has excellent efficiency and stability for photothermal conversion.

Based on previous studies (25,26), BALB/c mice bearing 4T1 tumor cells were chosen as the immune 'cold' TNBC model for in vivo study. To further verify the previous in vitro findings, the effects of hydrogel with different compositions on IDO1 activity, PD-L1 expression and ICD were first detected in local tumors. Because SHK@ gel does not contain AuNRs, NIR irradiation of SHK@gel alone was not expected to generate controllable mild photothermal heating under the present experimental conditions. Therefore, SHK@gel mainly represented the effect of local sustained SHK release, whereas SHK/AuNR@gel + NIR represented the combination of hydrogel-mediated sustained SHK release and AuNR-mediated mild PTT. The detailed experimental process is shown in Fig. 5A. The activity of IDO1 could be indirectly reflected by the ratio of Kyn/Trp in tumor tissues (60). As shown in Fig. 5B-D, intratumoral injection of free SHK failed to reduce the Kyn/Trp ratio or PD-L1 expression, while the hydrogel-loaded SHK (SHK@ gel) significantly reduced the ratio of Kyn/Trp (Fig. 5B) and expression of PD-L1 (Fig. 5C and D) in local tumors. It can be inferred that free SHK does not have a retention capacity after intratumoral injection, and it may quickly diffuse to the surrounding tissues or enter the systemic circulation, thus losing its direct and lasting effects on local tumors. Consistent with previous studies (18,61), the repeated stimulation of mild PTT (AuNR@gel + NIR) obviously increased the Kyn/Trp ratio and PD-L1 expression. However, the combination of mild PTT with SHK (SHK/AuNR@gel + NIR) almost completely reversed these phenomena (Fig. 5B-D). In Fig. 5E, it is demonstrated that the effect of AuNR@gel + NIR-mediated mild PTT on ICD induction was not obvious, while SHK@ gel could significantly induce ICD, and the effect on ICD induction was further enhanced when combined with mild PTT (SHK/AuNR@gel + NIR). Furthermore, SHK/AuNR@ gel + NIR almost completely inhibited the growth of the tumor (Fig. 5F and G) and significantly prolonged the survival time of tumor-bearing mice (Fig. 5H). All these results indicate that SHK/AuNR@gel + NIR not only attenuates the increases in Kyn/Trp and PD-L1 expression induced by mild PTT but also has strong abilities to induce ICD and inhibit tumor growth.

To characterize the antitumor immune response elicited by the hydrogel-mediated local SHK delivery combined with mild PTT, a comprehensive analysis of immune-cell activation and cytokine production was performed in TBM (Fig. 6). Within TDLNs, treatment with SHK/AuNR@gel + NIR significantly increased the DC maturation score, defined as the mean z-score of *H2-Ab1*, Cd80, Cd86, Il12a and Il12b expression levels (Fig. 6A and B). This finding indicates enhanced antigen-presenting capacity and improved priming of Th1-oriented immune responses (43,44). Consistent with the pivotal role of IL-12 in promoting Th1 differentiation and IFN-γ production (44), IL-12p70 levels in TDLN homogenates were also significantly elevated following SHK/AuNR@gel + NIR treatment (Fig. 6C).

Analysis of the primary tumors revealed a pronounced increase in T-cell infiltration in the SHK/AuNR@gel + NIR group, as reflected by a significantly higher T-cell infiltration index based on the combined expression of Cd3e, Cd8a and Cd4 (Fig. 6D and E) (45). Moreover, the CYT score, calculated as the geometric mean of Gzmb and Prf1 expression on a logarithmic scale, was substantially increased (Fig. 6F and G) (46), indicating enhanced effector T-cell functionality within the TME. To further assess Treg-associated immunosuppressive features, CD4⁺ TILs were first enriched from primary tumor tissues, followed by CD25 surface labeling-based magnetic enrichment and Foxp3 qPCR analysis (62). AuNR@gel + NIR treatment increased Foxp3 expression in the CD4⁺CD25⁺ Treg-enriched fraction, whereas SHK@gel and SHK/AuNR@ gel + NIR reduced this Foxp3 signal (Fig. 6H). Together with the increased T-cell infiltration and CYT score, these findings suggest that SHK/AuNR@gel + NIR treatment promoted effector T-cell activity while attenuating CD25-enriched Foxp3-associated immunosuppressive features in the primary TME. Finally, levels of the Th1-associated cytokines TNF-α and IFN-γ were significantly elevated in both tumor tissues and serum from mice treated with SHK/AuNR@gel + NIR (Fig. S8A and B; Fig. 6I and J). These findings are indicative of a robust systemic Th1-polarized immune response (44). Taken together, these data demonstrate that SHK/AuNR@gel + NIR effectively remodels the immunosuppressive TME and instigates a potent, systemic adaptive immune response.

Previous studies showed that SHK was injected intraperitoneally every other day for 3 weeks, and no apparent toxic effect was found at the dose of 5.0 mg/kg BW (36,63). In the present study, 5.0 mg/kg were also used as the dosage, but only once (through intratumoral injection). Free SHK, or an equivalent dose of SHK@gel (C_SHK=2 mg/ml, 50 μl) and SHK/AuNR@gel (C_SHK=2 mg/ml, C_AuNR=50 μg/ml, 50 μl) were intratumorally injected into the tumors. The concentrations of SHK in tumor tissues and plasma were quantified by HPLC analysis (Fig. 7A and B). As shown in Fig. 7A, after intratumoral injection, the concentration of free SHK in the tumor rapidly dropped to the trace level in a short time. On the contrary, the concentrations of SHK in the tumor of the two hydrogel groups (SHK@gel or SHK/AuNR@ gel) retained at high levels for a longer time, indicating that the hydrogel could realize the sustained release of SHK and maintain a high concentration in local tumors for a long-lasting time. It should be noted that the local temperature rise mediated by NIR light has no obvious effect on the release profile of SHK from the hydrogel (SHK/AuNR@gel + NIR). After a single intratumoral injection, free SHK rapidly entered the blood circulation, resulting in a sudden increase in SHK in the blood and then a rapid decrease (Fig. 7B). On the contrary, the concentrations of SHK in the blood of the two hydrogel groups did not show this trend and constantly remained at a relatively low level. These findings indicated that the mPECT hydrogel used in the present study can achieve the sustained release of SHK, maintain a relatively high concentration of SHK in the local tumor for a long time, while preventing SHK from rushing into the blood circulation to cause potential damage to normal tissues. In addition, no abnormal changes in blood routine and hepatorenal functions were found among different treatment groups (Fig. 7C and D), and the BW of mice was also not affected during these therapies (Fig. 7E). Furthermore, H&E analysis revealed that no obvious histological damage was observed in the main organs, such as heart, liver, spleen, lung and kidney, after various treatments (Fig. 7F).

To determine whether localized SHK/AuNR@gel + NIR therapy could generate systemic antitumor immunity, a bilateral TBM model was established, in which primary tumors were implanted in the right mammary fat pad and secondary (distant) tumors were implanted in the contralateral mammary fat pad three days later (Fig. 8A). Once the primary tumors reached ~150 mm³, mice received intratumoral injections of the indicated formulations or SHK/AuNR@gel + NIR, followed by NIR irradiation where applicable. Notably, treatment with SHK/AuNR@gel + NIR resulted in pronounced inhibition of distal tumor growth compared with all other groups (Fig. 8B and C). To exclude the possibility that this effect was simply attributable to leakage of SHK from the treated primary tumor and subsequent systemic drug distribution, SHK concentrations in distant tumors were quantified by HPLC following a single intratumoral administration. As revealed in Fig. S9, SHK levels in untreated distant tumors remained extremely low and declined rapidly over time in all treatment groups, remaining well below concentrations associated with direct cytotoxicity. This abscopal effect was accompanied by substantial immune activation within the untreated distal tumors. qPCR analysis revealed significant upregulation of Cd3e, Cd8a and Cd4 in distal tumors from the SHK/AuNR@gel + NIR group (Fig. 8D), resulting in a significantly elevated T-cell infiltration score (Fig. 8E). Similarly, expression levels of the cytolytic effector molecules Gzmb and Prf1 were increased (Fig. 8F), leading to a corresponding increase in the composite CYT score (Fig. 8G). Furthermore, Treg-associated immunosuppressive features in untreated distant tumors were assessed using CD25 surface labeling-based magnetic enrichment of CD4⁺ TILs. Similar to the primary tumors, AuNR@gel + NIR treatment increased Foxp3 expression in the CD4⁺CD25⁺ Treg-enriched fraction, whereas SHK/AuNR@gel + NIR reduced this Foxp3 signal (Fig. 8H). These findings, together with the increased T-cell infiltration and CYT score in distant tumors, support a shift toward a more favorable effector-dominant immune landscape after the combined local treatment. To functionally validate the contribution of T-cell subsets to the abscopal response, in vivo depletion studies were performed. Depletion of either CD8⁺ or CD4⁺ T cells substantially attenuated the ability of SHK/AuNR@gel + NIR treatment to control distant tumor growth (Fig. 8I), demonstrating that both T-cell populations are required for the generation of effective systemic antitumor immunity.

Having established that SHK/AuNR@gel + NIR treatment could suppress both tumor rechallenge and experimental lung metastasis (Fig. 9A-C; and Fig. S10A-C), it was next sought to characterize the functional properties of the long-term immune memory generated by this therapeutic strategy. Splenic T cells were isolated from treated mice 1 day after intravenous challenge with 4T1 cells, as depicted in Fig. 9A, and subjected to a comprehensive panel of ex vivo functional analyses. The antigen specificity of the memory T-cell response was first evaluated. Using an IFN-γ enzyme-linked immunospot (ELISpot) assay, it was found that T cells isolated from SHK/AuNR@gel + NIR-treated mice exhibited a significantly stronger response to 4T1 tumor lysates than those from all other treatment groups, as reflected by a significantly higher number of SFU per 10⁵ T cells (Fig. 9D). The cytotoxic effector function of these memory T cells was next assessed. CD8⁺ T cells were enriched from the splenocytes and co-cultured with irradiated 4T1 target cells at multiple effector-to-target (E:T) ratios in a short-term LDH release assay. CD8⁺ T cells derived from the SHK/AuNR@gel + NIR group displayed potent, dose-dependent tumor cell killing across all E ratios tested. Consistent with this observation, the overall cytotoxic capacity, quantified as the area under the dose-response curve (AUC), was significantly greater than that observed in all control groups (Fig. 9E).

To further elucidate the mechanisms underlying the enhanced cytotoxic activity of memory T cells, the expression of key cytolytic effector molecules was analyzed, and degranulation capacity was assessed. qPCR analysis revealed that the enriched CD8⁺ T cells from the SHK/AuNR@gel + NIR group expressed significantly higher levels of Gzmb and Prf1 (Fig. 9F). Consistent with this finding, antigen-restimulated CD8⁺ T cells from the same group exhibited a significantly greater degree of degranulation, as reflected by the release of these cytolytic effectors upon antigen-specific stimulation (Fig. 9G). Beyond cytotoxic potency, the protective efficacy of memory T cells is strongly influenced by their functional quality, particularly their capacity for polyfunctional cytokine production. To evaluate this property, a dual-color ELISpot assay was employed to quantify T cells capable of simultaneously producing IFN-γ and TNF-α following antigen recall. The frequency of these polyfunctional T cells, expressed as double-positive SFU per 10⁵ T cells, was significantly increased in the SHK/AuNR@gel + NIR group compared with all other treatment groups (Fig. 9H). To obtain a broader overview of the recall immune response, a panel of cytokines and chemokines in the supernatants of antigen-restimulated splenocytes was further quantified. A composite recall-response index was calculated as the mean z-score of IFN-γ, TNF-α, IL-2, CXCL9 and CXCL10 levels. Notably, the SHK/AuNR@gel + NIR group exhibited the highest recall index among all treatment groups (Fig. 9I). Collectively, these findings demonstrate that a single intratumoral administration of SHK/AuNR@gel combined with mild PTT generates a high-quality and durable antitumor immune memory characterized by strong antigen specificity, potent cytotoxic activity, enhanced degranulation, increased T-cell polyfunctionality, and broad cytokine responsiveness.