Open Access

Matrix metalloproteinase‑3 gene promoter polymorphisms: A potential risk factor for pelvic organ prolapse

  • Authors:
    • Charalampos Karachalios
    • Panagiotis Bakas
    • Georgios Kaparos
    • Styliani Demeridou
    • Ilias Liapis
    • Charalampos Grigoriadis
    • Aggelos Liapis
  • View Affiliations

  • Published online on: August 3, 2016     https://doi.org/10.3892/br.2016.736
  • Pages: 337-343
  • Copyright: © Karachalios et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Pelvic organ prolapse (POP) is a common multifactorial condition. Matrix metalloproteinases (MMPs) are enzymes capable of breaking down various connective tissue elements. Single‑nucleotide polymorphisms (SNPs) in regulatory areas of MMP‑encoding genes can alter their transcription rate, and therefore the possible effect on pelvic floor supporting structures. The insertion of an adenine (A) base in the promoter of the MMP‑3 gene at position ‑1612/‑1617 produces a sequence of six adenines (6A), whereas the other allele has five (5A). The aim of the present study was to investigate the possible association of MMP‑3 gene promoter SNPs with the risk of POP. The patient group comprised 80 women with clinically significant POP [Stage II, III or IV; POP quantification (POP‑Q) system]. The control group consisted of 80 females without any or important pelvic floor support defects (Stages 0 or I; POP‑Q system). All the participants underwent the same preoperative evaluation. SNP detection was determined with whole blood sample DNA analysis by quantitative polymerase chain reaction (PCR) in LightCycler® PCR platforms, using the technique of sequence‑specific hybridization probe‑binding assays and melting temperature curve analysis. The results showed there was no statistically significant difference between 5A/5A, 5A/6A and 6A/6A MMP‑3 gene promoter variants in the two study groups (P=0.4758). Therefore, MMP‑3 gene promoter SNPs alone is insufficient to increase the genetic susceptibility to POP development.
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September-2016
Volume 5 Issue 3

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Karachalios C, Bakas P, Kaparos G, Demeridou S, Liapis I, Grigoriadis C and Liapis A: Matrix metalloproteinase‑3 gene promoter polymorphisms: A potential risk factor for pelvic organ prolapse. Biomed Rep 5: 337-343, 2016
APA
Karachalios, C., Bakas, P., Kaparos, G., Demeridou, S., Liapis, I., Grigoriadis, C., & Liapis, A. (2016). Matrix metalloproteinase‑3 gene promoter polymorphisms: A potential risk factor for pelvic organ prolapse. Biomedical Reports, 5, 337-343. https://doi.org/10.3892/br.2016.736
MLA
Karachalios, C., Bakas, P., Kaparos, G., Demeridou, S., Liapis, I., Grigoriadis, C., Liapis, A."Matrix metalloproteinase‑3 gene promoter polymorphisms: A potential risk factor for pelvic organ prolapse". Biomedical Reports 5.3 (2016): 337-343.
Chicago
Karachalios, C., Bakas, P., Kaparos, G., Demeridou, S., Liapis, I., Grigoriadis, C., Liapis, A."Matrix metalloproteinase‑3 gene promoter polymorphisms: A potential risk factor for pelvic organ prolapse". Biomedical Reports 5, no. 3 (2016): 337-343. https://doi.org/10.3892/br.2016.736