TY - JOUR AB - Boehmeria nivea (Linn.) Gaudich of the Urticaceae family is a perennial ratoon herbal plant, the root of which is used in traditional Chinese medicine and possesses a variety of pharmacological properties. the 20% ethanol Boehmeria nivea root extract was shown to exert an anti‑hepatitis B virus (HBV) effect in vitro and in vivo; however, whether the Boehmeria nivea leaf (BNL) extract possesses similar properties has not been determined. In this study, we aimed to investigate the anti‑HBV effects of the BNL extract in HepG2.2.15 cells transfected with human HBV DNA. Our results demonstrated that the secretion of HBsAg and HBeAg was reduced in HepG2.2.15 cells treated with the BNL extract, without any recorded cytotoxic effects. In addition, the chloroform fraction (CF) and ethyl acetate fraction (EAF) of BNL were shown to be more potent compared to the other fractions: CF (100 mg̸l) inhibited the secretion of HBsAg by 94.00±1.78% [inhibitory concentration 50 (IC50) = 20.92 mg̸l] and that of HBeAg by 100.19±0.35% (IC50=19.67 mg̸l) after 9 days of treatment. Similarly, EAF (200 mg̸l) inhibited the secretion of HBsAg by 89.95±2.26% (IC50=39.90 mg̸l) and that of HBeAg by 98.90±1.42% (IC50=36.45 mg̸l). Furthermore, we observed that the content of HBV DNA in the medium secreted by the HepG2.2.15 cells was significantly decreased under CF (100 mg̸l) or EAF (200 mg̸l) treatment. Thus, we concluded that the BNL extracts exhibited anti‑HBV activity, with CF and EAF being the most potent among the fractions. AD - Department of Pharmacology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China College of Resources and Environment, Guangxi Normal University, Guilin, Guangxi 541004, P.R. China AU - Wei,Jingchen AU - Lin,Lianku AU - Su,Xiaojian AU - Qin,Shaoyan AU - Xu,Qing AU - Tang,Zunian AU - Deng,Yan AU - Zhou,Yuehan AU - He,Songqing DA - 2014/01/01 DO - 10.3892/br.2013.205 EP - 151 IS - 1 JO - Biomed Rep KW - Boehmeria nivea leaf hepatitis B virus HepG2.2.15 cell HBsAg HBeAg hepatitis B virus DNA PY - 2014 SN - 2049-9434 2049-9442 SP - 147 ST - Anti‑hepatitis B virus activity of Boehmeria nivea leaf extracts in human HepG2.2.15 cells T2 - Biomedical Reports TI - Anti‑hepatitis B virus activity of Boehmeria nivea leaf extracts in human HepG2.2.15 cells UR - https://doi.org/10.3892/br.2013.205 VL - 2 ER -