Expression levels of microRNA-375 in pancreatic cancer

  • Authors:
    • Shiduo Song
    • Jian Zhou
    • Songbing He
    • Dongming Zhu
    • Zixiang Zhang
    • Hua Zhao
    • Yi Wang
    • Dechun Li
  • View Affiliations

  • Published online on: March 27, 2013     https://doi.org/10.3892/br.2013.88
  • Pages: 393-398
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Abstract

MicroRNAs (miRNAs) are small, non‑coding RNAs of endogenous origin that have been increasingly shown to have altered expressions in various cancer types. The expression levels of miR-375 have not been comprehensively investigated in pancreatic cancer. In this study, total RNA was extracted from 44 pairs of pancreatic cancer tissues and non‑tumor adjacent tissues, as well as from four pancreatic cancer cell lines, Panc-1, SW1990, BxpC3 and Patu8988. Following polyadenylation and reverse transcription, the expression levels of miR-375 were determined by real-time PCR and the difference in expression was calculated using the 2-∆∆Ct method. The correlation between the expression levels of miR‑375 and clinicopathological characteristics of pancreatic cancer was also assessed. miR-375 expression was frequently downregulated in the pancreatic cancer tissues compared to their non‑tumor counterparts (P<0.05; paired t-test). Moreover, a significantly low expression of miR-375 was found in the pancreatic cancer cell lines (Panc-1, P=0.016; SW1990, P=0.016; BxPC3, P=0.018; Patu8988, P=0.017; paired t-test). However, no significant correlations were observed between the low expression of miR-375 and parameters including gender, age, tumor size, tumor location and histological grade (P>0.05). The low expression of miR‑375 was correlated with pT stage, lymph node metastases and pTNM stage (P<0.05) (non‑parametric test; Mann-Whitney U test between 2 groups and Kruskal‑Wallis H test for ≥3 groups). In conclusion, miR-375 is potentially involved in the carcinogenesis of pancreatic cancers and serves as is a potential biomarker for pancreatic cancer.
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May-June 2013
Volume 1 Issue 3

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Copy and paste a formatted citation
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Spandidos Publications style
Song S, Zhou J, He S, Zhu D, Zhang Z, Zhao H, Wang Y and Li D: Expression levels of microRNA-375 in pancreatic cancer. Biomed Rep 1: 393-398, 2013
APA
Song, S., Zhou, J., He, S., Zhu, D., Zhang, Z., Zhao, H. ... Li, D. (2013). Expression levels of microRNA-375 in pancreatic cancer. Biomedical Reports, 1, 393-398. https://doi.org/10.3892/br.2013.88
MLA
Song, S., Zhou, J., He, S., Zhu, D., Zhang, Z., Zhao, H., Wang, Y., Li, D."Expression levels of microRNA-375 in pancreatic cancer". Biomedical Reports 1.3 (2013): 393-398.
Chicago
Song, S., Zhou, J., He, S., Zhu, D., Zhang, Z., Zhao, H., Wang, Y., Li, D."Expression levels of microRNA-375 in pancreatic cancer". Biomedical Reports 1, no. 3 (2013): 393-398. https://doi.org/10.3892/br.2013.88