Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain

  • Authors:
    • Won Hee Jang
    • Young Joo Jeong
    • Sun Hee Choi
    • Sang‑Hwa Urm
    • Dae‑Hyun Seog
  • View Affiliations

  • Published online on: November 25, 2016     https://doi.org/10.3892/br.2016.818
  • Pages: 46-50
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Abstract

Kinesin 1 is a member of the kinesin superfamily proteins (KIFs) of microtubule-dependent molecular motor proteins that transport organelles and protein complexes in cells. Kinesin 1 consists of a homo‑ or hetero‑dimer of kinesin heavy chains (KHCs), often, although not always, associated with two kinesin light chains (KLCs). KLCs are non‑motor proteins that associate with many different binding proteins and cargoes, but their binding partners have not yet been fully identified. In the present study, a yeast two‑hybrid system was used to identify proteins that interact with the tetratricopeptide repeat (TPR) domain of KLC1. The results of the current study revealed an interaction between the TPR domain of KLC1 and FUN14 domain‑containing protein 1 (FUNDC1), which is a mitochondrial outer membrane protein mediating hypoxia‑induced mitophagy. FUNDC1 bound to the six TPR motif-containing regions of KLC1 and did not interact with KIF5B (a motor subunit of kinesin 1) and KIF3A (a motor subunit of kinesin 2) in the yeast two‑hybrid assay. The cytoplasmic amino N‑terminal domain of FUNDC1 is essential for interaction with KLC1. When co‑expressed in HEK-293T cells, FUNDC1 co‑localized with KLC1 and co-immunoprecipitated with KLC1, but not KIF5B. Collectively, these results indicate that KLC1 may potentially compete with LC3, a key component for autophagosome formation, to interact with FUNDC1.
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January-2017
Volume 6 Issue 1

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Spandidos Publications style
Jang WH, Jeong YJ, Choi SH, Urm SH and Seog DH: Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain. Biomed Rep 6: 46-50, 2017
APA
Jang, W.H., Jeong, Y.J., Choi, S.H., Urm, S., & Seog, D. (2017). Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain. Biomedical Reports, 6, 46-50. https://doi.org/10.3892/br.2016.818
MLA
Jang, W. H., Jeong, Y. J., Choi, S. H., Urm, S., Seog, D."Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain". Biomedical Reports 6.1 (2017): 46-50.
Chicago
Jang, W. H., Jeong, Y. J., Choi, S. H., Urm, S., Seog, D."Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain". Biomedical Reports 6, no. 1 (2017): 46-50. https://doi.org/10.3892/br.2016.818