Expression of CD39 mRNA is altered in the peripheral blood of patients with allergic asthma

  • Authors:
    • Lin‑Lin Wang
    • Ping‑Hua Tang
    • Chao‑Guo Shi
    • Ying‑Huan Wan
    • Wei Tang
    • Xia‑Xiao Hou
    • Na‑Li Pan
    • Yu‑Bao Shi
    • Qin‑Lian Tao
  • View Affiliations

  • Published online on: November 6, 2013     https://doi.org/10.3892/br.2013.196
  • Pages: 75-78
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Abstract

The ectoenzyme CD39 hydrolyzes extracellular adenosine 5'-triphosphate (ATP), which possesses pro‑inflammatory properties. However, the role of CD39 in allergic asthma has not been fully elucidated. A total of 18 patients with persistent asthma who were allergic to house dust mites and 19 healthy volunteers were enrolled in this study. The expression of CD39, GATA3, RAR-related orphan receptor γ (ROR‑γt) and forkhead box P3 (Foxp3) mRNA in peripheral blood mononuclear cells (PBMCs) was determined by SYBR‑Green I quantitative polymerase chain reaction (qPCR). The cytokines interleukin (IL)‑4, IL‑17A, transforming growth factor β (TGF‑β) and DP.sIgE were detected by enzyme-linked immunosorbent assay. Our data demonstrated that the expression of CD39 mRNA in PBMCs from asthmatic patients was significantly lower compared to that in normal controls [(1.49±0.59)x10‑3 vs. (2.17±0.77)x10‑3, respectively; P<0.01]. CD39 mRNA was negatively correlated with serum IL‑4, IL‑17A and GATA3 expression (r=‑0.468, P<0.05; r=‑0.550, P<0.05; and r=‑0.424, P<0.01, respectively) and positively correlated with Foxp3 and TGF‑β expression (r=0.373, P<0.05; and r=0.425, P<0.05, respectively). There was no obvious correlation between CD39 and ROR‑γt expression (r=‑0.259, P=0.122). These data suggested that CD39 mRNA expression was downregulated in allergic asthma, which was positively correlated with serum IL‑4, IL‑17A and GATA3 expression and negatively correlated with serum TGF‑β and Foxp3 expression, whereas there was no correlation with ROR‑γt. Therefore, it was hypothesized that CD39 may participate in the occurrence and progression of allergic asthma.
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January-February 2014
Volume 2 Issue 1

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Spandidos Publications style
Wang LL, Tang PH, Shi CG, Wan YH, Tang W, Hou XX, Pan NL, Shi YB and Tao QL: Expression of CD39 mRNA is altered in the peripheral blood of patients with allergic asthma . Biomed Rep 2: 75-78, 2014
APA
Wang, L., Tang, P., Shi, C., Wan, Y., Tang, W., Hou, X. ... Tao, Q. (2014). Expression of CD39 mRNA is altered in the peripheral blood of patients with allergic asthma . Biomedical Reports, 2, 75-78. https://doi.org/10.3892/br.2013.196
MLA
Wang, L., Tang, P., Shi, C., Wan, Y., Tang, W., Hou, X., Pan, N., Shi, Y., Tao, Q."Expression of CD39 mRNA is altered in the peripheral blood of patients with allergic asthma ". Biomedical Reports 2.1 (2014): 75-78.
Chicago
Wang, L., Tang, P., Shi, C., Wan, Y., Tang, W., Hou, X., Pan, N., Shi, Y., Tao, Q."Expression of CD39 mRNA is altered in the peripheral blood of patients with allergic asthma ". Biomedical Reports 2, no. 1 (2014): 75-78. https://doi.org/10.3892/br.2013.196